KEGG   ORTHOLOGY: K27850
Entry
K27850                      KO                                     
Symbol
DBTNBT
Name
3'-N-debenzoyltaxol N-benzoyltransferase [EC:2.3.1.321]
Pathway
map00904  Diterpenoid biosynthesis
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Reaction
R08171  benzoyl-CoA:3'-N-debenzoyltaxol 3'-N-benzoyltransferase
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00904 Diterpenoid biosynthesis
    K27850  DBTNBT; 3'-N-debenzoyltaxol N-benzoyltransferase
Enzymes [BR:ko01000]
 2. Transferases
  2.3  Acyltransferases
   2.3.1  Transferring groups other than aminoacyl groups
    2.3.1.321  3'-N-debenzoyltaxol N-benzoyltransferase
     K27850  DBTNBT; 3'-N-debenzoyltaxol N-benzoyltransferase
Genes
CJF: 131036108 131057048 131061338 131061339 131063160 131064149 131064150 131064151 131064161 131064181 131065005 131072850 131072870 131072871
AG: AAM75818(DBTNBT)
Reference
PMID:12089320
  Authors
Walker K, Long R, Croteau R.
  Title
The final acylation step in taxol biosynthesis: cloning of the taxoid C13-side-chain N-benzoyltransferase from Taxus.
  Journal
Proc Natl Acad Sci U S A 99:9166-71 (2002)
DOI:10.1073/pnas.082115799
  Sequence
[ag:AAM75818]
Reference
PMID:37897038
  Authors
Zhang Y, Wiese L, Fang H, Alseekh S, Perez de Souza L, Scossa F, Molloy J, Christmann M, Fernie AR.
  Title
Synthetic biology identifies the minimal gene set required for paclitaxel biosynthesis in a plant chassis.
  Journal
Mol Plant 16:1951-1961 (2023)
DOI:10.1016/j.molp.2023.10.016
  Sequence
[ag:AAM75818]
LinkDB

KEGG   ENZYME: 2.3.1.321
Entry
EC 2.3.1.321                Enzyme                                 
Name
3'-N-debenzoyltaxol N-benzoyltransferase;
DBTNBT;
3'-N-debenzoyl-2'-deoxytaxol N-benzoyltransferase
Class
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
Sysname
benzoyl-CoA:3'-N-debenzoyltaxol 3'-N-benzoyltransferase
Reaction(IUBMB)
benzoyl-CoA + 3'-N-debenzoyltaxol = CoA + paclitaxel [RN:R08171]
Reaction(KEGG)
R08171
Substrate
benzoyl-CoA [CPD:C00512];
3'-N-debenzoyltaxol [CPD:C16524]
Product
CoA [CPD:C00010];
paclitaxel [CPD:C07394]
Comment
The enzyme, present in Taxus species (yew) catalyses the final step in paclitaxel (Taxol) biosynthesis.
History
EC 2.3.1.321 created 2024
Pathway
ec00904  Diterpenoid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K27850  3'-N-debenzoyltaxol N-benzoyltransferase
Genes
CJF: 131036108 131057048 131061338 131061339 131063160 131064149 131064150 131064151 131064161 131064181 131065005 131072850 131072870 131072871
Reference
1  [PMID:12089320]
  Authors
Walker K, Long R, Croteau R.
  Title
The final acylation step in taxol biosynthesis: cloning of the taxoid C13-side-chain N-benzoyltransferase from Taxus.
  Journal
Proc Natl Acad Sci U S A 99:9166-71 (2002)
DOI:10.1073/pnas.082115799
  Sequence
[ag:AAM75818]
Reference
2  [PMID:18621016]
  Authors
Long RM, Lagisetti C, Coates RM, Croteau RB.
  Title
Specificity of the N-benzoyl transferase responsible for the last step of Taxol biosynthesis.
  Journal
Arch Biochem Biophys 477:384-9 (2008)
DOI:10.1016/j.abb.2008.06.021
Reference
3  [PMID:37897038]
  Authors
Zhang Y, Wiese L, Fang H, Alseekh S, Perez de Souza L, Scossa F, Molloy J, Christmann M, Fernie AR.
  Title
Synthetic biology identifies the minimal gene set required for paclitaxel biosynthesis in a plant chassis.
  Journal
Mol Plant 16:1951-1961 (2023)
DOI:10.1016/j.molp.2023.10.016
Other DBs
ExplorEnz - The Enzyme Database: 2.3.1.321
IUBMB Enzyme Nomenclature: 2.3.1.321
ExPASy - ENZYME nomenclature database: 2.3.1.321
BRENDA, the Enzyme Database: 2.3.1.321
LinkDB

KEGG   REACTION: R08171
Entry
R08171                      Reaction                               
Name
benzoyl-CoA:3'-N-debenzoyltaxol 3'-N-benzoyltransferase
Definition
3'-N-Debenzoyltaxol + Benzoyl-CoA <=> Paclitaxel + CoA
Equation
C16524 + C00512 <=> C07394 + C00010
Reaction class
RC00004  C00010_C00512
RC00064  C07394_C16524
Enzyme
2.3.1.321
Pathway
rn00904  Diterpenoid biosynthesis
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Brite
Enzymatic reactions [BR:br08201]
 2. Transferase reactions
  2.3  Acyltransferases
   2.3.1  Transferring groups other than aminoacyl groups
    2.3.1.321
     R08171  3'-N-Debenzoyltaxol + Benzoyl-CoA <=> Paclitaxel + CoA
Orthology
K27850  3'-N-debenzoyltaxol N-benzoyltransferase [EC:2.3.1.321]
Other DBs
RHEA: 33690
LinkDB

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