KEGG   REACTION: R00033
Entry
R00033                      Reaction                               
Name
thiamine:oxygen 5-oxidoreductase
Definition
Thiamine + Oxygen <=> Hydrogen peroxide + Thiamine aldehyde
Equation
C00378 + C00007 <=> C00027 + C05856
Comment
first step of two-step reaction (see R02132, R00033+R00072)
subsequently R00072 (without release of thiamine aldehyde from enzyme)
Reaction class
RC00087  C00378_C05856
RC02755  C00007_C00027
Enzyme
1.1.3.23
Pathway
rn00730  Thiamine metabolism
rn01100  Metabolic pathways
Brite
Enzymatic reactions [BR:br08201]
 1. Oxidoreductase reactions
  1.1  Acting on the CH-OH group of donors
   1.1.3  With oxygen as acceptor
    1.1.3.23
     R00033  Thiamine + Oxygen <=> Hydrogen peroxide + Thiamine aldehyde
LinkDB

KEGG   REACTION: R00072
Entry
R00072                      Reaction                               
Name
thiamine-aldehyde:oxygen 5-oxidoreductase
Definition
Thiamine aldehyde + Oxygen + H2O <=> Hydrogen peroxide + Thiamine acetic acid
Equation
C05856 + C00007 + C00001 <=> C00027 + C02892
Comment
second step of two-step reaction (see R02132, R00033+R00072)
following after R00033
Reaction class
RC00080  C02892_C05856
RC02755  C00007_C00027
Enzyme
1.1.3.23
Pathway
rn00730  Thiamine metabolism
rn01100  Metabolic pathways
Brite
Enzymatic reactions [BR:br08201]
 1. Oxidoreductase reactions
  1.1  Acting on the CH-OH group of donors
   1.1.3  With oxygen as acceptor
    1.1.3.23
     R00072  Thiamine aldehyde + Oxygen + H2O <=> Hydrogen peroxide + Thiamine acetic acid
Reference
1  [PMID:2988447]
  Authors
Gomez-Moreno C, Edmondson DE.
  Title
Evidence for an aldehyde intermediate in the catalytic mechanism of thiamine oxidase.
  Journal
Arch Biochem Biophys 239:46-52 (1985)
DOI:10.1016/0003-9861(85)90810-0
LinkDB

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