KEGG   Actinoalloteichus hoggarensis: AHOG_15970
Entry
AHOG_15970        CDS       T04975                                 
Symbol
grsB
Name
(GenBank) Gramicidin S synthase 2
  KO
K15395  hybrid polyketide synthase / nonribosomal peptide synthetase FtdB
Organism
ahg  Actinoalloteichus hoggarensis
Brite
KEGG Orthology (KO) [BR:ahg00001]
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   01008 Polyketide biosynthesis proteins [BR:ahg01008]
    AHOG_15970 (grsB)
Polyketide biosynthesis proteins [BR:ahg01008]
 Polyketide synthase (PKS)
  PKS-NRPS hybrid
   Fungal type (iterative)
    AHOG_15970 (grsB)
SSDB
Motif
Pfam: Condensation ketoacyl-synt AMP-binding Acyl_transf_1 KR Ketoacyl-synt_C PS-DH PP-binding PKS_DH_N Thioesterase KAsynt_C_assoc AMP-binding_C Beta-ketacyl_N adh_short adh_short_C2 DUF7080 CurL-like_PKS_C Epimerase Thiolase_N AATase RhiE-like_linker
Other DBs
NCBI-ProteinID: ASO20820
UniProt: A0A221W4K5
LinkDB
Position
complement(3755834..3765370)
AA seq 3178 aa
MTESQYPAARDKVAIIGIGCRLPGSASDYRTFWKNLLDGKDCITPTPADRYDVGSLGSRD
KAKPGRLVGGRGGYIDGFDEFDPAFFGISPREAEHMDPQQRKLLEVAWEALEDGGQKPGD
LAGTDVGVFVGAFTLDYKIVQFADLSFDTLAAHTATGTMMTMVSNRISYCFDFRGPSLSI
DTACSSSLVAVDLACRSLRRGETNLALAGGTLLHMTPQYTIAETKGGFLSPDGRSRTFDA
SANGYVRAEGVGVIALKRLDDALRDGDPIHAVIIGSGVNQDGRTSGITVPNPESQIGLIE
RVCAEAGIAPGDLQYVEAHGTSTPVGDPLEANALGRALAIGREPDSPCYVGSVKTNIGHT
ESAAGIAGLIKVALSLKHRKIPPHINLEHVNPAIDLAALPYEIPTEVTDWPVHDGPARAG
VNAFGFGGTNAHVLLEEAPRQDAEEPIAGDQRPGYSILPLTARDPEAFGDLVRGIQRELA
GVDGVAASLAAVGSTLAHRRQHLESRLSVVYSSRESLDERLAEFLAGEAHPHILLDQVVE
PDRRRLVWAFTGMGPQWWAMGRLLYENEPVYRAAVDECDRELTALTGWSLVAEMNADEAD
SNMAETWLAQPANFAVQVGLAALWRSYGVRPDAVVGHSTGEVAAFYEAGVYSLRDAVTVV
VHRSRLQQKLVDTGTMLAVSLTEDDATRRIESYGDRVSIAAINSPTAMTLAGDEDALAEL
AETLRSEQVFAKFLTVRVPYHSARMDLIKDELLSALADITPRQARVPLYLTAREGRAHGT
ELDASYWWHNVRDSVRFQAAIDRLVDDGHGLFLEIGPHPVLGHSIRECLEARDADGRTLP
SIRRNEDEAARIAMSLASLHNLGVEIDWTALHREGPSVPLPRYPFKRDRYWVEPAPVEQI
RLGRVDHPLLGRRMLNVEPVWESRLDVETTPYLADHRIQGNVVFPAAGYLEMAVQAVRAM
TGGVEATIADVSLRKALFLADDAATTVQFAFSSADAGFTIATVTGDDQEPSVHASGVLRT
GQRRRLTARLDSDAIRARTARHLDAEACYSGLADLGYEYGPAFQCIEEVWVGADEALARI
RPAKVLGDDAAGHHFHPALLDACFQSLLTPQLLDGGREDDSSSTGIRLPLSIDEMRFAPV
GDQPLWVHAVVTRREDDELRGDIVVFADSGVALGRIGGFRAADVEKAATTVGQATIDRWL
TEVGWFDAPLPAEAVDDGRDALGTADPVSADWLVFADGGGVADALAELVTARGGRCHLVR
PGDEYALAPDRRESVVHPGSREDLARLFHELRDGGVPSFGTIVHLWNLDLPSLTDTSRSA
LTAQESLGTYSLIALAQTLAAQDHDDSVAAGRLHVVTRGTQAASPTDVVEPLGAPAWGVG
RVLWNQELIPYRGKLIDLDGSGDRSADARHAEARALLREVSITDEDEIALRADGRRTSRL
VTAEDLTRPLPLRLRPDGSYLVTGAFGALGRLLCRTLVARGARRLILVGRTRLPERDRWH
EVDPDTPAGRGVRLLTELAAAGAETILAPLDITDEAALTTWLAEYRATTSVPIRGVFHLA
GHVRDTLVADMDRAVFDAVHDPKVIGAYLLHHHLRDEPLDHFVLFASVASLLTTAGQTNY
AAGNAFLDALAHHRRAVGLPALSIDWGPWATGMIEELGLIDHYRNSRGMSSLSPDAGMAV
LERVIGQDRAQLLVATVVDWPVFLAWYPTPPTLVAELASSAAEPASDGNSFLDAFRDADE
EKRRLLLTEHFTAVVAAVLRVKTEQVDPAISLGALGLDSLLAMELRARIHTELKVALPVV
ALLSSAPISDAVAQLHDGLVELVAEGEDHAAADAVELYDDERRHPLTQNQTALWFLKQLS
PDGFAYNIGGAVEVRVRLEPELMFQAVRTVIARHPSLRANFTLHEGRPVQVLREVDEIPD
DIALFDVVGRDWDDVYQLIIQEYRKPYDLERDPLIRFRLFKRADDRWVIVKAVHHIVSDA
ISTFTFIEELLEVYEGLREGRRVELVPPSASYLDFLNWQNRFLAGREAQRMLDYWRGHLP
AEVPILNLPTDKPRPVVQTYNGASEFFALDVDLSARVHALAREHDVTVFMVLMSAYYVLL
HRYTGQDDVIVGSPVTGRTQKEFSSVYGYFVNPLPLYVDLAGAPSVTELLAQVRATVLNG
LDNQEYPFVLLVDKLGLQHDPSRSAVFQAMFILLVHKVATEKYGYRLDYIELPEEEGQFD
LTLSVYEEEADQRFHCVFKYNTDLFLPETMRRMASHYVTVLESLTRAPSSTPIHRLDLLG
AEERRRILGEFSGAGRVVEQAVPVHELIGRHAAERPDALAVSLPPAAAGSAETRTSHRLT
YGELDDRAARLAGRLRALGVGPGSVVALCLDKSPDLVVAVLAVLKAGGAYLPLDPDHPTD
RLAFMVRTADAAFAVVDAARRDRLAGLAAADVHTLEDLLDEAASVPPLPAEPVDPAAPAY
LIYTSGSTGRPKAVEVAHHSLATAYDSWRGEYGLDIDVRVHLQMASFSFDVFTGDLVRAL
CSGGTLVLVTRELLFDTERLHRTMLAERVDCGEFVPAVVRALMNFCDHEGLRLDFLRLLI
VGSDAWKVEEYQRLRGLCGPRTRVVNSYGLSEATIDSAYFEGPVDGLEPSQMVPIGRPLP
NSTLYILDEHAQPVPVGVPGELWVGGDGVATGYPSDPEQTARRFVEWSPDHGLIALDATL
ADRPHGGDPRESVDDRPSGTAAAAGPGAEPAAGPMRLYRTGDLARWDAEGRVQLLGRVDG
QVKVRGHRIEIGEIESQLADWPKLAQAVVTVRPDARGEQTLCAYCIPAAGTRLDLRDLRS
HLAERLPTFMIPTHLVELAELPLSPNGKVDLAALPAPSAEHDEQEYEPPVTLYEVRMAEH
WKSLLGLDQVGLRHDFFASGGSSIRLIELIYHLQAEFGISIPVSQLFTVTTLRGMARTVE
HIVTGKLEGARPYLRFNPGQGPTVFCFPPAGGHGLIYRRFAAHLPEHEFVAFNYLPGDDK
VTQYADLIEELQPEGPCPLFGYSLGGNLAFDVAKVLESRGRTVPNVVIMDSYRIPEAFEL
GSERFEAFEKELGEHLRRHTGSEILTEETLEQARDYIRFCSRTPNLGVLDASVSVISDEQ
KVEFYGAAQRGTWHGTSTTSTSVFRGFGSHAEMLDEGFLAGNADLTREILTGGGFSGA
NT seq 9537 nt   +upstreamnt  +downstreamnt
gtgacggaatcacagtaccctgccgcccgcgacaaggtcgcgatcatcggtatcggctgc
cgactgccgggtagcgcctcggactatcgaacgttctggaagaacctgctcgacgggaag
gactgcatcacgccgacaccggcggacagatacgacgtcgggagcctcggcagccgggac
aaggccaaaccaggcaggctggtcgggggcagaggcggctacatcgacggcttcgacgag
ttcgatccggccttcttcgggatcagcccgcgcgaggcggagcacatggacccgcagcag
cgcaagctgctcgaggtggcctgggaggcgctggaggacggcggacagaagcccggtgac
ctcgcgggcaccgacgtcggcgtgttcgtcggggccttcaccctggactacaagatcgtg
cagttcgccgacctctccttcgacacgctggcggcgcacaccgccaccggaacgatgatg
acgatggtgtcgaaccggatctcctactgcttcgacttccggggccccagcctctcgatc
gacaccgcgtgcagctcctcgctggtggcggtggacctggcctgccgcagcctgcgtcgc
ggggagacgaacctggcgctggcgggcggaacgctcctgcacatgacgccgcagtacacg
atcgccgagaccaagggcggcttcctgtcgcccgacgggcgatcccgcaccttcgacgcc
tccgccaacggctacgtccgtgccgagggagtaggtgtgatcgcactcaaacgtctggac
gacgcgctgcgcgacggcgacccgatccacgccgtcatcatcggcagcggcgtcaaccag
gacggccgcaccagcggcatcaccgtgcccaaccccgagtcgcagatcggtctcatcgag
cgggtgtgcgccgaggcgggcatcgcgccgggtgacctgcagtacgtcgaggcgcacggc
acgtccaccccggtcggcgacccgctggaggccaacgcgctcggtcgggcgctggcgatc
ggccgcgaacccgactcgccgtgctacgtcggctcggtcaagaccaacatcggacacacc
gagtcggcggcgggcatcgccgggctcatcaaggtcgccctgagcctgaaacaccggaag
atcccgccgcacatcaacctggagcacgtcaatccggcgatcgacctcgcggcgctgccg
tatgagatccccaccgaggtcaccgactggcccgtgcacgacggaccggcccgcgccggg
gtgaacgccttcggcttcggcggcacgaacgcacacgtcctgctggaggaggcgcctcgc
caggacgccgaggagccgatcgcgggcgaccagcggcccggctactccatcctgccgctg
accgcgcgggacccggaggcgttcggcgacctcgtccgagggatccagcgggaactggcg
ggcgtcgacggcgtggccgcctcactcgccgccgtgggcagcaccctggcgcatcgccgc
cagcacctcgagtcgcggctgtccgtcgtgtactcctcccgcgagtcgctcgacgagcgg
ctggccgagttcctggcgggcgaggcgcatccgcacatcctgctcgaccaggtcgtggag
ccggaccgccgtcgactcgtctgggccttcaccggcatgggaccgcagtggtgggcgatg
ggccgcctgctctacgagaacgagccggtctaccgggccgccgtcgacgagtgcgatcga
gagctgaccgccctcaccggctggtcgctggtcgccgagatgaacgccgacgaggccgac
tcgaacatggccgagacctggctggcacagcccgcgaacttcgccgtccaggtcggtctc
gcggcactctggcggtcctacggcgtgcgacccgacgccgtcgtcgggcacagcaccggt
gaggtcgcggcgttctacgaggcgggcgtctactcgctgcgcgacgccgtcaccgtcgtc
gtgcaccgcagccgactccagcagaagctggtcgacaccggcaccatgctggccgtcagc
ctcaccgaggacgacgcgacccgacggatcgagtcctacggcgaccgggtgtccatcgcg
gcgatcaacagcccgacggccatgacactcgcgggtgacgaggacgccttggccgagctg
gccgagacgctgcgctccgaacaggtcttcgccaagttcctcaccgtccgggtgccctat
cacagcgcgcggatggacctcatcaaggacgagctgctgtccgcgctggccgacatcacg
ccgcggcaggcgcgggtgccgctctacctgaccgcgcgggagggccgggcgcacggcacc
gagctggacgcgagctactggtggcacaacgtccgggacagcgtccgcttccaggcggcg
atcgaccggctggtggacgacggacacggcctcttcctggagatcgggccccatccggtg
ctgggccactcgatccgcgaatgcctcgaggcccgggacgccgacggtcggacgctgccc
tcgatccggcggaacgaggacgaggccgcccggatcgcgatgtcgttggcgagcctgcac
aacctgggcgtcgagatcgactggacggcgctgcaccgcgaagggccgtcggttccgctg
ccccggtatcccttcaaacgcgaccggtactgggtggagcccgcacccgtcgagcagatc
cgcctcggcagggtggaccacccgttgctcggccgtcgcatgctcaacgtcgaaccggtc
tgggagtcccggctggacgtggagacgacgccgtacctggccgaccaccgcatccagggc
aacgtcgtcttcccggcggccggctacctggagatggcggtgcaggcggtccgcgcgatg
accggcggcgtcgaggccaccatcgcggacgtgtcgctgcgcaaggcgctgttcctggcc
gacgacgcggccaccacggtccagttcgccttctccagcgccgacgccggcttcaccatc
gccacggtcaccggcgacgaccaggagccgagcgtgcacgcctcgggggtcctgcgcacc
gggcagcggcggcggctgaccgcccggctggactccgacgcgatccgcgctcgcaccgcc
cgacacctcgacgccgaggcgtgctactcgggcctggccgatctcggctacgagtacggc
ccggcgttccagtgcatcgaggaggtctgggtcggcgccgacgaggctctggcccgtatc
cgccccgcgaaggtactcggcgacgacgccgccgggcatcacttccatccggcgctgctc
gacgcctgcttccagtccctgctcacccctcagcttctcgacggcggccgggaagacgac
tcctcgtccaccggcatccggctgccgctgtcgatcgacgagatgcgcttcgcgcccgtc
ggtgatcaacctctgtgggtgcacgccgtcgtcacccgacgtgaggatgacgaactgcgc
ggcgacatcgtcgtcttcgccgacagcggcgtggcgctgggccggatcggcggcttccgc
gccgccgacgtcgagaaggcggccacgaccgtcgggcaggcgacgatcgaccgctggctg
accgaggtcggctggttcgacgcgccgctgcccgcggaggcggtcgacgacggacgcgac
gccctgggcaccgccgacccggtctccgccgactggctggtcttcgccgacggcggggga
gtggccgacgcactcgccgagctggtcacggcgcggggcggtcgctgtcacctggtacgg
ccgggcgacgagtacgcgctggccccggaccggcgggagtccgtcgtgcacccgggttcc
cgcgaggacctcgcccgactcttccacgagctgcgcgacggcggcgtgccgtccttcggc
accatcgtgcacctctggaacctggacctgccgtcgttgacggacaccagccgctcggcg
ctcaccgcccaggagagcctcggcacgtactcgctcatcgcgctggcgcagaccctggcc
gcgcaggaccacgacgactcggtggcggcgggcaggctgcacgtcgtgacgcggggcacg
caggccgcgtcaccgaccgacgtcgtcgagccgctcggcgcccccgcctggggcgtcgga
cgggtgctgtggaaccaggagctgatcccgtaccggggcaagctcatcgacctcgacggc
tcgggcgaccgctcggcggacgcgcgccacgcggaggcacgggccctgctgcgcgaggtg
tccatcaccgacgaggacgagatcgccctccgggccgacgggcgccgcaccagcagactc
gtcacggccgaggacctgacccgtccgctgccgctgcggctgcggcccgacggcagctac
ctggtcaccggagccttcggcgccctcggcaggctgctgtgtcggaccctcgtcgccagg
ggcgcccgcaggctgatcctggtcggccgcacccgactccccgaacgcgaccgatggcac
gaggtcgacccggacaccccggccggtcgaggcgtgcgcctgctgaccgagctggccgcg
gcgggcgccgagacgatcctcgcaccgctggacatcaccgacgaggcggcgctgacgacg
tggctcgccgagtatcgggcgaccacctccgtgccgatccggggcgtgttccacctcgcc
ggtcacgtgcgcgacaccctcgtcgccgacatggaccgcgccgtcttcgacgccgtccac
gatccgaaggtgatcggcgcctacctcctgcaccaccacctgcgcgacgagccgctcgac
cacttcgtgctcttcgcctcggtggcctcgctgctcaccaccgcgggccagaccaactac
gcggcgggcaacgccttcctcgacgccctggcgcatcaccgacgggcggtggggctgccc
gccctgagcatcgactggggcccgtgggccaccggcatgatcgaggaactcggcctgatc
gaccactaccgcaacagccgagggatgagttcgctgtcccccgacgcgggcatggccgtc
ctcgaacgggtcatcgggcaggatcgggcgcagctgctggtcgccacggtggtggactgg
cccgtcttcctcgcctggtaccccacgccgccgacgctggtggccgagctggcctcctct
gccgccgagcccgcctccgacggcaacagcttcctggacgccttccgcgacgccgacgag
gagaagcggcgactgctgctgaccgagcacttcacggcggtggtcgccgcggtgctgcgg
gtgaagaccgaacaggtcgaccccgcgatcagcctcggcgcgctgggcctggactcgctg
ctggcgatggagctgcgcgccaggattcacaccgaactgaaggtcgcgctgccggtggtg
gcgctgctgagcagcgcgccgatcagcgacgccgtcgcccagcttcacgacggactcgtc
gaactggtcgccgagggcgaggaccacgcggcggccgacgcggtcgagctctacgacgac
gagcgacggcatccgctcacacagaaccagacggcgctgtggttcctcaagcagctcagc
cccgacggcttcgcctacaacatcggcggcgccgtggaggtgcgggtccggctcgaaccg
gagctgatgttccaggcggtgcggacggtgatcgcccgacaccccagcctgcgggccaac
ttcacgctgcacgagggcaggccggtgcaggtgttgcgcgaggtcgacgagatccccgac
gacatcgccctgttcgacgtcgtgggccgggactgggacgacgtctaccagctgatcatc
caggagtaccgcaagccctacgacctcgaacgcgacccgctgatcaggttccggctgttc
aaacgcgccgacgaccgctgggtgatcgtgaaggcggtgcaccacatcgtctccgacgcc
atctcgacgttcaccttcatcgaggagctgctcgaggtgtacgagggcctgcgcgagggg
cgccgggtcgagctggtgccgccgtcggcgagctacctggacttcctcaactggcagaac
cggttcctggcgggccgggaggcacagcggatgctcgactactggcggggccacctgccc
gccgaggtcccgatcctcaacctcccgacggacaagccgcggccggtcgtgcagacctac
aacggggcctcggagttcttcgccctggacgtcgacctgagcgcccgcgtccacgcgctg
gcgcgggagcacgacgtcacggtgttcatggtcctgatgagcgcctactacgtgctgctg
caccgctacaccgggcaggacgacgtcatcgtgggcagcccggtgaccggcaggacccag
aaggagttctcctcggtctacggctacttcgtcaacccgctgccgctgtacgtcgacctg
gcgggtgccccgtcggtcacggagctgctggcgcaggtgcgggccaccgtgctcaacgga
ctcgacaaccaggagtacccgttcgtgctgctggtcgacaagctcgggctgcagcacgat
ccgagccgctccgcggtgttccaggcgatgttcatcctgctcgtccacaaggtcgccacc
gagaagtacggctaccgactggactacatcgagctgcccgaggaggagggccagttcgac
ctcacgctgtcggtctacgaggaggaggccgaccagcgcttccactgtgtcttcaagtac
aacaccgacctcttccttcccgagacgatgcggcgaatggcatcgcactacgtcacggtg
ctggagtcgttgacgcgggcgccctcgtcgacgccgatccaccgtctcgacctgctcgga
gccgaggaacgccgccgcatcctcggcgagttcagcggcgcgggccgcgtcgtcgagcag
gcggtgccggtgcacgagctgatcggcaggcatgccgccgagcgtcccgacgccctcgcc
gtgtcgctgccgcccgccgcggccgggtcggccgagacacggacgtcgcaccggctgacc
tacggcgaactcgacgatcgggccgcccggctggcgggcaggctgcgcgccctgggcgtc
gggcccggctccgtggtggcgctgtgcctggacaagtcgccggacctcgtcgtcgccgtg
ctggcggtgctcaaggcgggcggcgcctacctgcccctggaccccgaccaccccaccgac
cggctggccttcatggtccgcaccgccgacgccgcgttcgccgtcgtcgacgccgcccgg
cgggaccggctcgcgggcctggccgccgccgacgtgcacaccctggaggacctgctcgac
gaggcggcgagcgtaccgccgctgcccgccgaacccgtcgacccggcggcgcccgcctac
ctgatctacacctcgggctccaccggcaggcccaaggcggtggaggtcgcccaccacagc
ctcgccacggcctacgactcctggcgcggcgagtacggcctggacatcgacgtccgggtg
cacctgcagatggcgagcttctccttcgacgtcttcaccggcgacctcgtccgcgcgctg
tgctccgggggcacgctggtcctggtcacacgcgagctgctgttcgacaccgagcggctg
catcggacgatgctcgccgagagggtcgactgcggagagttcgtccccgcggtggtccgg
gccctgatgaacttctgcgaccacgagggactgcgactggacttcctgcgcctgctgatc
gtcggctccgacgcctggaaggtcgaggagtatcagcggcttcgcgggctgtgcgggccg
cggaccagggtggtcaactcctacgggttgagcgaggcgaccatcgacagcgcctacttc
gagggcccggtcgacggtctggagccgagccagatggttcccatcggcaggcccctgccc
aacagcacgctgtacatcctcgacgagcacgcccaaccggtgccggtgggcgtccccggc
gaactgtgggtcggcggggacggcgtggccaccggctatccgagcgacccggagcagacg
gcgcggcgcttcgtcgagtggtcgccggaccacggcctcatcgcactcgatgcgaccctc
gccgaccggccgcacggcggcgatcctcgggagtccgtcgacgaccggccgtcgggaacg
gcggcggccgccgggccgggcgccgagcccgccgccgggccgatgcggttgtaccgcacc
ggggacctggcgcggtgggacgccgaggggcgtgtgcagcttctcggccgggtcgacggc
caggtcaaggtccgcggccatcggatcgagatcggcgagatcgagtcgcagctggccgac
tggccgaagctggcccaggccgtcgtgacggtacgaccggacgccaggggagaacagacc
ctgtgcgcctactgcattccggccgcgggcacgcgactggacctgcgtgacctgcgcagc
cacctcgccgaacggctgccgacgttcatgatccccacgcatctcgtcgagctggcggag
ctgcctctctcgcccaacggcaaggtcgacctcgccgccctgcccgcgccgagcgccgag
cacgacgagcaggagtacgagccgccggtgaccctctacgaggtccggatggccgaacac
tggaagtcgctgctcggcctcgaccaggtggggctgcggcacgacttcttcgcgtcgggc
ggcagttccatccggctgatcgagctgatctaccacctccaggcggagttcggcatcagc
attcccgtcagtcagctgttcaccgtcaccaccctgcgcggcatggccaggaccgtggag
cacatcgtcaccggcaaactcgagggcgcccgcccctacctgcgattcaaccccggccag
ggtccgacggtgttctgcttcccgcccgcgggcggacacggcctgatctaccgccggttc
gccgcccacctcccggaacacgagttcgtcgccttcaactacctgccgggggacgacaag
gtgacgcagtacgcggacctcatcgaggaactgcagcccgagggaccgtgcccgctgttc
ggctactcgctgggcgggaacctcgccttcgacgtcgcgaaggtcctggagtccagggga
cgcaccgtcccgaacgtggtgatcatggactcgtatcggatccccgaggccttcgagttg
ggcagcgagcgcttcgaggccttcgagaaggagctcggcgagcacctgcgcaggcacacc
ggctcggagatcctcacggaggagacgcttgagcaggccagggactacatccgcttctgc
agtcgcacaccgaacctcggcgtcctcgacgcctcggtcagcgtgatctccgacgagcag
aaggtcgagttctacggcgccgcgcagcgcggcacctggcacggcacctcgaccacgagc
acctcggtgttccggggcttcggcagccacgccgagatgctcgacgagggctttctcgcg
ggcaacgccgatctgaccagggagatcctcacaggaggtggattcagtggcgcgtga

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