Escherichia coli BL21(DE3): B21_00036
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Entry
B21_00036 CDS
T02096
Symbol
carB
Name
(GenBank) carB, subunit of carbamoyl phosphate synthetase
KO
K01955
carbamoyl-phosphate synthase large subunit [EC:
6.3.5.5
]
Organism
ebe
Escherichia coli BL21(DE3)
Pathway
ebe00220
Arginine biosynthesis
ebe00240
Pyrimidine metabolism
ebe00250
Alanine, aspartate and glutamate metabolism
ebe01100
Metabolic pathways
ebe01110
Biosynthesis of secondary metabolites
ebe01240
Biosynthesis of cofactors
Module
ebe_M00051
De novo pyrimidine biosynthesis, glutamine (+ PRPP) => UMP
Brite
KEGG Orthology (KO) [BR:
ebe00001
]
09100 Metabolism
09104 Nucleotide metabolism
00240 Pyrimidine metabolism
B21_00036 (carB)
09105 Amino acid metabolism
00250 Alanine, aspartate and glutamate metabolism
B21_00036 (carB)
00220 Arginine biosynthesis
B21_00036 (carB)
Enzymes [BR:
ebe01000
]
6. Ligases
6.3 Forming carbon-nitrogen bonds
6.3.5 Carbon-nitrogen ligases with glutamine as amido-N-donor
6.3.5.5 carbamoyl-phosphate synthase (glutamine-hydrolysing)
B21_00036 (carB)
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Gene cluster
GFIT
Motif
Pfam:
CPSase_L_D2
CPSase_L_D3
MGS
ATP-grasp
Dala_Dala_lig_C
ATP-grasp_3
ATP-grasp_4
ATPgrasp_Ter
CoA_binding_2
BamE
Motif
Other DBs
NCBI-ProteinID:
CAQ30553
UniProt:
A0A140NFY3
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Position
34889..38110
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AA seq
1073 aa
AA seq
DB search
MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRVILVNSNPATIMTDPEM
ADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALELERQGVLEEFGVTMIGATA
DAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAADVGFPCIIRPSFTMGGSGGG
IAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEMEVVRDKNDNCIIVCSIENFDAM
GIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIGVETGGSNVQFAVNPKNGRLIVIEM
NPRVSRSSALASKATGFPIAKVAAKLAVGYTLDELMNDITGGRTPASFEPSIDYVVTKIP
RFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQESLQKALRGLEVGATGFDPKVSLDDPEA
LTKIRRELKDAGAERIWYIADAFRAGLSVDGVFNLTNIDRWFLVQIEELVRLEEKVAEVG
ITGLNAEFLRQLKRKGFADARLAKLAGVREAEIRKLRDQYDLHPVYKRVDTCAAEFATDT
AYMYSTYEEECEANPSTDREKIMVLGGGPNRIGQGIEFDYCCVHASLALREDGYETIMVN
CNPETVSTDYDTSDRLYFEPVTLEDVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVP
VIGTSPDAIDRAEDRERFQHAVERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVL
GGRAMEIVYDEADLRRYFQTAVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIME
HIEQAGVHSGDSACSLPAYTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLI
EVNPRAARTVPFVSKATGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKF
PGVDPLLGPEMRSTGEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDL
AAKLLKQGFELDATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGR
RAIEDSRVIRRSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK
NT seq
3222 nt
NT seq
+upstream
nt +downstream
nt
atgccaaaacgtacagatataaaaagtatcctgattctgggtgcgggcccgattgttatc
ggtcaggcgtgtgagtttgactactctggcgcgcaagcatgtaaagccctgcgcgaagag
ggttaccgcgtcattctggtgaactccaacccggcgaccatcatgaccgacccggaaatg
gccgatgcgacctacatcgagccgattcactgggaagtagtacgcaagattattgaaaaa
gagcgcccggacgcggtgctgccaacgatgggcggtcagacggcgctgaactgcgcgctg
gagctggagcgtcagggcgtgttggaagagttcggcgtgactatgattggtgcgaccgcc
gatgcgattgataaagcagaagaccgccgtcgtttcgacgtagcgatgaagaaaattggt
ctggaaaccgcgcgttccggtatcgcacacacgatggaagaagcgctggcggttgccgct
gacgtgggcttcccgtgcattattcgcccatcctttaccatgggcggtagcggcggcggt
atcgcttataaccgcgaagagtttgaagaaatttgcgcccgcggtctggatctctcccca
accaaagagctgctgattgatgagtcgctgatcggctggaaagagtacgagatggaagtg
gtgcgtgataaaaacgacaactgcatcatcgtctgctctatcgaaaactttgatgcgatg
ggcatccataccggtgactccatcactgtcgcgccagcccaaacgctgaccgacaaagaa
tatcaaatcatgcgtaacgcctcgatggcggtgctgcgtgaaatcggcgttgaaaccggt
ggttccaatgtccagtttgcggtgaacccgaaaaacggtcgcctgattgttatcgaaatg
aacccacgcgtgtcccgttcttcggcgctggcgtcgaaagcgaccggtttcccgattgct
aaagtggcggcgaaactggcggtgggttacaccctcgacgaactgatgaacgacatcact
ggcggacgtactccggcctccttcgagccgtccatcgactatgtggttactaaaattcct
cgcttcaacttcgaaaaattcgccggtgctaacgaccgtctgaccactcagatgaaatcg
gttggcgaagtgatggcgattggtcgcacgcagcaggaatccctgcaaaaagcgctgcgc
ggcctggaagtcggtgcgactggattcgacccgaaagtgagcctcgatgacccggaagcg
ttaaccaaaatccgtcgcgaactgaaagatgctggcgcagagcgtatctggtacatcgcc
gatgccttccgtgcgggcctgtctgtggacggcgtgttcaacctgaccaatattgaccgc
tggttcctggtacagattgaagaactggtgcgtctggaagagaaagtagcggaagtgggc
atcactggcctgaacgctgaattcctgcgccagctgaaacgcaaaggctttgccgatgcg
cgtctggcaaaactggcgggcgtgcgcgaagcggaaatccgcaagctgcgcgaccagtat
gacctgcacccggtttataagcgcgtggatacctgtgcggcagagttcgccaccgacacc
gcttacatgtactccacttatgaagaagagtgcgaagcgaatccgtctaccgaccgtgaa
aaaatcatggttctcggcggcggcccgaaccgtatcggtcagggtatcgaattcgactac
tgctgtgtacacgcctcgctggcgctgcgcgaagacggttacgaaaccattatggttaac
tgtaacccggaaaccgtctccaccgactacgacacttccgatcgcctctacttcgagccg
gtaactctggaagatgtgctggaaatcgtgcgtattgagaagccgaaaggcgttatcgtt
cagtacggtggtcagaccccgctgaaactggcgcgcgcgctggaagctgctggcgtaccg
gttatcggcaccagcccggatgctatcgaccgtgcagaagaccgtgaacgcttccagcat
gcggttgagcgtctgaaactgaaacaaccggcgaacgccaccgttaccgctattgaaatg
gcggtagagaaggcgaaagagattggctacccgctggtggtacgtccgtcttacgttctc
ggcggtcgggcgatggaaatcgtctatgacgaagctgacctgcgtcgctacttccagacg
gcggtcagcgtgtctaacgatgcgccagtgttgctggatcacttccttgatgacgcggta
gaagttgacgtggatgccatctgcgacggcgaaatggtgctgattggcggcatcatggag
cacattgagcaggcgggcgtgcactccggtgactccgcatgttcgctaccagcctacacc
ttaagtcaggaaattcaggatgtgatgcgccagcaggtgcagaaactggccttcgaattg
caggtgcgcggcctgatgaacgtgcagtttgcggttaaaaacaacgaagtctacctgatt
gaagttaacccgcgtgcggcgcgtaccgttccgttcgtctccaaagccaccggcgttccg
ctggcaaaagtggcggcgcgtgtgatggctggcaaatcgctggctgagcagggcgtaacc
aaagaagttatcccgccgtactactcggtgaaagaagtggtgctgccgttcaataaattc
ccgggtgttgacccgctgttagggccagaaatgcgctctaccggggaagtcatgggcgtg
ggccgcaccttcgctgaagcgttcgccaaagcgcagctgggcagcaactccaccatgaag
aaacacggtcgtgcgctgctttccgtgcgcgaaggcgataaagaacgcgtggtggacctg
gcggcaaaactgctgaaacagggcttcgagctggatgcgacgcacggcacggcgattgtg
ctgggtgaagcgggtatcaatccgcgtctggtgaacaaggtgcatgaaggccgtccgcac
attcaggaccgtattaagaatggcgaatatacctacatcatcaacaccacctcaggtcgt
cgtgcgattgaagactcccgcgtgattcgtcgcagtgcgctgcaatataaagtgcattac
gacaccaccctgaacggcggctttgccaccgcgatggcgctgaatgccgatgcgactgaa
aaagtaatttcggtgcaggaaatgcacgcacagatcaaataa
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