Escherichia coli BL21(DE3): B21_00542
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Entry
B21_00542 CDS
T02096
Symbol
entF
Name
(GenBank) apo-serine activating enzyme
KO
K02364
L-serine---[L-seryl-carrier protein] ligase [EC:
6.3.2.14
6.2.1.72
]
Organism
ebe
Escherichia coli BL21(DE3)
Pathway
ebe01053
Biosynthesis of siderophore group nonribosomal peptides
ebe01110
Biosynthesis of secondary metabolites
Brite
KEGG Orthology (KO) [BR:
ebe00001
]
09100 Metabolism
09109 Metabolism of terpenoids and polyketides
01053 Biosynthesis of siderophore group nonribosomal peptides
B21_00542 (entF)
09180 Brite Hierarchies
09181 Protein families: metabolism
01008 Polyketide biosynthesis proteins [BR:
ebe01008
]
B21_00542 (entF)
Enzymes [BR:
ebe01000
]
6. Ligases
6.2 Forming carbon-sulfur bonds
6.2.1 Acid-thiol ligases
6.2.1.72 L-serine---[L-seryl-carrier protein] ligase
B21_00542 (entF)
6.3 Forming carbon-nitrogen bonds
6.3.2 Acid-D-amino-acid ligases (peptide synthases)
6.3.2.14 enterobactin synthase
B21_00542 (entF)
Polyketide biosynthesis proteins [BR:
ebe01008
]
Nonribosomal peptide synthetase (NRPS)
Iterative NRPS
Enterobactin synthetase
B21_00542 (entF)
BRITE hierarchy
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Ortholog
Paralog
Gene cluster
GFIT
Motif
Pfam:
Condensation
AMP-binding
Thioesterase
PP-binding
AMP-binding_C
Prot_ATP_ID_OB_C
Abhydrolase_1
Abhydrolase_6
DUF7096
Hydrolase_4
Lipase_3
Motif
Other DBs
NCBI-ProteinID:
CAQ31059
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All DBs
Position
574638..578519
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AA seq
1293 aa
AA seq
DB search
MSQHLPLVAAQPGIWMAEKLSELPSAWSVAHYVELTGEVDSPLLARAVVAGLAQADTLRM
RFTEDNGEVWQWVDDALTFELPEIIDLRTNIDPHGTAQALMQADLQQDLRVDSGKPLVFH
QLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTWLRGEPTPASPFTPFADVVEE
YQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGRSASADILRLKLEFTDGEFRQLA
TQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFMRRLGSAALTATGPVLNVLPLGIHI
AAQETLPELATRLAAQLKKMRRHQRYDAEQIVRDSGRAAGDEPLFGPVLNIKVFDYQLDI
PGVQAQTHTLATGPVNDLELALFPDEHGDLSIEILANKQRYDEPTLIQHAERLKMLIAQF
AADPALLCGDVDIMLPGEYAQLAQLNATQVEIPETTLSALVAEQAAKTPDAPALADARYL
FSYREMREQVVALANLLRERGVKPGDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYP
DDRLKMMLEDARPSLLITTDDQLPRFSDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYI
IFTSGSTGRPKGVMVGQTAIVNRLLWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIA
GAKLVMAEPEAHRDPLAMQQFFAEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVF
CSGEALPADLCREWQQLTGAPLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVW
NTGLRILDAMMHPVPPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGD
VARWLDNGAVEYLGRSDDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGG
DARQLVGYLVSQSGLPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPL
PELKAQAPGRAPKAGSETIIAAAFSSLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQV
ARQVTPGQVMVASTVAKLATIIDAEEDSTRRMGFETILPLREGNGPTLFCFHPASGFAWQ
FSVLSRYLDPQWSIIGIQSPRPNGPMQTAANLDEVCEAHLATLLEQQPRGPYYLLGYSLG
GTLAQGIAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAA
QQGSTSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIA
ELDIYRQDCAHVDIISPGAFEKIGPIIRATLNR
NT seq
3882 nt
NT seq
+upstream
nt +downstream
nt
atgagccagcatttacctttggtcgccgcacagcccggcatctggatggcagaaaaactg
tcagaattaccctccgcctggagcgtggcgcattacgttgagttaaccggagaggttgat
tcgccattactggcccgcgcggtggttgccggactagcgcaagcagatacgctgcggatg
cgttttacggaagataacggcgaagtctggcagtgggtcgatgatgcgctgacgttcgaa
ctgccagaaattatcgacctgcgaaccaacattgatccgcacggtactgcgcaggcatta
atgcaggccgatttgcagcaagatctgcgcgtcgatagcggtaaaccactggtctttcat
cagctcattcaggtggcagataaccgctggtactggtatcagcgttatcaccatttgctg
gtcgatggctttagtttcccggccattacccgccagatcgccaatatttactgcacatgg
ctgcgtggcgaaccaacgcctgcttcgccgtttacgcctttcgctgatgtagtggaagag
taccagcaataccgcgaaagcgaagcctggcagcgtgatgcggcattctgggcggaacag
cgtcgtcaactgccaccgcccgcgtcactttctccggcacctttaccggggcgcagcgcc
tcggcagatattctgcgcctgaaactggaatttaccgacggggaattccgccagctggct
acgcaactttcaggtgtgcagcgtaccgatttagcccttgcgctggcggctttgtggctg
gggcgattgtgcaaccgcatggactacgctgccggatttatctttatgcgtcgactgggc
tcggcggcgttgacggctaccggacccgtgctcaacgttttgccgttgggtattcatatt
gcggcgcaagaaacgctgccggaactggcaacccgactggcggctcaactgaaaaaaatg
cgtcgtcatcaacgttacgatgccgaacaaattgtccgtgacagcgggcgagcggcaggt
gatgaaccgctgtttggtccggtactcaatatcaaggtatttgattaccaactggatatt
cctggtgttcaggcgcaaacccataccctggcaaccggtccggttaatgaccttgaactg
gccctgttcccggatgaacacggtgatttgagtattgagatcctcgccaataaacagcgt
tacgatgagccaacgttaatccagcatgctgaacgcctgaaaatgctgattgcccagttc
gccgcggatccggcgctgttgtgcggcgatgtcgatattatgctgccaggtgagtatgcg
cagctggcgcagctcaacgccactcaggttgagattccagaaaccacgcttagcgcgctg
gtggcagaacaagcggcaaaaacaccggatgctccggcgctggcagatgcgcgttacctg
ttcagctatcgggaaatgcgcgagcaggtggtggcgctggcgaatctgctgcgtgagcgc
ggcgttaaaccaggggacagcgtggcggtggcactaccgcgctcggtctttttgaccctg
gcactccatgcgatagttgaagctggagcggcctggctaccgctggataccggctatccg
gacgatcgcctgaaaatgatgctggaagatgcgcgtccgtcgctgttaattaccaccgac
gatcaactgccgcgctttagcgatgttcccaatttaacaagcctttgctataacgccccg
cttacaccgcagggcagtgcgccgctgcaactttcacaaccgcatcacacggcttatatc
atctttacctctggctccaccggcaggccgaaaggggtaatggtcgggcagacggctatc
gtcaaccgcctgctttggatgcaaaatcattatccgcttacaggcgaagatgtcgttgcc
caaaaaacgccgtgcagttttgatgtctcggtgtgggagtttttctggccgtttatcgca
ggggcaaaactggtgatggctgaaccggaagcgcaccgcgacccgctcgctatgcagcaa
ttctttgccgaatatggcgtaacgaccacgcactttgtgccgtcgatgctggcggcattt
gttgcctcgctgacgccgcaaaccgctcgccagagttgcgcgacgttgaaacaggttttc
tgtagtggtgaggccttaccggctgatttatgccgcgaatggcaacagttaactggcgcg
ccgttgcataatctatatggcccgacggaagcggcggtagatgtcagctggtatccggct
tttggcgaggaactggcacaggtgcgcggcagcagtgtgccgattggttatccggtatgg
aatacgggtctgcgtattcttgatgcgatgatgcatccggtgccgccgggtgtggcgggt
gatctctatctcactggcattcaactggcgcagggctatctcggacgccccgatctgacc
gccagccgctttattgccgatccttttgccccaggtgaacggatgtaccgtaccggagac
gttgcccgctggctggataacggcgcggtggagtacctcgggcgcagtgatgatcagcta
aaaattcgcgggcagcgtatcgaactgggcgaaatcgatcgcgtgatgcaggcgctgccg
gatgtcgaacaagccgttacccacgcctgtgtgattaaccaggcggctgccaccggtggt
gatgcgcgtcaattggtgggctatctggtgtcgcaatcgggcctgccgttggataccagc
gcattgcaggcgcagcttcgtgaaacattgccaccacatatggtaccggtggttctgctg
caacttccacagttaccacttagcgccaacggcaagctggatcgcaaagccttaccgttg
cctgaactgaaggcacaagcgccagggcgtgcgccgaaagcgggcagtgaaacgattatc
gccgcggcattctcgtcgttgctggggtgtgacgtgcaggatgccgatgctgatttcttc
gcgcttggcggtcattcgctactggcaatgaaactggcagcgcagttaagtcggcaggtt
gcccgccaggtgacgccggggcaagtgatggtcgcgtcaactgtcgccaaactggcaacg
attattgatgctgaagaagacagcacccggcgtatgggattcgaaaccattctgccgttg
cgtgaaggtaatggcccgacgctgttttgtttccatcctgcgtccggttttgcctggcag
ttcagcgtgctctcgcgttatctcgatccacaatggtcgattatcggcattcagtcaccg
cgccccaatggccccatgcagacggcggcaaacctggatgaagtctgcgaagcgcatctg
gcaacgttacttgaacaacaaccgcgcggcccttattacctgctggggtattcgctgggc
ggtacgctggcgcagggtattgcggcgcggctgcgtgcccgtggcgaacaggtggcattt
cttggcttgctggatacctggccgccagaaacgcaaaactggcaggaaaaagaagctaat
ggtctggacccggaagtgctggcggagattaaccgcgaacgcgaggccttcctggcggca
cagcagggaagtacttcaacggagttgtttaccaccattgaaggcaactacgctgatgct
gtgcgcctgctgacgactgctcatagcgtaccgtttgatggtaaagcgacgctgtttgtt
gctgaacgcacgcttcaggaaggtatgagccccgaacgcgcctggtcgccgtggatagcc
gagctggatatctatcgtcaggattgtgcgcatgtggatattatctctccaggggcgttt
gaaaaaattgggccgattattcgcgcaacgctaaacaggtaa
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