Escherichia coli BL21(DE3): ECD_00117
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Entry
ECD_00117 CDS
T00931
Symbol
acnB
Name
(GenBank) aconitate hydratase 2; aconitase B; 2-methyl-cis-aconitate hydratase
KO
K01682
aconitate hydratase 2 / 2-methylisocitrate dehydratase [EC:
4.2.1.3
4.2.1.99
]
Organism
ebl
Escherichia coli BL21(DE3)
Pathway
ebl00020
Citrate cycle (TCA cycle)
ebl00630
Glyoxylate and dicarboxylate metabolism
ebl00640
Propanoate metabolism
ebl00720
Other carbon fixation pathways
ebl01100
Metabolic pathways
ebl01110
Biosynthesis of secondary metabolites
ebl01120
Microbial metabolism in diverse environments
ebl01200
Carbon metabolism
ebl01210
2-Oxocarboxylic acid metabolism
ebl01230
Biosynthesis of amino acids
Module
ebl_M00009
Citrate cycle (TCA cycle, Krebs cycle)
ebl_M00010
Citrate cycle, first carbon oxidation, oxaloacetate => 2-oxoglutarate
ebl_M00012
Glyoxylate cycle
ebl_M00982
Methylcitrate cycle
Brite
KEGG Orthology (KO) [BR:
ebl00001
]
09100 Metabolism
09101 Carbohydrate metabolism
00020 Citrate cycle (TCA cycle)
ECD_00117 (acnB)
00630 Glyoxylate and dicarboxylate metabolism
ECD_00117 (acnB)
00640 Propanoate metabolism
ECD_00117 (acnB)
09102 Energy metabolism
00720 Other carbon fixation pathways
ECD_00117 (acnB)
Enzymes [BR:
ebl01000
]
4. Lyases
4.2 Carbon-oxygen lyases
4.2.1 Hydro-lyases
4.2.1.3 aconitate hydratase
ECD_00117 (acnB)
4.2.1.99 2-methylisocitrate dehydratase
ECD_00117 (acnB)
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Ortholog
Paralog
Gene cluster
GFIT
Motif
Pfam:
Aconitase_2_N
Aconitase_B_N
Aconitase
Motif
Other DBs
NCBI-ProteinID:
ACT42018
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All DBs
Position
134420..137017
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AA seq
865 aa
AA seq
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MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEFLLDLLTNRVPPGVDEA
AYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPLIDALDDAKLAPIAAKALS
HTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRPALAEKLTVTVFKVTGETNTD
DLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVGPIKQIEALQQKGFPLAYVGDVV
GTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGGKIAPIFFNTMEDAGALPIEVDVSN
LNMGDVIDVYPYKGEVRNHETGELLATFELKTDVLIDEVRAGGRIPLIIGRGLTTKAREA
LGLPHSDVFRQAKDVAESDRGFSLAQKMVGRACGVKGIRPGAYCEPKMTSVGSQDTTGPM
TRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVNTHHTLPDFIMNRGGVSLRPGDGVIH
SWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGKM
QPGITLRDLVHAIPLYAIKQGLLTVEKKGKKNIFSGRILEIEGLPDLKVEQAFELTDASA
ERSAAGCTIKLNKEPIIEYLNSNIVLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEA
DADAEYAAVIDIDLADIKEPILCAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAA
GKLLDAHKGQLPTRLWVAPPTRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVA
DGATVVSTSTRNFPNRLGTGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDT
YRYLNFNQLSQYTEKADGVIFQTAV
NT seq
2598 nt
NT seq
+upstream
nt +downstream
nt
gtgctagaagaataccgtaagcacgtagctgagcgtgccgctgaggggattgcgcccaaa
cccctggatgcaaaccaaatggccgcacttgtagagctgctgaaaaacccgcccgcgggc
gaagaagaattcctgttagatctgttaaccaaccgtgttcccccaggcgtcgatgaagcc
gcctatgtcaaagcaggcttcctggctgctatcgctaaaggcgaagccaaatcccctctg
ctgactccggaaaaagccatcgaactgctgggcaccatgcagggtggttacaacattcat
ccgctgatcgacgcgctggatgatgccaaactggcaccgatcgctgccaaagcactttct
cacacactgctgatgttcgataacttctatgacgtagaagagaaagcgaaagcaggcaac
gaatatgcgaagcaggtaatgcagtcctgggcggatgccgaatggttcctgaatcgcccg
gcgctggctgaaaaactgaccgttaccgtcttcaaagtcactggcgaaaccaacaccgat
gacctctctccggcaccggatgcgtggtcacgcccggatatcccactgcacgcgctggcg
atgctgaaaaacgcccgtgaaggcatcgagccagaccagccaggtgttgttggtccgatc
aagcaaatcgaagctctgcaacagaaaggtttcccgctggcgtacgtcggtgacgttgtg
ggtacgggttcatcgcgtaaatccgccacgaactccgtactgtggtttatgggtgatgat
attccacatgtgccgaacaaacgcggcggtggtttgtgcctcggcggtaaaattgcacca
atcttctttaacacaatggaagacgcaggtgcactgccaatcgaagtcgacgtctctaac
ctgaacatgggcgacgtaattgacgtttacccgtacaaaggtgaagtgcgtaaccacgaa
accggcgaactgctggcgaccttcgaactgaaaaccgacgtgctgattgatgaagtgcgt
gctggcggccgtattccgctgattattgggcgtggcctgaccaccaaagcgcgtgaagca
cttggtctgccgcacagtgatgtgttccgtcaggcgaaagatgtcgctgagagcgatcgc
ggcttctcgctggcgcaaaaaatggtaggccgtgcctgtggcgtgaaaggcattcgtccg
ggcgcgtactgcgaaccgaaaatgacttctgtaggctctcaggacaccaccggcccgatg
acccgtgatgaactgaaagacctggcgtgcctgggcttctcggctgacctggtgatgcag
tctttctgccacaccgcggcgtatccgaagccagttgacgtgaacacgcaccacacgctg
ccggacttcattatgaaccgtggcggtgtgtcgctgcgtccgggtgacggcgtcattcac
tcctggctgaaccgtatgctgctgccggataccgtcggtaccggtggtgactcccatacc
cgtttcccgatcggtatctctttcccggcgggttctggtctggtggcgtttgctgccgca
actggcgtaatgccgctggatatgccggaatccgttctggtgcgcttcaaaggcaaaatg
cagccgggcatcaccctgcgcgatctggtacacgcgatcccgctgtatgcgatcaaacaa
ggtctgctgaccgttgagaagaaaggcaagaaaaacatcttctctggccgcatcctggaa
attgaaggtctgccggatctgaaagttgagcaggcctttgagctaaccgatgcgtccgcc
gagcgttctgccgctggttgtaccatcaagctgaacaaagaaccgatcatcgaatacctg
aactctaacatcgtcctgctgaagtggatgatcgcggaaggttacggcgatcgtcgtacc
ctggaacgtcgtattcagggcatggaaaaatggctggcgaatcctgagctgctggaagcc
gatgcagatgcggaatacgcggcagtgatcgacatcgatctggcggatattaaagagcca
atcctctgtgcaccgaacgacccggacgacgcacgtccgctgtctgcggtacagggcgag
aagatcgacgaagtgtttatcggttcctgcatgaccaacatcggtcacttccgtgctgcg
ggtaaactgctggatgcgcacaaaggccagttgccgacgcgcctgtgggtggcaccgcca
acccgtatggatgccgcgcagttgaccgaagaaggctactatagcgtcttcggtaagagc
ggtgcgcgtatcgagatccctggctgttccctgtgtatgggtaaccaggcgcgtgtagca
gacggtgcgacggtggtttccacctctacccgtaacttcccgaaccgtctggggactggc
gcgaatgtcttcctggcttctgcggaactggcggctgttgcggcgctgattggcaaactg
ccgacgccggaagagtaccagacctacgtggcgcaagtagataaaactgccgttgatact
tatcgttatctgaacttcaaccagctttctcagtacaccgaaaaagccgatggggtgatt
ttccagactgcggtttaa
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