Escherichia coli BL21(DE3): ECD_00542
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Entry
ECD_00542 CDS
T00931
Symbol
ybdK
Name
(GenBank) weak gamma-glutamyl:cysteine ligase
KO
K06048
glutamate---cysteine ligase / carboxylate-amine ligase [EC:
6.3.2.2
6.3.-.-]
Organism
ebl
Escherichia coli BL21(DE3)
Pathway
ebl00480
Glutathione metabolism
ebl01100
Metabolic pathways
Brite
KEGG Orthology (KO) [BR:
ebl00001
]
09100 Metabolism
09106 Metabolism of other amino acids
00480 Glutathione metabolism
ECD_00542 (ybdK)
Enzymes [BR:
ebl01000
]
6. Ligases
6.3 Forming carbon-nitrogen bonds
6.3.2 Acid-D-amino-acid ligases (peptide synthases)
6.3.2.2 glutamate---cysteine ligase
ECD_00542 (ybdK)
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GFIT
Motif
Pfam:
GCS2
Glu_cys_ligase
DUF4931_C
Motif
Other DBs
NCBI-ProteinID:
ACT42422
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Position
complement(564934..566052)
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AA seq
372 aa
AA seq
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MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNKITAGEVKHDITESMLE
LATDVCRDINQAAGQFSAMQKVVLQAATDHHLEICGGGTHPFQKWQRQEVCDNERYQRTL
ENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPHFIALSAASPYMQGTDTRFAS
SRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMIDSIKDLHWDIRPSPHFGTVEVRV
MDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKDYLLYKFNRFQACRYGLEGVITDPH
TGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEALHRQVVSGLNEAQLMRDFVADGGSLIG
LVKKHCEIWAGD
NT seq
1119 nt
NT seq
+upstream
nt +downstream
nt
atgccattacccgattttcatgtttctgaaccttttaccctcggtattgaactggaaatg
caggtggttaatccgccgggctatgacttaagccaggactcttcaatgctgattgacgcg
gttaaaaataagatcacggccggagaggtaaagcacgatatcaccgaaagtatgctggag
ctggcgacggatgtttgccgtgatatcaaccaggctgccgggcagttttcagcgatgcag
aaagtcgtattgcaggcagccacagaccatcatctggaaatttgcggcggtggcacgcac
ccgtttcagaaatggcagcgtcaggaggtatgcgataacgaacgttatcaacgcacgctg
gaaaactttggttatctcattcagcaggcgaccgtttttggtcagcatgtccatgttggc
tgcgccagtggcgatgacgccatttatttgctgcacggcttgtcacgatttgtgccgcac
tttatcgccctttccgccgcgtcgccatatatgcagggaacggatacgcgttttgcctcc
tcacgaccgaatattttttccgcctttcctgataatggcccgatgccgtgggtcagtaac
tggcaacaatttgaagccctatttcgctgtttgagttacaccacgatgatcgacagcatt
aaagatctgcactgggatattcgccccagtcctcattttggcacggtggaggttcgggtg
atggataccccgttaacccttagccacgcggtaaatatggcgggattaattcaggccacc
gcccactggttactgacggaacgcccgttcaaacatcaggagaaagattacctgctgtat
aaattcaaccgtttccaggcctgtcgctatgggctggaaggcgtcattaccgatccgcac
actggtgatcgtcgaccgctaacggaagataccttgcgattgctggaaaaaatcgcccct
tccgcacataaaattggcgcatcgagcgcaattgaagcactgcatcgccaggtcgtcagc
ggtctgaatgaagcgcagctgatgcgcgatttcgtcgccgatggcggctcgctgattggg
ctggtgaaaaagcattgtgagatctgggccggtgactaa
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