KEGG   PATHWAY: cmax00908
Entry
cmax00908                   Pathway                                
Name
Zeatin biosynthesis - Cucurbita maxima (winter squash)
Description
Zeatin is a member of the cytokinin family, a class of phytohormones involved in various processes of growth and development in plants. Most abundant cytokinins are adenine-type, where the N6 position of adenine is substituted with an isoprenoid, such as in zeatin, or an aromatic side chain, such as in kinetin. Zeatin can be synthesized in two different pathways: the tRNA pathway and the AMP pathway. In the tRNA pathway zeatin is a recycled product of isopentenylated tRNAs. In the AMP pathway zeatin is synthesized from an isopentenyl donor, dimethylallyl diphosphate (DMAPP), and AMP, ADP, or ATP by isopentenyltransferases. After synthesis cytokinins can be glucosylated.
Class
Metabolism; Metabolism of terpenoids and polyketides
Pathway map
cmax00908  Zeatin biosynthesis
cmax00908

Other DBs
GO: 0009691
Organism
Cucurbita maxima (winter squash) [GN:cmax]
Gene
111497849  adenylate isopentenyltransferase 3, chloroplastic-like [KO:K10760] [EC:2.5.1.27 2.5.1.112]
111480109  adenylate isopentenyltransferase 5, chloroplastic-like [KO:K10760] [EC:2.5.1.27 2.5.1.112]
111486565  adenylate isopentenyltransferase 5, chloroplastic-like [KO:K10760] [EC:2.5.1.27 2.5.1.112]
111486604  adenylate isopentenyltransferase 5, chloroplastic-like [KO:K10760] [EC:2.5.1.27 2.5.1.112]
111499016  adenylate isopentenyltransferase 5, chloroplastic-like [KO:K10760] [EC:2.5.1.27 2.5.1.112]
111475399  adenylate isopentenyltransferase 5, chloroplastic-like [KO:K10760] [EC:2.5.1.27 2.5.1.112]
111467363  adenylate isopentenyltransferase-like [KO:K10760] [EC:2.5.1.27 2.5.1.112]
111470933  adenylate isopentenyltransferase 5, chloroplastic-like [KO:K10760] [EC:2.5.1.27 2.5.1.112]
111472279  adenylate isopentenyltransferase 5, chloroplastic-like [KO:K10760] [EC:2.5.1.27 2.5.1.112]
111488830  adenylate isopentenyltransferase 3, chloroplastic-like [KO:K10760] [EC:2.5.1.27 2.5.1.112]
111489858  adenylate isopentenyltransferase-like [KO:K10760] [EC:2.5.1.27 2.5.1.112]
111494482  adenylate isopentenyltransferase 5, chloroplastic-like [KO:K10760] [EC:2.5.1.27 2.5.1.112]
111470452  adenylate isopentenyltransferase-like [KO:K10760] [EC:2.5.1.27 2.5.1.112]
111466978  adenylate isopentenyltransferase 3, chloroplastic [KO:K10760] [EC:2.5.1.27 2.5.1.112]
111488993  cytokinin hydroxylase-like isoform X1 [KO:K10717]
111487109  cytokinin hydroxylase-like [KO:K10717]
111492562  cytokinin hydroxylase-like [KO:K10717]
111482203  cytokinin hydroxylase-like [KO:K10717]
111465505  cytokinin dehydrogenase 3-like [KO:K00279] [EC:1.5.99.12]
111477202  cytokinin dehydrogenase 7 [KO:K00279] [EC:1.5.99.12]
111485168  cytokinin dehydrogenase 3-like [KO:K00279] [EC:1.5.99.12]
111485172  cytokinin dehydrogenase 3-like [KO:K00279] [EC:1.5.99.12]
111493700  cytokinin dehydrogenase 6-like [KO:K00279] [EC:1.5.99.12]
111482266  cytokinin dehydrogenase 4-like [KO:K00279] [EC:1.5.99.12]
111466785  cytokinin dehydrogenase 2-like [KO:K00279] [EC:1.5.99.12]
111496174  cytokinin dehydrogenase 5-like [KO:K00279] [EC:1.5.99.12]
111485722  cytokinin dehydrogenase 6-like isoform X1 [KO:K00279] [EC:1.5.99.12]
111481206  cytokinin dehydrogenase 7-like isoform X1 [KO:K00279] [EC:1.5.99.12]
111496957  cytokinin dehydrogenase 3-like [KO:K00279] [EC:1.5.99.12]
111465981  cytokinin dehydrogenase 1-like [KO:K00279] [EC:1.5.99.12]
111467189  cytokinin dehydrogenase 1-like [KO:K00279] [EC:1.5.99.12]
111492293  cytokinin dehydrogenase 5-like [KO:K00279] [EC:1.5.99.12]
111498568  UDP-glycosyltransferase 73C3-like [KO:K13496] [EC:2.4.1.-]
111498569  UDP-glycosyltransferase 73C3-like [KO:K13496] [EC:2.4.1.-]
111498570  UDP-glycosyltransferase 73C4-like [KO:K13496] [EC:2.4.1.-]
111481626  UDP-glycosyltransferase 73C1-like [KO:K13496] [EC:2.4.1.-]
111477611  UDP-glycosyltransferase 73C1-like [KO:K13496] [EC:2.4.1.-]
111481633  UDP-glycosyltransferase 73C6-like [KO:K13496] [EC:2.4.1.-]
111498689  UDP-glycosyltransferase 73C6-like [KO:K13496] [EC:2.4.1.-]
111482760  tRNA dimethylallyltransferase 9 [KO:K00791] [EC:2.5.1.75]
111468528  tRNA dimethylallyltransferase 2 isoform X1 [KO:K00791] [EC:2.5.1.75]
111465998  zeatin O-xylosyltransferase-like [KO:K13495] [EC:2.4.1.215]
111467201  zeatin O-glucosyltransferase-like [KO:K13495] [EC:2.4.1.215]
Compound
C00002  ATP
C00008  ADP
C00015  UDP
C00019  S-Adenosyl-L-methionine
C00020  AMP
C00029  UDP-glucose
C00033  Acetate
C00147  Adenine
C00170  5'-Methylthioadenosine
C00190  UDP-D-xylose
C00235  Dimethylallyl diphosphate
C00371  Zeatin
C00979  O-Acetyl-L-serine
C01513  Lupinate
C01804  Discadenine
C02029  Dihydrozeatin
C03300  O-beta-D-Xylosylzeatin
C03423  O-beta-D-Glucosylzeatin
C04083  N6-(Delta2-Isopentenyl)-adenine
C04432  tRNA containing 6-isopentenyladenosine
C04713  N6-(delta2-Isopentenyl)-adenosine 5'-monophosphate
C07330  3-Methyl-2-butenal
C15545  cis-Zeatin
C15546  O-beta-D-Glucopyranosyl-cis-zeatin
C16424  Isopentenyladenosine-5'-triphosphate
C16426  Isopentenyladenosine-5'-diphosphate
C16427  Isopentenyl adenosine
C16428  trans-Zeatin riboside triphosphate
C16429  trans-Zeatin riboside diphosphate
C16430  trans-Zeatin riboside monophosphate
C16431  trans-Zeatin riboside
C16441  cis-Zeatin riboside monophosphate
C16443  trans-Zeatin-7-beta-D-glucoside
C16445  Dihydrozeatin riboside monophosphate
C16447  Dihydrozeatin riboside
C16448  Dihydrozeatin-O-glucoside
C16449  cis-Zeatin riboside
C16467  cis-Prenyl-tRNA
C17324  tRNA adenine
Reference
  Authors
Kasahara H, Takei K, Ueda N, Hishiyama S, Yamaya T, Kamiya Y, Yamaguchi S, Sakakibara H.
  Title
Distinct isoprenoid origins of cis- and trans-zeatin biosyntheses in Arabidopsis.
  Journal
J Biol Chem 279:14049-54 (2004)
DOI:10.1074/jbc.M314195200
Reference
  Authors
Takei K, Sakakibara H, Sugiyama T.
  Title
Identification of genes encoding adenylate isopentenyltransferase, a cytokinin biosynthesis enzyme, in Arabidopsis thaliana.
  Journal
J Biol Chem 276:26405-10 (2001)
DOI:10.1074/jbc.M102130200
Reference
  Authors
Kakimoto T.
  Title
Identification of plant cytokinin biosynthetic enzymes as dimethylallyl diphosphate:ATP/ADP isopentenyltransferases.
  Journal
Plant Cell Physiol 42:677-85 (2001)
DOI:10.1093/pcp/pce112
Reference
  Authors
Takei K, Yamaya T, Sakakibara H.
  Title
Arabidopsis CYP735A1 and CYP735A2 encode cytokinin hydroxylases that catalyze the biosynthesis of trans-Zeatin.
  Journal
J Biol Chem 279:41866-72 (2004)
DOI:10.1074/jbc.M406337200
Reference
  Authors
Hou B, Lim EK, Higgins GS, Bowles DJ.
  Title
N-glucosylation of cytokinins by glycosyltransferases of Arabidopsis thaliana.
  Journal
J Biol Chem 279:47822-32 (2004)
DOI:10.1074/jbc.M409569200
Reference
PMID:9874810
  Authors
Martin RC, Mok MC, Mok DW
  Title
Isolation of a cytokinin gene, ZOG1, encoding zeatin O-glucosyltransferase from Phaseolus lunatus.
  Journal
Proc Natl Acad Sci U S A 96:284-9 (1999)
DOI:10.1073/pnas.96.1.284
Reference
  Authors
Martin RC, Mok MC, Mok DW
  Title
A gene encoding the cytokinin enzyme zeatin O-xylosyltransferase of Phaseolus vulgaris.
  Journal
Plant Physiol 120:553-8 (1999)
DOI:10.1104/pp.120.2.553
Reference
  Authors
Veach YK, Martin RC, Mok DW, Malbeck J, Vankova R, Mok MC
  Title
O-glucosylation of cis-zeatin in maize. Characterization of genes, enzymes, and endogenous cytokinins.
  Journal
Plant Physiol 131:1374-80 (2003)
DOI:10.1104/pp.017210
Reference
  Authors
Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmulling T
  Title
Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity.
  Journal
Plant Cell 15:2532-50 (2003)
DOI:10.1105/tpc.014928
Reference
  Authors
Miyawaki K, Tarkowski P, Matsumoto-Kitano M, Kato T, Sato S, Tarkowska D, Tabata S, Sandberg G, Kakimoto T
  Title
Roles of Arabidopsis ATP/ADP isopentenyltransferases and tRNA isopentenyltransferases in cytokinin biosynthesis.
  Journal
Proc Natl Acad Sci U S A 103:16598-603 (2006)
DOI:10.1073/pnas.0603522103
Related
pathway
cmax00900  Terpenoid backbone biosynthesis
KO pathway
ko00908   
LinkDB

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