KEGG   PATHWAY: psom00563
Entry
psom00563                   Pathway                                
Name
Glycosylphosphatidylinositol (GPI)-anchor biosynthesis - Papaver somniferum (opium poppy)
Description
Cell surface proteins can be attached to the cell membrane via the glycolipid structure called glycosylphosphatidylinositol (GPI) anchor. Hundreds of GPI-anchored proteins have been identified in many eukaryotes ranging from protozoa and fungi to mammals. All protein-linked GPI anchors share a common core structure, characterized by the substructure Man (a1-4) GlcN (a1-6) myo-inositol-1P-lipid. Biosynthesis of GPI anchors proceeds in three stages: (i) preassembly of a GPI precursor in the ER membrane, (ii) attachment of the GPI to the C-terminus of a newly synthesized protein in the lumen of the ER, and (iii) lipid remodeling and/or carbohydrate side-chain modifications in the ER and the Golgi. Defects of GPI anchor biosynthesis gene result in a genetic disorder, paroxysmal nocturnal hemoglobinuria.
Class
Metabolism; Glycan biosynthesis and metabolism
Pathway map
psom00563  Glycosylphosphatidylinositol (GPI)-anchor biosynthesis
psom00563

Other DBs
GO: 0006506
Organism
Papaver somniferum (opium poppy) [GN:psom]
Gene
113302437  phosphatidylinositol N-acetylglucosaminyltransferase subunit A-like [KO:K03857] [EC:2.4.1.198]
113288136  phosphatidylinositol N-acetylglucosaminyltransferase subunit A-like [KO:K03857] [EC:2.4.1.198]
113308101  phosphatidylinositol N-acetylglucosaminyltransferase subunit C-like isoform X1 [KO:K03859]
113338753  phosphatidylinositol N-acetylglucosaminyltransferase subunit C-like isoform X1 [KO:K03859]
113346825  uncharacterized protein LOC113346825 [KO:K03858]
113279064  phosphatidylinositol N-acetylglucosaminyltransferase subunit P-like [KO:K03861]
113302274  phosphatidylinositol N-acetylglucosaminyltransferase subunit P-like [KO:K03861]
113290167  phosphatidylinositol N-acetylglucosaminyltransferase subunit P-like [KO:K03861]
113292914  phosphatidylinositol N-acetylglucosaminyltransferase subunit P-like [KO:K03861]
113299268  phosphatidylinositol N-acetylglucosaminyltransferase subunit GPI1-like [KO:K03860]
113358338  uncharacterized protein LOC113358338 [KO:K03860]
113313669  dolichol-phosphate mannose synthase subunit 2-like [KO:K09658]
113323362  dolichol-phosphate mannose synthase subunit 2 [KO:K09658]
113332652  probable N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase [KO:K03434] [EC:3.5.1.89]
113346804  N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase-like isoform X1 [KO:K03434] [EC:3.5.1.89]
113297111  uncharacterized protein At4g17910-like [KO:K05283] [EC:2.3.-.-]
113302346  uncharacterized protein At4g17910-like isoform X1 [KO:K05283] [EC:2.3.-.-]
113291664  GPI mannosyltransferase 1-like isoform X1 [KO:K05284] [EC:2.4.1.-]
113336745  uncharacterized protein LOC113336745 [KO:K07541]
113304863  phosphatidylinositol-glycan biosynthesis class X protein-like [KO:K07541]
113297417  phosphatidylinositol-glycan biosynthesis class X protein-like [KO:K07541]
113357322  phosphatidylinositol-glycan biosynthesis class X protein-like [KO:K07541]
113301819  GPI mannosyltransferase 2-like [KO:K07542] [EC:2.4.1.-]
113297617  GPI mannosyltransferase 2-like isoform X1 [KO:K07542] [EC:2.4.1.-]
113343704  GPI ethanolamine phosphate transferase 1-like isoform X1 [KO:K05285] [EC:2.7.-.-]
113276385  GPI ethanolamine phosphate transferase 1-like isoform X1 [KO:K05285] [EC:2.7.-.-]
113297789  GPI mannosyltransferase 3-like [KO:K05286] [EC:2.4.1.-]
113301389  GPI mannosyltransferase 3-like isoform X1 [KO:K05286] [EC:2.4.1.-]
113347080  GPI ethanolamine phosphate transferase 3 [KO:K05288] [EC:2.7.-.-]
113326949  uncharacterized protein C1450.15-like [KO:K05287]
113332532  uncharacterized protein C1450.15-like isoform X1 [KO:K05287]
113297846  uncharacterized protein C1450.15-like [KO:K05287]
113280852  uncharacterized protein C1450.15-like [KO:K05287]
113283602  GPI ethanolamine phosphate transferase 2-like isoform X1 [KO:K05310] [EC:2.7.-.-]
113356796  GPI ethanolamine phosphate transferase 2-like [KO:K05310] [EC:2.7.-.-]
113282361  glycosylphosphatidylinositol anchor attachment 1 protein-like [KO:K05289]
113276166  glycosylphosphatidylinositol anchor attachment 1 protein-like [KO:K05289]
113346485  putative GPI-anchor transamidase [KO:K05290]
113356210  putative GPI-anchor transamidase isoform X1 [KO:K05290]
113302239  GPI transamidase component PIG-S-like [KO:K05291]
113297028  GPI transamidase component PIG-S-like [KO:K05291]
113308019  GPI transamidase component PIG-S-like isoform X1 [KO:K05291]
113310502  GPI transamidase component PIG-T homolog [KO:K05292]
113321910  GPI transamidase component PIG-T homolog [KO:K05292]
113272105  phosphatidylinositol glycan anchor biosynthesis class U protein-like [KO:K05293]
113274844  phosphatidylinositol glycan anchor biosynthesis class U protein-like isoform X1 [KO:K05293]
113304684  phosphatidylinositol glycan anchor biosynthesis class U protein-like isoform X1 [KO:K05293]
113348447  uncharacterized protein LOC113348447 [KO:K05294] [EC:3.-.-.-]
113348913  uncharacterized protein LOC113348913 isoform X1 [KO:K05294] [EC:3.-.-.-]
113284402  uncharacterized protein C630.12-like [KO:K23362] [EC:3.1.-.-]
113286885  metallophosphoesterase 1-like [KO:K23362] [EC:3.1.-.-]
113325817  metallophosphoesterase 1-like isoform X1 [KO:K23362] [EC:3.1.-.-]
113355557  uncharacterized protein C630.12-like isoform X1 [KO:K23362] [EC:3.1.-.-]
Compound
C00154  Palmitoyl-CoA
C00346  Ethanolamine phosphate
C00350  Phosphatidylethanolamine
C01194  1-Phosphatidyl-D-myo-inositol
G00143   
G00144   
G00145   
G00146   
G00148   
G00149   
G10610  UDP-N-acetyl-D-glucosamine
G10617  Dolichyl phosphate D-mannose
G12396  6-(alpha-D-glucosaminyl)-1D-myo-inositol
G13044   
G13046   
G13128   
G13150   
G13151   
G13152   
Reference
PMID:12655644
  Authors
Eisenhaber B, Maurer-Stroh S, Novatchkova M, Schneider G, Eisenhaber F.
  Title
Enzymes and auxiliary factors for GPI lipid anchor biosynthesis and post-translational transfer to proteins.
  Journal
Bioessays 25:367-85 (2003)
DOI:10.1002/bies.10254
Reference
PMID:11102867
  Authors
Kinoshita T, Inoue N.
  Title
Dissecting and manipulating the pathway for glycosylphos-phatidylinositol-anchor biosynthesis.
  Journal
Curr Opin Chem Biol 4:632-8 (2000)
DOI:10.1016/S1367-5931(00)00151-4
Reference
PMID:8253761
  Authors
Fankhauser C, Homans SW, Thomas-Oates JE, McConville MJ, Desponds C, Conzelmann A, Ferguson MA.
  Title
Structures of glycosylphosphatidylinositol membrane anchors from Saccharomyces cerevisiae.
  Journal
J Biol Chem 268:26365-74 (1993)
Reference
PMID:14517336
  Authors
Murakami Y, Siripanyapinyo U, Hong Y, Kang JY, Ishihara S, Nakakuma H, Maeda Y, Kinoshita T.
  Title
PIG-W is critical for inositol acylation but not for flipping of glycosylphosphatidylinositol-anchor.
  Journal
Mol Biol Cell 14:4285-95 (2003)
DOI:10.1091/mbc.E03-03-0193
Reference
PMID:10329735
  Authors
Benachour A, Sipos G, Flury I, Reggiori F, Canivenc-Gansel E, Vionnet C, Conzelmann A, Benghezal M.
  Title
Deletion of GPI7, a yeast gene required for addition of a side chain to the glycosylphosphatidylinositol (GPI) core structure, affects GPI protein transport, remodeling, and cell wall integrity.
  Journal
J Biol Chem 274:15251-61 (1999)
DOI:10.1074/jbc.274.21.15251
Reference
PMID:14734546
  Authors
Tanaka S, Maeda Y, Tashima Y, Kinoshita T.
  Title
Inositol deacylation of glycosylphosphatidylinositol-anchored proteins is mediated by mammalian PGAP1 and yeast Bst1p.
  Journal
J Biol Chem 279:14256-63 (2004)
DOI:10.1074/jbc.M313755200
Reference
PMID:12802054
  Authors
Hong Y, Ohishi K, Kang JY, Tanaka S, Inoue N, Nishimura J, Maeda Y, Kinoshita T.
  Title
Human PIG-U and yeast Cdc91p are the fifth subunit of GPI transamidase that attaches GPI-anchors to proteins.
  Journal
Mol Biol Cell 14:1780-9 (2003)
DOI:10.1091/mbc.E02-12-0794
Reference
PMID:12807000
  Authors
Tanaka S, Maeda Y, Kinoshita T.
  Title
[Biosynthesis of GPI-anchored proteins]
  Journal
Tanpakushitsu Kakusan Koso 48:990-6 (2003)
Reference
PMID:15623507
  Authors
Kang JY, Hong Y, Ashida H, Shishioh N, Murakami Y, Morita YS, Maeda Y, Kinoshita T.
  Title
PIG-V involved in transferring the second mannose in glycosylphosphatidylinositol.
  Journal
J Biol Chem 280:9489-97 (2005)
DOI:10.1074/jbc.M413867200
Reference
PMID:15635094
  Authors
Ashida H, Hong Y, Murakami Y, Shishioh N, Sugimoto N, Kim YU, Maeda Y, Kinoshita T.
  Title
Mammalian PIG-X and yeast Pbn1p are the essential components of glycosylphosphatidylinositol-mannosyltransferase I.
  Journal
Mol Biol Cell 16:1439-48 (2005)
DOI:10.1091/mbc.E04-09-0802
Reference
PMID:24364251
  Authors
Fujita M
  Title
[Biosynthesis and remodeling of GPI-anchored proteins].
  Journal
Seikagaku 85:985-95 (2013)
Reference
PMID:25509331
  Authors
Kinoshita T
  Title
[Biochemistry of glycosylphosphatidylinositol (GPI) anchored proteins].
  Journal
Seikagaku 86:626-36 (2014)
Reference
PMID:19837036
  Authors
Fujita M, Maeda Y, Ra M, Yamaguchi Y, Taguchi R, Kinoshita T
  Title
GPI glycan remodeling by PGAP5 regulates transport of GPI-anchored proteins from the ER to the Golgi.
  Journal
Cell 139:352-65 (2009)
DOI:10.1016/j.cell.2009.08.040
Reference
PMID:26563290
  Authors
Kinoshita T, Fujita M
  Title
Biosynthesis of GPI-anchored proteins: special emphasis on GPI lipid remodeling.
  Journal
J Lipid Res 57:6-24 (2016)
DOI:10.1194/jlr.R063313
Related
pathway
psom00510  N-Glycan biosynthesis
psom00564  Glycerophospholipid metabolism
KO pathway
ko00563   
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