Pteropus vampyrus (large flying fox): 105303741
Help
Entry
105303741 CDS
T07815
Name
(RefSeq) serine/threonine-protein kinase SMG1 isoform X1
KO
K08873
serine/threonine-protein kinase SMG1 [EC:
2.7.11.1
]
Organism
pvp
Pteropus vampyrus (large flying fox)
Pathway
pvp03015
mRNA surveillance pathway
Brite
KEGG Orthology (KO) [BR:
pvp00001
]
09120 Genetic Information Processing
09122 Translation
03015 mRNA surveillance pathway
105303741
09180 Brite Hierarchies
09181 Protein families: metabolism
01001 Protein kinases [BR:
pvp01001
]
105303741
09182 Protein families: genetic information processing
03019 Messenger RNA biogenesis [BR:
pvp03019
]
105303741
Enzymes [BR:
pvp01000
]
2. Transferases
2.7 Transferring phosphorus-containing groups
2.7.11 Protein-serine/threonine kinases
2.7.11.1 non-specific serine/threonine protein kinase
105303741
Protein kinases [BR:
pvp01001
]
Serine/threonine kinases: Other
PIKK family [OT]
105303741
Messenger RNA biogenesis [BR:
pvp03019
]
Eukaryotic type
mRNA surveillance and transport factors
Surveillance factors
Other surveillance factors
105303741
mRNA cycle factors
Common to processing body (P body) and stress granule
105303741
BRITE hierarchy
SSDB
Ortholog
Paralog
GFIT
Motif
Pfam:
SMG1
PI3_PI4_kinase
SMG1_N
HEAT_ATR
HEAT
Motif
Other DBs
NCBI-GeneID:
105303741
NCBI-ProteinID:
XP_023377521
UniProt:
A0A6P6BR18
LinkDB
All DBs
Position
Unknown
AA seq
3213 aa
AA seq
DB search
MSRRAPGSRLSSGGGGTKYPRSWNDWQPRTDSASADPDNLKYSSSRDRGGSSSYGLQPSN
SAVVSRQRHDDTRVHADIQNDEKGGYSVNGGSGENTYGRKSMGQELRVNNVTSPEFTSVQ
HGSRALAIKDMRKSQERSMSYSDESRLSNLLRRITREDDRDRRLATVKQLKEFIQQPENK
LVLVKQLDNILAAVHDVLNESSKLLQELRQEGACCLGLLCASLSYEAEKIFKWIFSKFSS
SAKDEVKLLYLCATYKALETVGEKKAFSSVMQLVMTSLQSILENVDTPELLCKCVKCILL
VARCYPHIFSTNFRDTVDILVGWHIDHTQKPSLTQQVSGWLQSLEPFWVADLAFSTTLLG
QFLEDMEAYAEDLSHVASGESVDEDVPPPSVSLPKLAALLRVFSTVVRSIGERFSPIRGP
PITEAYVTDVLYRVMRCVTAANQVFFSEAVLTAANECVGVLLGSLDPSMTIHCDMVITYG
LDQLENCQTCGTDYIISVLNLLTLIVEQINTKLPSSFVEKMFIPSSKLLFLRYHKEKEVV
AVAHAVYQAVLSLKNIPVLETAYKLILGEMTCALNNLLHSLQLPDACSEIKHEAFKNHVF
NVDNAKFVVIFDLSALTTIGNAKNSLIGMWALSPTVFALLSRNLMTVHGDLAVHFPAIQY
AVLYTLYSHCTRHDHFISSSLSSSSPSLFDGAVISTVTTATKKHFSIILNLLGILLKKDN
LNQDTRKLLMTWALEVAVLMKKSETYAPLFSLPSFHKFCKGLLANTLVEDVNICLQACSS
LHALSSSLPDDLLQRCVDVCRVQLVHSGTRIRQAFGKLLKSIPLDVVLSNNNHTEIQEIS
LALRSHMSKAPSNTFHPQDFSDVISFILYGNSHRTGKDNWLERLFYSCQRLDKRDQSTIP
RNLLKTDAVLWQWAIWEAAQFTVLSKLRTPLGRAQDTFQTIEGIIRSLAAHTLNPDQDVS
QWTTADNDEGHSSNQLRLVLLLQYLENLEKLMYNAYEGCANALTSPPKVIRTFFHTNRQT
CQDWLTRIRLSIMRVGLLAGQPAVTVRHGFDLLTEMKTNLSQGNELEVTIMMVVEALCEL
HCPEAIQGIAVWSSSVVGKNLLWINSVAQQAEGRFEKASVEYQEHLCAMTGVDCCISGFD
KSVLTLANAGRNSASPKHALNGDSRKTVLSKTPDSSPEVINYLGNKACECYISIADWAAV
QEWQNAIHDLKKSTSSTSLNLKADFNYIKSLSSFESGEFVECTEQLELLPGENINLLAGG
SKEKIDMKKLLPNMLSPDPRELQKSIEVQLLRSSVCLATALNHIEQDQKWQSVTENVVKY
LKQTSRIAIGPLRLSTLTVSQSLPVLSTLQLYCSSALENTVSNRLSTEDCLIPLFSEALR
SCKQHDVRPWMQALRYTMYQSQLLEKIKEQTVPIRSHLMELGLTAAKFARKRGNVSLATR
LLAQCSEVQLGKTTTAQDLVQHFKKLSTQGQVDEKWGPELDIEKTKLLHTAGQSTHAMEM
LSSCAISFCKSAKAEYAVAKSILTLAKWIQAEWKEISGQLKQVYRAQQQQNLTGLSTLSK
NILTLIELPSVNTMEEEYPRIESESTVHIGVGEPDFILGQLYHLSSVQAPEVAKSWAALA
SWAYRWGRKVVDNASQGEGVRLLPREKSEVQNLLPDTITEEEKERIFGILGQAVCRPAGI
QDEDITLQITESEDNEEDDMVDVIWRQLLSSCPWLSELDESATEGVIKVWRKVVDRIFSL
YKLSCSAYFTFLKLNAGQIPLDEDDPRLHLSHRAEQSTDDVIVMATLRLLRLLVKHAGEL
RQYLEHGLETTPTAPWRGIIPQLFSRLNHPEVYVRQSICNLLCRVAQDSPHLILYPAIVG
TISLSSESQASGNKFSSAIPTLLGNIQGEELLVSECEGGSPPASQDSNKDEPKSGLNEDQ
AMMQDCYSKIVDKLSSANPTMVLQVQMLVAELRRVTVLWDELWLGVLLQQHMYVLRRIQQ
LEDEVKRVQNNNTLRKEEKIAIMREKHTALMKPIVFALEHVRSITAAPAETPHEKWFQDN
YGDAIENALEKLKTPSNPAKPGSSWIPFKEIMLSLQQRAQKRASYILRLEEISPWLAAMT
NTEIALPGEVSARDTVTIHSVGGTITILPTKTKPKKLLFLGSDGKSYPYLFKGLEDLHLD
ERIMQFLSIVNTMFATINRQETPRFHARHYSVTPLGTRSGLIQWVDGATPLFGLYKRWQQ
REAAVQAQKAQDSYQTPQNPGIVPRPSELYYSKIGPALKAVGLSLDVSRRDWPLHVMKAV
LEELMEATPPNLLAKELWSSCTTPDEWWRVTQSYARSTAVMSMVGYIIGLGDRHLDNVLI
DMTTGEVVHIDYNVCFEKGKSLRVPEKVPFRMTQNIETALGVTGVEGVFRLSCEQVLHIM
RRGRETLLTLLEAFVYDPLVDWTAGGEAGFAGAVYGGGGQQAESKQSKREMEREITRSLF
SSRVAEIKVNWFKNRDEMLVVLPKLDSSLDEYLSLQEQLTEVEKLQGKLLEEIEFLEGAE
GVDHPSHTLQHRYSEHTQLQTQQRAVQEAIQVKLNEFEQWITHYQAAFNNLEATQLASLL
QEISTQMDLGPPSYVPATAFLQNAGQAHLISQCEQLEGEVGALLQQRRSVLRGCLEQLHH
YATVALQYPKAIFQKHRIEQWKAWMEELVCGTTVERCQELYRKYEMQYAPQPPPTVCQFI
TATEMTLQRFAADINSRLIRQVERLKQEAVTVPVCEDQLKEIERCIKVFLHENGEEGSLS
LASVIISALCTLTRRNLMMEGAASSAGEQLVDLTSRDGAWFLEELCSMSGNVTCLVQLLK
QCHLVPQDLDIPNPMEASEAVHLANGVYTSLQELNSNFRQIIFPEALRCLMKGEHTLESM
LHELDGLIEQTTDGVPLQTLVESLQAYLRNAAMGLEEETHAHYIDVARILHAQYGELIQP
RNGSVDETPKMSAGQMLLVAFDGMFAQVETAFGLLVEKLNKMEIPVAWRKIDIIREARST
QVNFFDDDNHRQVLEEIFFLKRLQTIKEFFRLCGTFSKTLSGSSSLDDQNTVNGPVQVVS
VKTLFRNSCFSEDQMAKPIKAFTADFVRQLLIGLPNQALGLTLCSFISALGVDIIAQVEA
KDFGAESKVSVDDLCKKAVEHNIQVGKFSQLVMNRATVLASSYDTAWKKHDLVRRLETSI
SSCKTSLQRVQLHIAMFQVRIFLYNVLPLSGGI
NT seq
9642 nt
NT seq
+upstream
nt +downstream
nt
atgagccgcagagccccggggtctcggctgagcagcggcggcggcggcaccaagtacccg
cggagctggaatgactggcaacccagaactgatagtgcatcagccgacccagataattta
aaatattcttcatccagagataggggtgggtcttcctcttatggactgcaaccttcaaat
tcagctgtggtgtctcggcaaaggcacgatgataccagagtccacgctgacatacagaat
gacgaaaagggtggctacagtgtcaatggaggatctggggaaaatacttatggtcggaag
tcgatggggcaagagctgagggttaacaatgtgaccagccctgagttcaccagtgttcag
catggcagtcgtgcgttagccatcaaagacatgaggaaatcacaggagcgatcgatgtct
tattctgatgagtctcgactgtcgaatcttcttcggaggatcacccgggaagatgacaga
gaccggagactggccactgtaaagcagttgaaggaatttattcagcaaccagaaaataaa
ctggtactagttaaacaattggataatatcttggctgctgtacatgatgtgcttaatgaa
agtagcaaattgcttcaggagttgagacaggagggagcttgctgtcttggccttctttgt
gcttctctgagctatgaagctgagaagatcttcaaatggatttttagcaaatttagctca
tctgccaaagatgaagttaaactcctctacttatgtgccacctacaaagcactagagact
gtaggagaaaagaaagccttttcatctgtcatgcagcttgtaatgaccagcctgcagtcc
attcttgaaaatgtggatacaccagaattactttgcaaatgtgttaagtgcattcttttg
gtagctcgatgttaccctcatattttcagcactaattttagggatacagttgatatctta
gttggatggcatatagatcatactcagaaaccttcactcacacagcaggtgtctgggtgg
ttgcagagcctggagcctttttgggtggctgatcttgcattttctaccactctccttggt
cagtttctagaggacatggaggcatatgctgaggacctcagccatgtggcctctggggag
tcagtggatgaagacgtacctcctccgtcagtgtcactaccgaagctggcggcgcttctc
cgggtgttcagcactgtggtgaggagcattggggagcgcttcagccccattcggggccct
ccgatcaccgaggcgtacgtgacggatgttctgtacagagtaatgagatgtgtgacggct
gcaaaccaagtgtttttttctgaggctgtgttgacagctgctaatgagtgtgttggtgtt
ttgctcggcagcttggatcctagcatgactatacattgtgacatggtcattacatatgga
ttagatcaactggagaattgccagacttgtggtacagattatatcatctcagtcttgaat
ttactcacattgattgttgaacagataaatacaaaactgccatcatcatttgtagaaaaa
atgtttataccgtcatctaaactactgtttttgcgttatcataaagaaaaagaggtcgtt
gctgtagcccatgctgtttatcaagcagtgctcagcctgaagaatattcccgttttggag
actgcttataaattaattttgggagaaatgacttgtgccctaaacaacctgctgcatagt
ctgcaacttcctgatgcctgttctgaaataaaacatgaggcttttaagaatcatgtattc
aatgtagacaatgcaaaatttgtagttatatttgacctcagtgccctgactacaatcgga
aatgccaaaaactcattaattgggatgtgggcgctgtctccaactgtcttcgcactcctg
agtaggaatctgatgaccgtgcacggtgacctggctgttcacttccccgccattcagtat
gcagtgctctacactctgtattctcattgtaccaggcacgatcactttatatctagcagc
cttagttcttcgtccccgtctttgttcgatggcgccgtgattagtaccgtaactacagct
acaaaaaaacatttctcaattatcttaaatctcctgggaatattgctaaagaaggataac
cttaaccaggacacaaggaagctgttaatgacttgggctttggaagtggcagttttaatg
aagaagtctgaaacatacgcacctttattctctctcccatctttccataaattttgcaaa
ggccttttagccaacactcttgtggaggatgtgaatatctgtctgcaggcctgcagcagt
ctgcatgctttgtcctcttccctgccggacgatcttttacagaggtgtgttgatgtttgc
cgtgttcaactagtccacagtgggactcgtatccgacaggcttttgggaagctgctgaag
tctattcctttagacgttgtcctgagcaataacaatcacacagaaattcaagaaatttct
ttagcattgagaagtcacatgagtaaagcaccaagtaacacattccatccacaagatttc
tctgatgttattagttttattttatatggaaactcccataggacagggaaggacaattgg
ttagaaagattgttctatagctgccagagactggacaagcgcgaccaatcaacaattccc
cgcaatctcctaaagacagacgctgtcctgtggcagtgggcgatttgggaggcagcacag
ttcactgtcctttccaagctgagaaccccgctgggcagagcacaggacaccttccagaca
atcgaaggtatcattagaagtctcgcagctcacacattaaaccccgatcaagatgtgagt
cagtggacaacagcagacaatgatgaaggccacagtagcaaccagcttaggcttgttctt
cttctacagtatctggaaaatctggagaagttaatgtataatgcatatgagggatgtgcc
aatgcattgacatcacctcccaaggtcattaggacttttttccataccaatcgacaaact
tgtcaagactggctgactcggatccgactctccatcatgagggttggactgttggcaggc
cagcctgctgtgactgtgagacatggctttgacttgcttacagagatgaagaccaatcta
tctcagggaaatgaattggaagtaaccattatgatggtggtagaagcgctgtgtgaactt
cattgtcctgaagctatacagggaattgctgtctggtcgtcatccgttgttgggaaaaat
cttctctggattaactcagtggcccaacaggctgaagggaggtttgaaaaggcctctgtg
gagtaccaggagcatctgtgtgccatgacaggcgtcgattgctgcatctctggcttcgac
aagtctgttctcacgttggccaatgctgggcgaaacagcgccagccccaaacatgctctg
aacggtgactccagaaaaacagtgctgtccaaaacacctgactcttcccctgaggttata
aattatttgggaaacaaggcatgcgagtgctacatatcgattgccgactgggccgctgtg
caggaatggcagaatgccatccacgacctgaagaagagtacgagcagcacttccctcaac
ctcaaagccgatttcaactacataaaatcactaagcagttttgagtctggagaattcgtc
gaatgtactgagcagttagaattgttaccaggagaaaatatcaatctacttgctggagga
tcaaaagaaaaaatagatatgaaaaaactgcttcctaacatgttaagtcccgacccaagg
gaacttcagaaatccattgaagtgcagttgttgagaagttctgtttgtttggcaactgcc
ctgaaccacatagagcaagatcagaagtggcagtctgtgactgaaaatgtggtgaagtac
ttgaagcagacatcccgcatagctattggaccgctgagactttccactttgacagtttcc
cagtctctgccagtgctgagcaccttacagctgtactgctcctctgcgctggagaacacc
gtttctaacagactttctaccgaggactgtctcattccgctcttcagtgaagctttgcgt
tcatgtaaacagcacgatgtgaggccgtggatgcaggcattaagatataccatgtaccag
agtcagttattggagaaaataaaagaacaaacagttccaattagaagccatctcatggaa
ttaggtctaacagcagcaaaatttgctagaaaacggggcaacgtgtcacttgcaacaaga
ctgctggcccagtgtagtgaggttcagctgggaaagaccaccaccgcacaggatttagtc
cagcattttaaaaaactgtcaactcaaggtcaagtggatgaaaaatgggggcctgaactt
gacatcgaaaaaaccaaattgttacatacagcaggccagtcaacacacgcgatggaaatg
ttgagttcctgtgccatatctttctgcaagtctgccaaagccgaatatgcagttgccaag
tcgattctgaccctggctaagtggatccaggcagaatggaaagaaatttcagggcagctg
aagcaggtctacagagctcagcagcagcagaacctcacgggcctttctactttgtctaaa
aacatactcactctgatagaactgccgtctgttaatacgatggaggaggagtatcctcgg
atcgagagtgagtctacagtacatattggagttggagaacctgatttcattttgggacag
ttgtatcacctgtcttcagtacaggcacctgaagtagccaaatcgtgggcagccttggcc
agttgggcttataggtggggcagaaaggtggttgacaatgccagtcagggagaaggtgtt
cgtctgctgcctagagaaaaatctgaagttcaaaatctgcttccagacactataactgaa
gaagaaaaggagagaatatttggtattcttggtcaggctgtgtgtcggccagcggggatt
caggatgaagatataacacttcagataacagaaagcgaagataatgaggaagatgacatg
gtcgatgttatctggcgtcagttactgtcaagctgcccgtggctgtcagaacttgacgaa
agcgcgacagagggcgttatcaaggtgtggaggaaagtggtagatcggatcttcagcctg
tacaaactgtcctgcagcgcatactttactttcctcaagctcaacgctggtcagattcct
ttagacgaagatgacccaaggctgcatttaagtcatagagcagagcagagcaccgatgac
gtgatcgtgatggccacgctgcggttgctcaggctgctcgtgaaacatgccggcgagctc
aggcagtatctggaacatggcctggaaacgacacccactgctccgtggagaggaattatt
ccacagcttttctcacgcttaaatcaccctgaagtgtatgtgcgccaaagcatctgcaac
ctcctctgccgggtggcccaggattccccacacctcatcctgtacccggcaatagtgggt
accatatcgcttagcagtgaatcgcaggcctcaggaaataagttttcctctgcaattcca
actttacttggcaatattcaaggagaagaattgctggtttctgagtgtgagggaggaagt
cctcctgcttctcaggatagcaataaggatgaacctaaaagcggattaaatgaagaccag
gccatgatgcaggattgctacagcaaaattgtagataagctgtcttctgcaaatccgact
atggtgttacaggtgcagatgctcgtggctgagctgcgcagggtcaccgttctctgggat
gagctctggctgggggtcctgttacagcagcacatgtacgtcttgagacggatccagcag
ctcgaagatgaggtgaagagagtccaaaacaacaacaccttacgcaaagaagagaaaatt
gcaatcatgcgagagaagcacacggctctgatgaagcccatcgtgtttgctctggagcac
gtgcgcagcatcactgcggcccctgcagaaacgcctcatgaaaagtggtttcaggataac
tacggagacgcaattgaaaacgcccttgaaaaactgaagacaccctcaaaccctgcaaag
cctggaagcagttggattccatttaaagagataatgctgagtttgcaacagagagcacag
aaacgtgcaagttacatcttgcgtcttgaagaaatcagcccgtggctggctgccatgact
aatactgaaattgctcttcctggagaagtctcagccagagacactgtcacaatccacagc
gtgggcggaaccatcacaatcttacctaccaaaaccaagccaaagaaacttctctttctg
gggtcagatgggaagagctatccttatcttttcaaaggattggaggatttacatctggat
gagagaataatgcagtttctgtctatcgtgaatactatgtttgctacaatcaatcgccag
gaaacaccgcgtttccacgcgcggcactattctgtgacaccgctgggaacgaggtcgggg
ctgatccagtgggtggatggagctacgcccttatttggtctttacaaacggtggcaacag
cgagaagctgccgtacaagcccagaaggcacaagattcctatcaaacccctcagaatcct
ggaattgtgccccgtcctagtgaactttattacagtaaaatcgggcctgctttgaaagcg
gtcggccttagtctggacgtgtcccgacgagattggccccttcatgtgatgaaggcagtg
ttggaagagttaatggaggccacgcccccgaatctccttgctaaagaactttggtcatct
tgcaccacccccgatgaatggtggcgagtcacacagtcgtatgccaggtccaccgcagtc
atgtcgatggtcgggtacataatcggccttggggacagacacctggataacgtgctcata
gacatgacgactggagaggtcgttcacatagattacaacgtctgctttgagaaaggtaaa
agccttcgagttcctgagaaagtgccttttcggatgacacagaacatcgaaacggctctg
ggcgtgactggagtagaaggcgtcttcaggctttcctgcgagcaggttctgcatatcatg
cggcgcggcagggagactctgctgacgctgctggaggccttcgtgtacgacccgctggtg
gactggacggcggggggcgaggccggcttcgctggcgccgtctacggcggaggcggccag
caggccgagagcaagcagagcaagcgggagatggagcgggagatcacccgcagcctcttc
tcctccagagtagctgagattaaggtgaactggtttaagaacagagacgagatgctggtt
gtgctccccaagttggacagcagcttagatgaatacctgagcttgcaggagcagctgacg
gaggtggagaaactgcagggcaagctgctggaagaaatagagtttctggaaggggctgag
ggcgtggatcatccttctcacactctgcaacacaggtattctgaacacacccaactgcag
actcagcaaagggctgttcaggaagcaatccaggtgaagctgaatgaatttgaacagtgg
ataacacattatcaggctgcattcaataatttagaagcaacacagcttgcaagtttgctt
caggagataagcacacaaatggaccttggtcctccaagctacgtgccagcgacagccttt
ctgcagaatgctggtcaggcccacttgatcagtcagtgcgagcagctggagggggaggtg
ggcgcgctgctgcagcagaggcgctcggtgctccgaggctgtctggagcagctgcatcac
tacgcaaccgtggctctgcagtatccgaaggccatattccagaagcaccggatcgagcag
tggaaggcctggatggaggagctggtctgcggcaccaccgtggagcgctgccaggagctc
tacaggaaatatgaaatgcagtatgctccccagccaccgccaacagtgtgtcagttcatc
actgccactgagatgaccctgcagcggttcgcagccgacataaacagcagacttattaga
caggtggagcgcttgaaacaggaagctgtcacggtgcccgtttgtgaagatcagttgaaa
gaaattgaacgttgcattaaagttttccttcacgagaatggagaagaaggctctttgagt
ctagcgagtgttattatttctgccctttgtaccctcacaaggcgtaacctgatgatggaa
ggggcagcttcgagtgcgggagagcagctggtcgatctgacttctcgggatggagcctgg
ttcctggaggagctctgcagtatgagcggaaacgtcacttgcctggttcagttgctgaag
cagtgccacctggtgccccaggacttggacattccgaaccctatggaagcatcggaggca
gttcacttagctaatggagtgtacacctcgcttcaggaattaaattcaaatttccggcaa
atcatatttccagaagcactgagatgtttaatgaaaggagaacacacgttagaaagtatg
cttcatgagctggatggtcttattgagcagaccaccgatggcgttcccctgcagactctc
gtcgaatctcttcaggcctatttaagaaacgcagctatgggacttgaggaagaaacacac
gcgcattacatcgacgtggccagaatacttcatgctcaatatggcgaattaatccaacca
agaaatggttcagttgatgagacaccaaaaatgtcagctggccagatgcttttggtagca
tttgatggcatgtttgcacaagttgaaactgcttttggcttattagttgaaaagttaaac
aagatggaaatccccgtagcctggagaaagatcgacatcatcagggaagcccggagcacc
caggtcaacttttttgatgacgacaatcaccggcaggtgctcgaagagattttctttcta
aaaagactacagacaattaaggagttcttcaggctctgcggcaccttttctaaaactttg
tcaggatccagttcccttgacgatcagaataccgtaaatgggcccgtacaggtcgttagt
gtaaaaaccctgtttcgaaactcctgtttcagtgaagaccagatggccaaacctatcaag
gccttcacagccgacttcgtgcggcagctcctgattgggctgcccaaccaggccctgggc
ctcacgctctgcagcttcatcagtgcgcttggtgtagacatcatcgctcaagtggaggca
aaggattttggtgctgaaagcaaagtttctgtggatgatctgtgtaagaaagccgtggag
cacaacatccaggtcgggaagttctctcagctggtcatgaacagggcgaccgtgctggcc
agttcctacgacactgcctggaagaaacacgacttggtgcgcaggctggaaaccagcatt
tcctcctgtaagacgagcctgcagcgcgttcagctgcacatcgccatgtttcaagtaagg
attttcctttataacgtgcttcccctcagtggaggaatttga
DBGET
integrated database retrieval system
