Shigella flexneri NCTC1 (serotype 2a): NCTC1_00669
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Entry
NCTC1_00669 CDS
T03751
Symbol
miaB
Name
(GenBank) (dimethylallyl)adenosine tRNA methylthiotransferase,(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB,(dimethylallyl)adenosine tRNA methylthiotransferase,tRNA-i(6)A37 thiotransferase enzyme MiaB,Radical SAM superfamily
KO
K06168
tRNA-2-methylthio-N6-dimethylallyladenosine synthase [EC:
2.8.4.3
]
Organism
sft
Shigella flexneri NCTC1 (serotype 2a)
Brite
KEGG Orthology (KO) [BR:
sft00001
]
09180 Brite Hierarchies
09182 Protein families: genetic information processing
03016 Transfer RNA biogenesis [BR:
sft03016
]
NCTC1_00669 (miaB)
Enzymes [BR:
sft01000
]
2. Transferases
2.8 Transferring sulfur-containing groups
2.8.4 Transferring alkylthio groups
2.8.4.3 tRNA-2-methylthio-N6-dimethylallyladenosine synthase
NCTC1_00669 (miaB)
Transfer RNA biogenesis [BR:
sft03016
]
Prokaryotic type
tRNA modification factors
Methylthiotransferases
NCTC1_00669 (miaB)
BRITE hierarchy
SSDB
Ortholog
Paralog
Gene cluster
GFIT
Motif
Pfam:
UPF0004
Radical_SAM
TRAM
TRAM_2
B12-binding
Motif
Other DBs
NCBI-ProteinID:
CDX05928
UniProt:
A0A380B6J3
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All DBs
Position
1:654855..656279
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AA seq
474 aa
AA seq
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MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVF
HQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRG
DRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDD
ILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSFADLLRLVAAIDGIDRIRFTTSH
PIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNLMGRTHTALEYKAIIRKLRAARPDI
QISSDFIVGFPGETTEDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQ
RLYILQERINQQAMAWSRRMLGTTQRILVEGTSRKSIMELSGRTENNRVVNFEGTPDMIG
KFVDVEITDVYPNSLRGKVVRTEDEMGLRMAETPESVIARTRKENDLGVGYYQP
NT seq
1425 nt
NT seq
+upstream
nt +downstream
nt
atgaccaaaaaactccatattaaaacctggggctgtcagatgaacgagtacgattcatcg
aagatggccgatctgctggatgccacccacggctatcaactgaccgacgtggcggaagaa
gcggatgtgctactgctgaacacctgctcaatccgcgagaaggctcaggaaaaagtcttc
catcagttgggtcgctggaaactgttaaaagagaagaatccagacctgattatcggcgtc
ggtggctgcgtggcatcgcaagaaggcgagcacattcgccagcgcgcccactatgtcgat
attatttttgggccgcaaacgctgcaccgtctgccggagatgatcaactccgtgcgcggc
gaccgcagcccggttgtagatatcagcttcccggaaatcgagaagtttgaccgtctgccg
gaaccgcgcgccgaagggccgaccgcgtttgtctccatcatggaaggctgcaataaatat
tgcacctactgcgtggtgccttacacccgtggtgaagaggtaagccgtccgtctgacgat
attctgtttgagattgcccagcttgcggctcagggcgtgcgtgaagtcaacctgctcggt
cagaatgtgaatgcctggcgtggtgagaactacgacggcaccaccggatcgtttgccgat
ctgttgcgtctggttgctgcgatcgacgggatcgatcgtattcgctttaccaccagccat
ccgatcgaattcaccgacgatatcatcgaagtgtaccgcgacacgcctgagctggtgagc
ttcctgcatctgccggtacagagcggttccgatcgcattctgaacctgatgggacgtacc
catacggcgctggagtacaaagcgatcatccgtaaactgcgtgcggcgcgtccggatatt
cagatcagttctgacttcattgttggcttccctggcgaaaccaccgaagacttcgagaaa
acgatgaagctgattgccgacgtcaatttcgacatgagctacagctttatcttctctgca
cgtccaggaacgcctgccgccgatatggttgatgatgttccggaagaagagaagaagcag
cgtctgtatattctgcaagaacgtatcaatcagcaagcgatggcatggagccgtcggatg
ctcggcaccacccagcgtattctggtagaagggacttcgcgtaagagcatcatggagctt
tccgggcgtaccgaaaataaccgcgtggtcaacttcgagggcacaccggatatgatcggt
aaattcgtcgatgtagaaattaccgacgtctacccgaactctctgcgcggtaaagtggtg
cgaactgaagatgaaatgggtctgcgcatggcagaaacaccggagtcggtgattgcccgc
acccgcaaagaaaacgaccttggcgtgggttattatcagccgtaa
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