+F Repair protein KO
#
DNA Repair and Recombination Proteins - Monoraphidium neglectum
%
!
AEukaryotic type
B SSBR (single strand breaks repair)
C Direct repair
D MNEG_7529 methylated-DNA-protein-cysteine methyltransferase K00567 ogt; methylated-DNA-[protein]-cysteine S-methyltransferase [EC:2.1.1.63]
D MNEG_13885 deoxyribodipyrimidine photo-lyase K01669 phr; deoxyribodipyrimidine photo-lyase [EC:4.1.99.3]
C BER (base exicision repair)
D DNA glycosylases
E MNEG_14787 uracil-DNA glycosylase K03648 UNG; uracil-DNA glycosylase [EC:3.2.2.27]
E MNEG_6754 A/G-specific adenine glycosylase K03575 mutY; A/G-specific adenine glycosylase [EC:3.2.2.31]
E MNEG_6094 methyl-CpG binding domain protein 4 K10801 MBD4; methyl-CpG-binding domain protein 4 [EC:3.2.2.-]
E MNEG_8709 hypothetical protein K03660 OGG1; N-glycosylase/DNA lyase [EC:3.2.2.- 4.2.99.18]
E MNEG_5657 endonuclease III K10773 NTHL1; endonuclease III [EC:3.2.2.- 4.2.99.18]
D AP endonucleases
D Long Patch-BER factors
E DNA polymerase delta complex
F MNEG_1145 hypothetical protein K02327 POLD1; DNA polymerase delta subunit 1 [EC:2.7.7.7]
F MNEG_9271 DNA polymerase delta subunit 1 K02327 POLD1; DNA polymerase delta subunit 1 [EC:2.7.7.7]
F MNEG_1736 hypothetical protein K03505 POLD4; DNA polymerase delta subunit 4
E DNA polymerase epsilon complex
F MNEG_12697 hypothetical protein K02325 POLE2; DNA polymerase epsilon subunit 2 [EC:2.7.7.7]
F MNEG_11435 DNA polymerase epsilon subunit 3 K02326 POLE3; DNA polymerase epsilon subunit 3 [EC:2.7.7.7]
E MNEG_1959 Proliferating cell nuclear antigen K04802 PCNA; proliferating cell nuclear antigen
E MNEG_8774 Proliferating cell nuclear antigen K04802 PCNA; proliferating cell nuclear antigen
D Short Patch-BER factors
D Other BER factors
E MNEG_4264 Transcription factor bHLH140 K10863 APTX; aprataxin [EC:3.6.1.70 3.6.1.71 3.6.1.72]
E MNEG_4476 bifunctional polynucleotidephosphatase/kinase K08073 PNKP; bifunctional polynucleotide phosphatase/kinase [EC:3.1.3.32 2.7.1.78]
E MNEG_0586 hypothetical protein K03512 POLL; DNA polymerase lambda [EC:2.7.7.7 4.2.99.-]
C NER (nucleotide excision repair)
D GGR (global genome repair) factors
E XPC-HR23B-CETN2 complex
E Cul4-DDB2 complex
F MNEG_4176 RING-box protein 1 K03868 RBX1; E3 ubiquitin-protein ligase RBX1 [EC:2.3.2.32]
F MNEG_4938 cullin 4 K10609 CUL4; cullin 4
F MNEG_2408 DNA damage-binding protein 1a K10610 DDB1; DNA damage-binding protein 1
F MNEG_10255 Protein DAMAGED DNA-BINDING 2 K10140 DDB2; DNA damage-binding protein 2
E NER4 complex
F MNEG_13186 hypothetical protein K15083 RAD16; DNA repair protein RAD16
D TCR (transcription coupled repair) factors
E DNA-directed RNA polymerase II complex
F MNEG_12639 DNA-directed RNA polymerase II subunit RPB3-A K03011 RPB3; DNA-directed RNA polymerase II subunit RPB3
F MNEG_9076 DNA-directed RNA polymerase II subunit E K03013 RPABC1; DNA-directed RNA polymerases I, II, and III subunit RPABC1
F MNEG_9762 DNA-directed RNA polymerases I, II, and III subunit RPABC2 K03014 RPABC2; DNA-directed RNA polymerases I, II, and III subunit RPABC2
F MNEG_5322 DNA-directed RNA polymerase II subunit RPB7 K03015 RPB7; DNA-directed RNA polymerase II subunit RPB7
F MNEG_7835 DNA-directed RNA polymerases I, II, and III subunit RPABC3 K03016 RPABC3; DNA-directed RNA polymerases I, II, and III subunit RPABC3
F MNEG_12379 hypothetical protein K03017 RPB9; DNA-directed RNA polymerase II subunit RPB9
F MNEG_0306 DNA-directed RNA polymerases I, II, and III subunit RPABC5 K03007 RPABC5; DNA-directed RNA polymerases I, II, and III subunit RPABC5
F MNEG_2353 hypothetical protein K03008 RPB11; DNA-directed RNA polymerase II subunit RPB11
F MNEG_2692 DNA-directed RNA polymerases I, II, and III subunit RPABC4 K03009 RPABC4; DNA-directed RNA polymerases I, II, and III subunit RPABC4
E Cul4-CSA complex
F MNEG_4176 RING-box protein 1 K03868 RBX1; E3 ubiquitin-protein ligase RBX1 [EC:2.3.2.32]
F MNEG_4938 cullin 4 K10609 CUL4; cullin 4
F MNEG_2408 DNA damage-binding protein 1a K10610 DDB1; DNA damage-binding protein 1
E Other TCR factor
F MNEG_8542 Transcription elongation factor 1 K25829 ELOF1; transcription elongation factor 1
D TFIIH complex
E MNEG_0064 DNA excision repair protein ERCC-2 K10844 ERCC2; DNA excision repair protein ERCC-2 [EC:5.6.2.3]
E MNEG_1330 RNA polymerase II transcription factor B subunit 2 K03144 TFIIH4; transcription initiation factor TFIIH subunit 4
E MNEG_8149 hypothetical protein K10845 TTDA; TFIIH basal transcription factor complex TTD-A subunit
E MNEG_5429 Cyclin-H1-1 K06634 CCNH; cyclin H
D RPA (replication factor A)
E MNEG_0168 Replication protein A DNA-binding subunit K07466 RFA1; replication factor A1
E MNEG_1371 replication factor A2 K10739 RFA2; replication factor A2
E MNEG_8716 replication factor A2 K10739 RFA2; replication factor A2
E MNEG_5011 hypothetical protein K10740 RPA3; replication factor A3
D Other NER factors
E MNEG_3211 putative DNA repair endonuclease UVH1 K10848 ERCC4; DNA excision repair protein ERCC-4 [EC:3.1.-.-]
E MNEG_8650 putative MMS19 nucleotide excision repair protein K15075 MET18; DNA repair/transcription protein MET18/MMS19
E MNEG_7296 cullin 3 K03869 CUL3; cullin 3
C MMR (mismatch excision repair)
D Mismatch and loop recognition factors
E MNEG_9220 DNA mismatch repair protein Msh2 K08735 MSH2; DNA mismatch repair protein MSH2
E MNEG_16523 DNA mismatch repair protein MSH2 K08735 MSH2; DNA mismatch repair protein MSH2
E MNEG_14639 DNA mismatch repair protein Msh3 K08736 MSH3; DNA mismatch repair protein MSH3
E MNEG_2780 putative DNA mismatch repair protein Msh6-2 K08737 MSH6; DNA mismatch repair protein MSH6
D MutL homologs
E MNEG_0185 putative MutL protein K08734 MLH1; DNA mismatch repair protein MLH1
D DNA polymerase delta complex
E MNEG_1145 hypothetical protein K02327 POLD1; DNA polymerase delta subunit 1 [EC:2.7.7.7]
E MNEG_9271 DNA polymerase delta subunit 1 K02327 POLD1; DNA polymerase delta subunit 1 [EC:2.7.7.7]
E MNEG_1736 hypothetical protein K03505 POLD4; DNA polymerase delta subunit 4
D RPA (replication factor A)
E MNEG_0168 Replication protein A DNA-binding subunit K07466 RFA1; replication factor A1
E MNEG_1371 replication factor A2 K10739 RFA2; replication factor A2
E MNEG_8716 replication factor A2 K10739 RFA2; replication factor A2
E MNEG_5011 hypothetical protein K10740 RPA3; replication factor A3
D RFC (replication factor C)
E MNEG_8127 replication factor C subunit 2/4 K10755 RFC2_4; replication factor C subunit 2/4
E MNEG_5877 Replication factor C subunit 5 K10756 RFC3_5; replication factor C subunit 3/5
E MNEG_4777 Replication factor C subunit 3 K10756 RFC3_5; replication factor C subunit 3/5
D Other MMR factors
E MNEG_1959 Proliferating cell nuclear antigen K04802 PCNA; proliferating cell nuclear antigen
E MNEG_8774 Proliferating cell nuclear antigen K04802 PCNA; proliferating cell nuclear antigen
E MNEG_7240 exonuclease 1 K10746 EXO1; exonuclease 1 [EC:3.1.-.-]
E MNEG_6085 hypothetical protein K24755 WDR76; WD repeat-containing protein 76
B DSBR (double strand breaks repair)
C HR (homologous recombination)
D MRN(MRX) complex
D BRCA1-core complex
D BRCA1-A complex
D BRCA1-B complex
E MNEG_3590 Fanconi anemia group J protein K15362 BRIP1; fanconi anemia group J protein [EC:5.6.2.3]
D BRCA1-C complex
D BRCA complex
E MNEG_12354 hypothetical protein K08775 BRCA2; breast cancer 2 susceptibility protein
E MNEG_7488 putative DNA repair protein RAD51 like protein K04482 RAD51; DNA repair protein RAD51
D RecA family proteins
E MNEG_7488 putative DNA repair protein RAD51 like protein K04482 RAD51; DNA repair protein RAD51
E MNEG_5735 RAD51-like protein 2 K10870 RAD51L2; RAD51-like protein 2
D Rad52 family proteins
D Rad54 family proteins
E MNEG_6158 DNA repair and recombination protein RAD54 and RAD54-like protein K10875 RAD54L; DNA repair and recombination protein RAD54 and RAD54-like protein [EC:5.6.2.-]
E MNEG_10899 DNA repair and recombination protein RAD54 and RAD54-like protein K10875 RAD54L; DNA repair and recombination protein RAD54 and RAD54-like protein [EC:5.6.2.-]
E MNEG_5964 DNA repair and recombination protein RAD54B K10877 RAD54B; DNA repair and recombination protein RAD54B [EC:5.6.2.-]
D RecQ family DNA helicases
E MNEG_4232 ATP-dependent DNA helicase Q1 K10899 RECQL; ATP-dependent DNA helicase Q1 [EC:5.6.2.4]
D Bloom's syndrome complex (BTR)
E MNEG_8196 topoisomerase (DNA) III beta K03165 TOP3; DNA topoisomerase III [EC:5.6.2.1]
D RPA (replication factor A)
E MNEG_0168 Replication protein A DNA-binding subunit K07466 RFA1; replication factor A1
E MNEG_1371 replication factor A2 K10739 RFA2; replication factor A2
E MNEG_8716 replication factor A2 K10739 RFA2; replication factor A2
E MNEG_5011 hypothetical protein K10740 RPA3; replication factor A3
D Protein phosphatase 4
D AP-5 complex
D SMC5-SMC6 complex
E MNEG_1167 Structural maintenance of chromosomes protein 5 K22803 SMC5; structural maintenance of chromosomes protein 5
E MNEG_6995 hypothetical protein K22756 NSMCE2; E3 SUMO-protein ligase NSE2 [EC:2.3.2.-]
E MNEG_7048 hypothetical protein K22825 NSMCE4; non-structural maintenance of chromosomes element 4
E MNEG_2576 hypothetical protein K22825 NSMCE4; non-structural maintenance of chromosomes element 4
D Other HR factors
E MNEG_1343 hypothetical protein K10881 SHFM1; 26 proteasome complex subunit DSS1
E MNEG_9874 hypothetical protein K10881 SHFM1; 26 proteasome complex subunit DSS1
E MNEG_0484 hypothetical protein K15078 SLX1; structure-specific endonuclease subunit SLX1 [EC:3.6.1.-]
E MNEG_2668 putative Flap endonuclease GEN K15338 GEN1; holliday junction resolvase GEN1/YEN1 [EC:3.1.-.-]
E MNEG_10779 hypothetical protein K22544 SAMHD1; deoxynucleoside triphosphate triphosphohydrolase SAMHD1 [EC:3.1.5.-]
E MNEG_8152 SAM domain and HD domain-containing protein 1 K22544 SAMHD1; deoxynucleoside triphosphate triphosphohydrolase SAMHD1 [EC:3.1.5.-]
E MNEG_15600 vacuolar protein-sorting-associated protein 4 K22766 FIGNL1; fidgetin-like protein 1 [EC:5.6.2.-]
E MNEG_13989 Protein SAP1 K22766 FIGNL1; fidgetin-like protein 1 [EC:5.6.2.-]
E MNEG_6627 hypothetical protein K22766 FIGNL1; fidgetin-like protein 1 [EC:5.6.2.-]
E MNEG_10765 DNA damage response protein WSS1 K22685 WSS1; DNA-dependent metalloprotease WSS1 [EC:3.4.24.-]
C NHEJ (non-homologous end-joining)
D DNA-PK complex
E MNEG_6042 ATP-dependent DNA helicase 2 subunit 2 K10885 XRCC5; ATP-dependent DNA helicase 2 subunit 2
D MRX complex
D DNA Ligase 4 complex
D X-family DNA polymerases
E MNEG_0586 hypothetical protein K03512 POLL; DNA polymerase lambda [EC:2.7.7.7 4.2.99.-]
D Other NHEJ factors
E MNEG_4264 Transcription factor bHLH140 K10863 APTX; aprataxin [EC:3.6.1.70 3.6.1.71 3.6.1.72]
E MNEG_7541 Structural maintenance of chromosomes flexible hinge domain-containing protein 1 K23113 SMCHD1; structural maintenance of chromosomes flexible hinge domain-containing protein 1
C FA (Fanconi anemia) pathway
D FA core complex
E MNEG_3668 E3 ubiquitin-protein ligase FANCL K10606 FANCL; E3 ubiquitin-protein ligase FANCL [EC:2.3.2.27]
D FA core complex binding factors
E MNEG_6595 ubiquitin-conjugating enzyme E2T (putative) K13960 UBE2T; ubiquitin-conjugating enzyme E2 T [EC:2.3.2.23]
D Bloom's syndrome complex (BTR)
E MNEG_8196 topoisomerase (DNA) III beta K03165 TOP3; DNA topoisomerase III [EC:5.6.2.1]
D FANCD2-I complex
E MNEG_4586 Fanconi anemia group I protein K10895 FANCI; fanconi anemia group I protein
D Downstream FA components
E MNEG_12354 hypothetical protein K08775 BRCA2; breast cancer 2 susceptibility protein
E MNEG_3590 Fanconi anemia group J protein K15362 BRIP1; fanconi anemia group J protein [EC:5.6.2.3]
D Other FA pathway factors
E MNEG_7488 putative DNA repair protein RAD51 like protein K04482 RAD51; DNA repair protein RAD51
E MNEG_5735 RAD51-like protein 2 K10870 RAD51L2; RAD51-like protein 2
E MNEG_1572 hypothetical protein K15361 WDR48; WD repeat-containing protein 48
C Other DSBR factors
D Protein phosphatase 6
E MNEG_7676 hypothetical protein K15498 PPP6C; serine/threonine-protein phosphatase 6 catalytic subunit [EC:3.1.3.16]
D Ubiquitin ligases
D Others
E MNEG_13719 putative Gastrulation defective protein 1 like protein K24752 WDR70; WD repeat-containing protein 70
E MNEG_6085 hypothetical protein K24755 WDR76; WD repeat-containing protein 76
B TLS (translesion DNA synthesis) factors
C Y-family DNA polymerases
D MNEG_12147 hypothetical protein K03509 POLH; DNA polymerase eta [EC:2.7.7.7]
D MNEG_6991 DNA polymerase iota subunit K03510 POLI; DNA polymerase iota [EC:2.7.7.7]
D MNEG_15180 DNA polymerase kappa subunit K03511 POLK; DNA polymerase kappa [EC:2.7.7.7]
D MNEG_14421 DNA repair protein REV1 K03515 REV1; DNA repair protein REV1 [EC:2.7.7.-]
C B-family DNA polymerases
C A-family DNA polymerase
C Rad6 epistasis group
D MNEG_0735 hypothetical protein K10573 UBE2A; ubiquitin-conjugating enzyme E2 A [EC:2.3.2.23]
D MNEG_13079 ubc1, ubiquitin carrier protein 1 K10573 UBE2A; ubiquitin-conjugating enzyme E2 A [EC:2.3.2.23]
D MNEG_13566 Ubiquitin-conjugating enzyme E2 variant 1C K10704 UBE2V; ubiquitin-conjugating enzyme E2 variant
D MNEG_0004 ubiquitin-conjugating enzyme E2 N K10580 UBE2N; ubiquitin-conjugating enzyme E2 N [EC:2.3.2.23]
C Other TLS factors
D MNEG_1959 Proliferating cell nuclear antigen K04802 PCNA; proliferating cell nuclear antigen
D MNEG_8774 Proliferating cell nuclear antigen K04802 PCNA; proliferating cell nuclear antigen
B Check point factors
C Rad9-Hus1-Rad1 complex
D MNEG_11743 HUS1 checkpoint protein K10903 HUS1; HUS1 checkpoint protein
D MNEG_14307 hypothetical protein K02830 HRAD1; cell cycle checkpoint protein [EC:3.1.11.2]
C HRAD17(Rad24)-RFC complex
D MNEG_8127 replication factor C subunit 2/4 K10755 RFC2_4; replication factor C subunit 2/4
D MNEG_5877 Replication factor C subunit 5 K10756 RFC3_5; replication factor C subunit 3/5
D MNEG_4777 Replication factor C subunit 3 K10756 RFC3_5; replication factor C subunit 3/5
C Rad17-Mec3-Ddc1 complex
D MNEG_14307 hypothetical protein K02830 HRAD1; cell cycle checkpoint protein [EC:3.1.11.2]
C FPC (fork protection complex)
C Triple T complex
D MNEG_6569 hypothetical protein K20403 TTI1; TELO2-interacting protein 1
C BAG6-UBL4A-GET4 complex
D MNEG_1190 hypothetical protein K23387 GET4; golgi to ER traffic protein 4
C Other check point factors
D MNEG_6430 ataxia telangectasia mutated family protein K04728 ATM; serine-protein kinase ATM [EC:2.7.11.1]
D MNEG_5014 hypothetical protein K06630 YWHAE; 14-3-3 protein epsilon
D MNEG_7500 14-3-3-like protein K06630 YWHAE; 14-3-3 protein epsilon
D MNEG_5754 hypothetical protein K06632 WEE1; wee1-like protein kinase [EC:2.7.11.1]
D MNEG_0630 NIMA (never in mitosis a)-related kinase K08857 NEK1_4_5; serine/threonine-protein kinase Nek1/4/5 [EC:2.7.11.1]
D MNEG_3316 hypothetical protein K22422 DONSON; protein downstream neighbor of Son
D MNEG_10178 Vacuolar protein sorting-associating protein 4B K12196 VPS4; vacuolar protein-sorting-associated protein 4
D MNEG_6622 vacuolar protein-sorting-associated protein 4 K12196 VPS4; vacuolar protein-sorting-associated protein 4
B Other factors with a suspected DNA repair function
C DNA polymerases
D MNEG_3356 DNA polymerase theta subunit K02349 POLQ; DNA polymerase theta [EC:2.7.7.7]
D MNEG_4911 Coiled-coil domain-containing protein K22761 PRIMPOL; DNA-directed primase/polymerase protein [EC:2.7.7.102 2.7.7.7]
C Nucleases
D MNEG_0034 DNA cross-link repair 1A protein K15340 DCLRE1A; DNA cross-link repair 1A protein
D MNEG_7992 hypothetical protein K17815 EXO5; exonuclease V [EC:3.1.-.-]
C Helicases
D MNEG_0466 Putative DNA repair and recombination protein RAD26-like protein K20098 ERCC6L2; DNA excision repair protein ERCC-6-like 2 [EC:5.6.2.-]
C PSO4 complex
D MNEG_6235 pre-mRNA-processing factor 19 K10599 PRPF19; pre-mRNA-processing factor 19 [EC:2.3.2.27]
D MNEG_0137 pre-mRNA-splicing factor SPF27 K12861 BCAS2; pre-mRNA-splicing factor SPF27
D MNEG_12233 PP1/PP2A phosphatases pleiotropic regulator PRL1 K12862 PLRG1; pleiotropic regulator 1
C Modulation of nucleotide pools
D MNEG_1641 ADP-ribose pyrophosphatase K03574 mutT; 8-oxo-dGTP diphosphatase [EC:3.6.1.55]
D MNEG_14463 dUTP pyrophosphatase K01520 dut; dUTP diphosphatase [EC:3.6.1.23]
D MNEG_13580 ribonucleoside-diphosphate reductase largesubunit K10807 RRM1; ribonucleoside-diphosphate reductase subunit M1 [EC:1.17.4.1]
D MNEG_1584 ribonucleoside-diphosphate reductasesubunit M2 K10808 RRM2; ribonucleoside-diphosphate reductase subunit M2 [EC:1.17.4.1]
D MNEG_1585 ribonucleoside-diphosphate reductasesubunit M2 K10808 RRM2; ribonucleoside-diphosphate reductase subunit M2 [EC:1.17.4.1]
D MNEG_10972 ribonucleoside-diphosphate reductase subunit M2 K10808 RRM2; ribonucleoside-diphosphate reductase subunit M2 [EC:1.17.4.1]
D MNEG_10933 ribonucleoside-diphosphate reductase subunit M2 K10808 RRM2; ribonucleoside-diphosphate reductase subunit M2 [EC:1.17.4.1]
#
AProkaryotic type
B SSBR (single strand breaks repair)
C Direct repair
D MNEG_7529 methylated-DNA-protein-cysteine methyltransferase K00567 ogt; methylated-DNA-[protein]-cysteine S-methyltransferase [EC:2.1.1.63]
D MNEG_13885 deoxyribodipyrimidine photo-lyase K01669 phr; deoxyribodipyrimidine photo-lyase [EC:4.1.99.3]
C BER (base exicision repair)
D DNA glycosylases
E MNEG_14787 uracil-DNA glycosylase K03648 UNG; uracil-DNA glycosylase [EC:3.2.2.27]
E MNEG_0581 DNA-3-methyladenine glycosylase II K01247 alkA; DNA-3-methyladenine glycosylase II [EC:3.2.2.21]
E MNEG_6754 A/G-specific adenine glycosylase K03575 mutY; A/G-specific adenine glycosylase [EC:3.2.2.31]
E MNEG_9036 formamidopyrimidine-DNA glycosylase K10563 mutM; formamidopyrimidine-DNA glycosylase [EC:3.2.2.23 4.2.99.18]
E MNEG_5657 endonuclease III K10773 NTHL1; endonuclease III [EC:3.2.2.- 4.2.99.18]
D AP endonucleases
D RecJ
D DNA ligase
D DNA polymerase I
C NER (nucleotide excision repair)
D GGR (global genome repair) factors
D TCR (transcription coupled repair) factors
E DNA-directed RNA polymerase complex (RNAP)
E TRCF (transcription-repair coupling factor)
C MMR (mismatch excision repair)
D Mismatch and loop recognition factors
D Molecular matchmaker
D Strand discrimination factor
D DNA exonucleases
D DNA polymerase III holoenzyme
D DNA ligase
D Other MMR factors
B DSBR (double strand breaks repair)
C HR (homologous recombination)
D RecBC pathway proteins
D RecFOR pathway proteins
D AddAB pathway proteins
D Other HR factor
D Archaeal homologous recombinant proteins
C NHEJ (non-homologous end-joining)
D Two-component NHEJ DNA repair complex
D SHIIR (short-homology-independent illegitimate recombination)
E Facilitator
F MNEG_5564 DNA gyrase subunit B K02470 gyrB; DNA gyrase subunit B [EC:5.6.2.2]
E Supressor
D SHDIR (short-homology-dependent illegitimate recombination)
E RecET pathway
E Facilitator
E Supressor
B TLS (translesion DNA synthesis) factors
C Y-family DNA polymerases
C Other SOS response factors
B Other factors with a suspected DNA repair function
C DNA polymerase
C DNA helicases
C Modulation of nucleotide pools
D MNEG_1641 ADP-ribose pyrophosphatase K03574 mutT; 8-oxo-dGTP diphosphatase [EC:3.6.1.55]
D MNEG_14463 dUTP pyrophosphatase K01520 dut; dUTP diphosphatase [EC:3.6.1.23]
C Others
!
#
#[ BRITE | KEGG2 | KEGG ]
#Last updated: March 29, 2024