KEGG   PATHWAY: ath00908
Entry
ath00908                    Pathway                                
Name
Zeatin biosynthesis - Arabidopsis thaliana (thale cress)
Description
Zeatin is a member of the cytokinin family, a class of phytohormones involved in various processes of growth and development in plants. Most abundant cytokinins are adenine-type, where the N6 position of adenine is substituted with an isoprenoid, such as in zeatin, or an aromatic side chain, such as in kinetin. Zeatin can be synthesized in two different pathways: the tRNA pathway and the AMP pathway. In the tRNA pathway zeatin is a recycled product of isopentenylated tRNAs. In the AMP pathway zeatin is synthesized from an isopentenyl donor, dimethylallyl diphosphate (DMAPP), and AMP, ADP, or ATP by isopentenyltransferases. After synthesis cytokinins can be glucosylated.
Class
Metabolism; Metabolism of terpenoids and polyketides
Pathway map
ath00908  Zeatin biosynthesis
ath00908

Other DBs
GO: 0009691
Organism
Arabidopsis thaliana (thale cress) [GN:ath]
Gene
AT3G63110  IPT3; isopentenyltransferase 3 [KO:K10760] [EC:2.5.1.27 2.5.1.112]
AT4G24650  IPT4; isopentenyltransferase 4 [KO:K10760] [EC:2.5.1.27 2.5.1.112]
AT5G19040  IPT5; isopentenyltransferase 5 [KO:K10760] [EC:2.5.1.27 2.5.1.112]
AT3G23630  IPT7; isopentenyltransferase 7 [KO:K10760] [EC:2.5.1.27 2.5.1.112]
AT1G25410  IPT6; isopentenyltransferase 6 [KO:K10760] [EC:2.5.1.27 2.5.1.112]
AT3G19160  IPT8; ATP/ADP isopentenyltransferase [KO:K10760] [EC:2.5.1.27 2.5.1.112]
AT1G68460  IPT1; isopentenyltransferase 1 [KO:K10760] [EC:2.5.1.27 2.5.1.112]
AT1G67110  CYP735A2; cytochrome P450, family 735, subfamily A, polypeptide 2 [KO:K10717]
AT5G38450  CYP735A1; cytochrome P450, family 735, subfamily A, polypeptide 1 [KO:K10717]
AT5G21482  CKX7; cytokinin oxidase 7 [KO:K00279] [EC:1.5.99.12]
AT4G29740  CKX4; cytokinin oxidase 4 [KO:K00279] [EC:1.5.99.12]
AT2G19500  CKX2; cytokinin oxidase 2 [KO:K00279] [EC:1.5.99.12]
AT5G56970  CKX3; cytokinin oxidase 3 [KO:K00279] [EC:1.5.99.12]
AT2G41510  CKX1; cytokinin oxidase/dehydrogenase 1 [KO:K00279] [EC:1.5.99.12]
AT1G75450  CKX5; cytokinin oxidase 5 [KO:K00279] [EC:1.5.99.12]
AT3G63440  CKX6; cytokinin oxidase/dehydrogenase 6 [KO:K00279] [EC:1.5.99.12]
AT5G05860  UGT76C2; UDP-glucosyl transferase 76C2 [KO:K13493] [EC:2.4.1.-]
AT5G05870  UGT76C1; UDP-glucosyl transferase 76C1 [KO:K13493] [EC:2.4.1.-]
AT2G36750  UGT73C1; UDP-glucosyl transferase 73C1 [KO:K13496] [EC:2.4.1.-]
AT3G53160  UGT73C7; UDP-glucosyl transferase 73C7 [KO:K13496] [EC:2.4.1.-]
AT2G36800  DOGT1; don-glucosyltransferase 1 [KO:K13496] [EC:2.4.1.-]
AT2G36770  UDP-Glycosyltransferase superfamily protein [KO:K13496] [EC:2.4.1.-]
AT2G36780  UDP-Glycosyltransferase superfamily protein [KO:K13496] [EC:2.4.1.-]
AT2G36760  UGT73C2; UDP-glucosyl transferase 73C2 [KO:K13496] [EC:2.4.1.-]
AT1G22400  UGT85A1; UDP-Glycosyltransferase superfamily protein [KO:K23452] [EC:2.4.1.-]
AT1G22380  UGT85A3; UDP-glucosyl transferase 85A3 [KO:K23452] [EC:2.4.1.-]
AT1G22340  UGT85A7; UDP-glucosyl transferase 85A7 [KO:K23452] [EC:2.4.1.-]
AT1G78270  UGT85A4; UDP-glucosyl transferase 85A4 [KO:K23452] [EC:2.4.1.-]
AT1G22360  UGT85A2; UDP-glucosyl transferase 85A2 [KO:K23452] [EC:2.4.1.-]
AT1G22370  UGT85A5; UDP-glucosyl transferase 85A5 [KO:K23452] [EC:2.4.1.-]
AT2G27760  IPT2; tRNAisopentenyltransferase 2 [KO:K00791] [EC:2.5.1.75]
AT5G20040  IPT9; isopentenyltransferase 9 [KO:K00791] [EC:2.5.1.75]
Compound
C00002  ATP
C00008  ADP
C00015  UDP
C00019  S-Adenosyl-L-methionine
C00020  AMP
C00029  UDP-glucose
C00033  Acetate
C00147  Adenine
C00170  5'-Methylthioadenosine
C00190  UDP-D-xylose
C00235  Dimethylallyl diphosphate
C00371  Zeatin
C00979  O-Acetyl-L-serine
C01513  Lupinate
C01804  Discadenine
C02029  Dihydrozeatin
C03300  O-beta-D-Xylosylzeatin
C03423  O-beta-D-Glucosylzeatin
C04083  N6-(Delta2-Isopentenyl)-adenine
C04432  tRNA containing 6-isopentenyladenosine
C04713  N6-(delta2-Isopentenyl)-adenosine 5'-monophosphate
C07330  3-Methyl-2-butenal
C15545  cis-Zeatin
C15546  O-beta-D-Glucopyranosyl-cis-zeatin
C16424  Isopentenyladenosine-5'-triphosphate
C16426  Isopentenyladenosine-5'-diphosphate
C16427  Isopentenyl adenosine
C16428  trans-Zeatin riboside triphosphate
C16429  trans-Zeatin riboside diphosphate
C16430  trans-Zeatin riboside monophosphate
C16431  trans-Zeatin riboside
C16441  cis-Zeatin riboside monophosphate
C16443  trans-Zeatin-7-beta-D-glucoside
C16445  Dihydrozeatin riboside monophosphate
C16447  Dihydrozeatin riboside
C16448  Dihydrozeatin-O-glucoside
C16449  cis-Zeatin riboside
C16467  cis-Prenyl-tRNA
C17324  tRNA adenine
Reference
  Authors
Kasahara H, Takei K, Ueda N, Hishiyama S, Yamaya T, Kamiya Y, Yamaguchi S, Sakakibara H.
  Title
Distinct isoprenoid origins of cis- and trans-zeatin biosyntheses in Arabidopsis.
  Journal
J Biol Chem 279:14049-54 (2004)
DOI:10.1074/jbc.M314195200
Reference
  Authors
Takei K, Sakakibara H, Sugiyama T.
  Title
Identification of genes encoding adenylate isopentenyltransferase, a cytokinin biosynthesis enzyme, in Arabidopsis thaliana.
  Journal
J Biol Chem 276:26405-10 (2001)
DOI:10.1074/jbc.M102130200
Reference
  Authors
Kakimoto T.
  Title
Identification of plant cytokinin biosynthetic enzymes as dimethylallyl diphosphate:ATP/ADP isopentenyltransferases.
  Journal
Plant Cell Physiol 42:677-85 (2001)
DOI:10.1093/pcp/pce112
Reference
  Authors
Takei K, Yamaya T, Sakakibara H.
  Title
Arabidopsis CYP735A1 and CYP735A2 encode cytokinin hydroxylases that catalyze the biosynthesis of trans-Zeatin.
  Journal
J Biol Chem 279:41866-72 (2004)
DOI:10.1074/jbc.M406337200
Reference
  Authors
Hou B, Lim EK, Higgins GS, Bowles DJ.
  Title
N-glucosylation of cytokinins by glycosyltransferases of Arabidopsis thaliana.
  Journal
J Biol Chem 279:47822-32 (2004)
DOI:10.1074/jbc.M409569200
Reference
PMID:9874810
  Authors
Martin RC, Mok MC, Mok DW
  Title
Isolation of a cytokinin gene, ZOG1, encoding zeatin O-glucosyltransferase from Phaseolus lunatus.
  Journal
Proc Natl Acad Sci U S A 96:284-9 (1999)
DOI:10.1073/pnas.96.1.284
Reference
  Authors
Martin RC, Mok MC, Mok DW
  Title
A gene encoding the cytokinin enzyme zeatin O-xylosyltransferase of Phaseolus vulgaris.
  Journal
Plant Physiol 120:553-8 (1999)
DOI:10.1104/pp.120.2.553
Reference
  Authors
Veach YK, Martin RC, Mok DW, Malbeck J, Vankova R, Mok MC
  Title
O-glucosylation of cis-zeatin in maize. Characterization of genes, enzymes, and endogenous cytokinins.
  Journal
Plant Physiol 131:1374-80 (2003)
DOI:10.1104/pp.017210
Reference
  Authors
Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmulling T
  Title
Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity.
  Journal
Plant Cell 15:2532-50 (2003)
DOI:10.1105/tpc.014928
Reference
  Authors
Miyawaki K, Tarkowski P, Matsumoto-Kitano M, Kato T, Sato S, Tarkowska D, Tabata S, Sandberg G, Kakimoto T
  Title
Roles of Arabidopsis ATP/ADP isopentenyltransferases and tRNA isopentenyltransferases in cytokinin biosynthesis.
  Journal
Proc Natl Acad Sci U S A 103:16598-603 (2006)
DOI:10.1073/pnas.0603522103
Related
pathway
ath00900  Terpenoid backbone biosynthesis
KO pathway
ko00908   
LinkDB

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