| Entry |
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| Name |
malonyl-[acyl-carrier protein] O-methyltransferase;
BioC
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| Class |
Transferases;
Transferring one-carbon groups;
Methyltransferases
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| Sysname |
S-adenosyl-L-methionine:malonyl-[acyl-carrier protein] O-methyltransferase
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| Reaction(IUBMB) |
S-adenosyl-L-methionine + malonyl-[acyl-carrier protein] = S-adenosyl-L-homocysteine + malonyl-[acyl-carrier protein] methyl ester [RN: R09543]
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| Reaction(KEGG) |
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| Substrate |
S-adenosyl-L-methionine [CPD: C00019];
malonyl-[acyl-carrier protein] [CPD: C01209]
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| Product |
S-adenosyl-L-homocysteine [CPD: C00021];
malonyl-[acyl-carrier protein] methyl ester [CPD: C19673]
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| Comment |
Involved in an early step of biotin biosynthesis in Gram-negative bacteria. This enzyme catalyses the transfer of a methyl group to the omega-carboxyl group of malonyl-[acyl-carrier protein] forming a methyl ester. The methyl ester is recognized by the fatty acid synthetic enzymes, which process it via the fatty acid elongation cycle to give pimelyl-[acyl-carrier-protein] methyl ester [5]. While the enzyme can also accept malonyl-CoA, it has a much higher activity with malonyl-[acyl-carrier protein] [6]
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| History |
EC 2.1.1.197 created 2010, modified 2013
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| Pathway |
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| Orthology |
| K02169 | malonyl-CoA O-methyltransferase |
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| Genes |
» show all
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| Reference |
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| Authors |
Del Campillo-Campbell A, Kayajanian G, Campbell A, Adhya S |
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| Title |
Biotin-requiring mutants of Escherichia coli K-12. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Rolfe B, Eisenberg MA |
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| Title |
Genetic and biochemical analysis of the biotin loci of Escherichia coli K-12. |
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| Journal |
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| Reference |
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| Authors |
Otsuka AJ, Buoncristiani MR, Howard PK, Flamm J, Johnson C, Yamamoto R, Uchida K, Cook C, Ruppert J, Matsuzaki J |
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| Title |
The Escherichia coli biotin biosynthetic enzyme sequences predicted from the nucleotide sequence of the bio operon. |
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| Journal |
J Biol Chem 263:19577-85 (1988) |
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| Sequence |
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| Reference |
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| Authors |
Cleary PP, Campbell A |
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| Title |
Deletion and complementation analysis of biotin gene cluster of Escherichia coli. |
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| Journal |
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| Reference |
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| Authors |
Lin S, Hanson RE, Cronan JE |
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| Title |
Biotin synthesis begins by hijacking the fatty acid synthetic pathway. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Lin S, Cronan JE |
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| Title |
The BioC O-methyltransferase catalyzes methyl esterification of malonyl-acyl carrier protein, an essential step in biotin synthesis. |
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| Journal |
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| Other DBs |
| ExPASy - ENZYME nomenclature database: | 2.1.1.197 |
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| LinkDB |
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