KEGG   ORTHOLOGY: K04835
Entry
K04835                      KO                                     
Symbol
mal
Name
methylaspartate ammonia-lyase [EC:4.3.1.2]
Pathway
map00630  Glyoxylate and dicarboxylate metabolism
map00660  C5-Branched dibasic acid metabolism
map01100  Metabolic pathways
map01120  Microbial metabolism in diverse environments
map01200  Carbon metabolism
Module
M00740  Methylaspartate cycle
Reaction
R03696  L-threo-3-methylaspartate ammonia-lyase
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09101 Carbohydrate metabolism
   00630 Glyoxylate and dicarboxylate metabolism
    K04835  mal; methylaspartate ammonia-lyase
   00660 C5-Branched dibasic acid metabolism
    K04835  mal; methylaspartate ammonia-lyase
Enzymes [BR:ko01000]
 4. Lyases
  4.3  Carbon-nitrogen lyases
   4.3.1  Ammonia-lyases
    4.3.1.2  methylaspartate ammonia-lyase
     K04835  mal; methylaspartate ammonia-lyase
Other DBs
COG: COG3799
GO: 0050096
Genes
AFM: AFUA_3G00790
NFI: NFIA_001410
AOR: AO090010000710
AFV: AFLA_014211
ACHE: ACHE_70014S
PDP: PDIP_33520
ECE: Z0892
ECS: ECs_0761
ECF: ECH74115_0829
ETW: ECSP_0779
ELX: CDCO157_0741
ECOO: ECRM13514_0750
ECOH: ECRM13516_0697
EOK: G2583_0892
ELR: ECO55CA74_04340
ECM: EcSMS35_0749
EUM: ECUMN_0814
ECT: ECIAI39_0701
EOC: CE10_0730
ELO: EC042_0753
EFE: EFER_2378
EMA: C1192_08695
ESZ: FEM44_18110
ESF: ACK8NJ_16265
SES: SARI_02201
SDY: SDY_0673
SDZ: Asd1617_00848
SHIG: LXH19_15265
ESH: C1N69_07900
EPU: QVH39_07900
EAX: AAHF05_07650
ENTE: GXP72_07970
KOX: KOX_14665
KOE: A225_1756
KOY: J415_22870
KOM: HR38_13440
KOK: KONIH1_08640
KOC: AB185_27495
KLL: BJF97_09010
KLW: DA718_19620
KGR: JJJ10_19550
KPAS: LUW96_15420
KLC: K7H21_19535
KLM: BWI76_08475
KLP: GUC22_18805
CKO: CKO_02419
CFD: CFNIH1_13770
CBRA: A6J81_09350
CWE: CO701_15165
CYO: CD187_16565
CPOT: FOB25_04015
CFQ: C2U38_07700
CSED: JY391_14750
CAMA: F384_03285
CAF: AL524_19000
CIF: AL515_08250
CFAR: CI104_08015
CIR: C2U53_17235
CIE: AN232_22705
CPAR: CUC49_03400
CTEL: GBC03_21985
CITZ: E4Z61_09960
CARS: E1B03_08815
CIX: M4I31_15970
CIB: HF677_016175
CENS: P2W74_15920
CITR: GUC46_16240
CIQ: FD428_14755
CIY: HAP28_08240
ESC: Entcl_1732
KLE: AO703_13285
BUF: D8682_20785
BFT: MNO13_05010
BSEL: RHD99_04810
YRE: HEC60_20895
PVJ: LMA04_06040
YEN: YE4043
YEP: YE105_C3763
YEY: Y11_31521
YEW: CH47_3688
YET: CH48_1495
YEF: FORC2_0151
YEE: YE5303_38771
YAL: AT01_1098
YIN: CH53_313
YRO: CH64_1340
YHI: D5F51_21360
YEL: LC20_00182
YCA: F0T03_21045
YAS: N0H69_09490
MMK: MU9_1023
HAV: AT03_08650
OPO: DSM2777_13860
PPUL: RO07_15990
BPE: BP0806 BP3177
BPC: BPTD_0804 BPTD_3139
BPER: BN118_0709
BPET: B1917_0643 B1917_2973
BPEU: Q425_31590 Q425_7420
BPAR: BN117_3385 BN117_3938
BPA: BPP3422 BPP3864
BBH: BN112_4085 BN112_4543
BBR: BB3872 BB4337
BBM: BN115_3544 BN115_4013
BBX: BBS798_3652 BBS798_4114
BFZ: BAU07_20575
BOH: AKI39_19475
BGM: CAL15_20690
XYK: GT347_12540
MLO: mlr6095
MLN: A9174_31760
MCI: Mesci_5690
MCIC: A4R28_21445
MOP: Mesop_6269
MAM: Mesau_05741
MAMO: A6B35_31635 A6B35_31710
MESW: A9K65_030880
MESM: EJ066_12585
MJR: EB229_32430
MERD: EB233_30065
MMEI: LRP31_10480
MESJ: MJ8_14450
MEWS: QAZ47_06665
MEWM: QAZ22_06695
RSUL: N2599_36735
BIC: LMTR13_26000
PPAN: ESD82_15275
GFU: KM031_18930
ALH: G6N82_05195
ELQ: Ga0102493_112996
MTAR: DF168_01552
LBV: JYG24_01975
TDE: TDE_2235
TPI: TREPR_1304
TPED: TPE_1176
TPK: JO40_01075
SCD: Spica_1659
PAH: Poras_1170
PCRE: NCTC12858_01580
PSOE: CE91St14_10350
PET: PEIBARAKI_4288
PROJ: KDN43_05785
TOH: BCB71_00035
PIT: PIN17_0467
PNI: J5A59_01360
DTH: DICTH_1229
DTU: Dtur_1344
CTC: CTC_02563
CTET: BN906_02814
CLJ: CLJU_c40610
CAH: CAETHG_1904
CSQ: CSCA_4328
CACE: CACET_c34410
CCK: Ccar_17555 Ccar_19615
CEU: A7L45_08020
CFM: BJL90_02575
CARG: RSJ17_03855
CDRK: B9W14_23400
CCOH: SAMEA4530647_2259
CLOS: DMR38_20135
CCAA: KQH81_15280
CTAG: LL095_20705
CLOO: RCG18_03800 RCG18_05855
HHW: NCTC503_02397
CALE: FDN13_04640
OCW: OW730_04540
PROQ: P6M73_02460
PETR: QKW49_11540
DSY: DSY3233
DHD: Dhaf_4400
DDH: Desde_3779
DMT: DESME_13810
DOR: Desor_5275
DAI: Desaci_4373
DMI: Desmer_4259
DCA: Desca_0604
DRM: Dred_2689
DRU: Desru_0927
DFG: B0537_13815
FWA: DCMF_09590
AACX: DEACI_0128
ACAE: HYG86_02375
CTHM: CFE_0619
FMS: M1R53_04075
PROM: QO263_09920
BYL: A4V09_10270
BHAN: CGC63_11295
BPRO: PMF13cell1_03217
BCOC: BLCOC_23090
BLAR: LC508_07905
VGU: HYG85_02135
VPY: HZI73_09950
EMJ: V1225_12890
EUU: ADJ67_05945
AMT: Amet_3414
TEM: JW646_17970
TGC: L0P85_10055
TMY: TEMA_08780
TPET: TPHSE_23060
PBQ: C3V37_01025
EAC: EAL2_808p05890
CHY: CHY_0484 CHY_0582
TPZ: Tph_c20250
TEP: TepRe1_0115
TAE: TepiRe1_0120
HOR: Hore_15430
HPK: Hprae_1850
HALS: D7D81_15595
IFN: GM661_12735
AAR: Acear_0480
HHL: Halha_1649
AFT: BBF96_08380
MFOM: VZL98_10615
TIS: P3962_02615
ECOU: M1R54_02150
PUF: UFO1_3073
PFT: JBW_01516
MANA: MAMMFC1_00632
STED: SPTER_10690
SSPH: SPSPH_031600
SOVA: SOV_11890
SSID: SPSIL_016820
SACV: SPACI_017830
SCAO: SCACP_11170
MANR: MPAN_001680
XCL: G4Z02_00740
TBK: HF295_06620
MBJ: KQ51_00879
TBM: BK010_00845
TBZ: BK011_00735
MSED: E3O41_00265
FRP: AX769_17710
NMES: H9L09_01760
MHAI: OHB01_26590
MMAR: MODMU_4014
MOM: KUM42_03575
KAL: KALB_5717
MTUA: CSH63_31865
BSOL: FSW04_00285
BALA: DSM104299_03890
ABAO: BEQ56_05600
CPOR: BED41_14050
APRP: KIH16_11020
FVA: FV113G1_25160
FUL: C4N20_01145
FMO: C4N19_13190
FHO: H9Q81_10155
IPO: Ilyop_0556
CSOM: MKD34_06450
HAL: VNG_2289G(mal)
HSL: OE_4207F(mal)
HANR: LJ422_09240
HAGS: JT689_10590
HABO: JRZ79_08965
HALX: M0R89_18080
HAXY: NGM07_04610
SALI: L593_04670
HMA: rrnAC0687(mal)
HSIN: KDQ40_06775
HHI: HAH_1339(mal)
HHN: HISP_06855
HAB: SG26_14700
HTA: BVU17_06525
HALJ: G9465_08575
HMU: Hmuk_2982
HALZ: E5139_02185
HALL: LC1Hm_2319(mal)
HAZP: GBQ70_02185
HRR: HZS55_19995
HPEL: HZS54_23495
HGI: ABY42_16395
HTU: Htur_1592
HSAL: JMJ58_00675
HAKZ: J0X25_14770
NMG: Nmag_3336(mal)
HXA: Halxa_3157
NAT: NJ7G_3291
NPE: Natpe_2592
NVR: FEJ81_11890
NPL: FGF80_10930
NAY: HYG81_14700
HALY: HYG82_17320
HJT: DVR14_02945
HLO: J0X27_02305
NGE: Natgr_2182 Natgr_3386
HLC: CHINAEXTREME16085
HRU: Halru_1550
SAWL: NGM29_08780
HALV: NGM15_00945
NOU: Natoc_1511
HLR: HALLA_00800 HALLA_10845
NAJ: B1756_12015
NAG: AArcMg_1647
NAN: AArc1_1992
NARA: QQ977_05690
NBG: DV706_12990
NAS: GCU68_13735
HDA: BB347_04020
 » show all
Reference
PMID:21252347
  Authors
Khomyakova M, Bukmez O, Thomas LK, Erb TJ, Berg IA
  Title
A methylaspartate cycle in haloarchaea.
  Journal
Science 331:334-7 (2011)
DOI:10.1126/science.1196544
  Sequence
[hma:rrnAC0687]
Reference
PMID:22005738
  Authors
Raj H, Puthan Veetil V, Szymanski W, Dekker FJ, Quax WJ, Feringa BL, Janssen DB, Poelarends GJ.
  Title
Characterization of a thermostable methylaspartate ammonia lyase from Carboxydothermus hydrogenoformans.
  Journal
Appl Microbiol Biotechnol 94:385-97 (2012)
DOI:10.1007/s00253-011-3615-6
  Sequence
[chy:CHY_0484]
LinkDB

KEGG   ENZYME: 4.3.1.2
Entry
EC 4.3.1.2                  Enzyme                                 
Name
methylaspartate ammonia-lyase;
beta-methylaspartase;
3-methylaspartase;
L-threo-3-methylaspartate ammonia-lyase
Class
Lyases;
Carbon-nitrogen lyases;
Ammonia-lyases
Sysname
L-threo-3-methylaspartate ammonia-lyase (mesaconate-forming)
Reaction(IUBMB)
L-threo-3-methylaspartate = mesaconate + NH3 [RN:R03696]
Reaction(KEGG)
R03696
Substrate
L-threo-3-methylaspartate [CPD:C03618]
Product
mesaconate [CPD:C01732];
NH3 [CPD:C00014]
Comment
A cobalamin protein.
History
EC 4.3.1.2 created 1961
Pathway
ec00630  Glyoxylate and dicarboxylate metabolism
ec00660  C5-Branched dibasic acid metabolism
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K04835  methylaspartate ammonia-lyase
Genes
AFM: AFUA_3G00790
NFI: NFIA_001410
AOR: AO090010000710
AFV: AFLA_014211
ACHE: ACHE_70014S
PDP: PDIP_33520
ECE: Z0892
ECS: ECs_0761
ECF: ECH74115_0829
ETW: ECSP_0779
ELX: CDCO157_0741
ECOO: ECRM13514_0750
ECOH: ECRM13516_0697
EOK: G2583_0892
ELR: ECO55CA74_04340
ECM: EcSMS35_0749
EUM: ECUMN_0814
ECT: ECIAI39_0701
EOC: CE10_0730
ELO: EC042_0753
EFE: EFER_2378
EMA: C1192_08695
ESZ: FEM44_18110
ESF: ACK8NJ_16265
SES: SARI_02201
SDY: SDY_0673
SDZ: Asd1617_00848
SHIG: LXH19_15265
ESH: C1N69_07900
EPU: QVH39_07900
EAX: AAHF05_07650
ENTE: GXP72_07970
KOX: KOX_14665
KOE: A225_1756
KOY: J415_22870
KOM: HR38_13440
KOK: KONIH1_08640
KOC: AB185_27495
KLL: BJF97_09010
KLW: DA718_19620
KGR: JJJ10_19550
KPAS: LUW96_15420
KLC: K7H21_19535
KLM: BWI76_08475
KLP: GUC22_18805
CKO: CKO_02419
CFD: CFNIH1_13770
CBRA: A6J81_09350
CWE: CO701_15165
CYO: CD187_16565
CPOT: FOB25_04015
CFQ: C2U38_07700
CSED: JY391_14750
CAMA: F384_03285
CAF: AL524_19000
CIF: AL515_08250
CFAR: CI104_08015
CIR: C2U53_17235
CIE: AN232_22705
CPAR: CUC49_03400
CTEL: GBC03_21985
CITZ: E4Z61_09960
CARS: E1B03_08815
CIX: M4I31_15970
CIB: HF677_016175
CENS: P2W74_15920
CITR: GUC46_16240
CIQ: FD428_14755
CIY: HAP28_08240
ESC: Entcl_1732
KLE: AO703_13285
BUF: D8682_20785
BFT: MNO13_05010
BSEL: RHD99_04810
YRE: HEC60_20895
PVJ: LMA04_06040
YEN: YE4043
YEP: YE105_C3763
YEY: Y11_31521
YEW: CH47_3688
YET: CH48_1495
YEF: FORC2_0151
YEE: YE5303_38771
YAL: AT01_1098
YIN: CH53_313
YRO: CH64_1340
YHI: D5F51_21360
YEL: LC20_00182
YCA: F0T03_21045
YAS: N0H69_09490
MMK: MU9_1023
HAV: AT03_08650
OPO: DSM2777_13860
PPUL: RO07_15990
BPE: BP0806 BP3177
BPC: BPTD_0804 BPTD_3139
BPER: BN118_0709
BPET: B1917_0643 B1917_2973
BPEU: Q425_31590 Q425_7420
BPAR: BN117_3385 BN117_3938
BPA: BPP3422 BPP3864
BBH: BN112_4085 BN112_4543
BBR: BB3872 BB4337
BBM: BN115_3544 BN115_4013
BBX: BBS798_3652 BBS798_4114
BFZ: BAU07_20575
BOH: AKI39_19475
BGM: CAL15_20690
XYK: GT347_12540
MLO: mlr6095
MLN: A9174_31760
MCI: Mesci_5690
MCIC: A4R28_21445
MOP: Mesop_6269
MAM: Mesau_05741
MAMO: A6B35_31635 A6B35_31710
MESW: A9K65_030880
MESM: EJ066_12585
MJR: EB229_32430
MERD: EB233_30065
MMEI: LRP31_10480
MESJ: MJ8_14450
MEWS: QAZ47_06665
MEWM: QAZ22_06695
RSUL: N2599_36735
BIC: LMTR13_26000
PPAN: ESD82_15275
GFU: KM031_18930
ALH: G6N82_05195
ELQ: Ga0102493_112996
MTAR: DF168_01552
LBV: JYG24_01975
TDE: TDE_2235
TPI: TREPR_1304
TPED: TPE_1176
TPK: JO40_01075
SCD: Spica_1659
PAH: Poras_1170
PCRE: NCTC12858_01580
PSOE: CE91St14_10350
PET: PEIBARAKI_4288
PROJ: KDN43_05785
TOH: BCB71_00035
PIT: PIN17_0467
PNI: J5A59_01360
DTH: DICTH_1229
DTU: Dtur_1344
CTC: CTC_02563
CTET: BN906_02814
CLJ: CLJU_c40610
CAH: CAETHG_1904
CSQ: CSCA_4328
CACE: CACET_c34410
CCK: Ccar_17555 Ccar_19615
CEU: A7L45_08020
CFM: BJL90_02575
CARG: RSJ17_03855
CDRK: B9W14_23400
CCOH: SAMEA4530647_2259
CLOS: DMR38_20135
CCAA: KQH81_15280
CTAG: LL095_20705
CLOO: RCG18_03800 RCG18_05855
HHW: NCTC503_02397
CALE: FDN13_04640
OCW: OW730_04540
PROQ: P6M73_02460
PETR: QKW49_11540
DSY: DSY3233
DHD: Dhaf_4400
DDH: Desde_3779
DMT: DESME_13810
DOR: Desor_5275
DAI: Desaci_4373
DMI: Desmer_4259
DCA: Desca_0604
DRM: Dred_2689
DRU: Desru_0927
DFG: B0537_13815
FWA: DCMF_09590
AACX: DEACI_0128
ACAE: HYG86_02375
CTHM: CFE_0619
FMS: M1R53_04075
PROM: QO263_09920
BYL: A4V09_10270
BHAN: CGC63_11295
BPRO: PMF13cell1_03217
BCOC: BLCOC_23090
BLAR: LC508_07905
VGU: HYG85_02135
VPY: HZI73_09950
EMJ: V1225_12890
EUU: ADJ67_05945
AMT: Amet_3414
TEM: JW646_17970
TGC: L0P85_10055
TMY: TEMA_08780
TPET: TPHSE_23060
PBQ: C3V37_01025
EAC: EAL2_808p05890
CHY: CHY_0484 CHY_0582
TPZ: Tph_c20250
TEP: TepRe1_0115
TAE: TepiRe1_0120
HOR: Hore_15430
HPK: Hprae_1850
HALS: D7D81_15595
IFN: GM661_12735
AAR: Acear_0480
HHL: Halha_1649
AFT: BBF96_08380
MFOM: VZL98_10615
TIS: P3962_02615
ECOU: M1R54_02150
PUF: UFO1_3073
PFT: JBW_01516
MANA: MAMMFC1_00632
STED: SPTER_10690
SSPH: SPSPH_031600
SOVA: SOV_11890
SSID: SPSIL_016820
SACV: SPACI_017830
SCAO: SCACP_11170
MANR: MPAN_001680
XCL: G4Z02_00740
TBK: HF295_06620
MBJ: KQ51_00879
TBM: BK010_00845
TBZ: BK011_00735
MSED: E3O41_00265
FRP: AX769_17710
NMES: H9L09_01760
MHAI: OHB01_26590
MMAR: MODMU_4014
MOM: KUM42_03575
KAL: KALB_5717
MTUA: CSH63_31865
BSOL: FSW04_00285
BALA: DSM104299_03890
ABAO: BEQ56_05600
CPOR: BED41_14050
APRP: KIH16_11020
FVA: FV113G1_25160
FUL: C4N20_01145
FMO: C4N19_13190
FHO: H9Q81_10155
IPO: Ilyop_0556
CSOM: MKD34_06450
HAL: VNG_2289G(mal)
HSL: OE_4207F(mal)
HANR: LJ422_09240
HAGS: JT689_10590
HABO: JRZ79_08965
HALX: M0R89_18080
HAXY: NGM07_04610
SALI: L593_04670
HMA: rrnAC0687(mal)
HSIN: KDQ40_06775
HHI: HAH_1339(mal)
HHN: HISP_06855
HAB: SG26_14700
HTA: BVU17_06525
HALJ: G9465_08575
HMU: Hmuk_2982
HALZ: E5139_02185
HALL: LC1Hm_2319(mal)
HAZP: GBQ70_02185
HRR: HZS55_19995
HPEL: HZS54_23495
HGI: ABY42_16395
HTU: Htur_1592
HSAL: JMJ58_00675
HAKZ: J0X25_14770
NMG: Nmag_3336(mal)
HXA: Halxa_3157
NAT: NJ7G_3291
NPE: Natpe_2592
NVR: FEJ81_11890
NPL: FGF80_10930
NAY: HYG81_14700
HALY: HYG82_17320
HJT: DVR14_02945
HLO: J0X27_02305
NGE: Natgr_2182 Natgr_3386
HLC: CHINAEXTREME16085
HRU: Halru_1550
SAWL: NGM29_08780
HALV: NGM15_00945
NOU: Natoc_1511
HLR: HALLA_00800 HALLA_10845
NAJ: B1756_12015
NAG: AArcMg_1647
NAN: AArc1_1992
NARA: QQ977_05690
NBG: DV706_12990
NAS: GCU68_13735
HDA: BB347_04020
 » show all
Reference
1  [PMID:13618029]
  Authors
BARKER HA, SMYTH RD, WAWSZKIEWICZ EJ, LEE MN, WILSON RM.
  Title
Enzymic preparation and characterization of an alpha-L-beta-methylaspartic acid.
  Journal
Arch Biochem Biophys 78:468-76 (1958)
DOI:10.1016/0003-9861(58)90371-0
Reference
2
  Authors
Bright HJ, Ingraham LL.
  Title
The preparation of crystalline beta-methylaspartase.
  Journal
Biochim Biophys Acta 44:586-588 (1960)
Other DBs
ExplorEnz - The Enzyme Database: 4.3.1.2
IUBMB Enzyme Nomenclature: 4.3.1.2
ExPASy - ENZYME nomenclature database: 4.3.1.2
BRENDA, the Enzyme Database: 4.3.1.2
CAS: 9033-26-5
LinkDB

KEGG   REACTION: R03696
Entry
R03696                      Reaction                               
Name
L-threo-3-methylaspartate ammonia-lyase
Definition
L-threo-3-Methylaspartate <=> Mesaconate + Ammonia
Equation
C03618 <=> C01732 + C00014
Reaction class
RC00979  C01732_C03618
Enzyme
4.3.1.2
Pathway
rn00630  Glyoxylate and dicarboxylate metabolism
rn00660  C5-Branched dibasic acid metabolism
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
rn01200  Carbon metabolism
Module
M00740  Methylaspartate cycle
Brite
Enzymatic reactions [BR:br08201]
 4. Lyase reactions
  4.3  Carbon-nitrogen lyases
   4.3.1  Ammonia-lyases
    4.3.1.2
     R03696  L-threo-3-Methylaspartate <=> Mesaconate + Ammonia
Orthology
K04835  methylaspartate ammonia-lyase [EC:4.3.1.2]
Other DBs
RHEA: 12832
LinkDB

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