KEGG   ORTHOLOGY: K10438
Entry
K10438                      KO                                     

Name
PHACB, CYP504B1
Definition
3-hydroxyphenylacetate 6-hydroxylase [EC:1.14.13.63]
Pathway
ko00643  Styrene degradation
ko01120  Microbial metabolism in diverse environments
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00643 Styrene degradation
    K10438  PHACB, CYP504B1; 3-hydroxyphenylacetate 6-hydroxylase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   00199 Cytochrome P450
    K10438  PHACB, CYP504B1; 3-hydroxyphenylacetate 6-hydroxylase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.13  With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.13.63  3-hydroxyphenylacetate 6-hydroxylase
     K10438  PHACB, CYP504B1; 3-hydroxyphenylacetate 6-hydroxylase
Cytochrome P450 [BR:ko00199]
 Cytochrome P450, fungi type
  CYP504 family
   K10438  PHACB, CYP504B1; 3-hydroxyphenylacetate 6-hydroxylase
Other DBs
RN: R02517
COG: COG2124
GO: 0047094
Genes
SLB: AWJ20_2761 AWJ20_5196(ERG5)
MGR: MGG_03352
FGR: FGSG_06447
FPU: FPSE_10099
FVR: FVEG_04417
FOX: FOXG_07489 FOXG_10783
NHE: NECHADRAFT_53600 NECHADRAFT_87111
MAW: MAC_02148
MAJ: MAA_00010 MAA_04437 MAA_06936
CMT: CCM_02612 CCM_07933
ANI: AN1397.2
ANG: ANI_1_12074(An08g00180)
CIM: CIMG_13120(CIMG07204)
PBL: PAAG_11576(PAAG_02928)
ABE: ARB_02605
PTE: PTT_06974
 » show all
Reference
  Authors
Ferrer-Sevillano F, Fernandez-Canon JM.
  Title
Novel phacB-encoded cytochrome P450 monooxygenase from Aspergillus nidulans with 3-hydroxyphenylacetate 6-hydroxylase and 3,4-dihydroxyphenylacetate 6-hydroxylase activities.
  Journal
Eukaryot Cell 6:514-20 (2007)
DOI:10.1128/EC.00226-06
  Sequence
[ani:AN1397.2]
LinkDB

KEGG   ENZYME: 1.14.13.63
Entry
EC 1.14.13.63               Enzyme                                 

Name
3-hydroxyphenylacetate 6-hydroxylase;
3-hydroxyphenylacetate 6-monooxygenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
3-hydroxyphenylacetate,NAD(P)H:oxygen oxidoreductase (6-hydroxylating)
Reaction(IUBMB)
3-hydroxyphenylacetate + NAD(P)H + H+ + O2 = 2,5-dihydroxyphenylacetate + NAD(P)+ + H2O [RN:R02515 R02517]
Reaction(KEGG)
R02515 R02517
Substrate
3-hydroxyphenylacetate [CPD:C05593];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
2,5-dihydroxyphenylacetate [CPD:C00544];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
3-hydroxyphenylacetate 6-hydroxylase from Flavobacterium sp. is highly specific for 3-hydroxyphenylacetate and uses NADH and NADPH as electron donors with similar efficiency.
History
EC 1.14.13.63 created 1999
Pathway
ec00643  Styrene degradation
ec01120  Microbial metabolism in diverse environments
Orthology
K10438  3-hydroxyphenylacetate 6-hydroxylase
Genes
SLB: AWJ20_2761 AWJ20_5196(ERG5)
MGR: MGG_03352
FGR: FGSG_06447
FPU: FPSE_10099
FVR: FVEG_04417
FOX: FOXG_07489 FOXG_10783
NHE: NECHADRAFT_53600 NECHADRAFT_87111
MAW: MAC_02148
MAJ: MAA_00010 MAA_04437 MAA_06936
CMT: CCM_02612 CCM_07933
ANI: AN1397.2
ANG: ANI_1_12074(An08g00180)
CIM: CIMG_13120(CIMG07204)
PBL: PAAG_11576(PAAG_02928)
ABE: ARB_02605
PTE: PTT_06974
 » show all
Reference
1  [PMID:1935954]
  Authors
Van Berkel WJ, Van Den Tweel WJ.
  Title
Purification and characterisation of 3-hydroxyphenylacetate 6-hydroxylase: a novel FAD-dependent monooxygenase from a Flavobacterium species.
  Journal
Eur J Biochem 201:585-92 (1991)
DOI:10.1111/j.1432-1033.1991.tb16318.x
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.63
IUBMB Enzyme Nomenclature: 1.14.13.63
ExPASy - ENZYME nomenclature database: 1.14.13.63
BRENDA, the Enzyme Database: 1.14.13.63
CAS: 114705-01-0
LinkDB

KEGG   REACTION: R02517
Entry
R02517                      Reaction                               

Name
3-hydroxyphenylacetate,NADPH:oxygen oxidoreductase (6-hydroxylating)
Definition
3-Hydroxyphenylacetate + Oxygen + NADPH + H+ <=> Homogentisate + NADP+ + H2O
Equation
Comment
NADH (see R02515)
Reaction class
RC00001  C00005_C00006
RC00046  C00544_C05593
Enzyme
Pathway
rn00643  Styrene degradation
rn01120  Microbial metabolism in diverse environments
Orthology
K10438  3-hydroxyphenylacetate 6-hydroxylase [EC:1.14.13.63]
Other DBs
RHEA: 22207
LinkDB

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