KEGG Orthology (KO) [BR:ko00001]
09100 Metabolism
09110 Biosynthesis of other secondary metabolites
00311 Penicillin and cephalosporin biosynthesis
K12745 cefF; deacetoxycephalosporin-C hydroxylase
09180 Brite Hierarchies
09181 Protein families: metabolism
01008 Polyketide biosynthesis proteins
K12745 cefF; deacetoxycephalosporin-C hydroxylase
Enzymes [BR:ko01000]
1. Oxidoreductases
1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
1.14.11.26 deacetoxycephalosporin-C hydroxylase
K12745 cefF; deacetoxycephalosporin-C hydroxylase
Polyketide biosynthesis proteins [BR:ko01008]
Nonribosomal peptide tailoring proteins
Others
K12745 cefF; deacetoxycephalosporin-C hydroxylase
deacetoxycephalosporin-C hydroxylase;
deacetylcephalosporin C synthase;
3'-methylcephem hydroxylase;
DACS;
DAOC hydroxylase;
deacetoxycephalosporin C hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
deacetoxycephalosporin C [CPD:C06565];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007]
Product
deacetylcephalosporin C [CPD:C03112];
succinate [CPD:C00042];
CO2 [CPD:C00011]
Comment
Requires iron(II). The enzyme can also use 3-exomethylenecephalosporin C as a substrate to form deacetoxycephalosporin C, although more slowly [2]. In Acremonium chrysogenum, the enzyme forms part of a bifunctional protein along with EC 1.14.20.1, deactoxycephalosporin-C synthase. It is a separate enzyme in Streptomyces clavuligerus.
Cephamycin C biosynthesis, aminoadipate + cycteine + valine => cephamycin C
Brite
Enzymatic reactions [BR:br08201]
1. Oxidoreductase reactions
1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
1.14.11.26
R05229 Deacetylcephalosporin C + Succinate + CO2 <=> Deacetoxycephalosporin C + 2-Oxoglutarate + Oxygen