ENZYME: 1.1.1.297
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Entry
EC 1.1.1.297 Enzyme
Name
limonene-1,2-diol dehydrogenase;
NAD+-dependent limonene-1,2-diol dehydrogenase
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
menth-8-ene-1,2-diol:NAD+ oxidoreductase
Reaction(IUBMB)
menth-8-ene-1,2-diol + NAD+ = 1-hydroxymenth-8-en-2-one + NADH + H+ (general reaction) [RN:
R08919
];
(1) (1S,2S,4R)-menth-8-ene-1,2-diol + NAD+ = (1S,4R)-1-hydroxymenth-8-en-2-one + NADH + H+ [RN:
R06399
];
(2) (1R,2R,4S)-menth-8-ene-1,2-diol + NAD+ = (1R,4S)-1-hydroxymenth-8-en-2-one + NADH + H+ [RN:
R09388
]
Reaction(KEGG)
R06399
R08919
R09388
Reaction
Substrate
menth-8-ene-1,2-diol [CPD:
C18020
];
NAD+ [CPD:
C00003
];
(1S,2S,4R)-menth-8-ene-1,2-diol [CPD:
C07276
];
(1R,2R,4S)-menth-8-ene-1,2-diol [CPD:
C19082
]
Product
1-hydroxymenth-8-en-2-one [CPD:
C18019
];
NADH [CPD:
C00004
];
H+ [CPD:
C00080
];
(1S,4R)-1-hydroxymenth-8-en-2-one [CPD:
C11937
];
(1R,4S)-1-hydroxymenth-8-en-2-one
Comment
While the enzyme from the Gram-positive bacterium Rhodococcus erythropolis DCL14 can use both (1S,2S,4R)- and (1R,2R,4S)-menth-8-ene-1,2-diol as substrate, activity is higher with (1S,2S,4R)-menth-8-ene-1,2-diol as substrate.
History
EC 1.1.1.297 created 2008
Pathway
ec00903
Limonene and pinene degradation
Reference
1 [PMID:
10224006
]
Authors
van der Werf MJ, Swarts HJ, de Bont JA
Title
Rhodococcus erythropolis DCL14 contains a novel degradation pathway for limonene.
Journal
Appl Environ Microbiol 65:2092-102 (1999)
DOI:
10.1128/AEM.65.5.2092-2102.1999
Other DBs
ExplorEnz - The Enzyme Database:
1.1.1.297
IUBMB Enzyme Nomenclature:
1.1.1.297
ExPASy - ENZYME nomenclature database:
1.1.1.297
BRENDA, the Enzyme Database:
1.1.1.297
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