KEGG   ENZYME: 1.1.1.329
Entry
EC 1.1.1.329                Enzyme                                 

Name
2-deoxy-scyllo-inosamine dehydrogenase;
neoA (gene name);
kanK (gene name, ambiguous);
kanE (gene name, ambiguous)
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
Sysname
2-deoxy-scyllo-inosamine:NAD(P)+ 1-oxidoreductase
Reaction(IUBMB)
2-deoxy-scyllo-inosamine + NAD(P)+ = 3-amino-2,3-dideoxy-scyllo-inosose + NAD(P)H + H+ [RN:R08892 R10130]
Reaction(KEGG)
R08892 R10130
Substrate
2-deoxy-scyllo-inosamine [CPD:C17580];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006]
Product
3-amino-2,3-dideoxy-scyllo-inosose [CPD:C17581];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
Requires zinc. Involved in the biosynthetic pathways of several clinically important aminocyclitol antibiotics, including kanamycin, neomycin and ribostamycin. cf. EC 1.1.99.38, 2-deoxy-scyllo-inosamine dehydrogenase (AdoMet-dependent).
History
EC 1.1.1.329 created 2012
Pathway
ec00524  Neomycin, kanamycin and gentamicin biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K13548  2-deoxy-scyllo-inosamine dehydrogenase
Genes
SRN: A4G23_05130(neoA)
Reference
1  [PMID:16506694]
  Authors
Kudo F, Yamamoto Y, Yokoyama K, Eguchi T, Kakinuma K
  Title
Biosynthesis of 2-deoxystreptamine by three crucial enzymes in Streptomyces fradiae NBRC 12773.
  Journal
J Antibiot (Tokyo) 58:766-74 (2005)
DOI:10.1038/ja.2005.104
  Sequence
Reference
2  [PMID:19466609]
  Authors
Nepal KK, Oh TJ, Sohng JK
  Title
Heterologous production of paromamine in Streptomyces lividans TK24 using kanamycin biosynthetic genes from Streptomyces kanamyceticus ATCC12853.
  Journal
Mol Cells 27:601-8 (2009)
DOI:10.1007/s10059-009-0080-5
Other DBs
ExplorEnz - The Enzyme Database: 1.1.1.329
IUBMB Enzyme Nomenclature: 1.1.1.329
ExPASy - ENZYME nomenclature database: 1.1.1.329
BRENDA, the Enzyme Database: 1.1.1.329
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