EC 18.104.22.1689 Enzyme
D-mandelate dehydrogenase (ambiguous)
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
(R)-mandelate + NAD+ = phenylglyoxylate + NADH + H+ [RN:
The enzyme, found in bacteria and fungi, can also accept a number of substituted mandelate derivatives, such as 3-hydroxymandelate, 4-hydroxymandelate, 2-methoxymandelate, 4-hydroxy-3-methoxymandelate and 3-hydroxy-4-methoxymandelate. The enzyme has no activity with (S)-mandelate (cf. EC
, (S)-mandelate dehydrogenase) [1,2]. The enzyme transfers the pro-R-hydrogen from NADH .
EC 22.214.171.1249 created 2014
Baker, D.P. and Fewson, C.A.
Purification and characterization of D(-)-mandelate dehydrogenase from Rhodotorula graminis.
Microbiology 135:2035-2044 (1989)
Baker DP, Kleanthous C, Keen JN, Weinhold E, Fewson CA
Mechanistic and active-site studies on D(--)-mandelate dehydrogenase from Rhodotorula graminis.
Biochem J 281 ( Pt 1):211-8 (1992)
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