KEGG   ENZYME: 1.1.1.383
Entry
EC 1.1.1.383                Enzyme                                 

Name
ketol-acid reductoisomerase [NAD(P)+]
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
Sysname
(2R)-2,3-dihydroxy-3-methylbutanoate:NAD(P)+ oxidoreductase (isomerizing)
Reaction(IUBMB)
(2R)-2,3-dihydroxy-3-methylbutanoate + NAD(P)+ = (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NAD(P)H + H+ [RN:R04439 R10916]
Reaction(KEGG)
R04439 R10916
Substrate
(2R)-2,3-dihydroxy-3-methylbutanoate [CPD:C04272];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006]
Product
(2S)-2-hydroxy-2-methyl-3-oxobutanoate [CPD:C06010];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
The enzyme, characterized from the bacteria Hydrogenobaculum sp. and Syntrophomonas wolfei subsp. wolfei and from the archaea Metallosphaera sedula and Ignisphaera aggregans, can use both NADH and NADPH with similar efficiency [cf. EC 1.1.1.86, ketol-acid reductoisomerase (NADP+) and EC 1.1.1.382, ketol-acid reductoisomerase (NAD+)].
History
EC 1.1.1.383 created 2015
Reference
1  [PMID:25172159]
  Authors
Brinkmann-Chen S, Cahn JK, Arnold FH
  Title
Uncovering rare NADH-preferring ketol-acid reductoisomerases.
  Journal
Metab Eng 26:17-22 (2014)
DOI:10.1016/j.ymben.2014.08.003
  Sequence
[hya:HY04AAS1_0065] [swo:Swol_2144] [iag:Igag_1561] [mse:Msed_1927]
Other DBs
ExplorEnz - The Enzyme Database: 1.1.1.383
IUBMB Enzyme Nomenclature: 1.1.1.383
ExPASy - ENZYME nomenclature database: 1.1.1.383
BRENDA, the Enzyme Database: 1.1.1.383
CAS: 9075-02-9
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