Entry
Name
alpha-1,6-mannosyl-glycoprotein 6-beta-N-acetylglucosaminyltransferase;
MGAT5 (gene name);
N-acetylglucosaminyltransferase V;
alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase;
uridine diphosphoacetylglucosamine-alpha-mannoside beta1->6-acetylglucosaminyltransferase;
UDP-N-acetylglucosamine:alpha-mannoside-beta1,6 N-acetylglucosaminyltransferase;
alpha-1,3(6)-mannosylglycoprotein beta-1,6-N-acetylglucosaminyltransferase;
GnTV;
GlcNAc-T V;
UDP-N-acetyl-D-glucosamine:6-[2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl]-glycoprotein 6-beta-N-acetyl-D-glucosaminyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
UDP-N-acetyl-alpha-D-glucosamine:N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)-beta-D-mannosyl-glycoprotein 6-beta-N-acetyl-D-glucosaminyltransferase (configuration-inverting)
Reaction(IUBMB)
UDP-N-acetyl-alpha-D-glucosamine + beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein] = UDP + beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein] [RN:
R05991 ]
Reaction(KEGG)
Substrate
UDP-N-acetyl-alpha-D-glucosamine [CPD:
C00043 ];
beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein]
Product
UDP [CPD:
C00015 ];
beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein]
Comment
Requires Mg2+. The enzyme, found in vertebrates, participates in the processing of N-glycans in the Golgi apparatus. It catalyses the addition of N-acetylglucosamine in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides, and thus enables the synthesis of tri- and tetra-antennary complexes.
History
EC 2.4.1.155 created 1986, modified 2001, modified 2018
Pathway
Orthology
K00744 alpha-1,3(6)-mannosylglycoprotein beta-1,6-N-acetyl-glucosaminyltransferase
Genes
CCAN : 109689552 109689553
PBI : 103063095(MGAT5) 103064781
GJA : 107108668(MGAT5) 107111387
SANH : 107653587 107663499
SGH : 107586987 107593035(mgat5)
CCAR : 109047295 109083375 109087669
CAUA : 113040766 113064774 113077639
CGIB : 127942982 127987986
MASI : 127437982 127438771
TRU : 101072031(mgat5) 101074995
TFS : 130522668(mgat5) 130529365
TNG : GSTEN00006450G001 GSTEN00013473G001
NCC : 104941679(mgat5) 104962910
TBEN : 117485591 117498890(mgat5)
CGOB : 115020149(mgat5) 115026505
PGEO : 117456276(mgat5) 117466350
GACU : 117532443 117541541
EMAC : 134860808(mgat5) 134867712
ELY : 117249803(mgat5) 117251389
EFO : 125884675(mgat5) 125899720
PLEP : 121949308 121962674(mgat5)
SLUC : 116037737 116044574(mgat5)
PFLV : 114551135 114563683
GAT : 120820174(mgat5) 120833820
PPUG : 119199315(mgat5) 119228632
AFB : 129104864 129111872(mgat5)
CANA : 137783038 137794086(mgat5)
CVE : 137878957(mgat5) 137891364 137891876
MSAM : 119902006(mgat5) 119903256
LMIX : 132959306(mgat5) 132987023
ALAT : 119015474(mgat5) 119026392
ASTR : 137591307(mgat5) 137604997 137605543
CSAI : 133425058(mgat5) 133446507
XMA : 102232481(mgat5) 102236488
XCO : 114140432(mgat5) 114148135
XHE : 116715581(mgat5) 116723023
PRET : 103460330 103476254(mgat5)
PFOR : 103141415(mgat5) 103152097
PLAI : 106936559 106948945(mgat5)
PMEI : 106903323 106914659
GAF : 122827248(mgat5) 122839913
PPRL : 129374487 129379039(mgat5)
CVG : 107094135 107094137(mgat5)
CTUL : 119771852 119789711(mgat5)
NFU : 107381041 107390173(mgat5)
KMR : 108238485 108246486(mgat5)
ALIM : 106519126 106519129 106529442
NWH : 119419033 119428955(mgat5)
AOCE : 111562783 111582951
MCEP : 125000738(mgat5) 125017276
POV : 109631205 109639749(mgat5)
SSEN : 122761506 122785633
HHIP : 117754947 117775326(mgat5)
HSP : 118103351(mgat5) 118120478
SMAU : 118283105 118309346(mgat5)
LCF : 108887100 108891873(mgat5)
SDU : 111218827 111222699(mgat5)
SLAL : 111646016(mgat5) 111667400
XGL : 120798018(mgat5) 120801806
MALB : 109955689(mgat5) 109968552
BSPL : 114847546 114870384(mgat5)
SASA : 106574313 106575089
STRU : 115179283 115195944
SALP : 111953416 111971109
SFM : 108929747 108941965(mgat5)
PKI : 111849087(mgat5) 111849468
AANG : 118213644(mgat5) 118222127
AROT : 135240491(mgat5) 135250442
CCOG : 133116810(mgat5) 133123731
PSPA : 121322345 121322621
ARUT : 117407598(mgat5) 117426936
CMK : 103180379(mgat5) 103184928
CPLA : 122550236 122551703(mgat5)
HOC : 132815671 132817057(mgat5)
LERI : 129699028(mgat5) 129712590
SCLV : 120340156 120342409 120342410 120347147
CBR : CBG_12645(Cbr-gly-2)
HRO : HELRODRAFT_115589 HELRODRAFT_180890
LAK : 106160238 106160396 106171734 106172929 106177233 106181457
SMM : Smp_040510 Smp_144260
SHX : MS3_00002227(MGAT5_1)
NVE : 5509104 5517864 5517897 5517929 5521677
EPA : 110232340 110232355 110232390 110241019 110241101 110244956
ATEN : 116288093 116288103 116295791 116306769 116308527 116308528
ADF : 107327239 107337307 107345019 107345720 107345743 107347453 107353933
AMIL : 114954488 114954490 114957136 114961709
MCAZ : 138019378 138019379 138048850 138049425 138049428 138049476 138049490 138049722
MFOO : 137984258 137984861 137985291 137985533 137985538 138001903 138002032
PDAM : 113671483 113678068 113683774 113683775 113683780
SPIS : 111327556 111327561 111332554 111336901
DGT : 114517678 114520371 114522055 114527192
XEN : 124441138 124450082 124453287
HSY : 130614785 130614786 130629732 130641709
RES : 135681214 135690481 135690482
» show all
Taxonomy
Reference
Authors
Cummings RD, Trowbridge IS, Kornfeld S.
Title
A mouse lymphoma cell line resistant to the leukoagglutinating lectin from Phaseolus vulgaris is deficient in UDP-GlcNAc: alpha-D-mannoside beta 1,6 N-acetylglucosaminyltransferase.
Journal
J Biol Chem 257:13421-7 (1982)
Reference
Authors
Hindsgaul O, Tahir SH, Srivastava OP, Pierce M.
Title
The trisaccharide beta-D-GlcpNAc-(1----2)-alpha-D-Manp-(1----6)-beta-D-Manp, as its 8-methoxycarbonyloctyl glycoside, is an acceptor selective for N-acetylglucosaminyltransferase V.
Journal
Reference
Authors
Shoreibah MG, Hindsgaul O, Pierce M
Title
Purification and characterization of rat kidney UDP-N-acetylglucosamine: alpha-6-D-mannoside beta-1,6-N-acetylglucosaminyltransferase.
Journal
J Biol Chem 267:2920-7 (1992)
Reference
Authors
Gu J, Nishikawa A, Tsuruoka N, Ohno M, Yamaguchi N, Kangawa K, Taniguchi N
Title
Purification and characterization of UDP-N-acetylglucosamine: alpha-6-D-mannoside beta 1-6N-acetylglucosaminyltransferase (N-acetylglucosaminyltransferase V) from a human lung cancer cell line.
Journal
Reference
Authors
Park C, Jin UH, Lee YC, Cho TJ, Kim CH
Title
Characterization of UDP-N-acetylglucosamine:alpha-6-d-mannoside beta-1,6-N-acetylglucosaminyltransferase V from a human hepatoma cell line Hep3B.
Journal
Sequence
Reference
Authors
Saito T, Miyoshi E, Sasai K, Nakano N, Eguchi H, Honke K, Taniguchi N
Title
A secreted type of beta 1,6-N-acetylglucosaminyltransferase V (GnT-V) induces tumor angiogenesis without mediation of glycosylation: a novel function of GnT-V distinct from the original glycosyltransferase activity.
Journal
Other DBs
ExPASy - ENZYME nomenclature database: 2.4.1.155
LinkDB
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