KEGG ENZYME: 2.5.1.153

ENZYME: 2.5.1.153

Entry

EC 2.5.1.153                Enzyme

Name

adenosine tuberculosinyltransferase;
Rv3378c (locus name)

Class

Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)

Sysname

tuberculosinyl-diphosphate:adenosine tuberculosinyltransferase

Reaction(IUBMB)

tuberculosinyl diphosphate + adenosine = 1-tuberculosinyladenosine + diphosphate [RN:R12669]

Reaction(KEGG)

R12669

Substrate

tuberculosinyl diphosphate [CPD:C19836];
adenosine [CPD:C00212]

Product

1-tuberculosinyladenosine [CPD:C22319];
diphosphate [CPD:C00013]

Comment

The enzyme, characterized from the bacterial pathogen Mycobacterium tuberculosis, produces 1-tuberculosinyladenosine, an unusual terpene nucleoside that acts as a phagolysosome disruptor by neutralizing the pH, resulting in swelling of the lysosome and obliteration of its multilamellar structure.

History

EC 2.5.1.153 created 2011 as EC 3.1.7.8 and EC 3.1.7.9, transferred 2020 to EC 2.5.1.153

Orthology

K15911

adenosine tuberculosinyltransferase

Genes

ECOM:

QTN59_01130

MFB:	MFUL124B02_24610

AGE:	AA314_01782

PBRU:

LZC95_34260

MTU:

Rv3378c

MTV:	RVBD_3378c

MTC:

MT3488

MRA:

MRA_3418

MTF:	TBFG_13414

MTB:	TBMG_03429

MTK:	TBSG_03452

MTZ:	TBXG_003406

MTG:	MRGA327_20850

MTI:	MRGA423_21280

MTUR:

CFBS_3580

MTO:	MTCTRI2_3451

MTD:

UDA_3378c

MTJ:	J112_18180

MTUB:

MT7199_3426

MTUC:

J113_23650

MTUE:

J114_18115

MTX:	M943_17435

MTUH:

I917_23750

MTUL:

TBHG_03316

MTUT:

HKBT1_3567

MTUU:

HKBT2_3574

MTQ:	HKBS1_3577

MBO:	BQ2027_MB3412C

MBB:

BCG_3449c

MBT:

JTY_3449

MBM:	BCGMEX_3447c

MBK:	K60_035170

MAF:

MAF_33930

MMIC:

RN08_3726

MCE:	MCAN_34041

MCQ:	BN44_80039

MCV:	BN43_70034

MCX:	BN42_50039

MCZ:	BN45_70020

MORY:

MO_003530

MLJ:	MLAC_34860

MSHJ:

MSHI_06150

RUB:	GBA63_14800

RMAR:

GBA65_08760

» show all

Reference

1 [PMID:24516143]

Authors

Layre E, Lee HJ, Young DC, Martinot AJ, Buter J, Minnaard AJ, Annand JW, Fortune SM, Snider BB, Matsunaga I, Rubin EJ, Alber T, Moody DB.

Title

Molecular profiling of Mycobacterium tuberculosis identifies tuberculosinyl nucleoside products of the virulence-associated enzyme Rv3378c.

Journal

Proc Natl Acad Sci U S A 111:2978-83 (2014)
DOI:10.1073/pnas.1315883111

Sequence

[mtu:Rv3378c]

Reference

2 [PMID:25910243]

Authors

Young DC, Layre E, Pan SJ, Tapley A, Adamson J, Seshadri C, Wu Z, Buter J, Minnaard AJ, Coscolla M, Gagneux S, Copin R, Ernst JD, Bishai WR, Snider BB, Moody DB.

Title

In vivo biosynthesis of terpene nucleosides provides unique chemical markers of Mycobacterium tuberculosis infection.

Journal

Chem Biol 22:516-526 (2015)
DOI:10.1016/j.chembiol.2015.03.015

Sequence

[mtu:Rv3378c]

Reference

3 [PMID:31427817]

Authors

Buter J, Cheng TY, Ghanem M, Grootemaat AE, Raman S, Feng X, Plantijn AR, Ennis T, Wang J, Cotton RN, Layre E, Ramnarine AK, Mayfield JA, Young DC, Jezek Martinot A, Siddiqi N, Wakabayashi S, Botella H, Calderon R, Murray M, Ehrt S, Snider BB, Reed MB, Oldfield E, Tan S, Rubin EJ, Behr MA, van der Wel NN, Minnaard AJ, Moody DB.

Title

Mycobacterium tuberculosis releases an antacid that remodels phagosomes.

Journal

Nat Chem Biol 15:889-899 (2019)
DOI:10.1038/s41589-019-0336-0

Sequence

[mtu:Rv3378c]

Other DBs

ExplorEnz - The Enzyme Database:

2.5.1.153

IUBMB Enzyme Nomenclature:

2.5.1.153

ExPASy - ENZYME nomenclature database:

2.5.1.153

BRENDA, the Enzyme Database:

2.5.1.153

LinkDB

DBGET integrated database retrieval system