KEGG   ENZYME: 2.5.1.153
Entry
EC 2.5.1.153                Enzyme                                 

Name
adenosine tuberculosinyltransferase;
Rv3378c (locus name)
Class
Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
Sysname
tuberculosinyl diphosphate:adenosine tuberculosinyltransferase
Reaction(IUBMB)
tuberculosinyl diphosphate + adenosine = 1-tuberculosinyladenosine + diphosphate [RN:R12669]
Reaction(KEGG)
R12669
Substrate
tuberculosinyl diphosphate [CPD:C19836];
adenosine [CPD:C00212]
Product
1-tuberculosinyladenosine [CPD:C22319];
diphosphate [CPD:C00013]
Comment
The enzyme, characterized from the bacterial pathogen Mycobacterium tuberculosis, produces 1-tuberculosinyladenosine, an unusual terpene nucleoside that acts as a phagolysosome disruptor by neutralizing the pH, resulting in swelling of the lysosome and obliteration of its multilamellar structure.
History
EC 2.5.1.153 created 2011 as EC 3.1.7.8 and EC 3.1.7.9, transferred 2020 to EC 2.5.1.153
Orthology
K15911  adenosine tuberculosinyltransferase
Genes
MFB: MFUL124B02_24610
AGE: AA314_01782
MTU: Rv3378c
MTV: RVBD_3378c
MTC: MT3488
MRA: MRA_3418
MTF: TBFG_13414
MTB: TBMG_03429
MTK: TBSG_03452
MTZ: TBXG_003406
MTUR: CFBS_3580
MTD: UDA_3378c
MTUC: J113_23650
MTUE: J114_18115
MTUH: I917_23750
MTUL: TBHG_03316
MTUT: HKBT1_3567
MTUU: HKBT2_3574
MBB: BCG_3449c
MBT: JTY_3449
MAF: MAF_33930
MMIC: RN08_3726
 » show all
Reference
1  [PMID:24516143]
  Authors
Layre E, Lee HJ, Young DC, Martinot AJ, Buter J, Minnaard AJ, Annand JW, Fortune SM, Snider BB, Matsunaga I, Rubin EJ, Alber T, Moody DB.
  Title
Molecular profiling of Mycobacterium tuberculosis identifies tuberculosinyl nucleoside products of the virulence-associated enzyme Rv3378c.
  Journal
Proc Natl Acad Sci U S A 111:2978-83 (2014)
DOI:10.1073/pnas.1315883111
  Sequence
[mtu:Rv3378c]
Reference
2  [PMID:25910243]
  Authors
Young DC, Layre E, Pan SJ, Tapley A, Adamson J, Seshadri C, Wu Z, Buter J, Minnaard AJ, Coscolla M, Gagneux S, Copin R, Ernst JD, Bishai WR, Snider BB, Moody DB.
  Title
In vivo biosynthesis of terpene nucleosides provides unique chemical markers of Mycobacterium tuberculosis infection.
  Journal
Chem Biol 22:516-526 (2015)
DOI:10.1016/j.chembiol.2015.03.015
  Sequence
[mtu:Rv3378c]
Reference
3  [PMID:31427817]
  Authors
Buter J, Cheng TY, Ghanem M, Grootemaat AE, Raman S, Feng X, Plantijn AR, Ennis T, Wang J, Cotton RN, Layre E, Ramnarine AK, Mayfield JA, Young DC, Jezek Martinot A, Siddiqi N, Wakabayashi S, Botella H, Calderon R, Murray M, Ehrt S, Snider BB, Reed MB, Oldfield E, Tan S, Rubin EJ, Behr MA, van der Wel NN, Minnaard AJ, Moody DB.
  Title
Mycobacterium tuberculosis releases an antacid that remodels phagosomes.
  Journal
Nat Chem Biol 15:889-899 (2019)
DOI:10.1038/s41589-019-0336-0
  Sequence
[mtu:Rv3378c]
Other DBs
ExplorEnz - The Enzyme Database: 2.5.1.153
IUBMB Enzyme Nomenclature: 2.5.1.153
ExPASy - ENZYME nomenclature database: 2.5.1.153
BRENDA, the Enzyme Database: 2.5.1.153
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