| Entry |
|
|---|
| Name |
|
|---|
| Class |
Hydrolases;
Acting on ester bonds;
Exoribonucleases producing 5'-phosphomonoesters
 |
|---|
| Reaction(IUBMB) |
Exonucleolytic cleavage that removes extra residues from the 3'-terminus of tRNA to produce 5'-mononucleotides
|
|---|
| Comment |
Requires divalent cations for activity (Mg2+, Mn2+ or Co2+). Alteration of the 3'-terminal base has no effect on the rate of hydrolysis whereas modification of the 3'-terminal sugar has a major effect. tRNA terminating with a 3'-phosphate is completely inactive [3]. This enzyme can convert a tRNA precursor into a mature tRNA [2].
|
|---|
| History |
EC 3.1.13.5 created 2006
|
|---|
| Orthology |
|
|---|
| Genes |
» show all
 |
|---|
| Reference |
|
|---|
| Authors |
Ghosh RK, Deutscher MP. |
|---|
| Title |
Identification of an Escherichia coli nuclease acting on structurally altered transfer RNA molecules. |
|---|
| Journal |
J Biol Chem 253:997-1000 (1978) |
|---|
| Reference |
|
|---|
| Authors |
Cudny H, Zaniewski R, Deutscher MP. |
|---|
| Title |
Escherichia coli RNase D. Purification and structural characterization of a putative processing nuclease. |
|---|
| Journal |
J Biol Chem 256:5627-32 (1981) |
|---|
| Reference |
|
|---|
| Authors |
Cudny H, Zaniewski R, Deutscher MP. |
|---|
| Title |
Escherichia coli RNase D. Catalytic properties and substrate specificity. |
|---|
| Journal |
J Biol Chem 256:5633-7 (1981) |
|---|
| Sequence |
|
|---|
| Reference |
|
|---|
| Authors |
Zhang JR, Deutscher MP. |
|---|
| Title |
Cloning, characterization, and effects of overexpression of the Escherichia coli rnd gene encoding RNase D. |
|---|
| Journal |
|
|---|
| Other DBs |
| ExplorEnz - The Enzyme Database: | 3.1.13.5 |
| ExPASy - ENZYME nomenclature database: | 3.1.13.5 |
|
|---|
| LinkDB |
 |
|---|
