KEGG   ENZYME: 3.2.1.196
Entry
EC 3.2.1.196                Enzyme                                 

Name
limit dextrin alpha-1,6-maltotetraose-hydrolase;
glgX (gene name);
glycogen debranching enzyme (ambiguous)
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
Sysname
glycogen phosphorylase-limit dextrin maltotetraose-hydrolase
Reaction(IUBMB)
Hydrolysis of (1->6)-alpha-D-glucosidic linkages to branches with degrees of polymerization of three or four glucose residues in limit dextrin.
Comment
This bacterial enzyme catalyses a reaction similar to EC 3.2.1.33, amylo-alpha-1,6-glucosidase (one of the activities of the eukaryotic glycogen debranching enzyme). However, while EC 3.2.1.33 removes single glucose residues linked by 1,6-alpha-linkage, and thus requires the additional activity of 4-alpha-glucanotransferase (EC 2.4.1.25) to act on limit dextrins formed by glycogen phosphorylase (EC 2.4.1.1), this enzyme removes maltotriose and maltotetraose chains that are attached by 1,6-alpha-linkage to the limit dextrin main chain, generating a debranched limit dextrin without a need for another enzyme.
History
EC 3.2.1.196 created 2016
Orthology
K02438  glycogen debranching enzyme
Genes
ECO: b3431(glgX)
ECJ: JW3394(glgX)
ECD: ECDH10B_3605(glgX)
EBW: BWG_3123(glgX)
ECOK: ECMDS42_2876(glgX)
ECE: Z4794(glgX)
ECS: ECs4276
ECF: ECH74115_4743(glgX)
ETW: ECSP_4385(glgX)
ELX: CDCO157_4017
EOI: ECO111_4242(glgX)
EOJ: ECO26_4521(glgX)
EOH: ECO103_4152(glgX)
ECOO: ECRM13514_4383(glgX)
ECOH: ECRM13516_4180(glgX)
ESL: O3K_01895
ESO: O3O_23755
ESM: O3M_01940
ECK: EC55989_3841(glgX)
ECG: E2348C_3677(glgX)
EOK: G2583_4132(glgX)
ELH: ETEC_3680
ECW: EcE24377A_3910(glgX)
EUN: UMNK88_4201(glgX)
ECP: ECP_3525
ENA: ECNA114_3541(glgX)
ECOS: EC958_3825(glgX)
ECV: APECO1_3026(glgX)
ECX: EcHS_A3631(glgX)
ECM: EcSMS35_3713(glgX)
ECY: ECSE_3700
ECR: ECIAI1_3577(glgX)
ECQ: ECED1_4106(glgX)
EUM: ECUMN_3895(glgX)
ECT: ECIAI39_3912(glgX)
EOC: CE10_3953(glgX)
EBR: ECB_03283(glgX)
EBL: ECD_03283(glgX)
EBE: B21_03236(glgX)
EBD: ECBD_0311
ECI: UTI89_C3940(glgX)
EIH: ECOK1_3856(glgX)
ECZ: ECS88_3829(glgX)
ECC: c4218(glgX)
ELO: EC042_3698(glgX)
ESE: ECSF_3252
EKF: KO11_05610(glgX)
EAB: ECABU_c38600(glgX)
EDJ: ECDH1ME8569_3309(glgX)
ELW: ECW_m3691(glgX)
ELL: WFL_18035(glgX)
ELC: i14_3889(glgX)
ELD: i02_3889(glgX)
ELP: P12B_c3533(glgX)
ELF: LF82_0841(glgX)
ECOI: ECOPMV1_03747(glgX)
ECOJ: P423_19115
EFE: EFER_3408(glgX)
EAL: EAKF1_ch2528(glgX)
ESZ: FEM44_08125(glgX)
STY: STY4273(glgX)
STT: t3983(glgX)
STM: STM3537(glgX)
SEO: STM14_4257(glgX)
SEY: SL1344_3504(glgX)
SEJ: STMUK_3522(glgX)
SEB: STM474_3704(glgX)
SEF: UMN798_3840(glgX)
SENR: STMDT2_34241(glgX)
SEND: DT104_35201(glgX)
SENI: CY43_18375
SPT: SPA3388(glgX)
SEK: SSPA3163
SEI: SPC_3606(glgX)
SEC: SCH_3467(glgX)
SEH: SeHA_C3846(glgX)
SHB: SU5_04012
SEE: SNSL254_A3804(glgX)
SEW: SeSA_A3727(glgX)
SEA: SeAg_B3738(glgX)
SENS: Q786_17260
SED: SeD_A3907(glgX)
SEG: SG3900(glgX)
SEL: SPUL_4043(glgX)
SEGA: SPUCDC_4029(glgX)
SET: SEN3361(glgX)
SENA: AU38_17150
SENO: AU37_17340
SENV: AU39_17345
SENQ: AU40_19315
SENL: IY59_17775
SENB: BN855_36100(glgX)
SENE: IA1_17150
SBG: SBG_3131
SBZ: A464_3605
SALZ: EOS98_01625(glgX)
SFL: SF3454(glgX)
SFX: S4309(glgX)
SFV: SFV_3440(glgX)
SFE: SFxv_3771(glgX)
SFN: SFy_4914
SFS: SFyv_4986
SFT: NCTC1_03735(glgX)
SSN: SSON_3671(glgX)
SBO: SBO_3429(glgX)
SBC: SbBS512_E3893(glgX)
SDY: SDY_3577(glgX)
ENC: ECL_04793
ECLX: LI66_21100
ECLY: LI62_23085
ECLZ: LI64_20185
ECLO: ENC_26250
EEC: EcWSU1_04213(glgX)
ECHG: FY206_22910(glgX)
EBG: FAI37_08290(glgX)
ESA: ESA_04310
CSK: ES15_0241(glgX)
CTU: CTU_39400(glgX)
KPN: KPN_03797(glgX)
KPU: KP1_5130(glgX)
KPP: A79E_0317
KPT: VK055_3674(glgX)
KPR: KPR_5120(glgX)
KPJ: N559_0357
KPX: PMK1_01300(glgX)
KPNU: LI86_01630
KPNK: BN49_0319(glgX)
KVA: Kvar_0303
KPE: KPK_0317(glgX)
KOX: KOX_04730
KOE: A225_5409
EAE: EAE_05390
EAR: CCG32920
KQV: B8P98_01645(glgX)
KLW: DA718_02080(glgX)
CRO: ROD_43941(glgX)
CKO: CKO_04850
CYO: CD187_01445(glgX)
CPOT: FOB25_12895(glgX)
CAMA: F384_18650
CFAR: CI104_01550(glgX)
EBT: EBL_c02040(glgX)
RTG: NCTC13098_00403(glgX_1)
REE: electrica_00302(glgX)
CLAP: NCTC11466_00138(glgX)
KOT: EH164_20535(glgX)
KIE: NCTC12125_04220(glgX)
LER: GNG29_21360(glgX)
LEA: GNG26_20565(glgX)
LNI: CWR52_14050(glgX)
BUF: D8682_16915(glgX)
BAGE: BADSM9389_02700(glgX)
METY: MRY16398_03690(glgX)
AHN: NCTC12129_00351(glgX_1)
YRE: HEC60_16535(glgX)
SGOE: A8O29_002070(glgX)
PDZ: HHA33_04050(glgX)
EBF: D782_0286
PSTS: E05_21500
YPE: YPO3941(glgX)
YPK: y3887(glgX)
YPH: YPC_4440(glgX)
YPA: YPA_3770
YPN: YPN_3589
YPM: YP_3303(glgX)
YPG: YpAngola_A4119(glgX)
YPZ: YPZ3_2217(glgX)
YPD: YPD4_3469(glgX)
YPX: YPD8_3471(glgX)
YPW: CH59_1888(glgX)
YPJ: CH55_2620(glgX)
YPV: BZ15_3764(glgX)
YPL: CH46_1117(glgX)
YPS: YPTB3786(glgX)
YPO: BZ17_2799(glgX)
YPI: YpsIP31758_4005(glgX)
YPY: YPK_0148
YPB: YPTS_3983
YPQ: DJ40_2609(glgX)
YPU: BZ21_3120(glgX)
YPR: BZ20_2308(glgX)
YPC: BZ23_3411(glgX)
YPF: BZ19_3270(glgX)
YEN: YE4012(glgX)
YEY: Y11_31851
YEW: CH47_3720(glgX)
YET: CH48_1463(glgX)
YEE: YE5303_38431(glgX)
YAL: AT01_2662(glgX)
YFR: AW19_3444(glgX)
YIN: CH53_1579(glgX)
YKR: CH54_2346(glgX)
YRO: CH64_2426(glgX)
YRU: BD65_2118(glgX)
YHI: D5F51_21215(glgX)
YCA: F0T03_20870(glgX)
YMO: HRD69_06410(glgX)
SMAR: SM39_4103(glgX)
SMAC: SMDB11_3890(glgX)
SMW: SMWW4_v1c45970(glgX)
SPE: Spro_4646
SRL: SOD_c44440(glgX)
SPLY: Q5A_024185(glgX)
SERF: L085_03250
SQU: E4343_17525(glgX)
SFJ: SAMEA4384070_4603(glgX)
SOF: NCTC11214_01407(glgX)
RAA: Q7S_01020
EAME: GXP68_00855(glgX)
ECA: ECA4150(glgX)
PATR: EV46_20665
PATO: GZ59_42060(glgX)
PCT: PC1_3936
PEC: W5S_4293
PPUJ: E2566_20035(glgX)
SOD: Sant_0351(glgX1)
DDD: Dda3937_00331(glgX)
DDQ: DDI_3942
DAQ: DAQ1742_04160(glgX)
DIC: Dpoa569_000316(glgX)
BNG: EH206_01685(glgX)
EAM: EAMY_3468(glgX)
EAY: EAM_3271(glgX)
ETA: ETA_32480(glgX)
EPY: EpC_34590(glgX)
EPR: EPYR_03717(glgX)
EBI: EbC_42220(glgX)
ERJ: EJP617_09450(glgX)
EGE: EM595_0294(glgX1)
EPE: CI789_20085(glgX)
ERWI: GN242_01390(glgX)
PAM: PANA_3700(glgX)
PLF: PANA5342_0345(glgX1)
PAJ: PAJ_2924(glgX)
PVA: Pvag_2980(glgx3)
PSTW: DSJ_01110
PANS: FCN45_01320(glgX)
PEY: EE896_01690(glgX)
PDIS: D8B20_01185(glgX)
MINT: C7M51_03800(glgX_2)
MTHI: C7M52_03226(glgX_2)
PVG: CRN77_12775(glgX)
PHAU: PH4a_15470(glgX)
PCIB: F9282_09505(glgX)
PCOL: F1325_09510(glgX)
PALA: CO695_06685(glgX)
PHEI: NCTC12003_03508(glgX)
PRJ: NCTC6933_03516(glgX)
ETR: ETAE_3308(glgX)
ETD: ETAF_2996
ETE: ETEE_1536(treX)
PRAG: EKN56_10065(glgX)
LRI: NCTC12151_02115(glgX)
HIN: HI1358(glgX)
HIT: NTHI1808(glgX)
HIU: HIB_15270
HIE: R2846_0837(glgX)
HIZ: R2866_1044(glgX)
HIK: HifGL_001152(glgX)
HIA: H733_1097(glgX)
HIW: NTHI477_00304(glgX)
HIX: NTHI723_00222(glgX)
HAY: C3V42_08275(glgX)
HPIT: NCTC13334_01553(glgX)
HHZ: NCTC10839_01540(glgX)
HAP: HAPS_0291(glgX)
HPAZ: K756_05105
HPAS: JL26_04595
HPAK: JT17_02150
GLE: CJD39_03225(glgX)
HSO: HS_0888(glgX)
HSM: HSM_1367
PMU: PM0542(glgx)
PMV: PMCN06_0572(pulA)
PMP: Pmu_06080(glgX)
PMUL: DR93_1319(glgX)
PDAG: 4362423_00820(glgX)
MSU: MS1122(glgX)
MHT: D648_10260(glgX)
MHQ: D650_17310(glgX)
MHAT: B824_10690(glgX)
MHX: MHH_c16280(glgX)
MHAE: F382_13165
MHAM: J450_11775
MHAO: J451_13400
MHAL: N220_05355
MHAQ: WC39_08605
MHAY: VK67_08605
MVR: X781_8940
MVI: X808_7690
MVG: X874_7800
APL: APL_0347(glgX)
APJ: APJL_0363(glgX)
APA: APP7_0352(glgX)
ASU: Asuc_1353
APOR: DDU33_08320(glgX)
AIO: EXH44_10745(glgX)
ALIG: NCTC10568_01176(glgX)
AAT: D11S_1310
AAN: D7S_00080
AACN: AANUM_1765
ASEG: NCTC10977_01407(glgX)
BTO: WQG_12300
BTRE: F542_9740
BTRH: F543_11100
BTRA: F544_12680
AVT: NCTC3438_01467(glgX)
RPNE: NCTC8284_03374(glgX_2)
RHEY: FEE42_05470(glgX)
PAET: NCTC13378_00665(glgX)
CHJ: NCTC10426_00807(glgX)
NSI: A6J88_10510(glgX)
NMJ: NM96_04840(glgX)
NEI: BG910_02400(glgX)
NFV: FAH67_02135(glgX)
NAQ: D0T90_10075(glgX)
NBL: GJV52_09850(glgX)
IOD: EJO50_01485(glgX)
DEE: HQN60_01900(glgX)
CHIZ: HQ393_08605(glgX)
CFON: HZU75_12860(glgX)
BTQ: BTQ_4226(glgX)
BTJ: BTJ_5264(glgX)
BTZ: BTL_3725(glgX)
BTV: BTHA_4158(glgX)
BTHE: BTN_3934(glgX)
BTHM: BTRA_3722(glgX)
BTHA: DR62_3976
BTHL: BG87_3682(glgX)
HYF: DTO96_100655(glgX)
RFR: Rfer_0515
PNA: Pnap_1103
VPD: VAPA_1c04940(glgX1)
VBO: CKY39_02385(glgX)
VAM: C4F17_16585(glgX)
CBX: Cenrod_1454(glgX)
HYR: BSY239_3560(glgX)
LCH: Lcho_1887
TIN: Tint_1096
THI: THI_1388(glgX)
RGE: RGE_29100(glgX)
SNN: EWH46_08585(glgX)
THK: CCZ27_07370(glgX)
MLO: mlr7591
MESM: EJ066_16930(glgX)
MESP: C1M53_01050(glgX)
MHUA: MCHK_1055(glgX)
MJR: EB229_28330(glgX)
MERD: EB233_24670(glgX)
AMIH: CO731_04473(glgX)
SME: SMc03926(glgX1)
SMX: SM11_chr3015(glgX1)
SMI: BN406_02704(glgX)
SMEL: SM2011_c03926(glgX1)
SMER: DU99_15725
SMD: Smed_2744
SFH: SFHH103_02906(glgX1)
SFD: USDA257_c53160(glgX2)
SIX: BSY16_3830(glgX)
SAME: SAMCFNEI73_Ch3273(glgX)
EAD: OV14_0352
EMX: FKV68_18015(glgX)
ATU: Atu4073(glgX)
ARA: Arad_3874(glgX)
ATF: Ach5_38530(glgX)
AVI: Avi_3764(glgX)
AGC: BSY240_3938(glgX)
ARO: B0909_17395(glgX)
AGT: EYD00_19660(glgX)
ALF: CFBP5473_03010(glgX)
RET: RHE_CH03598(glgXch)
REC: RHECIAT_CH0003866(glgXch)
REL: REMIM1_CH03673(glgX-1)
REP: IE4803_CH03977(glgX-1)
REI: IE4771_CH03915(glgX-1)
RLE: RL4119(glgX)
RLG: Rleg_3654
RTR: RTCIAT899_CH15315(glgX)
RIR: BN877_II1203(glgX)
RPUS: CFBP5875_19870(glgX)
RHL: LPU83_3596(glgX)
RGA: RGR602_CH03501(glgX-1)
RHN: AMJ98_CH03824(glgX-1)
RPHA: AMC79_CH03779(glgX-1)
RHT: NT26_2756(glgX)
RHX: AMK02_CH03713(glgX-1)
RHK: Kim5_CH03953(glgX-1)
REZ: AMJ99_CH03877(glgX-1)
RJG: CCGE525_17795(glgX)
RHR: CKA34_18145(glgX)
RGR: FZ934_13690(glgX)
RAD: CO657_17245(glgX)
ROY: G3A56_22555(glgX)
NGL: RG1141_CH30490(glgX)
NGG: RG540_CH31240(glgX)
NEO: CYG48_11150(glgX)
NEN: NCHU2750_28720(glgX)
SHZ: shn_15530
BOS: BSY19_3444(glgX)
SNO: Snov_2556
STAR: G3545_27555(glgX)
LNE: FZC33_29115(glgX)
ANC: GBB76_07500(glgX)
APRA: G3A50_04270(glgX)
MSL: Msil_0211
RHJ: HZY79_09585(malQ)
PHL: KKY_2043
DEI: C4375_14375(glgX)
DEA: FPZ08_09660(glgX)
BVR: BVIR_108
BLAG: BLTE_33320
YTI: FNA67_15195(glgX)
MSC: BN69_1966(glgX)
MROS: EHO51_15400(glgX)
MHEY: H2LOC_015590(glgX)
MPAR: F7D14_06690(glgX)
MMED: Mame_03846(glgX)
NOH: G5V57_32790(glgX)
MON: G8E03_06440(glgX)
HBA: Hbal_1874
NAR: Saro_1654
NOV: TQ38_013060(glgX)
NOT: C7W88_10995(glgX)
NOR: FA702_13630(glgX)
STAX: MC45_03465
SPAU: DRN02_009750(glgX)
SJP: SJA_C1-21930(glgX)
SSY: SLG_08630(glgX)
SPMI: K663_07105
SPHB: EP837_00339(treX)
SPHR: BSY17_2617(glgX)
SINB: SIDU_04395
SPHT: K426_12140
SHYD: CJD35_14630(glgX)
SYA: A6768_04170(glgX)
SUFL: FIL70_05345(glgX)
RDI: CMV14_12310(glgX)
ADO: A6F68_02508(glgX)
ALH: G6N82_02510(glgX)
PHZ: CHX26_06410(glgX)
POT: E2E27_04440(glgX)
RCE: RC1_2119(treZ)
AZL: AZL_a05620(malQ)
AOZ: HUE56_24320(treZ)
MGM: Mmc1_1514
LEIF: HF024_08665(glgX)
LSE: F1C12_04500(glgX)
CMI: CMM_1401(glgX)
CMS: CMS1046
CMC: CMN_01373(glgX)
MTS: MTES_3288
MHOS: CXR34_09650(glgX)
MFOL: DXT68_07735(glgX)
MOO: BWL13_01505(glgX_2)
MLV: CVS47_01621(glgX_2)
MWA: E4K62_10115(glgX)
MOY: CVS54_01987(glgX_1)
AQG: HRU87_02535(glgX)
RRY: C1O28_06640(glgX)
RIA: C7V51_05085(glgX)
RFS: C1I64_05085(glgX)
RTE: GSU10_08160(glgX)
AGM: DCE93_08755(glgX)
AGF: ET445_11150(glgX)
CRY: B7495_10060(glgX)
AMIN: AUMI_17870
AUW: AURUGA1_00585(glgX)
MYL: C3E77_08820(glgX)
SALC: C2138_08540(glgX)
SALD: FVA74_08250(glgX)
HUM: DVJ78_14650(glgX)
HUW: FPZ11_04105(glgX)
GRY: D7I44_00245(glgX)
LYD: D7I47_10450(glgX)
LYK: FLP23_11250(glgX)
PLAP: EAO79_12220(glgX)
HEA: HL652_04050(glgX)
FRN: F1C15_14830(glgX)
GLN: F1C58_10860(glgX)
CHRE: IE160_09115(glgX)
ART: Arth_0733
ARR: ARUE_c08570(glgX1)
ARM: ART_3324
ARN: CGK93_04590(glgX)
ARX: ARZXY2_3125(treX)
ARTH: C3B78_03465(glgX)
ARTP: E5206_19075(glgX)
AAU: AAur_0904(glgX)
PUE: FV140_04610(glgX)
ACH: Achl_0857
PSNI: NIBR502771_01105(glgX)
PSEY: GU243_20100(glgX)
KRH: KRH_18670
KII: KocCE7_10020(glgX)
KRS: EQG70_13950(glgX)
KOD: HBK84_09960(glgX)
AUL: DCC27_007250(glgX)
CIG: E7741_12180(glgX)
PAC: PPA1115
PAK: HMPREF0675_4177(glgX)
PAW: PAZ_c11640(glgX)
PACC: PAC1_05840
PACH: PAGK_1038
CACN: RN83_05945
CGRN: 4412665_01374(glgX)
PFR: PFREUD_10700(glgX_treX)
PFRE: RM25_1105
PACD: EGX94_00125(glgX)
PAUS: NCTC13651_01211(glgX)
PPC: HMPREF9154_1704(glgX)
ACIJ: JS278_01566(glgX)
AJI: C0Z10_06910(glgX)
MPH: MLP_29690(glgX)
MIK: FOE78_14710(glgX)
MICG: GJV80_05615(glgX)
PRV: G7070_16865(glgX)
NCA: Noca_1812
MGG: MPLG2_1116(glgX)
FAL: FRAAL5883(glgX)
 » show all
Reference
1  [PMID:779849]
  Authors
Jeanningros R, Creuzet-Sigal N, Frixon C, Cattaneo J
  Title
Purification and properties of a debranching enzyme from Escherichia coli.
  Journal
Biochim Biophys Acta 438:186-99 (1976)
DOI:10.1016/0005-2744(76)90235-7
Reference
2  [PMID:15687211]
  Authors
Dauvillee D, Kinderf IS, Li Z, Kosar-Hashemi B, Samuel MS, Rampling L, Ball S, Morell MK
  Title
Role of the Escherichia coli glgX gene in glycogen metabolism.
  Journal
J Bacteriol 187:1465-73 (2005)
DOI:10.1128/JB.187.4.1465-1473.2005
Reference
3  [PMID:20187119]
  Authors
Song HN, Jung TY, Park JT, Park BC, Myung PK, Boos W, Woo EJ, Park KH
  Title
Structural rationale for the short branched substrate specificity of the glycogen debranching enzyme GlgX.
  Journal
Proteins 78:1847-55 (2010)
DOI:10.1002/prot.22697
  Sequence
[eco:b3431]
Other DBs
ExplorEnz - The Enzyme Database: 3.2.1.196
IUBMB Enzyme Nomenclature: 3.2.1.196
ExPASy - ENZYME nomenclature database: 3.2.1.196
BRENDA, the Enzyme Database: 3.2.1.196
LinkDB

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