KEGG   ENZYME: 3.2.1.209
Entry
EC 3.2.1.209                Enzyme                                 

Name
endoplasmic reticulum Man9GlcNAc2 1,2-alpha-mannosidase;
MAN1B1 (gene name);
MNS1 (gene name);
MNS3 (gene name)
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
Sysname
Man9GlcNAc2-[protein]2-alpha-mannohydrolase (configuration-inverting)
Reaction(IUBMB)
Man9GlcNAc2-[protein] + H2O = Man8GlcNAc2-[protein] (isomer 8A1,2,3B1,3) + D-mannopyranose [RN:R12477]
Reaction(KEGG)
R12477(G);
(other) R06722(G)
Substrate
Man9GlcNAc2-[protein];
H2O [CPD:C00001]
Product
Man8GlcNAc2-[protein] (isomer 8A1,2,3B1,3);
D-mannopyranose [CPD:C00159]
Comment
The enzyme, located in the endoplasmic reticulum, primarily trims a single alpha-1,2-linked mannose residue from Man9GlcNAc2 to produce Man8GlcNAc2 isomer 8A1,2,3B1,3 (the names of the isomers listed here are based on a nomenclature system proposed by Prien et al [7]). The removal of the single mannosyl residue occurs in all eukaryotes as part of the processing of N-glycosylated proteins, and is absolutely essential for further elongation of the outer chain of properly-folded N-glycosylated proteins in yeast. In addition, the enzyme is involved in glycoprotein quality control at the ER quality control compartment (ERQC), helping to target misfolded glycoproteins for degradation. When present at very high concentrations in the ERQC, the enzyme can trim the carbohydrate chain further to Man(5-6)GlcNAc2.
History
EC 3.2.1.209 created 2019
Pathway
ec00510  N-Glycan biosynthesis
ec00513  Various types of N-glycan biosynthesis
ec01100  Metabolic pathways
Orthology
K23741  endoplasmic reticulum Man9GlcNAc2 1,2-alpha-mannosidase
Genes
HSA: 11253(MAN1B1)
PTR: 473099(MAN1B1)
PPS: 100987343(MAN1B1)
GGO: 101134859(MAN1B1)
PON: 100432279(MAN1B1)
NLE: 100594686(MAN1B1)
MCC: 706918(MAN1B1)
MCF: 101867098(MAN1B1)
CSAB: 103239582(MAN1B1)
RRO: 104669636(MAN1B1)
RBB: 108542887(MAN1B1)
CJC: 100392624(MAN1B1)
SBQ: 101032054(MAN1B1)
MMU: 227619(Man1b1)
MCAL: 110289782(Man1b1)
MPAH: 110317719(Man1b1)
RNO: 499751(Man1b1)
MUN: 110556996(Man1b1)
CGE: 100756512
NGI: 103732606 103741172(Man1b1)
HGL: 101724891(Man1b1)
CCAN: 109697987(Man1b1)
TUP: 102470543(MAN1B1)
VVP: 112911405(MAN1B1)
AML: 100478118
UMR: 103675308(MAN1B1)
PTG: 102948947(MAN1B1)
PPAD: 109260237(MAN1B1)
AJU: 106989099(MAN1B1) 113597246
BTA: 100336900(MAN1B1) 107131137(MAN1B1)
CHX: 102182048(MAN1B1) 106501835
OAS: 105605821 105605822(MAN1B1)
SSC: 100627465(MAN1B1)
CFR: 102513732(MAN1B1)
CDK: 105106772(MAN1B1) 105106783
BACU: 103003282 103008754(MAN1B1)
LVE: 103086557(MAN1B1)
OOR: 101275150(MAN1B1)
DLE: 111176994(MAN1B1)
ECB: 100147103(MAN1B1) 100147680
EPZ: 103568009(MAN1B1)
EAI: 106845522(MAN1B1)
MYB: 102240083(MAN1B1)
MYD: 102775495(MAN1B1)
MNA: 107536151(MAN1B1)
HAI: 109381800(MAN1B1)
DRO: 112306395(MAN1B1)
PALE: 102890593(MAN1B1)
RAY: 107503110(MAN1B1)
MJV: 108406826(MAN1B1)
LAV: 100664627(MAN1B1) 100670902
TMU: 101354994
MDO: 100022731(MAN1B1)
SHR: 100918683(MAN1B1)
PCW: 110205295(MAN1B1)
OAA: 100080650(MAN1B1)
GGA: 417296(MAN1B1)
MGP: 100543997(MAN1B1)
CJO: 107321628(MAN1B1)
NMEL: 110407012(MAN1B1)
APLA: 101803362(MAN1B1)
ACYG: 106037118(MAN1B1)
TGU: 100223461(MAN1B1)
LSR: 110472180(MAN1B1)
SCAN: 103819092(MAN1B1)
GFR: 102031555(MAN1B1)
FAB: 101816081(MAN1B1)
PHI: 102103624(MAN1B1)
PMAJ: 107212037(MAN1B1)
CCAE: 111936881(MAN1B1)
CCW: 104688195(MAN1B1)
ETL: 114064126(MAN1B1)
FPG: 101914433(MAN1B1)
FCH: 102045806(MAN1B1)
CLV: 102083787(MAN1B1)
EGZ: 104124017(MAN1B1)
NNI: 104018375(MAN1B1)
ACUN: 113486627(MAN1B1)
PADL: 103915501(MAN1B1)
AAM: 106494264(MAN1B1)
ASN: 102387590(MAN1B1)
AMJ: 102567138(MAN1B1)
PSS: 102455673(MAN1B1)
CMY: 102942408(MAN1B1)
CPIC: 101947106(MAN1B1)
ACS: 100562395(man1b1)
PVT: 110078803(MAN1B1)
PBI: 103052908(MAN1B1)
PMUR: 107295058(MAN1B1)
TSR: 106542597(MAN1B1)
PMUA: 114589499(MAN1B1)
GJA: 107123282(MAN1B1)
XLA: 447291(man1b1.L)
XTR: 100145194(man1b1)
NPR: 108797130(MAN1B1)
DRE: 556237(man1b1b) 562592(man1b1a)
IPU: 108255760(man1b1) 108279088
AMEX: 103022471(man1b1) 103024141
EEE: 113573453 113577930(man1b1)
LCO: 104923806 104930877(man1b1)
NCC: 104944586(man1b1)
MZE: 101475381(man1b1)
OLA: 101156826(man1b1) 101168136
XMA: 102232900(man1b1) 102236792
NFU: 107375119
KMR: 108241416 108245625(man1b1)
POV: 109639093(man1b1) 109641232
LCF: 108878392(man1b1) 108889011
SDU: 111221245 111226918(man1b1)
SLAL: 111647914(man1b1) 111650117
HCQ: 109527092(man1b1)
BPEC: 110154656(man1b1) 110164288
SFM: 108922690 108938629(man1b1)
PKI: 111854001 111857531(man1b1)
LCM: 102347754(MAN1B1)
CMK: 103180403(man1b1)
RTP: 109927301(man1b1)
BFO: 118420800
CIN: 100180827
APLC: 110984825
SKO: 100378677
DME: Dmel_CG11874(alpha-Man-Ib)
DER: 6555014
DSE: 6612651
DSI: Dsimw501_GD17996(Dsim_GD17996)
DAN: 6501495
DSR: 110177400
DPE: 6587772
DMN: 108155025
DAZ: 108609054
DNV: 108656217
DHE: 111599442
DVI: 6632758
MDE: 101889535
LCQ: 111690671
AALB: 109415549
AME: 413579
BIM: 100746318
BTER: 100651817
CCAL: 108622226
OBB: 114874226
SOC: 105204046
MPHA: 105832593
AEC: 105149600
ACEP: 105622350
PBAR: 105426911
VEM: 105568559
HST: 105184363
DQU: 106749327
CFO: 105248014
LHU: 105669682
PGC: 109855950
OBO: 105284082
PCF: 106784288
NVI: 100119267
CSOL: 105360197
MDL: 103575736
TCA: 659709
DPA: 109541778
ATD: 109598739
NVL: 108565052
BMOR: 101746534
BMAN: 114244560
PMAC: 106717133
PRAP: 110997563
HAW: 110377795
TNL: 113493937
PXY: 105381786
API: 100158868
DNX: 107171166
AGS: 114130037
RMD: 113553488
BTAB: 109029927
CLEC: 106662400
ZNE: 110838192
FCD: 110856238
PVM: 113818476
TUT: 107368943
DPTE: 113792907
PTEP: 107453367
CEL: CELE_T03G11.4(mans-3)
CBR: CBG14624
TSP: Tsp_04164
PCAN: 112575110
CRG: 105334865
MYI: 110447565
OBI: 106884360
EGL: EGR_00365
EPA: 110237771
ADF: 107335017
AMIL: 114946959
SPIS: 111332673
DGT: 114519075
HMG: 100198486
ATH: AT1G30000(MNS3)
ALY: 9326907
CRB: 17898338
RSZ: 108806417
THJ: 104812670
CPAP: 110807600
CIT: 102616435
TCC: 18596509
EGR: 104440790
VRA: 106775885
VAR: 108343148
VUN: 114187820
CCAJ: 109789197
CAM: 101515695
LJA: Lj6g3v1934090.1(Lj6g3v1934090.1)
ADU: 107468140
AIP: 107622562
LANG: 109345269
FVE: 101313190
RCN: 112164535
PPER: 18780386
PMUM: 103325119
PAVI: 110773985
PXB: 103951272
ZJU: 107413762
CSV: 101216212
CMO: 103484372
MCHA: 111015717
RCU: 8264122
JCU: 105633984
HBR: 110651587
POP: 7472574
PEU: 105118639
JRE: 108988627
VVI: 100243430
SLY: 101246787
SPEN: 107011307
SOT: 102583221
CANN: 107859093
NSY: 104249918
NTO: 104097810
NAU: 109216453
INI: 109192025
SIND: 105175706
OEU: 111402240
HAN: 110911812
LSV: 111880716
CCAV: 112526325
BVG: 104883805
SOE: 110794113
NNU: 104613507
OSA: 4338088
DOSA: Os05t0209100-01(Os05g0209100)
OBR: 102699961
BDI: 100820902
ATS: 109759492(LOC109759492)
SBI: 8055739
ZMA: 100282405(cl335_1)
SITA: 101773458
DCT: 110116511
PEQ: 110022272
AOF: 109828813
ATR: 18435237
PPP: 112287912
CRE: CHLREDRAFT_178232(AMD1)
VCN: VOLCADRAFT_119746(amd1)
APRO: F751_6268
MIS: MICPUN_112689(MANA)
SCE: YJR131W(MNS1)
ERC: Ecym_5170
KMX: KLMA_40509(MNS1)
NCS: NCAS_0J02160(NCAS0J02160)
NDI: NDAI_0J02870(NDAI0J02870)
TPF: TPHA_0L00320(TPHA0L00320)
TBL: TBLA_0B06140(TBLA0B06140)
TDL: TDEL_0D00600(TDEL0D00600)
KAF: KAFR_0E04290(KAFR0E04290)
PIC: PICST_84949(MNS1)
SPAA: SPAPADRAFT_57837(MNS1.1)
CAL: CAALFM_C103730CA(MNS1)
CTEN: CANTEDRAFT_110685(MNS1)
SLB: AWJ20_4611(MNS1)
NCR: NCU02778(gh47-1)
NTE: NEUTE1DRAFT74512(NEUTE1DRAFT_74512)
MGR: MGG_11408
SSCK: SPSK_05990
MAW: MAC_03073
MAJ: MAA_04109
CMT: CCM_08185
BFU: BCIN_01g06120(Bcmns1)
MBE: MBM_06132
ANG: ANI_1_1456164(An18g06220)
ABE: ARB_01040
TVE: TRV_02790
PTE: PTT_19626
ZTR: MYCGRDRAFT_45066(MgAMN9)
SPO: SPAC2E1P5.01c(mns1)
CNE: CNE04550
CNB: CNBE4550
TASA: A1Q1_06697
ABP: AGABI1DRAFT117691(AGABI1DRAFT_117691) AGABI1DRAFT130713(AGABI1DRAFT_130713) AGABI1DRAFT54540(AGABI1DRAFT_54540) AGABI1DRAFT70315(AGABI1DRAFT_70315)
ABV: AGABI2DRAFT176302(AGABI2DRAFT_176302) AGABI2DRAFT181939(AGABI2DRAFT_181939) AGABI2DRAFT206040(AGABI2DRAFT_206040) AGABI2DRAFT222683(AGABI2DRAFT_222683)
MRT: MRET_4039
SMIN: v1.2.009441.t1(symbB.v1.2.009441.t1) v1.2.009441.t2(symbB.v1.2.009441.t2) v1.2.025226.t1(symbB.v1.2.025226.t1) v1.2.032603.t1(symbB.v1.2.032603.t1)
FCY: FRACYDRAFT_261302(MNS2_1)
 » show all
Reference
1  [PMID:3049586]
  Authors
Jelinek-Kelly S, Herscovics A
  Title
Glycoprotein biosynthesis in Saccharomyces cerevisiae. Purification of the alpha-mannosidase which removes one specific mannose residue from Man9GlcNAc.
  Journal
J Biol Chem 263:14757-63 (1988)
Reference
2  [PMID:1822240]
  Authors
Ziegler FD, Trimble RB
  Title
Glycoprotein biosynthesis in yeast: purification and characterization of the endoplasmic reticulum Man9 processing alpha-mannosidase.
  Journal
Glycobiology 1:605-14 (1991)
DOI:10.1093/glycob/1.6.605
Reference
3  [PMID:10409699]
  Authors
Gonzalez DS, Karaveg K, Vandersall-Nairn AS, Lal A, Moremen KW
  Title
Identification, expression, and characterization of a cDNA encoding human endoplasmic reticulum mannosidase I, the enzyme that catalyzes the first mannose  trimming step in mammalian Asn-linked oligosaccharide biosynthesis.
  Journal
J Biol Chem 274:21375-86 (1999)
DOI:10.1074/jbc.274.30.21375
  Sequence
[hsa:11253]
Reference
4  [PMID:12090241]
  Authors
Herscovics A, Romero PA, Tremblay LO
  Title
The specificity of the yeast and human class I ER alpha 1,2-mannosidases involved in ER quality control is not as strict previously reported.
  Journal
Glycobiology 12:14G-15G (2002)
Reference
5  [PMID:18003979]
  Authors
Avezov E, Frenkel Z, Ehrlich M, Herscovics A, Lederkremer GZ
  Title
Endoplasmic reticulum (ER) mannosidase I is compartmentalized and required for N-glycan trimming to Man5-6GlcNAc2 in glycoprotein ER-associated degradation.
  Journal
Mol Biol Cell 19:216-25 (2008)
DOI:10.1091/mbc.e07-05-0505
  Sequence
[hsa:11253]
Reference
6  [PMID:20023195]
  Authors
Liebminger E, Huttner S, Vavra U, Fischl R, Schoberer J, Grass J, Blaukopf C, Seifert GJ, Altmann F, Mach L, Strasser R
  Title
Class I alpha-mannosidases are required for N-glycan processing and root development in Arabidopsis thaliana.
  Journal
Plant Cell 21:3850-67 (2009)
DOI:10.1105/tpc.109.072363
  Sequence
[ath:AT1G30000]
Reference
7  [PMID:19181540]
  Authors
Prien JM, Ashline DJ, Lapadula AJ, Zhang H, Reinhold VN
  Title
The high mannose glycans from bovine ribonuclease B isomer characterization by ion trap MS.
  Journal
J Am Soc Mass Spectrom 20:539-56 (2009)
DOI:10.1016/j.jasms.2008.11.012
Other DBs
ExplorEnz - The Enzyme Database: 3.2.1.209
IUBMB Enzyme Nomenclature: 3.2.1.209
ExPASy - ENZYME nomenclature database: 3.2.1.209
BRENDA, the Enzyme Database: 3.2.1.209
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DBGET integrated database retrieval system