KEGG   ENZYME: 6.2.1.65
Entry
EC 6.2.1.65                 Enzyme                                 

Name
salicylate---CoA ligase;
sdgA (gene name)
Class
Ligases;
Forming carbon-sulfur bonds;
Acid-thiol ligases
Sysname
salicylate:CoA ligase (AMP-forming)
Reaction(IUBMB)
ATP + salicylate + CoA = AMP + diphosphate + 2-hydroxybenzoyl-CoA (overall reaction) [RN:R12642];
(1a) ATP + salicylate = diphosphate + (2-hydroxybenzoyl)adenylate [RN:R12640];
(1b) (2-hydroxybenzoyl)adenylate + CoA = AMP + 2-hydroxybenzoyl-CoA [RN:R12641]
Reaction(KEGG)
Substrate
ATP [CPD:C00002];
salicylate [CPD:C00805];
CoA [CPD:C00010];
(2-hydroxybenzoyl)adenylate [CPD:C22233]
Product
AMP [CPD:C00020];
diphosphate [CPD:C00013];
2-hydroxybenzoyl-CoA [CPD:C20394];
(2-hydroxybenzoyl)adenylate [CPD:C22233]
Comment
The enzyme, characterized from the bacteria Thauera aromatica and Streptomyces sp. WA46, participates in a salicylate degradation pathway. It activates salicylate by its adenylation to (2-hydroxybenzoyl)adenylate, followed by the transfer of the activated compound to coenzyme A.
History
EC 6.2.1.65 created 2020
Orthology
K24408  salicylate---CoA ligase
Genes
RHQ: IM25_24395
NOW: GBF35_46440
AMQ: AMETH_3626(dhbE)
PDX: Psed_5064
Reference
1  [PMID:8661934]
  Authors
Bonting CF, Fuchs G
  Title
Anaerobic metabolism of 2-hydroxybenzoic acid (salicylic acid) by a denitrifying  bacterium.
  Journal
Arch Microbiol 165:402-8 (1996)
DOI:10.1007/s002030050344
Reference
2  [PMID:15006746]
  Authors
Ishiyama D, Vujaklija D, Davies J
  Title
Novel pathway of salicylate degradation by Streptomyces sp. strain WA46.
  Journal
Appl Environ Microbiol 70:1297-306 (2004)
DOI:10.1128/AEM.70.3.1297-1306.2004
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 6.2.1.65
IUBMB Enzyme Nomenclature: 6.2.1.65
ExPASy - ENZYME nomenclature database: 6.2.1.65
BRENDA, the Enzyme Database: 6.2.1.65
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