The enzyme biotinylates a biotin carboxyl-carrier protein that is part of an acetyl-CoA carboxylase complex, enabling its subsequent carboxylation by EC
6.3.4.14, biotin carboxylase. The carboxyl group is eventually transferred to acetyl-CoA by EC
2.1.3.15, acetyl-CoA carboxytransferase. In some organisms the carrier protein is part of EC
6.4.1.2, acetyl-CoA carboxylase.