KEGG   ENZYME: 6.5.1.8Help
Entry
EC 6.5.1.8                  Enzyme                                 

Name
3'-phosphate/5'-hydroxy nucleic acid ligase;
rtcB (gene name)
Class
Ligases;
Forming phosphoric-ester bonds;
Ligases that form phosphoric-ester bonds (only sub-subclass identified to date)
BRITE hierarchy
Sysname
poly(ribonucleotide)-3'-phosphate:5'-hydroxy-poly(ribonucleotide) ligase (GMP-forming)
Reaction(IUBMB)
(1) (ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + GTP = (ribonucleotide)n+m + GMP + diphosphate (overall reaction);
(1a) GTP + [RNA ligase]-L-histidine = [RNA ligase]-Ntau-(5'-guanosyl-phosphono)-L-histidine + diphosphate;
(1b) [RNA ligase]-Ntau-(5'-guanosyl-phosphono)-L-histidine + (ribonucleotide)n-3'-phosphate = (ribonucleotide)n-3'-(5'-diphosphoguanosine) + [RNA ligase]-L-histidine;
(1c) (ribonucleotide)n-3'-(5'-diphosphoguanosine) + 5'-hydroxy-(ribonucleotide)m = (ribonucleotide)n+m + GMP;
(2) (ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O = (ribonucleotide)n+m + GMP + diphosphate (overall reaction);
(2a) (ribonucleotide)n-2',3'-cyclophosphate + H2O = (ribonucleotide)n-3'-phosphate;
(2b) GTP + [RNA ligase]-L-histidine = [RNA ligase]-Ntau-(5'-guanosyl-phosphono)-L-histidine + diphosphate;
(2c) [RNA ligase]-Ntau-(5'-guanosyl-phosphono)-L-histidine + (ribonucleotide)n-3'-phosphate = (ribonucleotide)n-3'-(5'-diphosphoguanosine) + [RNA ligase]-L-histidine;
(2d) (ribonucleotide)n-3'-(5'-diphosphoguanosine) + 5'-hydroxy-(ribonucleotide)m = (ribonucleotide)n+m + GMP
Substrate
(ribonucleotide)n-3'-phosphate;
5'-hydroxy-(ribonucleotide)m;
GTP [CPD:C00044];
[RNA ligase]-L-histidine;
[RNA ligase]-Ntau-(5'-guanosyl-phosphono)-L-histidine;
(ribonucleotide)n-3'-(5'-diphosphoguanosine);
(ribonucleotide)n-2',3'-cyclophosphate;
H2O [CPD:C00001]
Product
(ribonucleotide)n+m [CPD:C00046];
GMP [CPD:C00144];
diphosphate [CPD:C00013];
[RNA ligase]-Ntau-(5'-guanosyl-phosphono)-L-histidine;
(ribonucleotide)n-3'-(5'-diphosphoguanosine);
[RNA ligase]-L-histidine;
(ribonucleotide)n-3'-phosphate
Comment
The enzyme is a GTP- and Mn2+-dependent 3'-5' nucleic acid ligase with the ability to join RNA with 3'-phosphate or 2',3'-cyclic-phosphate ends to RNA with 5'-hydroxy ends. It can also join DNA with 3'-phosphate ends to DNA with 5'-hydroxy ends, provided the DNA termini are unpaired [6]. The enzyme is found in members of all three kingdoms of life, and is essential in metazoa for the splicing of intron-containing tRNAs. The reaction follows a three-step mechanism with initial activation of the enzyme by GTP hydrolysis, forming a phosphoramide bond between the guanylate and a histidine residue. The guanylate group is transferred to the 3'-phosphate terminus of the substrate, forming the capped structure [DNA/RNA]-3'-(5'-diphosphoguanosine). When a suitable 5'-OH end is available, the enzyme catalyses an attack of the 5'-OH on the capped end to form a 3'-5' phosphodiester splice junction, releasing the guanylate. When acting on an RNA 2',3'-cyclic-phosphate, the enzyme catalyses an additional reaction, hydrolysing the cyclic phosphate to a 3'-phosphate [9]. The metazoan enzyme requires activating cofactors in order to achieve multiple turnover catalysis [8].
History
EC 6.5.1.8 created 2017
Orthology
K14415  tRNA-splicing ligase RtcB (3'-phosphate/5'-hydroxy nucleic acid ligase)
Genes
HSA: 51493(RTCB)
PTR: 458782(RTCB)
PPS: 100971358(RTCB)
GGO: 101144723(RTCB)
PON: 100439200(RTCB)
NLE: 100595915(RTCB)
MCC: 717258(RTCB)
MCF: 102144880(RTCB)
CSAB: 103223215(RTCB)
RRO: 104674734 104674737 104680252
RBB: 108535095(RTCB)
CJC: 100408961(RTCB)
MMU: 28088(Rtcb)
MCAL: 110303293(Rtcb)
MPAH: 110326155(Rtcb)
RNO: 362855(Rtcb)
CGE: 100753929(Rtcb)
NGI: 103742114(Rtcb)
HGL: 101717628(Rtcb)
CCAN: 109698924(Rtcb)
OCU: 100346526(RTCB)
TUP: 102492731(RTCB)
CFA: 474524(RTCB)
VVP: 112929061(RTCB)
AML: 100467421(RTCB)
UMR: 103676455(RTCB)
UAH: 113256024(RTCB)
ORO: 101385250(RTCB)
FCA: 101090197(RTCB)
PTG: 102966524(RTCB)
AJU: 106971932(RTCB)
BTA: 525106(RTCB)
BOM: 102286278(RTCB)
BIU: 109559482(RTCB)
BBUB: 102402412(RTCB)
PHD: 102318020(RTCB)
CHX: 102189284(RTCB)
OAS: 101118007(RTCB)
SSC: 733658(RTCB)
CFR: 102520545(RTCB)
CDK: 105101076(RTCB)
BACU: 102998172(RTCB)
LVE: 103075690(RTCB)
OOR: 101279974(RTCB)
DLE: 111187917(RTCB)
ECB: 100054153(RTCB)
EPZ: 103552701(RTCB)
EAI: 106838341(RTCB)
MYB: 102243411(RTCB)
MYD: 102768836(RTCB)
MNA: 107544989(RTCB)
HAI: 109382848(RTCB)
RSS: 109445775(RTCB)
DRO: 112321780(RTCB)
PALE: 102882315(RTCB)
LAV: 100675436(RTCB)
TMU: 101348587
MDO: 100016063(RTCB)
SHR: 100928215(RTCB)
PCW: 110205700(RTCB)
OAA: 100077731(RTCB)
GGA: 418064(RTCB)
MGP: 100539950(RTCB)
CJO: 107314485(RTCB)
APLA: 101795314(RTCB)
ACYG: 106035776(RTCB)
TGU: 100227339(RTCB)
LSR: 110483823(RTCB)
SCAN: 103813204(RTCB)
GFR: 102031778(RTCB)
FAB: 101819411(RTCB)
PHI: 102099960(RTCB)
PMAJ: 107204353(RTCB)
CCAE: 111931292(RTCB)
CCW: 104696258(RTCB)
ETL: 114058867(RTCB)
FPG: 101916493(RTCB)
FCH: 114014219(RTCB)
CLV: 106145813(RTCB)
EGZ: 104134155(RTCB)
NNI: 104020743(RTCB)
ACUN: 113482139(RTCB)
AAM: 106488831(RTCB)
ASN: 102379448(RTCB)
AMJ: 102577238(RTCB)
PSS: 102463710(RTCB)
CPIC: 101948221(RTCB)
ACS: 100554682(rtcb)
PVT: 110084369(RTCB)
PBI: 103063098(RTCB)
PMUR: 107284725(RTCB)
GJA: 107124178(RTCB)
XLA: 108712553 779280(rtcb.L)
XTR: 595066(rtcb)
NPR: 108789951(RTCB)
DRE: 406376(rtcb)
SRX: 107707948 107710072(rtcb)
SANH: 107662217
SGH: 107568717(rtcb)
CCAR: 109113272
IPU: 108274978(rtcb)
PHYP: 113529486(rtcb)
AMEX: 103036305(rtcb)
EEE: 113573675(rtcb)
TRU: 101070621(rtcb)
LCO: 104931389(rtcb)
MZE: 101478440(rtcb)
OLA: 101164466(rtcb)
XMA: 102228137(rtcb)
XCO: 114161262(rtcb)
PRET: 103459793(rtcb)
NFU: 107387696(rtcb)
KMR: 108229103(rtcb)
CSEM: 103382483(rtcb)
LCF: 108882416(rtcb)
SDU: 111229477(rtcb)
HCQ: 109532601(rtcb)
BPEC: 110161894(rtcb)
MALB: 109967659(rtcb)
SASA: 106593674(rtcb) 106594583
OTW: 112238853(rtcb)
SALP: 112079181(rtcb)
ELS: 105026520(rtcb)
PKI: 111846996(rtcb)
LCM: 102358373(RTCB)
CMK: 103181817(rtcb)
CIN: 100176177
SPU: 588300
APLC: 110982862
SKO: 100377188
DME: Dmel_CG9987(RtcB)
DER: 6549653
DPE: 6597959
DSI: Dsimw501_GD24205(Dsim_GD24205)
DWI: 6641666
DAZ: 108610312
DHE: 111592590
AAG: 5577915
AME: 409653
BIM: 100745965
BTER: 100647213
SOC: 105203762
MPHA: 105828557
AEC: 105143294
ACEP: 105623347
PBAR: 105432766
HST: 105184601
DQU: 106746563
CFO: 105250110
LHU: 105678651
PGC: 109855294
OBO: 105278260
PCF: 106786206
NVI: 100123449
MDL: 103579776
TCA: 658169
DPA: 109545488
NVL: 108561811
BMOR: 101739631
PMAC: 106710710
PRAP: 110992677
HAW: 110369919
TNL: 113508025
PXY: 105391195
DNX: 107171119
AGS: 114122803
CLEC: 106661619
ZNE: 110831184
FCD: 110862618
TUT: 107367363
DPTE: 113793956
CEL: CELE_F16A11.2(rtcb-1)
CBR: CBG22190
BMY: Bm1_49220
PCAN: 112571587
MYI: 110462262
OBI: 106867593
EGL: EGR_00905
EPA: 110236795
HMG: 100200632
AQU: 100635515
MNG: MNEG_5458
APRO: F751_2036
DFA: DFA_10051
EHI: EHI_169260(177.t00019) EHI_184560(391.t00003)
PYO: PY17X_0943700(PY03776)
PCB: PCHAS_090310(PC000172.03.0)
TAN: TA10620
TPV: TP04_0661
BBO: BBOV_III007770(17.m07681)
SMIN: v1.2.002119.t1(symbB.v1.2.002119.t1) v1.2.007748.t1(symbB.v1.2.007748.t1) v1.2.021296.t1(symbB.v1.2.021296.t1)
ECO: b3421(rtcB)
ECJ: JW3384(rtcB)
ECD: ECDH10B_3595(rtcB)
EBW: BWG_3114(rtcB)
ECOK: ECMDS42_2866(rtcB)
ECE: Z4779(rtcB)
ECS: ECs4264
ECF: ECH74115_4729(rtcB)
ETW: ECSP_4373(rtcB)
EOJ: ECO26_4508(rtcB)
EOI: ECO111_4229(rtcB)
ECOO: ECRM13514_4370(rtcB)
ECOH: ECRM13516_4167(rtcB)
ECG: E2348C_3665(rtcB)
EOK: G2583_4120(rtcB)
ESO: O3O_23690
ESM: O3M_02005
ESL: O3K_01960
ECW: EcE24377A_3896(rtcB)
ELH: ETEC_3670
ECC: c4198(rtcB)
ECP: ECP_3506
ECI: UTI89_C3921(rtcB)
ECV: APECO1_3046(rtcB)
ECX: EcHS_A3618(rtcB)
ECM: EcSMS35_3701(rtcB)
ECY: ECSE_3687
ECR: ECIAI1_3564(rtcB)
ECQ: ECED1_4081(rtcB)
ECK: EC55989_3828(rtcB)
EOC: CE10_0717 CE10_3941(rtcB)
EUM: ECUMN_3879(rtcB)
ECZ: ECS88_3809(rtcB)
ELO: EC042_3681(rtcB)
EBR: ECB_03272(rtcB)
EBD: ECBD_0321
EAB: ECABU_c38410(rtcB)
EDJ: ECDH1ME8569_3299(rtcB)
EIH: ECOK1_3836(rtcB)
ELW: ECW_m3678(rtcB)
ELL: WFL_17970
ELC: i14_3869(rtcB)
ELD: i02_3869(rtcB)
EBL: ECD_03272(rtcB)
EBE: B21_03224(rtcB)
ELF: LF82_2042(rtcB)
ECOI: ECOPMV1_03727(rtcB_2)
EFE: EFER_3389(rtcB)
EAL: EAKF1_ch2543(rtcB)
STM: STM3519(rtcB)
SEO: STM14_4238(rtcB)
SEY: SL1344_3486(rtcB)
SEJ: STMUK_3505(rtcB)
SEB: STM474_3687(rtcB)
SENR: STMDT2_34061(rtcB)
SEND: DT104_35031(rtcB)
SENI: CY43_18290
SEI: SPC_3588(rtcB)
SEC: SCH_3450(rtcB)
SEH: SeHA_C3827(rtcB)
SHB: SU5_03994
SENS: Q786_17175
SED: SeD_A3890(rtcB)
SEG: SG3919(rtcB)
SEL: SPUL_4060
SET: SEN3345(rtcB)
SENA: AU38_17065
SENO: AU37_17255
SENV: AU39_17260
SENQ: AU40_19230
SENL: IY59_17690
SEEP: I137_19410
SENE: IA1_17065
SFL: SF3443(rtcB)
SFX: S4321(rtcB)
SFV: SFV_3428(rtcB)
SFE: SFxv_3759
SFN: SFy_4899
SFS: SFyv_4972
SFT: NCTC1_03724(rtcB_2)
SSN: SSON_3552(rtcB)
SBO: SBO_3409(rtcB)
SBC: SbBS512_E3801(rtcB)
ESA: ESA_00065
CSK: ES15_0387(rtcB)
CTU: CTU_37920(rtcB)
EAE: EAE_08330
EAR: CCG32330
CRO: ROD_37281(rtcB)
CAMA: F384_22205
METY: MRY16398_53340(rtcB)
PLU: plu4307(rtcB)
PAY: PAU_00438
PHAU: PH4a_11200
XBO: XBJ1_0417(rtcB) XBJ1_0478
XBV: XBW1_4276 XBW1_4362(rtcB)
XNE: XNC1_0541
XNM: XNC2_0530
XDO: XDD1_0683
PSI: S70_08035
PSX: DR96_1573(rtcB)
PRG: RB151_005160(rtcB_1)
PHEI: NCTC12003_00520(rtcB_1)
MMK: MU9_137
XCC: XCC3883
XCB: XC_3970
XCP: XCR_0396
XCV: XCV4058
XAX: XACM_3836
XAC: XAC3965
XPH: XppCFBP6546_14695(XppCFBP6546P_14695)
SML: Smlt4533
SMT: Smal_3893
SMZ: SMD_4076(rtcB)
LAB: LA76x_0969(rtcB) LA76x_4435(rtcB)
LAQ: GLA29479_2186(rtcB) GLA29479_2482(rtcB)
LCP: LC55x_0729(rtcB) LC55x_4477(rtcB)
LGU: LG3211_0665(rtcB) LG3211_4287(rtcB)
LEM: LEN_0578 LEN_0868(rtcB)
DKO: I596_263
VNI: VIBNI_A1840(rtcB)
PAE: PA4583
PAEV: N297_4734
PAEI: N296_4734
PAEP: PA1S_24845
PAEM: U769_25105
PAEC: M802_4732
PAEO: M801_4599
PMY: Pmen_1193
PCQ: PcP3B5_10160(rtcB)
PPT: PPS_0526
PPUH: B479_03135
PPUN: PP4_05660(rtcB)
PPUD: DW66_0540
PMON: X969_01075
PMOT: X970_01065
PFO: Pfl01_1946(rtcB)
PMAN: OU5_5751
PEN: PSEEN0615
PKC: PKB_4819(rtcB)
PSES: PSCI_2979
PSEM: TO66_11495
PSEC: CCOS191_4789(rtcB)
PSOS: POS17_2291 POS17_3754(PFL_3584)
PSET: THL1_956
PSIL: PMA3_11010
ACX: Achr_5350
PSYC: DABAL43B_0978(rtcB3)
PSYA: AOT82_801
ABY: ABAYE0642
ABN: AB57_3342
ABX: ABK1_3139
ABH: M3Q_3325
ABAU: IX87_11075
ABAA: IX88_17380
SDE: Sde_0273
MICC: AUP74_02854(rtcB_1) AUP74_03320(rtcB_2)
CBD: CBUD_1265
CBG: CbuG_0839
CBC: CbuK_1035
LPN: lpg2201
LPH: LPV_2454
LPO: LPO_2270
LPM: LP6_2228
LPF: lpl2125
LPP: lpp2151
LPC: LPC_1665
LPA: lpa_03159
LPE: lp12_2193
LLO: LLO_0715
LFA: LFA_2009(rtcB)
LHA: LHA_2113
LOK: Loa_01469
LCD: clem_04745(rtcB)
LLG: 44548918_00874(rtcB)
TMC: LMI_3092
MCA: MCA0195
TIG: THII_0482
NOC: Noc_2009
NWA: Nwat_1815
TEE: Tel_01470
AEH: Mlg_1728
HHA: Hhal_2355
TGR: Tgr7_1950
TNI: TVNIR_1860(rtcB_[H])
GAI: IMCC3135_09675(rtcB)
HBE: BEI_0946
AXE: P40_01815
SLIM: SCL_0752
SVA: SVA_2976
CVI: CV_1037(rtcb) CV_1425
PSE: NH8B_1362
RSO: RSc0224(rtcB)
RSN: RSPO_c03176(rtcB)
RSM: CMR15_30725(rtcB)
RSE: F504_241
REH: H16_B0555(h16_B0555)
CNC: CNE_2c05030(rtcB)
BTQ: BTQ_4598
BTJ: BTJ_5581
BTZ: BTL_4048
BTV: BTHA_3833
BTHE: BTN_3619
BTHM: BTRA_4318
BTHA: DR62_3653
BTHL: BG87_4055
BOK: DM82_5257
BOC: BG90_5352
BCN: Bcen_4671
BCJ: BCAM0726
BCEN: DM39_4438
BCEW: DM40_3717
BCEO: I35_4625
BAM: Bamb_5432
BCT: GEM_5070
BCED: DM42_4362
BDL: AK34_4767
BCON: NL30_22245
BTEI: WS51_27875
BSEM: WJ12_23630
BPSL: WS57_10340
BSTG: WT74_22460
BGO: BM43_3167
HYF: DTO96_101865(rtcB)
BAV: BAV3408(rtcB)
AMIM: MIM_c10490(rtcB)
RFR: Rfer_3852
POL: Bpro_1473
PNA: Pnap_0498
AAA: Acav_0015
VPD: VAPA_1c45930(rtcB)
JAG: GJA_256(rtcB)
CARE: LT85_0053(rtcB)
RDP: RD2015_46
NET: Neut_0880
TBD: Tbd_2359
DAR: Daro_1873
AZO: azo0079(rtcB)
AZA: AZKH_1503 AZKH_2570(rtcB)
AOA: dqs_0089
GSU: GSU1684
GME: Gmet_1619
GUR: Gura_1393
GBM: Gbem_0989
GEO: Geob_2321
GEM: GM21_3271
GEB: GM18_3507
PCA: Pcar_0019 Pcar_2835(rtcB)
PPD: Ppro_2142
DEU: DBW_3579
DVU: DVU0853
DVL: Dvul_2130
DVM: DvMF_2898
DDE: Dde_2089
DMA: DMR_25460
DHY: DESAM_22337(rtcB)
DGG: DGI_0981(rtcB)
DAS: Daes_1800
DRT: Dret_0333
DOL: Dole_2571
DML: Dmul_39100(rtcB)
DAL: Dalk_3935
DAT: HRM2_05280(rtcB)
ADE: Adeh_0946
HOH: Hoch_0235
SAT: SYN_02046
SFU: Sfum_2726
DBR: Deba_0260
BBA: Bd2854
BBAT: Bdt_2795
BBAC: EP01_10385
BRA: BRADO2887
BBT: BBta_5290
AOL: S58_47240
MNO: Mnod_4254
SSAN: NX02_17430
SSY: SLG_10960
SMIC: SmB9_19760(rtcB)
ANH: A6F65_00507(rtcB)
MAGX: XM1_0077
TMO: TMO_c0758
MAGQ: MGMAQ_3163
BHA: BH1789
BCQ: BCQ_2815
BCL: ABC1919
PPO: PPM_0868(hint) PPM_3745(rtcB)
PSAB: PSAB_03550
PRI: PRIO_1542
COHN: KCTCHS21_47760(rtcB)
CAC: CA_C3382
CAE: SMB_G3419
CAY: CEA_G3385
CPAT: CLPA_c04020(rtcB)
CPAE: CPAST_c04020(rtcB)
CSO: CLS_28730
STH: STH1327
SLP: Slip_2150
PTH: PTH_1582(RtcB)
DAU: Daud_0477
TMR: Tmar_2086
BPRM: CL3_11340
TTE: TTE0709(RtcB)
TIT: Thit_0712
TKI: TKV_c06970(rtcB)
CHY: CHY_0177
ADG: Adeg_1757
CSC: Csac_1247
ATE: Athe_2183
TOC: Toce_1735
LPIL: LIP_0340
MTU: Rv2631
MTV: RVBD_2631
MTC: MT2707
MRA: MRA_2659
MTUR: CFBS_2784
MTD: UDA_2631
MTUE: J114_14075
MTUH: I917_18570
MTUL: TBHG_02569
MTUT: HKBT1_2775
MTUU: HKBT2_2778
MBB: BCG_2658
MBT: JTY_2652
MBX: BCGT_2477
MAF: MAF_26500
MMIC: RN08_2913
MHAD: B586_06115
MFT: XA26_50760(rtcB)
MPHL: MPHLCCUG_01543(rtcB)
MTHN: 4412656_01156(rtcB)
MHAS: MHAS_00467(rtcB)
MABB: MASS_3426
CGL: NCgl2899(Cgl3001)
CGB: cg3329
CGU: WA5_2899
CGT: cgR_2890
CGM: cgp_3329
CGJ: AR0_14540
CEF: CE2835
CDI: DIP2025
CDIP: ERS451417_00438(rtcB)
CUR: cu1387
CGY: CGLY_12595(rtcB)
CSP: WM42_1082
CAQU: CAQU_11930
CAMG: CAMM_05485
NFA: NFA_13690
NFR: ERS450000_02738(rtcB)
NSR: NS506_06845(rtcB)
REY: O5Y_11565
ROP: ROP_68550
REQ: REQ_13440
RFA: A3L23_00586(rtcB)
RHS: A3Q41_02769(rtcB)
GOR: KTR9_3108
GRU: GCWB2_17505(rtcB)
GOM: D7316_02205(rtcB)
TPR: Tpau_0543
SCO: SCO3299(SCE15.16c)
SALB: XNR_3545
SGR: SGR_4185
SGB: WQO_14680
SCT: SCAT_3437(rtcB) SCAT_5020
SFA: Sfla_3574
SBH: SBI_06030
SHY: SHJG_5623
SVE: SVEN_3171
SDV: BN159_3960(rtcB)
SALS: SLNWT_2798
STRP: F750_3148(rtcB)
SFI: SFUL_2975
SALU: DC74_3951
SALL: SAZ_20890
SLV: SLIV_21295(rtcB)
SGU: SGLAU_02850(rtcB1) SGLAU_19240(rtcB2)
STRE: GZL_04947
SLD: T261_4518
STRM: M444_20030
SAMB: SAM23877_4256(rtcB)
SPRI: SPRI_3335
SRW: TUE45_05006(rtcB)
SLE: sle_06760(sle_06760) sle_31340(sle_31340)
SRN: A4G23_02879(rtcB_1) A4G23_03205(rtcB_2)
STRD: NI25_16645
SMAL: SMALA_4317
SALJ: SMD11_3719
SLX: SLAV_21630(rtcB)
SFK: KY5_4574
KSK: KSE_54190
MTS: MTES_0446
ART: Arth_3119
ACH: Achl_4478
RSA: RSal33209_0887(rtcB)
JDE: Jden_1934
CFL: Cfla_0012
CFI: Celf_0498
SERJ: SGUI_2324
BLIN: BLSMQ_2835
PFRE: RM25_2257
PPC: HMPREF9154_1687(rtcB)
ACIJ: JS278_00105(rtcB)
MPH: MLP_03420
KFL: Kfla_6625
PSIM: KR76_06090
MGG: MPLG2_1348(rtcB)
TFU: Tfu_1357
NDA: Ndas_1411
NAL: B005_4010
FAL: FRAAL4283
NML: Namu_1716
GOB: Gobs_3677
MMAR: MODMU_4048
KRA: Krad_1410
SEN: SACE_3596
SACC: EYD13_12925(rtcB)
AMD: AMED_8879
AMN: RAM_45555
AMM: AMES_8744
AMZ: B737_8745
AOI: AORI_7651
AJA: AJAP_39890(rtcB)
PSEE: FRP1_15925
AMI: Amir_1475
SESP: BN6_17540(rtcB)
KAL: KALB_6876
ACTI: UA75_27355
ACAD: UA74_26765
AHG: AHOG_24665(rtcB)
ALO: CRK60868
SAQ: Sare_2548
MIL: ML5_5008
ASE: ACPL_2722(rtcB)
ACTS: ACWT_2824
CAI: Caci_2449
SNA: Snas_4449
TBI: Tbis_0835
CYA: CYA_2537
PSER: ABRG53_1080(rtcB)
AMR: AM1_4754
MAR: MAE_13610
MPK: VL20_1823
CYL: AA637_02110(rtcB)
CYT: cce_2761
GLJ: GKIL_4162(rtcB)
ANA: all3526
AVA: Ava_3418
CALH: IJ00_12925
NSP: BMF81_01032(rtcB)
CTHE: Chro_3580
DET: DET0823
DEH: cbdbA801
DEV: DhcVS_727
DMC: btf_744
DMD: dcmb_790
DMG: GY50_0737
RCA: Rcas_0873
CAU: Caur_0538
CAG: Cagg_1306
TRO: trd_A0625
STI: Sthe_3251
ATM: ANT_11350
ABAT: CFX1CAM_1516(rtcB)
PBF: CFX0092_A2977(rtcB)
DRA: DR_0430
TTH: TT_C1426(rtcB)
TTJ: TTHA1785
TAQ: TO73_2168
TTR: Tter_0374
PNL: PNK_2266(rtcB)
PUV: PUV_02780 PUV_02890(rtcB)
WCH: wcw_0561
MIN: Minf_0304(rtcB)
RBA: RB5774 RB9360(rtcB)
PSL: Psta_0360
PIR: VN12_02940(rtcB)
PLS: VT03_20620(rtcB_1) VT03_28500(rtcB_2)
PLH: VT85_08960(rtcB)
FMR: Fuma_00756(rtcB)
TTF: THTE_4461
GES: VT84_20705(rtcB_1) VT84_23780(rtcB_2)
IPA: Isop_1031
SACI: Sinac_5138
PBOR: BSF38_04344(rtcB)
PBAS: SMSP2_00402(rtcB)
ABAC: LuPra_00138(rtcB)
EPO: Epro_1088
GBA: J421_5518
BTH: BT_1455
BTHO: Btheta7330_01760(rtcB)
BFR: BF2711
BOA: Bovatus_00921(rtcB_1) Bovatus_01503(rtcB_2)
BCEL: BcellWH2_01238(rtcB_1) BcellWH2_04175(rtcB_2)
SRU: SRU_2493
SRM: SRM_02709
RMR: Rmar_0504
SGN: SGRA_3976
PHE: Phep_0086
SMIZ: 4412673_01206(rtcB_1) 4412673_01351(rtcB_2)
MUC: MuYL_3576
MGOT: MgSA37_00547(rtcB_1)
CHU: CHU_2904
DFE: Dfer_3725
SLI: Slin_1492
FAE: FAES_3385
HSW: Hsw_1616
CHE: CAHE_0550(rtcB)
FJG: BB050_02854(rtcB_1) BB050_04279(rtcB_2)
FBR: FBFL15_1051(rtcB)
FIN: KQS_11450(rtcB)
MLT: VC82_600
EAO: BD94_3903
ELB: VO54_02794(rtcB_2)
CHZ: CHSO_4773(rtcB)
WIN: WPG_1501
AALG: AREALGSMS7_03981(rtcB)
KOS: KORDIASMS9_00982(rtcB)
CTE: CT0601
CPC: Cpar_0618
CTS: Ctha_1975
IAL: IALB_1250
MRO: MROS_0532
CPRV: CYPRO_3003
AAE: aq_032
HTH: HTH_1018
TAL: Thal_0159
TTK: TST_1313(rtcB)
TMA: TM1357
TMW: THMA_1382
TMQ: THMB_1382
TMX: THMC_1382
TPT: Tpet_1426
TRQ: TRQ2_1472
TNA: CTN_1234
TNP: Tnap_1446
TLE: Tlet_0926
TME: Tmel_0875
TAF: THA_1132
THER: Y592_05195
FNO: Fnod_0681
MARN: LN42_07005
KOL: Kole_1045
CEX: CSE_07090
CABY: Cabys_2003(rtcB)
DTU: Dtur_0127
NDE: NIDE2519
NMV: NITMOv2_3246(rtcB)
NIO: NITINOP_0749(rtcB)
NJA: NSJP_3890(rtcB)
CTHI: THC_0235
BANA: BARAN1_0885(rtcB)
MOX: DAMO_1577
MJA: MJ_0682
MMP: MMP1392
MMD: GYY_07790
MAE: Maeo_0134
MVO: Mvol_0961
MTH: MTH_1597
METC: MTCT_1456
MWO: MWSIV6_0141(rtcB)
METE: tca_01546(rtcB)
MST: Msp_0641
MSI: Msm_0666
MRU: mru_0426
MEB: Abm4_0216
MMIL: sm9_0302(rtcB)
MEYE: TL18_01295
MOL: YLM1_0096
METH: MBMB1_1440(rtcB)
MFC: BRM9_0668
MFI: DSM1535_0639(rtcB)
MCUB: MCBB_1701(rtcB)
MFV: Mfer_0490
MKA: MK1682(rtcB)
AFU: AF_0862
FPL: Ferp_1294
GAC: GACE_0692
GAH: GAH_02018
TAC: Ta0279
TVO: TVG1391663(TVG1391663)
PTO: PTO0605
FAI: FAD_0098
CDIV: CPM_1733
MEAR: Mpt1_c01880(rtcB)
MARC: AR505_1406
ABI: Aboo_0966
PHO: PH1602(PH1602)
PAB: PAB0383
PFU: PF1615
PFI: PFC_10530
PYN: PNA2_0188
PYS: Py04_1491
TKO: TK0358
TON: TON_0714
TGA: TGAM_1505
TSI: TSIB_1552
THE: GQS_01315
THA: TAM4_1534
THM: CL1_1978
TLT: OCC_11789
THS: TES1_0931
TNU: BD01_1004
TEU: TEU_10910
PPAC: PAP_02800
MAC: MA_0266
MBAR: MSBR2_2031
MBAK: MSBR3_2141
MMA: MM_1538
MMAC: MSMAC_3149
METM: MSMTP_3027
MTHE: MSTHC_1260
MTHR: MSTHT_2025
MHOR: MSHOH_3860
MBU: Mbur_2207
MMET: MCMEM_0383
MMH: Mmah_0438
MPY: Mpsy_2320
MTP: Mthe_1374
MCJ: MCON_2082
MHI: Mhar_0588
MHU: Mhun_3181
MLA: Mlab_0129
MEMA: MMAB1_2517(rtcB) MMAB1_2518
MPI: Mpet_1701
MBN: Mboo_1792
MPL: Mpal_1018
MPD: MCP_2028
MEZ: Mtc_2035
RCI: LRC484
HAL: VNG_1454C
HSL: OE_3085R(rtcB)
HHB: Hhub_2827(rtcB)
HALH: HTSR_0561
HHSR: HSR6_0548
NPH: NP_1934A(rtcB2) NP_2178A(rtcB1)
NMO: Nmlp_2687(rtcB1)
HUT: Huta_2758
HTI: HTIA_2505
HVO: HVO_2712(rtcB)
HME: HFX_0513(rtcB_1) HFX_2723(rtcB)
HLA: Hlac_2024
HTU: Htur_2560
NMG: Nmag_0414(rtcB1)
NAT: NJ7G_1087
SALI: L593_06610
ACJ: ACAM_1104
SMR: Smar_0985
IHO: Igni_0393
IIS: EYM_00515
DKA: DKAM_1210
TAG: Tagg_0797
IAG: Igag_0136
HBU: Hbut_0518
STO: STK_12920
SSO: SSO0966
SOL: Ssol_1945
SSOA: SULA_1977
SSOL: SULB_1978
SSOF: SULC_1976
SAI: Saci_1317
SID: M164_1241
SII: LD85_1369
SIH: SiH_1202
SIR: SiRe_1120
SIC: SiL_1117
MSE: Msed_1752
MCN: Mcup_0477
AHO: Ahos_1417
PAI: PAE0998
PIS: Pisl_1219
PCL: Pcal_0141
PAS: Pars_0172
PYR: P186_2427
POG: Pogu_2307
TNE: Tneu_0157
CMA: Cmaq_1864
TUZ: TUZN_0719
TTN: TTX_1033
VDI: Vdis_2242
VMO: VMUT_0652
TPE: Tpen_0269
ACIA: SE86_05330
NMR: Nmar_1453
NID: NPIRD3C_2113(rtcB)
NIN: NADRNF5_0431(rtcB)
NCT: NMSP_0297(rtcB)
NEV: NTE_02933
TAA: NMY3_00808(rtcB)
NFN: NFRAN_1800(rtcB)
NCV: NCAV_0536(rtcB)
NBV: T478_0330
NDV: NDEV_1737(rtcB)
NEQ: NEQ078
NAA: Nps_02110
MARH: Mia14_0602
KCR: Kcr_1407
BARC: AOA65_1209(rtcB)
BARB: AOA66_0564(rtcB_1)
 » show all
Taxonomy
Reference
1  [PMID:21757685]
  Authors
Tanaka N, Meineke B, Shuman S
  Title
RtcB, a novel RNA ligase, can catalyze tRNA splicing and HAC1 mRNA splicing in vivo.
  Journal
J Biol Chem 286:30253-7 (2011)
DOI:10.1074/jbc.C111.274597
  Sequence
[eco:b3421]
Reference
2  [PMID:21224389]
  Authors
Tanaka N, Shuman S
  Title
RtcB is the RNA ligase component of an Escherichia coli RNA repair operon.
  Journal
J Biol Chem 286:7727-31 (2011)
DOI:10.1074/jbc.C111.219022
  Sequence
[eco:b3421]
Reference
3  [PMID:22045815]
  Authors
Tanaka N, Chakravarty AK, Maughan B, Shuman S
  Title
Novel mechanism of RNA repair by RtcB via sequential 2',3'-cyclic phosphodiesterase and 3'-Phosphate/5'-hydroxyl ligation reactions.
  Journal
J Biol Chem 286:43134-43 (2011)
DOI:10.1074/jbc.M111.302133
Reference
4  [PMID:22320833]
  Authors
Desai KK, Raines RT
  Title
tRNA ligase catalyzes the GTP-dependent ligation of RNA with 3'-phosphate and 5'-hydroxyl termini.
  Journal
Biochemistry 51:1333-5 (2012)
DOI:10.1021/bi201921a
  Sequence
[pho:PH1602]
Reference
5  [PMID:22474365]
  Authors
Chakravarty AK, Subbotin R, Chait BT, Shuman S
  Title
RNA ligase RtcB splices 3'-phosphate and 5'-OH ends via covalent RtcB-(histidinyl)-GMP and polynucleotide-(3')pp(5')G intermediates.
  Journal
Proc Natl Acad Sci U S A 109:6072-7 (2012)
DOI:10.1073/pnas.1201207109
Reference
6  [PMID:22730297]
  Authors
Chakravarty AK, Shuman S
  Title
The sequential 2',3'-cyclic phosphodiesterase and 3'-phosphate/5'-OH ligation steps of the RtcB RNA splicing pathway are GTP-dependent.
  Journal
Nucleic Acids Res 40:8558-67 (2012)
DOI:10.1093/nar/gks558
Reference
7  [PMID:24218597]
  Authors
Das U, Chakravarty AK, Remus BS, Shuman S
  Title
Rewriting the rules for end joining via enzymatic splicing of DNA 3'-PO4 and 5'-OH ends.
  Journal
Proc Natl Acad Sci U S A 110:20437-42 (2013)
DOI:10.1073/pnas.1314289110
Reference
8  [PMID:26385509]
  Authors
Desai KK, Beltrame AL, Raines RT
  Title
Coevolution of RtcB and Archease created a multiple-turnover RNA ligase.
  Journal
RNA 21:1866-72 (2015)
DOI:10.1261/rna.052639.115
Reference
9  [PMID:26858100]
  Authors
Maughan WP, Shuman S
  Title
Distinct Contributions of Enzymic Functional Groups to the 2',3'-Cyclic Phosphodiesterase, 3'-Phosphate Guanylylation, and 3'-ppG/5'-OH Ligation Steps of the Escherichia coli RtcB Nucleic Acid Splicing Pathway.
  Journal
J Bacteriol 198:1294-304 (2016)
DOI:10.1128/JB.00913-15
Other DBs
ExplorEnz - The Enzyme Database: 6.5.1.8
IUBMB Enzyme Nomenclature: 6.5.1.8
ExPASy - ENZYME nomenclature database: 6.5.1.8
BRENDA, the Enzyme Database: 6.5.1.8
LinkDB All DBs

DBGET integrated database retrieval system