Entry
Name
dihydroflavonol 4-reductase;
dihydrokaempferol 4-reductase;
dihydromyricetin reductase;
NADPH-dihydromyricetin reductase;
dihydroquercetin reductase;
DFR (gene name);
cis-3,4-leucopelargonidin:NADP+ 4-oxidoreductase;
dihydroflavanol 4-reductase (incorrect)
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
(2R,3S,4S)-leucoanthocyanidin:NADP+ 4-oxidoreductase
Reaction(IUBMB)
a (2R,3S,4S)-leucoanthocyanidin + NADP+ = a (2R,3R)-dihydroflavonol + NADPH + H+ [RN:
R11500 ]
Reaction(KEGG)
Substrate
(2R,3S,4S)-leucoanthocyanidin [CPD:
C21418 ];
NADP+ [CPD:
C00006 ]
Product
Comment
This plant enzyme, involved in the biosynthesis of anthocyanidins, is known to act on (+)-dihydrokaempferol, (+)-taxifolin, and (+)-dihydromyricetin, although some enzymes may act only on a subset of these compounds. Each dihydroflavonol is reduced to the corresponding cis-flavan-3,4-diol. NAD+ can act instead of NADP+, but more slowly.
History
EC 1.1.1.219 created 1989, modified 2016
Pathway
ec01110 Biosynthesis of secondary metabolites
Orthology
K00091 dihydroflavonol-4-reductase
K13082 bifunctional dihydroflavonol 4-reductase/flavanone 4-reductase
Genes
CSAT : 104725195 104760679 104771418
GHI : 107905441 107935085 107938889 107957957
HSYR : 120114676 120167561 120169034 120216676
DZI : 111312736 111315336 111315339
GMX : 100778253 548087(DFR1) 732626(DFR2)
GSJ : 114384244 114391094 114392892
PVU : 137816395 137816396 137830495
VRA : 106763833 106764122 106770756 106770772 106771250
VAR : 108324448 108324922 108336257 108336367
VUN : 114191920 114193233 114193234 114193708
VUM : 124830594 124830645 124830655
CCAJ : 109811985 109811998
APRC : 113845972 113846034
TPRA : 123886567 123886574
PSAT : 127093431 127093432
VVO : 131596616 131596617 131596618
LJA : 130717253 130717254 130719218 130720661 130720703 130723518
PCIN : 129303088 129303142 129306003 129306209
AANS : 126784953 126784968
MSYL : 126604732 126631239
JCU : 105633822 105633823 105633824 110008657
HBR : 110638079 110638080 110638081 110655218 110655234
MESC : 110605890 110615791 110615792
PALZ : 118052800 118062127
CILL : 122316519 122316520
CAVE : 132190022 132190424
AGLU : 133870740 133872009
TWL : 119995119 120008961 120012083
VVI : 100233141(DFR) 100259090 100267635
VRI : 117905400 117906273 117906599
SOT : 102577487 102587107(DFR)
NTA : 107797232(Dfr2) 107803097(Dfr1)
INI : 109177611(DFR-A) 109177619(DFR-B) 109177620(DFR-C)
ITR : 115997971 115999112 115999903
SSPL : 121768372 121769161
SHIS : 125214503 125214539
DCR : 108216819 108220625 108222116 108222765 108222766 108224331 108224780
CSIN : 114263316 114299086 114299087 114306385
AEW : 130758800 130783480 130784840
CQI : 110702165 110702205 110724547 110738821
NNU : 104589331 104589332 104590621 104601829
MING : 122070773 122070775 122075392 122076687 122086119 122086459 122094122
TSS : 122660095 122661531 122662474 122666599 122666608
PSOM : 113333459 113345760
TAES : 100125685 123070190 123078633
SBI : 8058202 8058203 8075972 8076097
MFLO : 136452553 136458558 136458559 136531928 136531932
PVIR : 120676859 120707535
NCOL : 116247360 116268364
ATR : 18422615 18422618 18422620
CJF : 131029863 131033341 131035160 131047830 131052622 131064246 131069342 131069348 131069366 131069367 131069368 131072065 131072067 131072068 131072083 131072084 131072085 131072086 131072087 131072089 131072090 131073439 131073465 131073468 131074974 131074981 131074997 131074999 131075000 131075004 131076748 131079272 131079273 131079282 131079290 131079292 131079293 131079295 131079735 131079736 131858779 131876041
REH : H16_B2168(h16_B2168)
BPM : BURPS1710b_A1280(hpnA)
PDIO : PDMSB3_3354.1(dfrA)
MAQU : Maq22A_c25235(wcaG)
MTUN : MTUNDRAET4_2109(dfrA)
GBN : GEOBRER4_08480(hpnA)
APAU : AMPC_20810(hpnA_2) AMPC_36120
PBRU : LZC95_17355 LZC95_49060
PRUE : LVJ94_17710 LVJ94_49380
PALJ : LZC94_12460 LZC94_31570 LZC94_44790
PAUU : E8A73_007830 E8A73_025805 E8A73_027450
TRS : Terro_1973 Terro_2339
NTI : DNFV4_00236 DNFV4_00976
NALL : PP769_01875 PP769_05055
NNEO : PQG83_13060 PQG83_16240
BALA : DSM104299_04725(auaH_2)
PARN : NBH00_05775 NBH00_16585
PARQ : DSM112329_01450(auaH_1)
SBAE : DSM104329_03993(auaH_3) DSM104329_04524(auaH_4)
PRUN : PCC7821_02152(dfrA)
NLK : NIES25_38190 NIES25_39940
SCYT : SAMD_38350 SAMD_46550
THUG : KNN16_02930 KNN16_11905
» show all
Taxonomy
Reference
Authors
Heller W, Forkmann G, Britsch L, Grisebach H
Title
Enzymatic reduction of (+)-dihydroflavonols to flavan-3,4-cis-diols with flower extracts from Matthiola incana and its role in anthocyanin biosynthesis.
Journal
Reference
Authors
Stafford HA, Lester HH.
Title
Flavan-3-ol Biosynthesis : The Conversion of (+)-Dihydromyricetin to Its Flavan-3,4-Diol (Leucodelphinidin) and to (+)-Gallocatechin by Reductases Extracted from Tissue Cultures of Ginkgo biloba and Pseudotsuga menziesii.
Journal
Reference
Authors
Fischer D, Stich K, Britsch L, Grisebach H
Title
Purification and characterization of (+)dihydroflavonol (3-hydroxyflavanone) 4-reductase from flowers of Dahlia variabilis.
Journal
Reference
Authors
Li H, Qiu J, Chen F, Lv X, Fu C, Zhao D, Hua X, Zhao Q
Title
Molecular characterization and expression analysis of dihydroflavonol 4-reductase (DFR) gene in Saussurea medusa.
Journal
Other DBs
ExPASy - ENZYME nomenclature database: 1.1.1.219
LinkDB
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