Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy

(2R,3S,4S)-leucoanthocyanidin:NADP+ 4-oxidoreductase

a (2R,3S,4S)-leucoanthocyanidin + NADP+ = a (2R,3R)-dihydroflavonol + NADPH + H+ [RN:R11500]

R11500 > R03123 R03636 R05038 R07998 R07999
Reaction

(2R,3S,4S)-leucoanthocyanidin [CPD:C21418];
NADP+ [CPD:C00006]

This plant enzyme, involved in the biosynthesis of anthocyanidins, is known to act on (+)-dihydrokaempferol, (+)-taxifolin, and (+)-dihydromyricetin, although some enzymes may act only on a subset of these compounds. Each dihydroflavonol is reduced to the corresponding cis-flavan-3,4-diol. NAD+ can act instead of NADP+, but more slowly.

EC 1.1.1.219 created 1989, modified 2016

1  [PMID:24241054]

Heller W, Forkmann G, Britsch L, Grisebach H

Enzymatic reduction of (+)-dihydroflavonols to flavan-3,4-cis-diols with flower extracts from Matthiola incana and its role in anthocyanin biosynthesis.

Planta 165:284-7 (1985)
DOI:10.1007/BF00395052

2  [PMID:16664326]

Stafford HA, Lester HH.

Flavan-3-ol Biosynthesis : The Conversion of (+)-Dihydromyricetin to Its Flavan-3,4-Diol (Leucodelphinidin) and to (+)-Gallocatechin by Reductases Extracted from Tissue Cultures of Ginkgo biloba and Pseudotsuga menziesii.

Plant Physiol 78:791-794 (1985)
DOI:10.1104/pp.78.4.791

3  [PMID:3293532]

Fischer D, Stich K, Britsch L, Grisebach H

Purification and characterization of (+)dihydroflavonol (3-hydroxyflavanone) 4-reductase from flowers of Dahlia variabilis.

Arch Biochem Biophys 264:40-7 (1988)
DOI:10.1016/0003-9861(88)90567-X

4  [PMID:21701830]

Li H, Qiu J, Chen F, Lv X, Fu C, Zhao D, Hua X, Zhao Q

Molecular characterization and expression analysis of dihydroflavonol 4-reductase (DFR) gene in Saussurea medusa.

Mol Biol Rep 39:2991-9 (2012)
DOI:10.1007/s11033-011-1061-2