KEGG   ENZYME: 1.1.1.442
Entry
EC 1.1.1.442                Enzyme                                 
Name
20beta-hydroxysteroid dehydrogenase;
20beta-HSDH;
desE (gene name)
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
Sysname
20beta-dihydrocortisol:NAD+ 20-oxidoreductase
Reaction(IUBMB)
20beta-dihydrocortisol + NAD+ = cortisol + NADH + H+ [RN:R13605]
Reaction(KEGG)
R13605
Substrate
20beta-dihydrocortisol [CPD:C23110];
NAD+ [CPD:C00003]
Product
cortisol [CPD:C00735];
NADH [CPD:C00004];
H+ [CPD:C00080]
Comment
The enzyme, characterized from several gut bacteria, is specific for NADH and is much more active in the reductive direction. cf. the eukaryotic enzyme EC 1.1.1.53, 3alpha(or 20beta)-hydroxysteroid dehydrogenase.
History
EC 1.1.1.442 created 2025
Orthology
K28758  20beta-hydroxysteroid dehydrogenase
Genes
AUH: AWM75_07950
CCAA: KQH81_10320
BADL: BADO_0956
BADO: BBMN23_1035
BFJ: B19861_10860
BKS: BBKW_1136
BCAT: BBCT_1008
BPSC: BBPC_1010
BSCA: BBSC_1118
BLEM: BL8807_01055
BEU: BE0216_10400
 » show all
Reference
1  [PMID:28314858]
  Authors
Devendran S, Mendez-Garcia C, Ridlon JM.
  Title
Identification and characterization of a 20beta-HSDH from the anaerobic gut bacterium Butyricicoccus desmolans ATCC 43058.
  Journal
J Lipid Res 58:916-925 (2017)
DOI:10.1194/jlr.M074914
  Sequence
[ag:WP_051643274]
Reference
2  [PMID:31209107]
  Authors
Doden HL, Pollet RM, Mythen SM, Wawrzak Z, Devendran S, Cann I, Koropatkin NM, Ridlon JM.
  Title
Structural and biochemical characterization of 20beta-hydroxysteroid dehydrogenase from Bifidobacterium adolescentis strain L2-32.
  Journal
J Biol Chem 294:12040-12053 (2019)
DOI:10.1074/jbc.RA119.009390
  Sequence
[badl:BADO_0956]
Other DBs
ExplorEnz - The Enzyme Database: 1.1.1.442
IUBMB Enzyme Nomenclature: 1.1.1.442
ExPASy - ENZYME nomenclature database: 1.1.1.442
BRENDA, the Enzyme Database: 1.1.1.442
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