KEGG ENZYME: 1.14.12.25

ENZYME: 1.14.12.25

Entry

EC 1.14.12.25               Enzyme

Name

p-cumate 2,3-dioxygenase

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor

Sysname

4-isopropylbenzoate:oxygen 2,3-oxidoreductase

Reaction(IUBMB)

p-cumate + NADH + H+ + O2 = (2R,3S)-2,3-dihydroxy-2,3-dihydro-p-cumate + NAD+ [RN:R05247]

Reaction(KEGG)

R05247

Substrate

p-cumate [CPD:C06578];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]

Product

(2R,3S)-2,3-dihydroxy-2,3-dihydro-p-cumate [CPD:C06579];
NAD+ [CPD:C00003]

Comment

The enzyme, characterized from several Pseudomonas strains, is involved in the degradation of p-cymene and p-cumate. It comprises four components: a ferredoxin, a ferredoxin reductase, and two subunits of a catalytic component. The enzyme can also act on indole, transforming it to the water-insoluble blue dye indigo.

History

EC 1.14.12.25 created 2016

Pathway

ec00622

Xylene degradation

ec01100

Metabolic pathways

ec01120

Microbial metabolism in diverse environments

Orthology

K10619

p-cumate 2,3-dioxygenase subunit alpha

K16303

p-cumate 2,3-dioxygenase subunit beta

Genes

RBD:	ALSL_1087 ALSL_1088

PAE:	PA2083 PA2085

PAEV:

N297_2148 N297_2150

PAEI:

N296_2148 N296_2150

PAU:	PA14_37550 PA14_37570

PAG:	PLES_32361 PLES_32381

PAF:	PAM18_2958 PAM18_2960

PNC:	NCGM2_3063 NCGM2_3065

PAEB:

NCGM1900_4396 NCGM1900_4398

PDK:	PADK2_15225 PADK2_15235

PSG:	G655_14675 G655_14685

PRP:	M062_10830 M062_10840

PAEP:

PA1S_15255 PA1S_15265

PAEM:

U769_14855 U769_14865

PAEL:

T223_16535 T223_16545

PAES:

SCV20265_3298 SCV20265_3300

PAEU:

BN889_02279 BN889_02281

PAEG:

AI22_18505 AI22_18515

PAEC:

M802_2145 M802_2147

PAEO:

M801_2147 M801_2149

SECH:

B18_22315 B18_22325

PPF:	Pput_2897 Pput_2898

PPX:	T1E_4248(cbdC) T1E_4249

PMON:

X969_10845 X969_10850

PMOT:

X970_10500 X970_10505

PSES:

PSCI_1386 PSCI_1388

PASI:

LG197_17365 LG197_17370

SPOI:

IMCC21906_02711 IMCC21906_02712

STAW:

NCG89_14290 NCG89_14295

ZAL:	AZF00_03880 AZF00_03885 AZF00_17855 AZF00_17860

TLO:	J9253_01265 J9253_01270

GBI:	PG2T_01290 PG2T_01295 PG2T_14345 PG2T_14350

BAEN:

L3V59_37730 L3V59_37735

BXE:	Bxe_A3555 Bxe_A3556

BXB:	DR64_1254 DR64_1255

PFIB:

PI93_021400 PI93_021405

XYG:	R9X41_00450

THAU:

C4PIVTH_4365(cmtAb) C4PIVTH_4366(cmtAc)

BRK:	CWS35_25065 CWS35_25070

BARH:

WN72_35375 WN72_35380

BGK:	IC762_14175 IC762_14180

RHZ:	RHPLAN_11150 RHPLAN_11160 RHPLAN_44350 RHPLAN_44360

MECQ:

MSC49_16390 MSC49_16400

MON:	G8E03_15190 G8E03_15195

NRE:	BES08_18565 BES08_18570

SMAZ:

LH19_27420 LH19_27425

SWI:	Swit_1757 Swit_1758

SPHD:

HY78_16795 HY78_16800

RDI:	CMV14_03910 CMV14_03915

SPAN:

AWL63_19960 AWL63_19965

SNAP:

PQ455_10990 PQ455_10995

SPHJ:

BSL82_10970 BSL82_10975

BACI:

B1NLA3E_13990 B1NLA3E_13995

OCN:	CUC15_03700 CUC15_03705

VIG:	BKP57_09420 BKP57_09425

VNT:	OLD84_14570 OLD84_14575

VIK:	KFZ58_14470 KFZ58_14475

LAO:	AOX59_08275 AOX59_08280

RUE:	DT065_15140 DT065_15145

SALE:

EPH95_15060 EPH95_15065

NDT:	L1999_17815 L1999_17820

PBJ:	VN24_05635 VN24_05640

PNP:	IJ22_32370 IJ22_32380

ADAU:

NZD86_05725 NZD86_05730

EFF:	skT53_01990

PSHE:

QWY16_07620 QWY16_07625

SPAE:

E2C16_04425 E2C16_04430

EUZ:	DVS28_a1943 DVS28_a1944 DVS28_a1989

MMAN:

MMAN_42610 MMAN_42620

MSAK:

MSAS_43620 MSAS_43630

MPAG:

C0J29_30955 C0J29_30960

MCOO:

MCOO_07710 MCOO_07720

MNE:	D174_16895 D174_16905 D174_16915 D174_16920

MYN:	MyAD_16560 MyAD_16570 MyAD_16580 MyAD_16585

MHAS:

MHAS_04025(bnzB) MHAS_04026(cbdA_2)

MANY:

MANY_01930 MANY_01940

MAUS:

JN090_26185 JN090_26190

ROP:	ROP_46330(cmtA2) ROP_46340(cmtA1)

RRZ:	CS378_10910 CS378_10915

RHOD:

AOT96_33475 AOT96_33510

RRT:	4535765_00187(cmtA1_1) 4535765_00188(cmtA2_1) 4535765_00374(cmtA2_2) 4535765_00375(cmtA1_2)

RKO:	JWS14_14210 JWS14_14215

ROZ:	CBI38_27935 CBI38_27940 CBI38_28895 CBI38_28900

RPSK:

JWS13_02050 JWS13_02055

RHOU:

KZJ41_22825 KZJ41_22830

RHOJ:

JVH1_23295 JVH1_23300

GPD:	GII33_08510 GII33_08525

SALS:

SLNWT_6906 SLNWT_6907

SCZ:	ABE83_01985 ABE83_01990

STSI:

A4E84_36025 A4E84_36030

SALW:

CP975_27200 CP975_27205 CP975_33970 CP975_33975

SBAT:

G4Z16_01225

SHUN:

DWB77_04977(antA) DWB77_04978(hcaF)

SAKB:

K1J60_04500 K1J60_04505

KBU:	Q4V64_14275 Q4V64_14280

PSIM:

KR76_24910 KR76_24915

FSY:	FsymDg_2033 FsymDg_2034

AMQ:	AMETH_1199(benA) AMETH_1200

AACD:

LWP59_08660 LWP59_08665 LWP59_25375 LWP59_25380

PDX:	Psed_4287 Psed_4289

PSEQ:

AD006_28390 AD006_28395 AD006_28760 AD006_28765

PECQ:

AD017_28780 AD017_28785 AD017_29150 AD017_29155

PBRO:

HOP40_30820 HOP40_30825

PPEL:

H6H00_18645 H6H00_18650

SSYI:

EKG83_16160 EKG83_16165

KUT:	JJ691_102160 JJ691_102180

PMAD:

BAY61_11485 BAY61_11490 BAY61_20660 BAY61_20690

UME:	RM788_02665 RM788_02670

MICH:

FJK98_14410 FJK98_14415

MCAB:

HXZ27_11850 HXZ27_11855 HXZ27_13045 HXZ27_13050

VMA:	VAB18032_10630 VAB18032_10635

MFEU:

H1D33_26160 H1D33_26165

PRY:	Prubr_43520(hcaF) Prubr_43530

DROS:

Drose_15490 Drose_15495

JCY:	M6B22_10270 M6B22_10275

PARN:

NBH00_20065 NBH00_20075

SBAE:

DSM104329_02439(cmtAc) DSM104329_02440(cmtAb)

TTL:	TtJL18_2435 TtJL18_2436

» show all

Reference

1 [PMID:845117]

Authors

DeFrank JJ, Ribbons DW

Title

p-cymene pathway in Pseudomonas putida: initial reactions.

Journal

J Bacteriol 129:1356-64 (1977)
DOI:10.1128/JB.129.3.1356-1364.1977

Reference

2 [PMID:7204334]

Authors

Wigmore GJ, Ribbons DW

Title

p-Cymene pathway in Pseudomonas putida: selective enrichment of defective mutants by using halogenated substrate analogs.

Journal

J Bacteriol 143:816-24 (1980)
DOI:10.1128/JB.143.2.816-824.1980

Reference

3 [PMID:7592495]

Authors

Eaton RW, Chapman PJ

Title

Formation of indigo and related compounds from indolecarboxylic acids by aromatic acid-degrading bacteria: chromogenic reactions for cloning genes encoding dioxygenases that act on aromatic acids.

Journal

J Bacteriol 177:6983-8 (1995)
DOI:10.1128/JB.177.23.6983-6988.1995

Reference

4 [PMID:8631713]

Authors

Eaton RW.

Title

p-Cumate catabolic pathway in Pseudomonas putida Fl: cloning and characterization of DNA carrying the cmt operon.

Journal

J Bacteriol 178:1351-62 (1996)
DOI:10.1128/JB.178.5.1351-1362.1996

Sequence

[ppf:Pput_2898 Pput_2897 Pput_2894 Pput_2899]