Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
dihydromonacolin L acid,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (3-hydroxylating)
Reaction(IUBMB)
dihydromonacolin L acid + O2 + [reduced NADPH---hemoprotein reductase] = monacolin L acid + [oxidized NADPH---hemoprotein reductase] + 2 H2O (overall reaction) [RN:R10793];
(1a) dihydromonacolin L acid + O2 + [reduced NADPH---hemoprotein reductase] = 3alpha-hydroxy-3,5-dihydromonacolin L acid + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R10791];
(1b) 3alpha-hydroxy-3,5-dihydromonacolin L acid = monacolin L acid + H2O (spontaneous) [RN:R10792]
dihydromonacolin L acid [CPD:C20851];
O2 [CPD:C00007];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
3alpha-hydroxy-3,5-dihydromonacolin L acid [CPD:C20852]
Product
monacolin L acid [CPD:C20853];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001];
3alpha-hydroxy-3,5-dihydromonacolin L acid [CPD:C20852]
Comment
A cytochrome P-450 (heme-thiolate) protein. The dehydration of 3alpha-hydroxy-3,5-dihydromonacolin L acid is believed to be spontaneous [1,2]. The enzyme from fungi also catalyses the reaction of EC 1.14.14.125, monacolin L hydroxylase [3].
History
EC 1.14.14.124 created 2014 as EC 1.14.13.197, transferred 2018 to EC 1.14.14.124
