KEGG   ENZYME: 1.14.14.144
Entry
EC 1.14.14.144              Enzyme                                 
Name
abieta-7,13-diene hydroxylase;
abietadiene hydroxylase (ambiguous)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
abieta-7,13-diene,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (18-hydroxylating)
Reaction(IUBMB)
abieta-7,13-diene + [reduced NADPH---hemoprotein reductase] + O2 = abieta-7,13-dien-18-ol + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R06351]
Reaction(KEGG)
R06351
Substrate
abieta-7,13-diene [CPD:C11878];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
abieta-7,13-dien-18-ol [CPD:C11882];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
A cytochrome P-450 (heme-thiolate) protein. This enzyme catalyses a step in the pathway of abietic acid biosynthesis. The activity has been demonstrated in cell-free stem extracts of Abies grandis (grand fir) and Pinus contorta (lodgepole pine). Activity is induced by wounding of the plant tissue [2].
History
EC 1.14.14.144 created 2009 as EC 1.14.13.108, modified 2012, transferred 2018 to EC 1.14.14.144
Pathway
ec00904  Diterpenoid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Reference
1  [PMID:8311462]
  Authors
Funk C, Croteau R
  Title
Diterpenoid resin acid biosynthesis in conifers: characterization of two cytochrome P450-dependent monooxygenases and an aldehyde dehydrogenase involved in abietic acid biosynthesis.
  Journal
Arch Biochem Biophys 308:258-66 (1994)
DOI:10.1006/abbi.1994.1036
Reference
2  [PMID:12232380]
  Authors
Funk C, Lewinsohn E, Vogel BS, Steele CL, Croteau R
  Title
Regulation of Oleoresinosis in Grand Fir (Abies grandis) (Coordinate Induction of Monoterpene and Diterpene Cyclases and Two Cytochrome P450-Dependent Diterpenoid Hydroxylases by Stem Wounding).
  Journal
Plant Physiol 106:999-1005 (1994)
DOI:10.1104/pp.106.3.999
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.144
IUBMB Enzyme Nomenclature: 1.14.14.144
ExPASy - ENZYME nomenclature database: 1.14.14.144
BRENDA, the Enzyme Database: 1.14.14.144
LinkDB

KEGG   REACTION: R06351
Entry
R06351                      Reaction                               
Name
abieta-7,13-diene,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (18-hydroxylating)
Definition
(-)-Abietadiene + [Reduced NADPH---hemoprotein reductase] + Oxygen <=> Abietinol + [Oxidized NADPH---hemoprotein reductase] + H2O
Equation
C11878 + C03024 + C00007 <=> C11882 + C03161 + C00001
Reaction class
RC00257  C11878_C11882
Enzyme
1.14.14.144
Pathway
rn00904  Diterpenoid biosynthesis
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Brite
Enzymatic reactions [BR:br08201]
 1. Oxidoreductase reactions
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.14  With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.14.144
     R06351  (-)-Abietadiene + [Reduced NADPH---hemoprotein reductase] + Oxygen <=> Abietinol + [Oxidized NADPH---hemoprotein reductase] + H2O
Other DBs
RHEA: 26220
LinkDB

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