vitamin D 25-hydroxylase;
vitamin D2 25-hydroxylase;
vitamin D3 25-hydroxylase;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
A microsomal enzyme isolated from human and mouse liver that bioactivates vitamin D3. While multiple isoforms (CYP27A1, CYP2J2/3, CYP3A4, CYP2D25 and CYP2C11) are able to catalyse the reaction in vitro, only CYP2R1 is thought to catalyse the reaction in humans in vivo . The direct electron donor to the enzyme is EC 184.108.40.206, NADPH---hemoprotein reductase.
EC 220.127.116.11 created 2012 as EC 18.104.22.168, transferred 2016 to EC 22.214.171.124