KEGG ENZYME: 1.4.1.28

ENZYME: 1.4.1.28

Entry

EC 1.4.1.28                 Enzyme

Name

secondary-alkyl amine dehydrogenase [NAD(P)+];
AmDH (ambiguous);
amine dehydrogenase (ambiguous)

Class

Oxidoreductases;
Acting on the CH-NH2 group of donors;
With NAD+ or NADP+ as acceptor

Sysname

secondary-alkyl amine:NAD(P)+ oxidoreductase (deaminating)

Reaction(IUBMB)

a secondary-alkyl amine + H2O + NAD(P)+ = a ketone + NH3 + NAD(P)H + H+ [RN:R13021 R13022]

Reaction(KEGG)

R13021 R13022

Substrate

secondary-alkyl amine [CPD:C22522];
H2O [CPD:C00001];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006]

Product

ketone [CPD:C01450];
NH3 [CPD:C00014];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080]

Comment

The enzyme has been shown to react preferentially with short-chain ketones such as cyclohexanone, primary amine groups attached to secondary alkyl groups, or D- and L-amino acids. It also reduces aldehydes to primary amines. Cosubstrate preference depends on the substrate.

History

EC 1.4.1.28 created 2022

Orthology

K25950

secondary-alkyl amine dehydrogenase [NAD(P)+]

Genes

PRG:	RB151_036990

PALA:

CO695_07830

PHEI:

NCTC12003_03184

PRQ:	CYG50_20115

PRJ:	NCTC6933_03276

PMAG:

JI723_16295

PHAG:

PZ638_16715

PZJ:	QS795_014265

VIT:	ADP71_22650

VST:	LVJ81_02535

LHK:	LHK_02682(dapB-1)

DDS:

Ddes_0477

DFI:	AXF13_02450

DEF:	CNY67_12015

CFUS:

CYFUS_009021

TPAV:

HRQ91_01365

SSM:	Spirs_3711

EMI:

Emin_0479

EPO:

Epro_1033

CSOA:

LIS82_07275

BDA:	FSZ17_12900

PKB:	B4V02_12565

PTHI:

NDS46_20650

PDY:	QJQ58_01415

PSPN:

L1F29_30880

AUD:	PO771_02520

SMU:

SMU_527

SMC:	SmuNN2025_1443

SMUT:

SMUGS5_02315

SMJ:	SMULJ23_1460(dapB1)

SMUA:

SMUFR_0455

SSA:

SSA_2021

SGO:

SGO_0387

SSR:	SALIVB_1352

SPAT:

A0O21_00670

SRAT:

FY406_05215

STRG:

SRT_15900

SFER:

NCTC12278_01801

SCHJ:

DDV21_002215

SOS:	INT76_10525

SZG:	J5M87_01220

LGN:	ABM34_07565

LDX:	LH506_05355

EMU:

EMQU_2449

EAV:	EH197_22580

VPI:	BW732_10195

VFV:	K5K99_01910

GSG:	CYJ72_005115

GAD:	K8O88_08545

FSA:	C5Q98_04800

RGV:	NQ502_12070

SMAN:

C12CBH8_15580

ACOL:

K5I23_10270

RUK:	A4V00_00735

AMUR:

ADH66_08365

ELM:

ELI_1411

EMT:	CPZ25_000470

ELIM:

B2M23_10835

AWO:	Awo_c34400(dapB2)

LACY:

A4V08_05170

CSH:	Closa_0973

RGN:	RGna_09095

RLR:	NQ541_01535

CBOL:

CGC65_17860

CASP:

NQ535_13680

ECLF:

QMP25_13840

HHJ:	NQ487_07515

LBW:	C3V36_05155 C3V36_09895

FAA:	HMPREF0389_01077

CMIU:

B1H56_09445

TEP:	TepRe1_0280

TAE:	TepiRe1_0312

HOV:	EQF90_007930

SPOA:

EQM13_14710

ERH:

ERH_1328

ERS:	K210_04990

ERL:	AOC36_02945

ERI:	EEI45_00350

EEH:	LLS04_01425

EBM:	SG0102_18650

FIT:	Fi14EGH31_22290

ERM:	EYR00_10680

CMIT:

NQ499_01290

OLS:

Olsu_0652

TIO:	INP52_04835

MSM:	MSMEG_3317(dapB)

MSG:	MSMEI_3231

MSB:	LJ00_16480

MSN:	LI99_16485

MSH:	LI98_16490

MVQ:	MYVA_1576(dapB)

RHOO:

RA302_34450

SANL:

KZO11_34405

SRC:	M271_34125

LDN:	H9L06_04775

BROO:

brsh051_07550

NOCL:

KM427_02365

SACG:

FDZ84_28340

BLO:

BL0836

BLJ:

BLD_0569

BLF:

BLIF_0905

BLL:

BLJ_0914

BLB:	BBMN68_575

BLM:

BLLJ_0783

BLK:	BLNIAS_01599

BLG:

BIL_10530

BLZ:	BLGT_04910

BLX:	GS08_04730

BLA:	BLA_0924(dapB)

BLC:	Balac_1124

BLT:	Balat_1124

BBB:

BIF_02266

BBC:

BLC1_1080

BNM:	BALAC2494_00125

BLV:

BalV_1084

BLW:

W7Y_1124

BLS:

W91_1149

BANI:

BL12_05605

BANL:

BLAC_05660

BNI:	BANAN_05535

BANM:

EN10_05695

BCHO:

BcFMB_08615

ABAO:

BEQ56_08085

PPIO:

CE91St28_24130

ARAM:

KAR29_01470

CSOM:

MKD34_04580

HARA:

AArcS_1758

NGE:	Natgr_2708

» show all

Reference

Authors

Itoh N, Yachi C, Kudome T.

Title

Determining a novel NAD+-dependent amine dehydrogenase with a broad substrate range from Streptomyces virginiae IFO 12827: purification and characterization.

Journal

Journal of Molecular Catalysis B: Enzymatic 10:281-290 (2000)

Reference

Authors

Mayol O, David S, Darii E, Debard A, Mariage A, Pellouin V, Petit JL, Salanoubat M, de Berardinis V, Zaparucha A, Vergne-Vaxelaire C.

Title

Asymmetric reductive amination by a wild-type amine dehydrogenase from the thermophilic bacteria Petrotoga mobilis.

Journal

Catalysis Science & Technology 6:7421-7428 (2016)

Reference

Authors

Mayol O, Bastard K, Beloti L, Frese A, Turkenburg JP, Petit JL, Mariage A, Debard A, Pellouin V, Perret A, de Berardinis V, Zaparucha A, Grogan G, Vergne-Vaxelaire C.

Title

A family of native amine dehydrogenases for the asymmetric reductive amination of ketones.

Journal

Nature Catalysis 2:324-333 (2019)

Reference

Authors

Lee S, Jeon H, Giri P, Lee UJ, Jung H, Lim S, Sarak S, Khobragade TP, Kim BG, Yun H.

Title

The reductive amination of carbonyl compounds using native amine dehydrogenase from Laribacter hongkongensis.

Journal

Biotechnol Bioprocess Eng 26:384-391 (2021)

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