KEGG   ENZYME: 1.4.99.6
Entry
EC 1.4.99.6                 Enzyme                                 
Name
D-arginine dehydrogenase;
D-amino-acid:(acceptor) oxidoreductase (deaminating);
D-amino-acid dehydrogenase;
D-amino-acid:acceptor oxidoreductase (deaminating)
Class
Oxidoreductases;
Acting on the CH-NH2 group of donors;
With unknown physiological acceptors
Sysname
D-arginine:acceptor oxidoreductase (deaminating)
Reaction(IUBMB)
D-arginine + acceptor + H2O = 5-guanidino-2-oxopentanoate + NH3 + reduced acceptor (overall reaction) [RN:R11018];
(1a) D-arginine + acceptor = iminoarginine + reduced acceptor [RN:R11016];
(1b) iminoarginine + H2O = 5-guanidino-2-oxopentanoate + NH3 (spontaneous) [RN:R11017]
Reaction(KEGG)
R11016 R11017 R11018;
(other) R01342 R07166
Substrate
D-arginine [CPD:C00792];
acceptor [CPD:C00028];
H2O [CPD:C00001];
iminoarginine [CPD:C21026]
Product
5-guanidino-2-oxopentanoate [CPD:C03771];
NH3 [CPD:C00014];
reduced acceptor [CPD:C00030];
iminoarginine [CPD:C21026]
Comment
Contains a non-covalent FAD cofactor. The enzyme, which has been isolated from the bacterium Pseudomonas aeruginosa PAO1, forms with EC 1.4.1.25, L-arginine dehydrogenase, a two-enzyme complex involved in the racemization of D- and L-arginine. The enzyme has a broad substrate range and can act on most D-amino acids with the exception of D-glutamate and D-aspartate. However, activity is maximal with D-arginine and D-lysine. Not active on glycine.
History
EC 1.4.99.6 created 1972 as EC 1.4.99.1, transferred 2015 to EC 1.4.99.6, modified 2017
Pathway
ec00470  D-Amino acid metabolism
ec01100  Metabolic pathways
Orthology
K19746  D-arginine dehydrogenase
Genes
RGLCS053_15415
DJICH75_22885
DJAHY57_05670
DTLH8F01_12465
LRZBJI69_19345
LPYFIV34_17165
PAEPA3863(dauA)
PAEVN297_3989
PAEIN296_3989
PAUPA14_14010
PAPPSPA7_1242 PSPA7_2008
PAGPLES_11111
PAFPAM18_1075
PNCNCGM2_5053
PAEBNCGM1900_1110
PDKPADK2_05000
PSGG655_05380
PRPM062_20425
PAEPPA1S_05505
PAERPA1R_gp1757
PAEMU769_05540
PAELT223_05455
PAESSCV20265_1134
PAEUBN889_04294
PAEGAI22_28110
PAECM802_3987
PAEOM801_3855
PMYPmen_3567
PMKMDS_3832
PREPCA10_11010
PPSEBN5_1000(Ppsl_3554)
PFUWKF707C_10280
PALCA0T30_06950
PCQPcP3B5_17690(soxA_1) PcP3B5_50450(soxA_3)
PMUIG4G71_09095 G4G71_20780
PPUPP_2246(dauA)
PPFPput_3492
PPGPputGB1_1877
PPTPPS_1856
PPBPPUBIRD1_3408
PPIYSA_01272
PPXT1E_5405
PPUHB479_09190
PPUNPP4_35420
PPUDDW66_2104
PMONX969_07145
PMOTX970_07120
PPJRK21_03179
PFLPFL_0805
PPRCPFLCHA0_c08160 PFLCHA0_c10630
PPROPPC_0825 PPC_1081
PFEPSF113_4743 PSF113_4854
PFNHZ99_11880
PFOPfl01_0969 Pfl01_2315
PFCPflA506_2227
PFBVO64_5501
PMANOU5_2436
PTVAA957_03150
PCGAXG94_09925
PVRPverR02_12345
PAZOAYR47_18375
PRXHRH33_11180
PFWPF1751_v1c20440
PFFPFLUOLIPICF726495
PFXA7318_09930
PENPSEEN3860
PBAPSEBR_a4581 PSEBR_a4688
PPUUPputUW4_04462
PDRH681_16740 H681_19280
PSKU771_12275
PKCPKB_0988
PCHEY04_04565
PCZPCL1606_50600
PCPJM49_25080
PALKPSAKL28_08830 PSAKL28_17300
PSEMTO66_05255
PSOSPOS17_0787 POS17_1041
PKRAYO71_07585
PFKPFAS1_16740
PANRA7J50_2220
PSETTHL1_1154
PSILPMA3_04245
PYMAK972_0849
PKEDLD99_05205
PALLUYA_18420
PUMHGP31_04840
POJPtoMrB4_46070(dauA)
PGYAWU82_19035 AWU82_29055
PLALFXN65_05130
PSEJHNQ25_07070
PVKEPZ47_24280
PBZGN234_23560 GN234_24065
PKHJLK41_19580
PTYJWV26_04705
PATAJWU58_22705
PDWBV82_3868 BV82_4678
PZEHU754_006320
PMOEHV782_005895
PCAMHNE05_02890
PSEPC4K39_0794 C4K39_1053
PTRTHU722_0011755
PSAMHU731_027920
PTZHU718_005955
PQIKH389_23315
PSHHHU773_013545 HU773_022790
PXNHU772_008635
PHVHU739_012665
PANHHU763_009280
PMUYKSS95_18575
PAZEKSS91_23800
PALVKSS97_06340
PORYEJA05_09805
PTKEXN22_22880
PTWTUM18999_49980(dauA)
PZAHU749_024090 HU749_024675
PIEHU724_005720
PTAENCTC10697_01860
PGFJ0G10_06120
AFUSEYZ66_08380
SOKD0B54_15685
REHH16_B1130(h16_B1130)
CNCCNE_2c10920
RMERmet_5086
CBWRR42_m2259
CCUPBKK81_28350
CUPBKK80_28025
CUUBKK79_33940
PCAFDSC91_007188
BPTBpet0809
BTRMSAMEA390648700389 SAMEA390648702048
AXYAXYL_01314
AXONH44784_047341
AXNAX27061_2904
AXXERS451415_02882
ADTAPT56_14020
AISBUW96_19695
ASWCVS48_21350
ACHRC2U31_06255
ACHBDVB37_15555
ADYHLG70_16080
AMIMMIM_c13060
CDNBN940_09291
AFAUZ73_18305
AFQAFA_08995
AAQUD3M96_10230
AAMMFE795_08385
OUEI6G29_12765
PACRFXN63_19550
PARKLSG25_19070
RFRRfer_4053
RACRA876_14190
RHYRD110_23300
POLBpro_1468
AAVAave_2431
AJSAjs_2247
AAAAcav_2785
ACKC380_11235
ACIOEAG14_10890
AANTHUK68_00285
ARADKI609_12710
DACDaci_0166 Daci_0820
DELDelCs14_0168 DelCs14_5711
DTSBI380_20590 BI380_22575
DHKBO996_02165 BO996_04170
DLAI6G47_20530 I6G47_22535
VAPVapar_1558
VPEVarpa_1705
VPDVAPA_1c16530
VBOCKY39_09685
VAMC4F17_10650
CTTCtCNB1_4765
CTESO987_28325
CKEB5M06_05675
CSERCCO03_17635
COFFOZ74_07020
CAQTKAQ61_15905
CODOLAD35_19395
RTEAHK414_19655
OTKC6570_14545
OTDJ1M35_06175 J1M35_06215
LIML103DPR2_01068(lhgO_1)
HYLLPB072_12625
HYCE5678_08975
HPSEHPF_07855(lhgO1)
HTNKI616_18325
DPYBA022_05965
DIHG7047_24220
DRGH9K76_01185
DIADtpsy_1627
SIMPC6571_07625
MELAC6568_17250
MJELVC68_02135
KIAG8A07_11385
HSEHsero_4377(dadA)
HSZACP92_21860
HHTF506_04640
HRBHrubri_1178
HEEhmeg3_03710
HHFE2K99_06755
HFRG5S34_07025
MASYDPH57_04865
MALIEYF70_08225
MUMFCL38_10535
MFLAGO485_21195
NOKFAY22_17550
RGERGE_37740
RBNRBXJA2T_15168
MIUABE85_14445
URUDSM104443_01419(dauA_2)
ACOMCEW83_05870
APPCAP2UW1_0897
HOHHoch_0434
MLOmll6667 mlr1836
MLNA9174_17330 A9174_30230
MOPMesop_3325
MAMMesau_03212
MAMOA6B35_10455
MESMEJ066_08025
MESPC1M53_11125
MHUAMCHK_0585 MCHK_3415
MJREB229_17570 EB229_30830
MERDEB233_14855 EB233_27035
MESRFGU64_15660
MESOBSQ44_03485 BSQ44_08185
HOEIMCC20628_02682
AAKAA2016_2165
AMIHCO731_02274(soxA_2)
PHTBLM14_28370
NIYFQ775_03260 FQ775_08335
NKIKW403_11415
KMNHW532_14705
SMESM_b21179
SMKSinme_4859
SMQSinmeB_3818
SMXSM11_pD0750
SMIBN406_05813
SMEGC770_GR4pD0732
SMELSM2011_b21179
SMERDU99_29175
SMDSmed_4669
SIXBSY16_1570
EADOV14_a0498
EAHFA04_22630
EMXFKV68_28205
SHZshn_06905
SZOK8M09_06140
SOJK6301_05300
BVVBHK69_09040
BOIBLM15_18220
RHJHZY79_02070
NTDEGO55_13025
MMYRMXMO3_01389
NOHG5V57_06945 G5V57_31250
RBMTEF_10460
PSFPSE_p0308
LAPACP90_00375 ACP90_22400
LABPFJ695_25050
LABTFIU93_00145(thiO) FIU93_05460(hcnC1)
LAGGB0E33_05825 B0E33_11885
SIWGH266_04500
CAKCaul_3007
CMBCSW64_13585
CAUFCSW63_03880
CAULKCG34_25335
PZUPHZ_c0678
BMEDGYM46_14220
SILSPO0642 SPO2001
SITTM1040_0490
RUAD1823_00965
RUTFIU92_12145
JANJann_0926
RLIRLO149_c020760
RPONG3256_14560
PGAPGA1_c28200
PGLPGA2_c26200
PGDGal_00605
PHPPhaeoP97_00683
PPICPhaeoP14_02582
PHQD1820_08810
OATOAN307_c42860
OAROA238_160p1280 OA238_c04930
OTMOSB_05370
OCTFTO60_13415
LMDMETH_03975
LEJETW24_05290
LAQUR2C4_13110
REDroselon_00380
MALGMALG_01246
TPROGa0080559_TMP2990
SUAMBOO69_14015 BOO69_20650
SULZC1J03_18515
SULIC1J05_07125 C1J05_17045
SULDB5M07_16440
SPOTG6548_05795
SMEDJNX03_12555 JNX03_17710 JNX03_19520
SINLDSM14862_02067(dauA_2) DSM14862_03230(dauA_3)
SPSESULPSESMR1_00307(dauA)
TOMBWR18_12810
RMMROSMUCSMR3_01932(dauA)
ROKRAK1035_1254
ROMEI983_02620
AHTANTHELSMS3_00302
THAACFI11_17865
OCDFHY55_12450
MARUFIU81_03435
MALUKU6B_10290
TGLHFZ77_08575
HATRC74_12900
RSPRSP_3563
RSHRsph17029_3246
RSQRsph17025_3225
RSKRSKD131_3731
CAZTLV780_15380
RCPRCAP_rcc00529
RHPLPB142_03940
RBLB6K69_07390
PAMIJCM7686_0903
PYEA6J80_12620
PCONB0A89_09480
PARUCYR75_03890
PMUTDPM13_11080
PARSDRW48_03780
PTPRCA23_c21220
RSUNHU_00784
RHMB5V46_04770
RHCRGUI_0876
RMBK529_002215
DAAAKL17_0635
DONBSK21_11180
LVSLOKVESSMR4R_02746(dauA)
GEHHYN69_00775
GFUKM031_13145
TAWEI545_13065
SEDIEBB79_17360
HMLHmaOT1_10390
BOOE2K80_14250
PSEBEOK75_17105
PAEDG5B38_17270
MONG8E03_14395
PAMOBAR1_13010
PSHQF3W81_14375 F3W81_20400
POZI0K15_05475
PALWPSAL_025610(dauA)
PPAFI8N54_15180
CACTHZ995_10230
RBZB9057_02435
THASC6Y53_03785 C6Y53_05020 C6Y53_10890
HDHG5B40_13310
GCEKYE46_15810
NSMJO391_02705
CBOTATE48_12685
ZMOZMO1441
ZMNZa10_1748
ZMMZmob_1668
ZMBZZ6_1641
ZMIZCP4_1691
ZMCA265_01689
ZMRA254_01689
ZMPZymop_1628
NARSaro_1083
NPPPP1Y_AT19221
NPNJI59_16885
NREBES08_08245
NOVTQ38_006535 TQ38_020100
NOTC7W88_15945
NORFA702_07030
NGFFRF71_01620
NOGGKE62_13015
NDRHT578_06195
SALSala_1132
SPHKSKP52_09465
SPHPLH20_10065
SMAGAN936_07275 AN936_08975
SMAZLH19_07415 LH19_09035
STERAOA14_13970
SGISGRAN_1908 SGRAN_2687 SGRAN_3674(dauA)
SPHQBWQ93_07970
SPHOC3E99_05515 C3E99_09515 C3E99_13740
SPHXE5675_11495
SPHUSPPYR_1791
SWISwit_1429 Swit_4871
SPHDHY78_00070 HY78_19420
RDICMV14_10975
SPHMG432_07455
STAXMC45_05645
SPHITS85_02500
SSANNX02_04735
SNJA7E77_05470
SMYBJP26_01030
SPANAWL63_14290
SKRBRX40_20240 BRX40_21710
SPLMBXU08_08995 BXU08_16650
SPLKAV944_15060
SPKCKC8_11005
SPHCCVN68_09715 CVN68_20120
SPHFDM480_03375
SPHAD3Y57_16510
SPAUDRN02_008610
SECHB18_02360
SLUTH9L13_06325 H9L13_09270
SLANGV829_00255 GV829_13505
SDHH9L15_00425
SSEDH9L14_08410
SPAPH3Z74_14505
SXAFMM02_09530
SPAIFPZ24_02695
SPIIG7077_01070
SSING7078_08330
SSAUH8M03_01205 H8M03_01840
SARIH5J25_05495
SCHYGVO57_05645 GVO57_07670
SSUAFPZ54_09880
SJPSJA_C1-24430
SINBSIDU_02795
SCHSphch_1363
SSYSLG_03600
SYBTZ53_14805
SBDATN00_09310
SPMIK663_12205
SPHBEP837_02152
SPHRBSY17_2402
SPHTK426_14930
SHYDCJD35_11070
SYAA6768_15165
SCLOSCLO_1029110
SPYGYGS_C1P0306
SUFLFIL70_01330
SBARH5V43_01870
SAMISAMIE_1003480
SPPHKFK14_07635 KFK14_15675
CIJWG74_07375
SPHGAZE99_10830
SFLASPHFLASMR4Y_00057(dauA)
SPHYCHN51_11820
SPHLLPB140_09560
BLASBSY18_2122
BFWB5J99_02180
SPZRG5C33_04670 G5C33_05940
PALGHFP57_05345 HFP57_07185
PARJJ4G78_15205
PHAOHF685_06970
SMICSmB9_01240(dauA_1) SmB9_04240 SmB9_15660 SmB9_16330(dauA_2)
SPHSETR14_01540 ETR14_25785
SANDH3309_07240
POQKZX46_05855
AEPAMC99_01921
ALBAEB_P3297
ALHG6N82_02340 G6N82_12425
AAYWYH_01402(hcnC)
AMXAM2010_388
ANHA6F65_02312(hcnC)
ADOA6F68_02824(hcnC)
TFVIDJ81_14750
CNAAB433_13770
CMANA9D14_02645
ERYCP97_12340
EGNBMF35_a0235 BMF35_a0579
EFVCHH26_06105 CHH26_12475
ELIELI_01540
ELQGa0102493_11244
ERKCD351_05840 CD351_08730
ERRDVR09_06090 DVR09_12050
ERFFIU90_05660(hcnC)
EMVHQR01_01655 HQR01_11700
PNSA9D12_08635 A9D12_10900
PORLBG023_111238 BG023_111694
PHZCHX26_06780 CHX26_09900
POTE2E27_13225 E2E27_15815
ROSCTJ15_01470
RMUCFOB66_02390
SRFLHU95_02460
RAPRHOA_5881(dauA)
SHUMSTHU_04150(dauA_1) STHU_07190(dauA_2) STHU_47910
SVCSTVA_05710(dauA_1) STVA_47980(dauA_2)
STELSTAQ_40020(dauA_1) STAQ_40870(dauA_2)
AZLAZL_c00540
ALIAZOLI_p30350
ABQABAZ39_22175
ABFAMK58_19400
ATIAL072_15465
AZTTSH58p_27935
AZMDM194_17675
AZZDEW08_22860
AOZHUE56_03910
NAOY958_15890 Y958_26370
NCBC0V82_18765
SKTIGS68_04410
SMUCJL100_004590
SROEJL101_004320
RCERC1_3715
TMOTMO_1029
FERFNB15_12085
HTQFRZ44_34250(dauA) FRZ44_42190(dauA)
HADHFRZ61_33460(dauA) FRZ61_40360(dauA)
TXITH3_10910
THACCSC3H3_10645
TIIDY252_13820
MAGQMGMAQ_2448
ELIOKO353_00300
ECOGFIV45_00605 FIV45_16535
SNEANBZ79_18245
LYBC3943_19145
MMAGMMAD_03290(dauA)
MABMAB_4739
MMVMYCMA_03130
MABBMASS_4780
MABLMMASJCM_4798
MCHEBB28_24110
MIZBAB75_26720
MSTEMSTE_04881
MSAOMYCSP_22090
MSALDSM43276_04562(hcnC)
ASDAS9A_1478
CCYCSCMU_14870
RHBNY08_1989
RFAA3L23_00322(hcnC_1)
RHSA3Q41_03085(hcnC_2)
GOQACH46_00920
GOCCXX93_02395
GAMGII34_22110
GOILK459_11300
SRTSrot_0241
STRCAA958_24710
SPAVSpa2297_28260
SPHVF9278_41820
ARTArth_1312
ARRARUE_c13780
ARMART_4159
AREAL755_06705
ARHAHiyo8_15420
ARZAUT26_06375
ARUASPU41_11850
ARQBWQ92_03330
ARNCGK93_07585
ARXARZXY2_2577
ARTHC3B78_06305
ARTPE5206_16830
AAGINCTC2676_1_00870
AJGKKR91_05280
ASUNKG104_05590
AAUAAur_1463
PUEFV140_07185
PNVJMY29_06855
ACHAchl_1355
APNAsphe3_13200
PSULAU252_03685
PSNINIBR502771_19010
PSEYGU243_23090
KRHKRH_16700
KFVAS188_08120
KIIKocCE7_09010
KRSEQG70_12135
KODHBK84_08885
KVRCIB50_0001423(dauA)
SATKSA2016_1571
AULDCC27_005830
ICAIntca_0824
NCANoca_1837
PSIMKR76_17635
PDXPsed_2739
PSEEFRP1_07110
PSEHXF36_07930
PSEQAD006_14725
PECQAD017_22550
PAUTPdca_43650
PBROHOP40_18780
ALOCRK55609
MSAGGCM10017556_12120(dauA)
CATICS0771_32840(dauA)
DVCDvina_47570
EKEEK0264_07925
IAMHC251_23840
MINMinf_1810(dadA)
MKCkam1_525
MEAPMTHMO_0829(dauA)
TPXTurpa_3640
 » show all
Reference
1  [PMID:5925166]
  Authors
Tsukada K.
  Title
D-amino acid dehydrogenases of Pseudomonas fluorescens.
  Journal
J Biol Chem 241:4522-8 (1966)
Reference
2  [PMID:19139398]
  Authors
Li C, Lu CD
  Title
Arginine racemization by coupled catabolic and anabolic dehydrogenases.
  Journal
Proc Natl Acad Sci U S A 106:906-11 (2009)
DOI:10.1073/pnas.0808269106
  Sequence
[pae:PA3863]
Reference
3  [PMID:20809650]
  Authors
Fu G, Yuan H, Li C, Lu CD, Gadda G, Weber IT
  Title
Conformational changes and substrate recognition in Pseudomonas aeruginosa D-arginine dehydrogenase.
  Journal
Biochemistry 49:8535-45 (2010)
DOI:10.1021/bi1005865
  Sequence
[pae:PA3863]
Reference
4  [PMID:20932054]
  Authors
Yuan H, Fu G, Brooks PT, Weber I, Gadda G
  Title
Steady-state kinetic mechanism and reductive half-reaction of D-arginine dehydrogenase from Pseudomonas aeruginosa.
  Journal
Biochemistry 49:9542-50 (2010)
DOI:10.1021/bi101420w
Reference
5  [PMID:21707047]
  Authors
Fu G, Yuan H, Wang S, Gadda G, Weber IT
  Title
Atomic-resolution structure of an N5 flavin adduct in D-arginine dehydrogenase.
  Journal
Biochemistry 50:6292-4 (2011)
DOI:10.1021/bi200831a
  Sequence
[pae:PA3863]
Reference
6  [PMID:21999550]
  Authors
Yuan H, Xin Y, Hamelberg D, Gadda G
  Title
Insights on the mechanism of amine oxidation catalyzed by D-arginine dehydrogenase through pH and kinetic isotope effects.
  Journal
J Am Chem Soc 133:18957-65 (2011)
DOI:10.1021/ja2082729
Other DBs
ExplorEnz - The Enzyme Database: 1.4.99.6
IUBMB Enzyme Nomenclature: 1.4.99.6
ExPASy - ENZYME nomenclature database: 1.4.99.6
BRENDA, the Enzyme Database: 1.4.99.6
CAS: 37205-44-0
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