KEGG ENZYME: 1.5.7.1

ENZYME: 1.5.7.1

Entry

EC 1.5.7.1                  Enzyme

Name

methylenetetrahydrofolate reductase (ferredoxin);
5,10-methylenetetrahydrofolate reductase

Class

Oxidoreductases;
Acting on the CH-NH group of donors;
With an iron-sulfur protein as acceptor

Sysname

5-methyltetrahydrofolate:ferredoxin oxidoreductase

Reaction(IUBMB)

5-methyltetrahydrofolate + 2 oxidized ferredoxin = 5,10-methylenetetrahydrofolate + 2 reduced ferredoxin + 2 H+ [RN:R01217]

Reaction(KEGG)

R01217

Substrate

5-methyltetrahydrofolate [CPD:C00440];
oxidized ferredoxin [CPD:C00139]

Product

5,10-methylenetetrahydrofolate [CPD:C00143];
reduced ferredoxin [CPD:C00138];
H+ [CPD:C00080]

Comment

An iron-sulfur flavoprotein that also contains zinc. The enzyme from Clostridium formicoaceticum catalyses the reduction of methylene blue, menadione, benzyl viologen, rubredoxin or FAD with 5-methyltetrahydrofolate and the oxidation of reduced ferredoxin or FADH2 with 5,10-methylenetetrahydrofolate. However, unlike EC 1.5.1.53, methylenetetrahydrofolate reductase (NADPH); EC 1.5.1.54, methylenetetrahydrofolate reductase (NADH); or EC 1.5.1.20, methylenetetrahydrofolate reductase [NAD(P)H], there is no activity with either NADH or NADP+.

History

EC 1.5.7.1 created 2005, modified 2021

Pathway

ec00670

One carbon pool by folate

Reference

1 [PMID:6381490]

Authors

Clark JE, Ljungdahl LG.

Title

Purification and properties of 5,10-methylenetetrahydrofolate reductase, an iron-sulfur flavoprotein from Clostridium formicoaceticum.

Journal