KEGG   ENZYME: 1.8.1.22
Entry
EC 1.8.1.22                 Enzyme                                 
Name
dissimilatory sulfite reductase system;
siroheme sulfite reductase;
DsrABL;
hydrogen-sulfide:(acceptor) oxidoreductase (incorrect)
Class
Oxidoreductases;
Acting on a sulfur group of donors;
With NAD+ or NADP+ as acceptor
Sysname
[DsrC protein]-trisulfide,NAD+ oxidoreductase (sulfite-forming)
Reaction(IUBMB)
a [DsrC protein]-trisulfide + NAD+ + 3 H2O = a [DsrC protein]-dithiol + sulfite + NADH + H+ [RN:R00861]
Reaction(KEGG)
R00861;
(other) R00295 R08035
Substrate
[DsrC protein]-trisulfide;
NAD+ [CPD:C00003];
H2O [CPD:C00001]
Product
[DsrC protein]-dithiol;
sulfite [CPD:C00094];
NADH [CPD:C00004];
H+ [CPD:C00080]
Comment
Contains siroheme. The enzyme is essential in prokaryotic sulfur-based energy metabolism, including sulfate/sulfite reducing organisms, sulfur-oxidizing bacteria, and organosulfonate reducers. The system comprises the DsrAB reductase and the DsrL protein, which form a tight complex. The reaction involves the small protein DsrC, which is present in all the organisms that contain dissimilatory sulfite reductase. In sulfite reducers the DsrL component transfers two electrons from NADH to the DsrAB component, which then reduces the sulfur in sulfite to an S(II) intermediate that forms (together with two cysteine residues of DsrC) a trisulfide. In sulfur oxidizers the enzyme catalyses the opposite reaction [1].
History
EC 1.8.1.22 created 2015 as EC 1.8.99.5, transferred 2023 to EC 1.8.1.22
Pathway
ec00633  Nitrotoluene degradation
ec00920  Sulfur metabolism
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K11180  dissimilatory sulfite reductase alpha subunit
K11181  dissimilatory sulfite reductase beta subunit
Genes
TIG: THII_0611 THII_0612
THIS: HZT40_16500(dsrB) HZT40_16505(dsrA)
TUN: J9260_02800(dsrA) J9260_02805(dsrB)
TLO: J9253_11315(dsrB) J9253_11320(dsrA)
TSB: HMY34_03705(dsrB) HMY34_03710(dsrA)
TANI: J8380_00610(dsrB) J8380_00615(dsrA)
TWN: L2Y54_11490(dsrA) L2Y54_11495(dsrB)
TLC: RCF98_13395(dsrA) RCF98_13400(dsrB)
TDU: QJT80_01890(dsrA) QJT80_01895(dsrB)
ALV: Alvin_1251(dsrA) Alvin_1252(dsrB)
ATEP: Atep_14700(dsrA-2) Atep_14710(dsrB-1)
TVI: Thivi_0544 Thivi_0545
TMB: Thimo_0143 Thimo_0144
MPUR: MARPU_10910 MARPU_10915
TSY: THSYN_22540 THSYN_22545
TTP: E6P07_09385(dsrB) E6P07_09390(dsrA)
THIP: N838_27035(dsrB) N838_27040(dsrA)
CJAP: GWK36_02290(dsrA) GWK36_02295(dsrB)
THIM: KFB96_10675(dsrB) KFB96_10680(dsrA)
TBOG: LT988_06660(dsrB) LT988_06665(dsrA)
TLR: Thiosp_04057(dsrB) Thiosp_04058(dsrA)
TFRI: Thiofri_03978(dsrB) Thiofri_03979(dsrA)
TWG: Thiowin_00378(dsrA) Thiowin_00379(dsrB)
AEH: Mlg_1653 Mlg_1654
HHA: Hhal_1951 Hhal_1952
HHK: HH1059_16540(dsrA-2) HH1059_16550(dsrB-1)
TGR: Tgr7_2185 Tgr7_2186
TNI: TVNIR_0859(dsrB_[H]) TVNIR_0860 TVNIR_1766 TVNIR_1767(dsvB_[H])
TTI: THITH_11980 THITH_11985
TBN: TBH_C2461(dsrB) TBH_C2462(dsrA)
EPS: L0Y14_12180(dsrB) L0Y14_12185(dsrA)
SEDS: AAY24_10390 AAY24_17300 AAY24_17305
TTC: FOKN1_1306 FOKN1_1307 FOKN1_1945 FOKN1_1946
THIC: TspCOW1_07090(dsrB-1_1) TspCOW1_07100(dsrA-2_1) TspCOW1_13130(dsrB-1_2) TspCOW1_13140(dsrA-2_2)
SLIM: SCL_0256 SCL_0257 SCL_1273 SCL_1274
SVA: SVA_0258 SVA_0259 SVA_1954 SVA_1955
ACII: C4901_06765(dsrA) C4901_06770(dsrB)
ATY: A9R16_006355(dsrA) A9R16_006360(dsrB)
THO: SP60_01865 SP60_01870
REV: HUE57_11060(dsrB) HUE57_11065(dsrA)
RMA: Rmag_0869 Rmag_0870
REO: HUE58_04365(dsrB) HUE58_04370(dsrA)
VOK: COSY_0794(dsrB) COSY_0795(dsrA)
EBH: BSEPE_1260(dsrB) BSEPE_1261(dsrA)
BTHG: MS2017_1748 MS2017_1749
ENM: EBS_1783(dsrB) EBS_1784(dsrA)
IFO: CVFO_0512(dsrA) CVFO_0513(dsrB)
APHF: CVPH_1070(dsrB) CVPH_1071(dsrA)
RHOB: HTY51_04615(dsrB) HTY51_04620(dsrA)
HTN: KI616_02630(dsrB) KI616_02635(dsrA)
BBAG: E1O_03050 E1O_03060
SHD: SUTH_01443(dsrB) SUTH_01444(dsrA)
NPV: OHM77_04455(dsrA) OHM77_04460(dsrB)
TBD: Tbd_1309 Tbd_1369 Tbd_2484 Tbd_2485
SLT: Slit_1685 Slit_1686
SIDE: SIDCL21_1734
FAM: OYT1_ch1915 OYT1_ch1916
SEME: MIZ01_1475 MIZ01_1476
SDR: SCD_n02074(dsrA) SCD_n02717(dsrB) SCD_n02718(dsrA)
SLAC: SKTS_04680(dsrA-2_1) SKTS_33660(dsrB-1) SKTS_33670(dsrA-2_2)
DDZ: DSYM_11230 DSYM_11240
RVA: Rvan_0599 Rvan_0600
BLAG: BLTE_30830(dsrB-1) BLTE_30840(dsrA-2)
PAMO: BAR1_04755(dsrA) BAR1_04760(dsrB)
MGY: MGMSRv2__3911(dsrB) MGMSRv2__3912(dsrA)
MGRY: MSR1_25020(dsvA) MSR1_25030(dsvB)
MAGX: XM1_4244(dsrA) XM1_4245(dsrB)
MAGN: WV31_00295 WV31_00300
MAG: amb3367 amb3368
MAGQ: MGMAQ_2110(dsrA) MGMAQ_2111(dsrB)
VSU: P3M64_01050(dsrB) P3M64_01055(dsrA)
MGM: Mmc1_2156 Mmc1_2157
DAV: DESACE_07215 DESACE_07220
DDS: Ddes_0920 Ddes_0921 Ddes_2294 Ddes_2295
DFI: AXF13_14280 AXF13_14285
DPG: DESPIGER_1934 DESPIGER_1935 DESPIGER_1939 DESPIGER_1940
DEF: CNY67_09565(dsrA) CNY67_09570(dsrB) CNY67_15020(dsrB) CNY67_15025(dsrA)
DTR: RSDT_0579(dsrB) RSDT_0580(dsrA)
DFL: DFE_3070 DFE_3071
PSYF: N1030_11325(dsrA) N1030_11330(dsrB)
DSB: LN040_13680(dsrB) LN040_13685(dsrA)
DMA: DMR_03600 DMR_03610
DCB: C3Y92_18590(dsrB) C3Y92_18595(dsrA)
DGG: DGI_0689(dsrB) DGI_0691(dsrA)
DDE: Dde_0526 Dde_0527
DMS: E8L03_15015(dsrA) E8L03_15020(dsrB)
DSA: Desal_0787 Desal_0788
DHY: DESAM_20116(dsvB) DESAM_20117(dsvA)
DAF: Desaf_1370 Desaf_1371
DAS: Daes_2984 Daes_2985
DPI: BN4_12627(dsrA) BN4_12628(dsrB)
DEJ: AWY79_17895 AWY79_17900
PPRF: DPRO_1445(dsvB) DPRO_1446(dsvA)
PSEL: GM415_02165(dsrB) GM415_02170(dsrA)
DDN: DND132_2809 DND132_2810
PSEF: PSDVSF_27400(dsvA) PSDVSF_27410(dsvB)
PPOR: JCM14722_23130(dsvA) JCM14722_23140(dsvB)
PNW: SYK_28400(dsvA) SYK_28410(dsvB)
PTHE: LF599_15700(dsrB) LF599_15705(dsrA)
DSX: GD604_02670(dsrA) GD604_02675(dsrB)
DSD: GD606_09330(dsrB) GD606_09335(dsrA)
PBIZ: LWC08_10490(dsrB) LWC08_10495(dsrA)
DVU: DVU_0402(dsvA) DVU_0403(dvsB)
DVL: Dvul_2531 Dvul_2532
DVM: DvMF_1553 DvMF_1554
DVG: Deval_0364 Deval_0365
CLIH: KPS_001845(dsrA) KPS_001846(dsrB)
TMUR: JBF11_07875(dsrB) JBF11_07880(dsrA)
DBA: Dbac_0279 Dbac_0280
DOA: AXF15_07380 AXF15_07385
DESU: NLA06_11460(dsrB) NLA06_11465(dsrA)
THEW: TDMWS_09240(dsvA) TDMWS_09250(dsvB)
DRT: Dret_0244(dsrA) Dret_0245(dsrB)
DPS: DP0797 DP0798
DSF: UWK_01633 UWK_01634
DLM: DPPLL_01100 DPPLL_01110
DAK: DaAHT2_0296 DaAHT2_0297
DPR: Despr_2940 Despr_2941
DEO: CAY53_04490 CAY53_04495
DOG: HP555_06390(dsrA) HP555_06395(dsrB)
DBK: DGMP_00400(dsvA) DGMP_00410
DDU: GF1_25900 GF1_25910
ECOM: QTN59_15710(dsrA) QTN59_15715(dsrB)
DOL: Dole_2669 Dole_2670
DAL: Dalk_4300 Dalk_4301
DAT: HRM2_42390(dsrB2) HRM2_42400(dsrA2)
DTO: TOL2_C05570(dsrA) TOL2_C05580(dsrB)
DOV: DSCO28_40940(dsvA) DSCO28_40950(dsvB)
DWD: DSCW_41400(dsvA) DSCW_41410(dsvB)
DALK: DSCA_41750(dsvB) DSCA_41760(dsvA)
DML: Dmul_28170(dsrB) Dmul_28180(dsrA)
DLI: dnl_18160(dsrA) dnl_18170(dsrB)
DMM: dnm_073370(dsrB) dnm_073380(dsrA)
DEK: DSLASN_37970(dsvA) DSLASN_37980(dsvB)
DAO: Desac_1622 Desac_1623
DTI: Desti_1555 Desti_1556
SFU: Sfum_4042 Sfum_4043
DAX: FDQ92_05050(dsrB) FDQ92_05055(dsrA)
DBR: Deba_0959 Deba_0960
DMP: FAK_01560 FAK_01570(dsvB)
TID: Thein_0404 Thein_0405
TOP: TOPB45_1406 TOPB45_1407
TCM: HL41_05695
THET: F1847_01210(dsrA) F1847_01215(dsrB)
CTHI: THC_0449 THC_0450
TAV: G4V39_07740(dsrA) G4V39_07745(dsrB)
TMAI: FVE67_07900(dsrA) FVE67_07905(dsrB)
DHR: LGS26_00980(dsrA) LGS26_00985(dsrB)
DSY: DSY0309(dsrA) DSY0310(dsrB)
DHD: Dhaf_0252 Dhaf_0253
DDH: Desde_0266 Desde_0267
DDL: Desdi_0242 Desdi_0243
DOR: Desor_3302 Desor_3303
DAI: Desaci_3004 Desaci_3005
DMI: Desmer_2566 Desmer_2567
DCA: Desca_2665 Desca_2666
DRM: Dred_3186 Dred_3187
DRU: Desru_0386 Desru_0387 Desru_3723 Desru_3724
DFG: B0537_15840 B0537_15845
DAE: Dtox_0079 Dtox_0080
DKU: Desku_3492 Desku_3493
DAU: Daud_2200 Daud_2201
TFR: BR63_06745(dsrB) BR63_06750(dsrA)
FWA: DCMF_23740 DCMF_23745
AACX: DEACI_0668 DEACI_0669
DGI: Desgi_4661 Desgi_4662
CHY: CHY_2409(dsrB) CHY_2410(dsrA)
ADG: Adeg_2093 Adeg_2094
MTA: Moth_1600 Moth_1601 Moth_1629 Moth_1630
MTHO: MOTHE_c15990(dsvB1) MOTHE_c16000(dsvA1) MOTHE_c16350(dsvB2) MOTHE_c16360(dsvA2)
MTHZ: MOTHA_c16820(dsvB1) MOTHA_c16830(dsvA1) MOTHA_c17180(dsvB2) MOTHA_c17190(dsvA2)
EGD: GS424_009590(dsrB) GS424_009595(dsrA)
GPA: GPA_16670 GPA_16680
CBAC: JI75_04655 JI75_04660
TNR: Thena_1366 Thena_1367
TACI: TDSAC_1342 TDSAC_1343
CTE: CT0852(dsrA-1) CT0853(dsrB-1) CT2249(dsrA-2)
CPC: Cpar_0028 Cpar_0029
CLZ: BIU88_06835 BIU88_06840 BIU88_11280
CCH: Cag_1955 Cag_1956
CPH: Cpha266_0128 Cpha266_0129
CPB: Cphamn1_1856 Cphamn1_1857
CLI: Clim_0694 Clim_0695
PVI: Cvib_0039 Cvib_0040
PLT: Plut_0036 Plut_0037
PPH: Ppha_2320 Ppha_2321
PAA: Paes_0029 Paes_0030
PROC: Ptc2401_00028(dsvA) Ptc2401_00029(dsvB)
PRS: B9H02_00140
PROS: CHL67_00155(dsrA) CHL67_00160(dsrB)
PROW: OO006_03210(dsrA) OO006_03215(dsrB)
PROI: OO005_03115(dsrA) OO005_03120(dsrB)
TYE: THEYE_A1994(dsrA) THEYE_A1995(dsrB)
DTP: JZK55_23470(dsrB) JZK55_23480(dsrA)
AFU: AF_0423 AF_0424
AFG: AFULGI_00004270 AFULGI_00004280
APO: Arcpr_0139 Arcpr_0140
AVE: Arcve_1948 Arcve_1949
AST: Asulf_00915 Asulf_00916
PAI: PAE2571 PAE2572 PAE2596 PAE2597
PIS: Pisl_0170 Pisl_0171
PCL: Pcal_1445 Pcal_1446 Pcal_1456 Pcal_1457
PAS: Pars_0907 Pars_0908 Pars_1212 Pars_1213 Pars_1224
PYR: P186_1805 P186_1806 P186_1813 P186_1814
POG: Pogu_0993 Pogu_0994 Pogu_1014 Pogu_1015 Pogu_1026 Pogu_1027
TNE: Tneu_0344 Tneu_0345
CMA: Cmaq_0853 Cmaq_0854
TTN: TTX_0423(dsrA) TTX_0424(dsrA) TTX_0426(dsrA) TTX_0427(dsrA) TTX_1187(dsrB) TTX_1188(dsrA)
VDI: Vdis_2098 Vdis_2099
VMO: VMUT_0501 VMUT_0502
VSO: Vsou_25600 Vsou_25610
 » show all
Reference
1
  Authors
Schedel, M., Vanselow, M. and Trueper, H. G.
  Title
Siroheme sulfite reductase from Chromatium vinosum. Purification and investigation of some of its molecular and catalytic properties.
  Journal
Arch Microbiol 121:29-36 (1979)
Reference
2  [PMID:7338517]
  Authors
Seki Y, Sogawa N, Ishimoto M
  Title
Siroheme as an active catalyst in sulfite reduction.
  Journal
J Biochem 90:1487-92 (1981)
DOI:10.1093/oxfordjournals.jbchem.a133615
Reference
3  [PMID:9695921]
  Authors
Pott AS, Dahl C
  Title
Sirohaem sulfite reductase and other proteins encoded by genes at the dsr locus of Chromatium vinosum are involved in the oxidation of intracellular sulfur.
  Journal
Microbiology 144 ( Pt 7):1881-94 (1998)
DOI:10.1099/00221287-144-7-1881
  Sequence
[alv:Alvin_1251 Alvin_1252]
Reference
4  [PMID:18829451]
  Authors
Oliveira TF, Vonrhein C, Matias PM, Venceslau SS, Pereira IA, Archer M
  Title
The crystal structure of Desulfovibrio vulgaris dissimilatory sulfite reductase bound to DsrC provides novel insights into the mechanism of sulfate respiration.
  Journal
J Biol Chem 283:34141-9 (2008)
DOI:10.1074/jbc.M805643200
Reference
5  [PMID:24662917]
  Authors
Venceslau SS, Stockdreher Y, Dahl C, Pereira IA
  Title
The "bacterial heterodisulfide" DsrC is a key protein in dissimilatory sulfur metabolism.
  Journal
Biochim Biophys Acta 1837:1148-64 (2014)
DOI:10.1016/j.bbabio.2014.03.007
Reference
6  [PMID:31854015]
  Authors
Loffler M, Feldhues J, Venceslau SS, Kammler L, Grein F, Pereira IAC, Dahl C.
  Title
DsrL mediates electron transfer between NADH and rDsrAB in Allochromatium vinosum.
  Journal
Environ Microbiol 22:783-795 (2020)
DOI:10.1111/1462-2920.14899
  Sequence
[alv:Alvin_1251 Alvin_1252 Alvin_1259]
Other DBs
ExplorEnz - The Enzyme Database: 1.8.1.22
IUBMB Enzyme Nomenclature: 1.8.1.22
ExPASy - ENZYME nomenclature database: 1.8.1.22
BRENDA, the Enzyme Database: 1.8.1.22
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