Entry
Name
8-amino-8-demethylriboflavin N,N-dimethyltransferase;
rosA (gene name)
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
S-adenosyl-L-methionine:8-amino-8-demethylriboflavin N,N-dimethyltransferase
Reaction(IUBMB)
2 S-adenosyl-L-methionine + 8-amino-8-demethylriboflavin = 2 S-adenosyl-L-homocysteine + roseoflavin (overall reaction) [RN:
R11778 ];
(1a) S-adenosyl-L-methionine + 8-amino-8-demethylriboflavin = S-adenosyl-L-homocysteine + 8-demethyl-8-(methylamino)riboflavin [RN:
R11779 ];
(1b) S-adenosyl-L-methionine + 8-demethyl-8-(methylamino)riboflavin = S-adenosyl-L-homocysteine + roseoflavin [RN:
R11780 ]
Reaction(KEGG)
Substrate
S-adenosyl-L-methionine [CPD:
C00019 ];
8-amino-8-demethylriboflavin [CPD:
C21646 ];
8-demethyl-8-(methylamino)riboflavin [CPD:
C21648 ]
Product
S-adenosyl-L-homocysteine [CPD:
C00021 ];
roseoflavin [CPD:
C21647 ];
8-demethyl-8-(methylamino)riboflavin [CPD:
C21648 ]
Comment
The enzyme, characterized from the soil bacterium Streptomyces davawensis, catalyses the last two steps in the biosynthesis of the antibiotic roseoflavin.
History
EC 2.1.1.343 created 2017
Pathway
ec00998 Biosynthesis of various antibiotics
ec01110 Biosynthesis of secondary metabolites
Orthology
K22269 8-amino-8-demethylriboflavin N,N-dimethyltransferase
Genes
Taxonomy
Reference
Authors
Jankowitsch F, Kuhm C, Kellner R, Kalinowski J, Pelzer S, Macheroux P, Mack M
Title
A novel N,N-8-amino-8-demethyl-D-riboflavin Dimethyltransferase (RosA) catalyzing the two terminal steps of roseoflavin biosynthesis in Streptomyces davawensis.
Journal
Sequence
Reference
Authors
Tongsook C, Uhl MK, Jankowitsch F, Mack M, Gruber K, Macheroux P
Title
Structural and kinetic studies on RosA, the enzyme catalysing the methylation of 8-demethyl-8-amino-d-riboflavin to the antibiotic roseoflavin.
Journal
Sequence
Other DBs
ExPASy - ENZYME nomenclature database: 2.1.1.343
LinkDB
All DBs