Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
BRITE hierarchy

D-xylulose 5-phosphate:acyl-carrier protein glycolyltransferase

D-xylulose 5-phosphate + [acp] + a [lipoyl-carrier protein]-N6-[(R)-lipoyl]-L-lysine = D-glyceraldehyde 3-phosphate + glycolyl-[acp] + a [lipoyl-carrier protein]-N6-[(R)-dihydrolipoyl]-L-lysine (overall reaction) [RN:R13512];
(1a) D-xylulose 5-phosphate + a [lipoyl-carrier protein]-N6-[(R)-lipoyl]-L-lysine = D-glyceraldehyde 3-phosphate + a [lipoyl-carrier protein]-N6-[glycolyl-(R)-dihydrolipoyl]-L-lysine [RN:R13513];
(1b) a [lipoyl-carrier protein]-N6-[glycolyl-(R)-dihydrolipoyl]-L-lysine + [acp] = glycolyl-[acp] + a [lipoyl-carrier protein]-N6-[(R)-dihydrolipoyl]-L-lysine [RN:R13514]

R13512 R13513 R13514
Reaction

D-xylulose 5-phosphate [CPD:C00231];
acp [CPD:C00229];
[lipoyl-carrier protein]-N6-[(R)-lipoyl]-L-lysine;
[lipoyl-carrier protein]-N6-[glycolyl-(R)-dihydrolipoyl]-L-lysine [CPD:C23051]

This enzyme system produces glycolyl-[acp] units that can be used by non-ribosomal peptide synthases. It catalyses a transketolase-like reaction on ketose phosphates derived from primary metabolism and transfers the resulting glycolyl moiety to a dedicated acyl-carrier protein. During the reaction cycle the glycolyl moiety is transferred first to a thiamine diphosphate cofactor, then to a lipoyl cofactor, and eventually to the acyl-carrier protein. While D-xylulose 5-phosphate is the best substrate, the enzyme can also accept D-fructose 6-phosphate and D-sedheptulose 7-phosphate. During the reaction the lipoyl cofactor is reduced to dihydrolipoyl, which must be oxidized back to lipoyl by an unknown enzyme.

EC 2.3.1.330 created 2025

1  [PMID:22586110]

2  [PMID:30746093]