KEGG   ENZYME: 2.4.1.155
Entry
EC 2.4.1.155                Enzyme                                 
Name
alpha-1,6-mannosyl-glycoprotein 6-beta-N-acetylglucosaminyltransferase;
MGAT5 (gene name);
N-acetylglucosaminyltransferase V;
alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase;
uridine diphosphoacetylglucosamine-alpha-mannoside beta1->6-acetylglucosaminyltransferase;
UDP-N-acetylglucosamine:alpha-mannoside-beta1,6 N-acetylglucosaminyltransferase;
alpha-1,3(6)-mannosylglycoprotein beta-1,6-N-acetylglucosaminyltransferase;
GnTV;
GlcNAc-T V;
UDP-N-acetyl-D-glucosamine:6-[2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl]-glycoprotein 6-beta-N-acetyl-D-glucosaminyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
Sysname
UDP-N-acetyl-alpha-D-glucosamine:N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)-beta-D-mannosyl-glycoprotein 6-beta-N-acetyl-D-glucosaminyltransferase (configuration-inverting)
Reaction(IUBMB)
UDP-N-acetyl-alpha-D-glucosamine + beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein] = UDP + beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein] [RN:R05991]
Reaction(KEGG)
R05991(G);
(other) R04665
Substrate
UDP-N-acetyl-alpha-D-glucosamine [CPD:C00043];
beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein]
Product
UDP [CPD:C00015];
beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein]
Comment
Requires Mg2+. The enzyme, found in vertebrates, participates in the processing of N-glycans in the Golgi apparatus. It catalyses the addition of N-acetylglucosamine in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides, and thus enables the synthesis of tri- and tetra-antennary complexes.
History
EC 2.4.1.155 created 1986, modified 2001, modified 2018
Pathway
ec00510  N-Glycan biosynthesis
ec01100  Metabolic pathways
Orthology
K00744  alpha-1,3(6)-mannosylglycoprotein beta-1,6-N-acetyl-glucosaminyltransferase
Genes
HSA4249(MGAT5)
PTR459625(MGAT5)
PPS100989629(MGAT5)
GGO101129003(MGAT5)
PON100445523(MGAT5)
NLE100597446(MGAT5)
MCC709108(MGAT5)
MCF102123398(MGAT5)
CSAB103216975(MGAT5)
CATY105590337(MGAT5)
PANU100999378(MGAT5)
TGE112636563(MGAT5)
RRO104654400(MGAT5)
RBB108539646(MGAT5)
TFN117095089(MGAT5)
PTEH111527477(MGAT5)
CJC100414526(MGAT5)
SBQ101038866(MGAT5)
CSYR103271940(MGAT5)
MMUR105857612(MGAT5)
OGA100967000(MGAT5)
MMU107895(Mgat5)
MCAL110290015(Mgat5)
MPAH110321291(Mgat5)
RNO65271(Mgat5)
MCOC116098324(Mgat5)
MUN110551079(Mgat5)
CGE100760162(Mgat5)
PLEU114696950(Mgat5)
NGI103733594(Mgat5)
HGL101721991(Mgat5)
CPOC100712879(Mgat5)
CCAN109689552 109689553
DORD105996638(Mgat5)
DSP122110739(Mgat5)
NCAR124979610
OCU100346879(MGAT5)
OPI101529461(MGAT5)
TUP102497975(MGAT5)
CFA483895(MGAT5)
VVP112932611(MGAT5)
VLG121481799(MGAT5)
AML100483104(MGAT5)
UMR103667662(MGAT5)
UAH113263431(MGAT5)
UAR123782810(MGAT5)
ELK111141394
LLV125095948
MPUF101682188(MGAT5)
ORO101379628(MGAT5)
EJU114207232(MGAT5)
ZCA113919750(MGAT5)
MLX118012512(MGAT5)
FCA101088682(MGAT5)
PYU121030477(MGAT5)
PBG122479353(MGAT5)
PTG102969054(MGAT5)
PPAD109270081(MGAT5)
AJU106983003(MGAT5)
HHV120230385(MGAT5)
BTA537595(MGAT5)
BOM102275628(MGAT5)
BIU109568385(MGAT5)
BBUB102393516(MGAT5)
CHX102174983(MGAT5)
OAS101107672(MGAT5)
ODA120854416(MGAT5)
CCAD122454020(MGAT5)
SSC100512563(MGAT5)
CFR102521418(MGAT5)
CBAI105083402(MGAT5)
CDK105092441(MGAT5)
VPC102538273(MGAT5)
BACU102997655(MGAT5)
LVE103072240(MGAT5)
OOR101288542(MGAT5)
DLE111172696(MGAT5)
PSIU116757192(MGAT5)
ECB100050356(MGAT5)
EPZ103555913(MGAT5)
EAI106836533(MGAT5)
MYB102246850(MGAT5)
MYD102761898(MGAT5)
MMYO118661808(MGAT5)
MLF102427244(MGAT5)
MNA107531999(MGAT5)
PKL118709849(MGAT5)
HAI109374684(MGAT5)
DRO112313014(MGAT5)
SHON119002628(MGAT5)
AJM119041278
PDIC114493783(MGAT5)
PHAS123810334(MGAT5)
MMF118625823(MGAT5)
RFQ117026018(MGAT5)
PALE102884190(MGAT5)
PGIG120611924(MGAT5)
PVP105288371(MGAT5)
RAY107520333(MGAT5)
MJV108395954 108406913
TOD119255776(MGAT5)
SARA101542429(MGAT5)
LAV100664057(MGAT5)
TMU101345363
DNM101421414(MGAT5)
MDO100011845(MGAT5)
GAS123242284(MGAT5)
SHR100929992(MGAT5)
PCW110223747(MGAT5)
OAA100091600(MGAT5)
GGA424286(MGAT5)
PCOC116227423(MGAT5)
MGP100541307(MGAT5)
CJO107316876(MGAT5)
NMEL110399356(MGAT5)
APLA101797784(MGAT5)
ACYG106039337(MGAT5)
AFUL116490710(MGAT5)
TGU100228988(MGAT5)
LSR110480631(MGAT5)
SCAN103814283(MGAT5)
PMOA120498970(MGAT5)
OTC121336517(MGAT5)
PRUF121356506(MGAT5)
GFR102042048(MGAT5)
FAB101814359(MGAT5)
PHI102101886(MGAT5)
PMAJ107207494(MGAT5)
CCAE111932311(MGAT5)
CCW104694065(MGAT5)
ETL114062071(MGAT5)
ZAB102062481(MGAT5)
FPG101918414(MGAT5)
FCH102055854(MGAT5)
CLV102084918(MGAT5)
EGZ104129527(MGAT5)
NNI104009751(MGAT5)
ACUN113482013(MGAT5)
TALA104359020(MGAT5)
PADL103918490(MGAT5)
ACHC115342453(MGAT5)
AAM106498453(MGAT5)
AROW112977057(MGAT5)
NPD112948582(MGAT5)
DNE112988311(MGAT5)
ASN102386992(MGAT5)
AMJ102563315(MGAT5)
CPOO109322835(MGAT5)
GGN109301368(MGAT5)
PSS102455461(MGAT5)
CMY102946323(MGAT5)
CPIC101938965(MGAT5)
TST117884678(MGAT5)
CABI116826090(MGAT5)
MRV120374683(MGAT5)
ACS100566008(mgat5)
PVT110071860(MGAT5)
SUND121928031(MGAT5)
PBI103063095(MGAT5) 103064781
PMUR107284397(MGAT5)
TSR106545857(MGAT5)
PGUT117669472(MGAT5)
VKO123027373(MGAT5)
PMUA114604540(MGAT5)
ZVI118092382(MGAT5)
GJA107108668(MGAT5) 107111387
XLA108701278 108702251
XTR100145551(mgat5)
NPR108792298(MGAT5)
RTEM120944163(MGAT5)
BBUF121008578(MGAT5)
BGAR122944471(MGAT5)
DRE724006(mgat5)
SRX107739338 107745986
SANH107653587 107663499
SGH107586987 107593035(mgat5)
CCAR109047295 109083375 109087669
CAUA113040766 113064774 113077639
PPRM120486519(mgat5)
IPU108259240(mgat5)
PHYP113547435(mgat5)
SMEO124379668(mgat5)
TFD113657033(mgat5)
AMEX103037113(mgat5)
EEE113581466(mgat5)
TRU101072031(mgat5) 101074995
TNGGSTEN00006450G001 GSTEN00013473G001
LCO104925340(mgat5)
NCC104941679(mgat5) 104962910
CGOB115020149(mgat5) 115026505
ELY117249803(mgat5) 117251389
PLEP121949308 121962674(mgat5)
SLUC116037737 116044574(mgat5)
ECRA117939637(mgat5)
PFLV114551135 114563683
GAT120820174(mgat5) 120833820
PPUG119199315(mgat5) 119228632
MSAM119902006(mgat5) 119903256
CUD121506333(mgat5)
MZE101481161(mgat5)
ONL100691248(mgat5)
OAU116334403(mgat5)
OLA101168186(mgat5)
OML112159997(mgat5)
XMA102232481(mgat5) 102236488
XCO114140432(mgat5) 114148135
XHE116715581(mgat5) 116723023
PRET103460330 103476254(mgat5)
PFOR103141415(mgat5) 103152097
PLAI106936559 106948945(mgat5)
PMEI106903323 106914659
GAF122827248(mgat5) 122839913
CVG107094135 107094137(mgat5)
CTUL119771852 119789711(mgat5)
GMU124861575(mgat5)
NFU107381041 107390173(mgat5)
KMR108238485 108246486(mgat5)
ALIM106519126 106519129 106529442
NWH119419033 119428955(mgat5)
AOCE111562783 111582951
CSEM103389683(mgat5)
POV109631205 109639749(mgat5)
SSEN122761506 122785633
HHIP117754947 117775326(mgat5)
LCF108887100 108891873
SDU111218827 111222699(mgat5)
SLAL111646016(mgat5) 111667400
XGL120798018(mgat5) 120801806
HCQ109514989(mgat5)
BPEC110153275(mgat5)
MALB109955689(mgat5) 109968552
SASA106574313 106575089
OTW112253925 112253994
OMY110495947 110527561
OGO124008692 124010544
ONE115129626 115134299
SALP111953416 111971109
SNH120033306 120051232
ELS105019667(mgat5)
SFM108929747 108941965(mgat5)
PKI111849087(mgat5) 111849468
AANG118213644(mgat5) 118222127
LOC102687035(mgat5)
PSPA121322345 121322621
ARUT117407598(mgat5) 117426936
LCM102363158(MGAT5)
CMK103180379(mgat5) 103184928
RTP109917938 109920420 109923773
BFO118405638
BBEL109478949
CIN100175898 108949978
SCLV120340156 120342409 120342410 120347147
SPU100888694
APLC110989436
SKO100366560
CELCELE_C55B7.2(gly-2)
CBRCBG_12645(Cbr-gly-2)
NAINECAME_14592
TSPTsp_11593
HROHELRODRAFT_115589 HELRODRAFT_180890
LGILOTGIDRAFT_172419
PCAN112565538
BGT106078612
GAE121387511
HRF124149529
HRJ124253233
CRG105330629
MYI110458649
PMAX117317713
MMER123538281
OBI106880226
OSN115223971
LAK106160238 106160396 106171734 106172929 106177233 106181457
SMMSmp_040510 Smp_144260
OVIT265_04472
NVE5509104 5517864 5517897 5517929 5521677
EPA110232340 110232355 110232390 110241019 110241101 110244956
ATEN116288093 116288103 116295791 116306769 116308527 116308528
ADF107327239 107337307 107345019 107345720 107345743 107347453 107353933
AMIL114954488 114954490 114957136 114961709
PDAM113671483 113678068 113683774 113683775 113683780
SPIS111327556 111327561 111332554 111336901
DGT114517678 114520371 114522055 114527192
XEN124441138 124450082 124453287
HMG100200937 100214696
 » show all
Reference
1  [PMID:6216250]
  Authors
Cummings RD, Trowbridge IS, Kornfeld S.
  Title
A mouse lymphoma cell line resistant to the leukoagglutinating lectin from Phaseolus vulgaris is deficient in UDP-GlcNAc: alpha-D-mannoside beta 1,6 N-acetylglucosaminyltransferase.
  Journal
J Biol Chem 257:13421-7 (1982)
Reference
2  [PMID:2834054]
  Authors
Hindsgaul O, Tahir SH, Srivastava OP, Pierce M.
  Title
The trisaccharide beta-D-GlcpNAc-(1----2)-alpha-D-Manp-(1----6)-beta-D-Manp, as its 8-methoxycarbonyloctyl glycoside, is an acceptor selective for N-acetylglucosaminyltransferase V.
  Journal
Carbohydr Res 173:263-72 (1988)
DOI:10.1016/S0008-6215(00)90822-X
Reference
3  [PMID:1531335]
  Authors
Shoreibah MG, Hindsgaul O, Pierce M
  Title
Purification and characterization of rat kidney UDP-N-acetylglucosamine: alpha-6-D-mannoside beta-1,6-N-acetylglucosaminyltransferase.
  Journal
J Biol Chem 267:2920-7 (1992)
Reference
4  [PMID:8393437]
  Authors
Gu J, Nishikawa A, Tsuruoka N, Ohno M, Yamaguchi N, Kangawa K, Taniguchi N
  Title
Purification and characterization of UDP-N-acetylglucosamine: alpha-6-D-mannoside beta 1-6N-acetylglucosaminyltransferase (N-acetylglucosaminyltransferase V) from  a human lung cancer cell line.
  Journal
J Biochem 113:614-9 (1993)
DOI:10.1093/oxfordjournals.jbchem.a124091
Reference
5  [PMID:10395745]
  Authors
Park C, Jin UH, Lee YC, Cho TJ, Kim CH
  Title
Characterization of UDP-N-acetylglucosamine:alpha-6-d-mannoside beta-1,6-N-acetylglucosaminyltransferase V from a human hepatoma cell line Hep3B.
  Journal
Arch Biochem Biophys 367:281-8 (1999)
DOI:10.1006/abbi.1999.1252
  Sequence
[hsa:4249]
Reference
6  [PMID:11872751]
  Authors
Saito T, Miyoshi E, Sasai K, Nakano N, Eguchi H, Honke K, Taniguchi N
  Title
A secreted type of beta 1,6-N-acetylglucosaminyltransferase V (GnT-V) induces tumor angiogenesis without mediation of glycosylation: a novel function of GnT-V  distinct from the original glycosyltransferase activity.
  Journal
J Biol Chem 277:17002-8 (2002)
DOI:10.1074/jbc.M200521200
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.155
IUBMB Enzyme Nomenclature: 2.4.1.155
ExPASy - ENZYME nomenclature database: 2.4.1.155
BRENDA, the Enzyme Database: 2.4.1.155
CAS: 83588-90-3
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