Entry
Name
2'-phosphotransferase;
yeast 2'-phosphotransferase;
Tpt1;
Tpt1p;
2'-phospho-tRNA:NAD+ phosphotransferase
Class
Transferases;
Transferring phosphorus-containing groups;
Phosphotransferases with an alcohol group as acceptor
BRITE hierarchy
Sysname
2'-phospho-[ligated tRNA]:NAD+ phosphotransferase
Reaction(IUBMB)
2'-phospho-[ligated tRNA] + NAD+ = mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide
Substrate
2'-phospho-[ligated tRNA];
NAD+ [CPD:
C00003 ]
Product
mature tRNA;
ADP-ribose 1'',2''-phosphate [CPD:
C19851 ];
nicotinamide [CPD:
C00153 ]
Comment
Catalyses the final step of tRNA splicing in the yeast Saccharomyces cerevisiae [2]. The reaction takes place in two steps: in the first step, the 2'-phosphate on the RNA substrate is ADP-ribosylated, causing the relase of nicotinamide and the formation of the reaction intermediate, ADP-ribosylated tRNA [6]. In the second step, dephosphorylated (mature) tRNA is formed along with ADP ribose 1''-2''-cyclic phosphate. Highly specific for oligonucleotide substrates bearing an internal 2'-phosphate. Oligonucleotides with only a terminal 5'- or 3'-phosphate are not substrates [1].
History
EC 2.7.1.160 created 2006
Orthology
Genes
PMUR : 107296902 107299060
PTEX : 113452594 113452603
SANH : 107660200 107680919
CAUA : 113057677 113113728
PDAB : 135745643 135762575
PSWI : 130191627(trpt1) 130209314
ARUT : 117969806 117969834
DSI : Dsimw501_GD12992(Dsim_GD12992)
LSAX : 138952827 138952889 138952891
MYI : 110453825 110454377 110461815 110463278 110463684
PMAX : 117319789 117319790 117319793 117320750 117320751 117320752 117320759 117320760 117320761 117320762 117320763 117320764 117320765 117320766 117320767 117320768 117320769 117320770 117320771 117321790 117328414 117340441 117340572
AIRR : 138319576 138319577 138319578 138321803
MMER : 123535431 123559465
RPHI : 132713094 132713095 132728920 132728921
OSN : 115217002 115223462 115232388
ATH : AT2G45330(emb1067) AT5G23600
CSAT : 104708898 104768413 104783093 104784803 104789052 104793492
EUS : EUTSA_v10001570mg EUTSA_v10019720mg
BNA : 106396108 106450908 106450910 106450912 106453397
BOE : 106321472 106338018 106339903 106340025
MINC : 123215451 123223317
GRA : 105765757 105768641 105791653 105801017
GHI : 107900847 107917646 107932478 107942990 107944117
GMX : 100782234 100795797 100805163
CAM : 101503315 101504828 101505156
AHF : 112728253 112752841 112752900 112795090 112797076
PCOX : 137735245 137748805 137748806 137748808
SSTN : 125845280 125852800 125860276
SDUL : 129886687 129886689
NTA : 107773662 107788943 107808788
SMIL : 131008791 131020632
LSV : 111876933 111876934 111919931 111919932
CQI : 110694432 110711489 110713961 110721436
ATRI : 130810408 130820248
PSOM : 113275211 113322471 113339829
ATS : 109764554 109769434 109784468
TDC : 119287934 119308992 119356650
TAES : 123077035 123088167 123114593 123119298 123166055 123190590
MFLO : 136522038 136528477
PVIR : 120670393 120702505
ZOF : 122027121 122028537 122030811 122033342
PEQ : 110033203 110036057 110038771
AOF : 109822402 109827712 109846315
SMO : SELMODRAFT_108368 SELMODRAFT_111206 SELMODRAFT_112295 SELMODRAFT_122194 SELMODRAFT_125162 SELMODRAFT_137049 SELMODRAFT_35951 SELMODRAFT_35952 SELMODRAFT_403616 SELMODRAFT_404681 SELMODRAFT_77175 SELMODRAFT_85925 SELMODRAFT_94703 SELMODRAFT_98231
CVR : CHLNCDRAFT_133253 CHLNCDRAFT_138778
CGR : 2888972(GVI51_I07293)
NCS : NCAS_0C04770(NCAS0C04770)
NDI : NDAI_0G04120(NDAI0G04120)
TPF : TPHA_0P01070(TPHA0P01070)
TBL : TBLA_0B08270(TBLA0B08270)
TDL : TDEL_0D05360(TDEL0D05360)
KAF : KAFR_0C01470(KAFR0C01470)
KNG : KNAG_0K00640(KNAG0K00640)
LBG : 92207327(LODBEIA_P21310)
CAL : CAALFM_C300350WA(TPT1)
CAUR : CJI96_0004811 CJI96_0004871
ASAU : 88174405(PUMCH_003341)
YLI : 2909163(YALI2_C00028g)
NTE : NEUTE1DRAFT62747(NEUTE1DRAFT_62747)
SMP : 10809124(SMAC4_00071)
TTT : THITE_2090861 THITE_2120512
MTM : MYCTH_2304008 MYCTH_2307835
RTHE : 98121855(VTJ83DRAFT_1090) 98129193(VTJ83DRAFT_86)
TATV : 25777049(TrAtP1_000642)
TASP : 36617943(TrAFT101_001759)
CDET : 87937588(CDEST_01085)
ELA : UCREL1_10023 UCREL1_6793
SSL : SS1G_05079 SS1G_06954
BFU : BCIN_09g02300 BCIN_10g03170(Bctpt1)
PSCO : LY89DRAFT_577171 LY89DRAFT_709804
GLZ : GLAREA_00534 GLAREA_11874
CIM : CIMG_11231(CIMG03943)
PTRR : 6339757(PtrM4_005370)
ADAC : 96084784(ACET3X_004462)
CDEP : 91086443(L203_102231)
KMG : 30163801(I203_103011)
KNE : 92182004(IAR55_004746)
TASA : A1Q1_04188 A1Q1_06737
CCAC : CcaHIS019_0700100(TPT1)
LBC : LACBIDRAFT_153942 LACBIDRAFT_303457
CCI : CC1G_05759 CC1G_08347
ABP : AGABI1DRAFT100698(AGABI1DRAFT_100698) AGABI1DRAFT70670(AGABI1DRAFT_70670)
ABV : AGABI2DRAFT149451(AGABI2DRAFT_149451) AGABI2DRAFT206430(AGABI2DRAFT_206430)
MPR : MPER_05888 MPER_07090 MPER_08674 MPER_11696
MRR : Moror_11668 Moror_12691 Moror_2875 Moror_9803
MORE : E1B28_007152 E1B28_007176 E1B28_007216
PSTR : Pst134EA_005191 Pst134EA_005196
ACAN : ACA1_014870 ACA1_237270
PTM : GSPATT00032284001 GSPATT00034855001
LMAT : 92515018(LSCM1_05029)
LOI : 92361244(LSCM4_05367)
LEIN : 94188400(JIQ42_04749)
PHET : 94291593(JKF63_05559)
» show all
Taxonomy
Reference
Authors
Steiger MA, Kierzek R, Turner DH, Phizicky EM.
Title
Substrate recognition by a yeast 2'-phosphotransferase involved in tRNA splicing and by its Escherichia coli homolog.
Journal
Reference
Authors
Spinelli SL, Kierzek R, Turner DH, Phizicky EM
Title
Transient ADP-ribosylation of a 2'-phosphate implicated in its removal from ligated tRNA during splicing in yeast.
Journal
Sequence
Reference
Authors
Culver GM, McCraith SM, Consaul SA, Stanford DR, Phizicky EM
Title
A 2'-phosphotransferase implicated in tRNA splicing is essential in Saccharomyces cerevisiae.
Journal
Sequence
Reference
Authors
McCraith SM, Phizicky EM.
Title
An enzyme from Saccharomyces cerevisiae uses NAD+ to transfer the splice junction 2'-phosphate from ligated tRNA to an acceptor molecule.
Journal
J Biol Chem 266:11986-92 (1991)
Reference
Authors
Hu QD, Lu H, Huo K, Ying K, Li J, Xie Y, Mao Y, Li YY
Title
A human homolog of the yeast gene encoding tRNA 2'-phosphotransferase: cloning, characterization and complementation analysis.
Journal
Sequence
Reference
Authors
Steiger MA, Jackman JE, Phizicky EM.
Title
Analysis of 2'-phosphotransferase (Tpt1p) from Saccharomyces cerevisiae: evidence for a conserved two-step reaction mechanism.
Journal
Reference
Authors
Sawaya R, Schwer B, Shuman S.
Title
Structure-function analysis of the yeast NAD+-dependent tRNA 2'-phosphotransferase Tpt1.
Journal
Reference
Authors
Kato-Murayama M, Bessho Y, Shirouzu M, Yokoyama S.
Title
Crystal structure of the RNA 2'-phosphotransferase from Aeropyrum pernix K1.
Journal
Other DBs
ExPASy - ENZYME nomenclature database: 2.7.1.160
LinkDB
All DBs