KEGG   ENZYME: 3.5.1.12
Entry
EC 3.5.1.12                 Enzyme                                 
Name
biotinidase;
amidohydrolase biotinidase;
biocytinase;
biotin-amide amidohydrolase
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides
Sysname
biocytin amidohydrolase
Reaction(IUBMB)
biocytin + H2O = biotin + L-lysine [RN:R01077]
Reaction(KEGG)
R01077
Substrate
biocytin [CPD:C05552];
H2O [CPD:C00001]
Product
biotin [CPD:C00120];
L-lysine [CPD:C00047]
Comment
The enzyme, found in many bacterial species as well as animals, liberates biotin from biocytin and short biotinylated peptides, but not from biotinylated proteins. It also has activity on biotin esters and biotin amides.
History
EC 3.5.1.12 created 1961, modified 2023
Pathway
ec00780  Biotin metabolism
ec01100  Metabolic pathways
Orthology
K01435  biotinidase
Genes
HSA686(BTD)
PTR470760(BTD)
PPS100983934(BTD)
GGO101131296(BTD)
PON100458389(BTD)
NLE100584490(BTD)
HMH116462044(BTD)
MCC677722(BTD)
MCF101926326(BTD)
MTHB126946874
MNI105490406(BTD)
CSAB103241707(BTD)
CATY105578326(BTD)
PANU101018867(BTD)
TGE112617641(BTD)
MLEU105538149(BTD)
RRO104656449(BTD)
RBB108535861(BTD)
TFN117084205(BTD)
PTEH111539502(BTD)
CANG105505755(BTD)
CJC100409568(BTD)
SBQ101042865(BTD)
CIMI108299906(BTD)
CSYR103249273(BTD)
MMUR105872706(BTD)
LCAT123628581(BTD)
PCOQ105826206(BTD)
OGA100943917(BTD)
MMU26363(Btd)
MCAL110309042(Btd)
MPAH110325552(Btd)
RNO306262(Btd)
MCOC116084403(Btd)
ANU117706132(Btd)
MUN110543129 110551270(Btd)
CGE100755324(Btd)
MAUA101832998(Btd)
PROB127217006(Btd)
PLEU114708166(Btd)
MORG121455239(Btd)
MFOT126499146
AAMP119827426(Btd)
NGI103747521(Btd)
HGL101716226(Btd)
CPOC100723421(Btd)
CCAN109694811(Btd)
DORD105989900(Btd)
DSP122107949(Btd)
PLOP125358627(Btd)
NCAR124968278 124968503
MMMA107149653(Btd)
OCU100341187
OPI101524102(BTD)
TUP102476912(BTD)
GVR103595213(BTD)
CFA477059(BTD)
CLUD112660942(BTD)
VVP112925620(BTD)
VLG121478712(BTD)
NPO129507343(BTD)
AML100483327(BTD)
UMR103679387(BTD)
UAH113253913(BTD)
UAR123788358(BTD)
ELK111155099
LLV125104704
MPUF101679447(BTD)
MNP132011773(BTD)
MLK131824519(BTD)
NVS122908998(BTD)
ORO101380238(BTD)
EJU114205726(BTD)
ZCA113915824(BTD)
MLX118003387(BTD)
NSU110570752(BTD)
LWW102732709(BTD)
FCA101094289(BTD)
PYU121033648(BTD)
PCOO112849642(BTD)
PBG122492012(BTD)
PVIV125175576(BTD)
LRUF124526160
PTG102952175(BTD)
PPAD109258837(BTD)
PUC125921334
AJU106971609
HHV120238470(BTD)
BTA537669(BTD)
BOM102282711(BTD)
BIU109561800(BTD)
BBIS105000736(BTD)
CHX102179988(BTD)
OAS101113196(BTD)
BTAX128046105(BTD)
ODA120880376(BTD)
CCAD122445508(BTD)
MBEZ129549480(BTD)
SSC100737057(BTD)
CFR102506341(BTD)
CBAI105077624(BTD)
CDK105096612(BTD)
VPC102536252(BTD)
BACU103004341(BTD)
BMUS118893902(BTD)
LVE103080922(BTD)
OOR101278082(BTD)
DLE111168158(BTD)
PCAD102983963(BTD)
PSIU116753297(BTD)
NASI112401421(BTD)
ECB100063800(BTD)
EPZ103561484(BTD)
EAI106842762(BTD)
MYB102258405(BTD)
MYD102759604(BTD)
MMYO118668202(BTD)
MLF102419448(BTD)
PKL118715367(BTD)
EFUS103299853(BTD)
MNA107542412(BTD)
DRO112309951(BTD)
SHON118997593(BTD)
AJM119048309(BTD)
PDIC114502076(BTD)
PHAS123830655(BTD)
MMF118633287(BTD)
PPAM129088234(BTD)
HAI109395825(BTD)
RFQ117037188(BTD)
PALE102894216(BTD)
PGIG120582056(BTD)
PVP105288551 111739437(BTD)
RAY107508454(BTD)
MJV108403192(BTD)
TOD119256862(BTD)
SARA101555521(BTD)
SETR125997967(BTD)
LAV100663934(BTD)
TMU101354864
ETF101648355(BTD)
MDO100030538(BTD)
GAS123249200(BTD)
SHR100922840(BTD)
AFZ127539117
PCW110209057(BTD)
OAA100084040(BTD)
GGA420639(BTD)
PCOC116227086(BTD)
MGP100540232(BTD)
CJO107309167(BTD)
TPAI128086730(BTD)
LMUT125696481(BTD)
NMEL110394953(BTD)
APLA101796688(BTD)
ACYG106030251(BTD)
CATA118252780(BTD)
AFUL116486767(BTD)
TGU100226458(BTD)
LSR110474622(BTD)
SCAN103812912(BTD)
PMOA120502675(BTD)
OTC121339587(BTD)
PRUF121352913(BTD)
GFR102043957(BTD)
FAB101821386(BTD)
OMA130249061(BTD)
PHI102104565(BTD)
PMAJ107199918(BTD)
CCAE111923444(BTD)
CCW104689076(BTD)
CBRC103614134(BTD)
ETL114055684(BTD)
ZAB102075280(BTD)
ACHL103797008(BTD)
SVG106864282(BTD)
MMEA130573231(BTD)
HRT120760665(BTD)
FPG101914603(BTD)
FCH102056176(BTD)
CLV102085390(BTD)
EGZ104126131(BTD)
NNI104013909(BTD)
PCRI104036901(BTD)
PLET104623708(BTD)
EHS104504847(BTD)
PCAO104045793(BTD)
ACUN113476796(BTD)
TALA104359643(BTD)
PADL103918399(BTD)
AFOR103906634(BTD)
ACHC115339769(BTD)
HALD104323793(BTD)
HLE104843122(BTD)
AGEN126038078
GCL127013139
CCRI104156679(BTD)
CSTI104556663(BTD)
CMAC104483065(BTD)
MUI104542479(BTD)
BREG104641131(BTD)
FGA104072840(BTD)
GSTE104254419(BTD)
LDI104341838(BTD)
MNB103777184(BTD)
OHA104333320(BTD)
NNT104411675(BTD)
SHAB115618476(BTD)
DPUB104307530(BTD)
PGUU104467522(BTD)
ACAR104529906(BTD)
CPEA104397555(BTD)
AVIT104273902(BTD)
CVF104289893(BTD)
RTD128905284(BTD)
CUCA104059167(BTD)
TEO104375118(BTD)
BRHI104493186(BTD)
AAM106483475(BTD)
AROW112977278(BTD)
NPD112957226(BTD)
TGT104568400(BTD)
DNE112985275(BTD)
SCAM104145078(BTD)
ASN112549578
AMJ102566286(BTD)
CPOO109310413(BTD)
GGN109291601(BTD)
PSS102458076(BTD)
CMY102946514(BTD)
CCAY125631590(BTD)
DCC119850380(BTD)
CPIC101939105(BTD)
TST117872360(BTD)
CABI116837034(BTD)
MRV120398935(BTD)
ACS100556978(btd)
ASAO132778720(BTD)
PVT110072153(BTD)
SUND121934088(BTD)
PBI103068246(BTD)
PMUR107282909(BTD)
CTIG120307312(BTD)
TSR106545056(BTD)
PGUT117655107(BTD)
APRI131196704(BTD)
PTEX113441308(BTD)
NSS113415959(BTD)
VKO123033836(BTD)
PMUA114607098(BTD)
PRAF128398466(BTD)
ZVI118092351(BTD)
HCG128331692(BTD)
STOW125441284(BTD)
EMC129337494(BTD)
XLA108718912(btd.L)
XTR100489727(btd)
NPR108803235(BTD)
RTEM120940232(BTD)
BBUF121002205(BTD)
BGAR122938308(BTD)
MUO115460131(BTD)
GSH117354010(BTD)
DRE555376(btd)
SRX107732456(btd)
SANH107694759(btd)
SGH107559753
CCAR109080426(btd)
CAUA113062563 113062564
CGIB127935284
PTET122335192(btd)
LROH127181793(btd)
OMC131525513(btd)
RKG130078432(btd)
MAMB125275859 125276131
CIDE127501589
MASI127420874
TROS130567364(btd)
TDW130415564(btd)
MANU129455939(btd)
IPU108256913(btd)
IFU128600342(btd)
PHYP113536296(btd)
SMEO124375894(btd)
TFD113661654(btd)
TVC132863812(btd)
AMEX103037138
CMAO118806172(btd)
EEE113590652(btd)
CHAR105903673(btd)
TRU101069377(btd)
TFS130518580(btd)
TNGGSTEN00032985G001
LCO104939816 109139331 109139332
NCC104944686 104944699(btd)
TBEN117477819 117478235
CGOB115015920
PGEO117454682 117455173
GACU117562835 117562836
EMAC134874759(btd) 134874760
ELY117263564 117264124 117264125 117264127 117264131
EFO125904495 125905085 125905086 125905087 125905088 125905089 125905092 125905093 125905095
PLEP121958319(btd)
SLUC116066869 116066871 118493288(btd)
ECRA117956338 117956339(btd)
ESP116701988
PFLV114567763 114567944 114568312
GAT120826662(btd)
PPUG119218862(btd)
AFB129097277(btd)
CLUM117738939(btd)
PSWI130197291(btd)
MSAM119906734 119906832 119907789
SCHU122864009 122864010
CUD121523277 121523334 121523416 121524115 121524298 121524362
ALAT119014975 119014987
MZE101466121 101475752
ONL100694007 102082273(btd) 109194532 109197882 109198139 109198141 109200911 109200913 109200914 109204954
OAU116318428 116333647 116333669(btd) 116333671
OLA101173953
OML112136498 112136509
CSAI133461590(btd)
XMA102233822(btd)
XCO114155352(btd)
XHE116731274(btd)
PRET103472253 103472511
PFOR103134997 103135181
PLAI106936832(btd) 106943235
PMEI106922082 106922093
GAF122843529(btd)
PPRL129378484(btd)
CVG107085879
CTUL119785857(btd)
GMU124879401(btd)
NFU107383317
KMR108240453(btd)
ALIM106521953 106530461
NWH119425694(btd)
AOCE111573358(btd)
MCEP125019389(btd)
CSEM103394637(btd)
POV109642670(btd)
SSEN122786435(btd)
HHIP117770677 117770876 117770877
HSP118124941 118124942 118124943
SMAU118292177(btd)
LCF108877741 108877753
SDU111223068
SLAL111656913
XGL120786265(btd)
HCQ109527670(btd)
SSCV125987128
SBIA133496294(btd)
PEE133395457(btd)
PTAO133471163(btd)
BPEC110175184
MALB109968104
BSPL114865773(btd)
SJO128374148(btd)
SASA106570600 106588721 106588750
STRU115173883 115175361
OTW112240210(btd) 112264467
OMY110489374(btd) 110496519
OGO124011966 124012249 124043172
ONE115132671
OKI109879885 109908579
OKE118380688(btd) 127909650
SALP112074045(btd)
SNH120021968 120041045
CCLU121572271 121586635
ELS105012460(btd)
SFM108933388
PKI111846924(btd)
AANG118234546 118234547 118234548 118234549 118234550
LOC102695279(btd)
PSPA121315128(btd)
ARUT117394718(btd)
PSEX120515401(btd)
LCM102358398 102358664(BTD)
CMK103181262(btd)
RTP109911552 109933343
CPLA122550256(btd)
HOC132816079 132816203
LERI129710024(btd)
PMRN116948225(BTD)
LRJ133345176(BTD)
SPU575770
LPIC129269525
APLC110985242
DGR6565785
PTRU123518658
MNZ135204293
HAZT108668034
CSCU111631973 111642029
PTEP107449055 107449061
ABRU129962872 129963244 129981396 129981814 129981872 129981983
UDV129215989 129224917
SDM118201733 118203125
PMAX117333923
DPOL127831737 127869322 127875148
OSN115226121
XEN124446359
 » show all
Reference
1  [PMID:13211594]
  Authors
THOMA RW, PETERSON WH.
  Title
The enzymatic degradation of soluble bound biotin.
  Journal
J Biol Chem 210:569-79 (1954)
Reference
2  [PMID:14087327]
  Authors
KNAPPE J, BRUEMMER W, BIEDERBICK K.
  Title
[PURIFICATION AND PROPERTIES OF BIOTINIDASE FROM SWINE KIDNEY AND LACTOBACILLUS CASEI.]
  Journal
Biochem Z 338:599-613 (1963)
Reference
3  [PMID:5081874]
  Authors
Pispa J, Koivusalo M.
  Title
Actinomycin D-sensitive increase in the biotinidase activity in mouse liver and serum after ethionine feeding.
  Journal
Acta Chem Scand 26:2133-5 (1972)
DOI:10.3891/acta.chem.scand.26-2133
Other DBs
ExplorEnz - The Enzyme Database: 3.5.1.12
IUBMB Enzyme Nomenclature: 3.5.1.12
ExPASy - ENZYME nomenclature database: 3.5.1.12
BRENDA, the Enzyme Database: 3.5.1.12
CAS: 9025-15-4
LinkDB

DBGET integrated database retrieval system