KEGG   ENZYME: 4.1.99.2
Entry
EC 4.1.99.2                 Enzyme                                 
Name
tyrosine phenol-lyase;
beta-tyrosinase;
L-tyrosine phenol-lyase (deaminating)
Class
Lyases;
Carbon-carbon lyases;
Other carbon-carbon lyases
Sysname
L-tyrosine phenol-lyase (deaminating; pyruvate-forming)
Reaction(IUBMB)
L-tyrosine + H2O = phenol + pyruvate + NH3 (overall reaction) [RN:R00728];
(1a) L-tyrosine = phenol + 2-aminoprop-2-enoate [RN:R12759];
(1b) 2-aminoprop-2-enoate = 2-iminopropanoate (spontaneous) [RN:R11100];
(1c) 2-iminopropanoate + H2O = pyruvate + NH3 (spontaneous) [RN:R11099]
Reaction(KEGG)
Substrate
L-tyrosine [CPD:C00082];
H2O [CPD:C00001];
2-aminoprop-2-enoate [CPD:C02218];
2-iminopropanoate [CPD:C20904]
Product
phenol [CPD:C00146];
pyruvate [CPD:C00022];
NH3 [CPD:C00014];
2-aminoprop-2-enoate [CPD:C02218];
2-iminopropanoate [CPD:C20904]
Comment
A pyridoxal-phosphate protein. The enzyme cleaves a carbon-carbon bond, releasing phenol and an unstable enamine product that tautomerizes to an imine form, which undergoes a hydrolytic deamination to form pyruvate and ammonia. The latter reaction, which can occur spontaneously, can also be catalysed by EC 3.5.99.10, 2-iminobutanoate/2-iminopropanoate deaminase. The enzyme also slowly catalyses similar reactions with D-tyrosine, S-methyl-L-cysteine, L-cysteine, L-serine and D-serine.
History
EC 4.1.99.2 created 1972
Pathway
ec00350  Tyrosine metabolism
Orthology
K01668  tyrosine phenol-lyase
Genes
KQVB8P98_13910
KARLGL98_12615
CKOCKO_03467
CFDCFNIH1_09255
CBRAA6J81_13920
CWECO701_18800
CYOCD187_21020
CPOTFOB25_08080
CFQC2U38_02820
CIFAL515_04345
CIEAN232_00695
CPARCUC49_22445
CITZE4Z61_13950
CARSE1B03_04065
METYMRY16398_33370(tpl)
ASUBNLZ15_03930
KINAB182_14870
PDZHHA33_17405
EBCC2U52_25405
YMADA391_12585
PAMPANA_3984(tpl)
PLFPANA5342_p10091(tpl)
PAJPAJ_p0025(tpl)
PAQPAGR_p073
PMAGJI723_05445
MMKMU9_2951
PRAGEKN56_08045 EKN56_12310
LRINCTC12151_02412(tpl)
HPRPARA_06000
PMUPM0811
PMVPMCN06_0880(tpl)
PULNT08PM_0453
PMPPmu_08910(tpl)
PMULDR93_1650
PSKYA6B44_09725
AAPNT05HA_0124
AAZADJ80_05155
AATD11S_1940
AAOANH9381_0123
AAND7S_01988
AAHCF65_02970
AACNAANUM_2335
AACTACT75_02960
RPNENCTC8284_00313(tpl)
RHEYFEE42_07995
VMIAL543_07035
EAZJHT90_07445
MVSMVIS_2967(tnaA)
MYAMORIYA_1533(tnaA)
MCELLPW13_08815
KIMG3T16_05495
THIMKFB96_22295
ENWMJO57_30775
DNODNO_0442
CHJNCTC10426_00445(tpl)
AFFH3L97_00400
BGEBC1002_6700
PTXABW99_12265
CDNBN940_06946
COFFOZ74_12245
SIMPC6571_06710
RGERGE_20590(tnaA)
RBNRBXJA2T_17611(tnaA)
OFOBRW83_0351(tpl)
OVBNB640_06150
EBAebA605(tnaA)
APETToN1_35920
AZIAzCIB_0931
AZDCDA09_05540
TMZTmz1t_1789
TCLTchl_2494
TAKTharo_1668
CBLACBLAS_0788
ATPATR_1949(tnaA)
APOCAPORC_2028(tnaA)
ATHRATH_1913(tnaA)
NANNO0S08_25630
SATSYN_01455
RPBRPB_1966
RPCRPC_2787
RPDRPD_3423
RPERPE_2915
RPTRpal_4083
RPXRpdx1_1790
RVARvan_2623
BVRBVIR_1448
BLAGBLTE_20100(tpl)
MSCBN69_2860
MBRYB1812_17480
MROSEHO51_02135
MTWCQW49_09160
PSFPSE_0962(tnaA)
RSQRsph17025_3640
CAZTLV780_17545
RCPRCAP_rcc00516(tpl) RCAP_rcc02309(tnaA)
RSUNHU_02389(tnaA)
RHMB5V46_07690
HNEHNE_0810
RAPRHOA_5189(tpl)
RPMRSPPHO_03014
MAGXXM1_0733(tpl)
MAGNWV31_12030
BWDCT694_33960
BSHILGQ02_13810
SPAEE2C16_02905
SPOOJ3U78_00095
SURLBI350_06705
CTCCTC_00818
CTETBN906_00858
CCOHSAMEA4530647_0763(tpl_2)
CCAAKQH81_12795
CCLClocl_1892
HSCHVS_04190(tpl)
RHEREHE19_004455
CSHClosa_0252
LBWC3V36_04545
STHSTH2842
DSYDSY3653
DHDDhaf_1723
DDLDesdi_0330
DORDesor_4416
DFGB0537_00095 B0537_11110
ACAEHYG86_05650
TEPTepRe1_1876
TAETepiRe1_2021(tpl)
NTHNther_1802
NCDACONDI_01191(tpl_1) ACONDI_01327(tpl_2)
HPKHprae_1906
MHAPJFY71_03580 JFY71_06480
PUFUFO1_1418
PFTJBW_03623
LPILLIP_0995
TBKHF295_07920
CMDB841_11865(tnaA)
CHMB842_02140(tnaA)
CSPHCSPHI_06785
CCYSSAMEA4530656_1651(tnaA)
CFYI6L56_04290
CBQAL705_05405
SALUDC74_716
SALLSAZ_04135(tnaA)
SKACP970_07480
ICAIntca_0142
ARSADJ73_00465
AUSIPK37_00915 IPK37_04765
BRTJ4N02_01260 J4N02_04045
NCANoca_1624
AOIAORI_5366
AJAAJAP_22805(tpl)
AMYCCU254_28905
AMYYYIM_16040(tpl)
AORISD37_23450
AROONQK81_11530
RUBGBA63_15970
ERZER308_16190
CMPCha6605_2878
KOVK9N68_37855
LSKJ5X98_22205
NPUNpun_R5724
NOPNos7524_2080
NONNOS3756_28400
NOECLI64_22335
TOQHCG51_14585
RRSRoseRS_0320
RCARcas_0866
CAUCaur_0573
CHLChy400_0619
CAGCagg_3438
PSUBPelsub_P0466
PBFCFX0092_A2268(tnaA)
THUGKNN16_10440
DFCDFI_10615
OPROcepr_1553 Ocepr_1720
MHDMarky_1642
NPYNPRO_01750 NPRO_16190
OTEOter_0376
OBTOPIT5_12280
AMUAmuc_1995
SACISinac_1688
TDETDE_1118(tpl)
TPEDTPE_1499(tpl)
TPKJO40_08670
TPHGFUT81_00030
ABAAcid345_3931
ORPMOP44_23725
SUSAcid_6369
PFERIRI77_11825
ABACLuPra_04134(tpl)
THYDTTHT_1544(tnaA)
SCORJ3U87_27205
EMIEmin_0932
FNUFN1988
FNCHMPREF0946_02086
FNTHMPREF0405_01819
FUSHMPREF0409_01828
FNEFSDG_02356
FHWRN87_01430 RN87_05525
FPDCTM68_07230
FVAFV113G1_10500 FV113G1_23210
FULC4N20_00420 C4N20_11335
FNFBSQ88_03445
FPEIC4N17_00230
FCII6I83_00260
CSOMMKD34_06135
TAITaci_0642
PCRENCTC12858_00848(tpl_1)
PCAGNCTC12856_00106(tpl_1)
PENDLA319_03700
PSOECE91St14_06700(tpl)
PBTING2E5B_1329
PMUCING2E5A_1495(tpl)
PETPEIBARAKI_5473 PEIBARAKI_6464
PSACPSM36_1292 PSM36_2654(tpl)
PROJKDN43_00735 KDN43_09795
PPNPalpr_1828
ALQC7Y71_008285
ALDGFH31_00760
AITAI2BBH_02780(tpl)
RBCBN938_2021
DORIFH5T_08715
MCOSGM418_17880
ASXCDL62_16620
ALKQM9189_07190
SRUSRU_1708
SRMSRM_01915(tnaA)
RMRRmar_1948
RMGRhom172_0915
RBARAWN76_012460
COYHF329_00540 HF329_11305
HHYHalhy_0884
SMIZ4412673_01210(tpl_2)
SPHZE3D81_15330
LUTLupro_11550
AQDD1816_15070
WVIWeevi_1094
EGMAYC65_17205
ELTFGE20_04745
CHZCHSO_0681(tnaA)
CGNOK18_07025
CHHA0O34_09960
CIOCEQ15_17460
CPIPCJF12_15000
CARHEGY05_20955
CSHAEG350_19400
CNKEG343_13340
CGLENCTC11432_03144(tpl_1)
CTAK4412677_01735(tpl_2)
CSALNBC122_01726(tpl_2)
CSUPMTP09_03975
CCASEIB73_09735
KDAEIB71_04020
KFAQ73A0000_00315
EVAEIB75_11400
CNREB819_10635
EBVF0358_05840
IALIALB_2618(tnaA)
MROMROS_0698 MROS_1546
DTNDTL3_0081(tpl)
CABYCabys_2412
NMVNITMOv2_0067(tnaA)
NJANSJP_0536(tnaA)
NIFW02_04720(tnaA)
BANABARAN1_0718(tpl)
BIHBIP78_0966
THGTCELL_0288
PSYTDSAG12_02524(tpl)
 » show all
Reference
1  [PMID:4908868]
  Authors
Kumagai H, Yamada H, Matsui H, Ohkishi H, Ogata K.
  Title
Tyrosine phenol lyase. I. Purification, crystallization, and properties.
  Journal
J Biol Chem 245:1767-72 (1970)
Reference
2  [PMID:4908869]
  Authors
Kumagai H, Yamada H, Matsui H, Ohkishi H, Ogata K.
  Title
Tyrosine phenol lyase. II. Cofactor requrements.
  Journal
J Biol Chem 245:1773-7 (1970)
Other DBs
ExplorEnz - The Enzyme Database: 4.1.99.2
IUBMB Enzyme Nomenclature: 4.1.99.2
ExPASy - ENZYME nomenclature database: 4.1.99.2
BRENDA, the Enzyme Database: 4.1.99.2
CAS: 9059-31-8
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