KEGG   ENZYME: 4.2.1.172
Entry
EC 4.2.1.172                Enzyme                                 
Name
trans-4-hydroxy-L-proline dehydratase
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
Sysname
trans-4-hydroxy-L-proline hydro-lyase
Reaction(IUBMB)
trans-4-hydroxy-L-proline = (S)-1-pyrroline-5-carboxylate + H2O [RN:R11819]
Reaction(KEGG)
R11819
Substrate
trans-4-hydroxy-L-proline [CPD:C01157]
Product
(S)-1-pyrroline-5-carboxylate [CPD:C03912];
H2O [CPD:C00001]
Comment
The enzyme has been characterized from the bacterium Peptoclostridium difficile. The active form contains a glycyl radical that is generated by a dedicated activating enzyme via chemistry involving S-adenosyl-L-methionine (SAM) and a [4Fe-4S] cluster.
History
EC 4.2.1.172 created 2017
Pathway
ec00330  Arginine and proline metabolism
ec01100  Metabolic pathways
Orthology
K23121  trans-4-hydroxy-L-proline dehydratase
Genes
GSUGSU2101
GSKKN400_2127
GEBGM18_2359
GBMGbem_2499
GBNGEOBRER4_17200
GERKP004_08265
GSUBKP001_06890
GNTKP003_07795
GPLM1B72_12535
DBADbac_2366
DPSDP0616(pflD)
DATHRM2_04250(pflD1) HRM2_49330(pflD2)
DOVDSCO28_52190
DWDDSCW_54540
DALKDSCA_46900
DLIdnl_30770
DEKDSLASN_43020
HMRHipma_0362
DAVDESACE_04125
EGVEGCR1_16435
EAVEH197_03950
ERAFJ9537_19250
CBOCBO3183
CBACLB_3219
CBHCLC_3093
CBYCLM_3595
CBLCLK_2581
CBBCLD_1348
CBICLJ_B3455
CBFCLI_3322
CBMCBF_3314
CBJH04402_03270
CKLCKL_0323
CKRCKR_0275
CSQCSCA_0866
CLDCLSPO_c32860(pflD2)
CACECACET_c25680(pflD2)
CCKCcar_09825
CTYKCTK_C05150
CFMBJL90_19455
CARGRSJ17_07625
CDRKB9W14_14245
CFERD4Z93_05160
CCAAKQH81_14215
AMTAmet_0584
GFEGferi_16240
HHWNCTC503_02347(csdB_2)
CPRFK7H06_19485
PBIFKXZ80_10205
OVAOBV_40980
CAMLH6X83_10710
CCOMI6K69_04740
BYLA4V09_06310
BPROPMF13cell1_04098(cutC_3)
CSCIHDCHBGLK_00253(cutC_1)
CSOCLS_03180
DLOK5I24_04245
SINTK5I26_05885
CDFCD630_32820(pflD)
PDCCDIF630_03582
CDCCD196_3097(pflD)
CDLCDR20291_3143(pflD)
PDFCD630DERM_32820(pflD)
PSORRSJ16_12040
PHXKGNDJEFE_00506(cutC_2)
TEMJW646_00595
TEBT8CH_1917(pflD)
DORDesor_3978
DAIDesaci_1175
DMIDesmer_3087
DRMDred_2965
DRUDesru_0509
DFGB0537_08335
FWADCMF_25705
VGUHYG85_21430
ACAEHYG86_12305
CTHMCFE_0574
MDVC5Q96_03640
BPRMCL3_28280
TEPTepRe1_0044
TAETepiRe1_0046
NTHNther_2476
KMEH0A61_00345(cutC)
SEDZJYG23_11695
PUFUFO1_0399
PFTJBW_04226
MANAMAMMFC1_02761(bssA_3)
STEDSPTER_40410(cutC_6)
AARGAargi30884_27260(pflD_2)
ABSIA9CBEGH2_00460(pflD_1)
CIUG4D55_13890
ERBA4V01_16645
BSUEBS3272_00540
AMUCPan181_08370(bssA)
PBUL21SP3_00214(csdB)
PBPSTSP1_00751(csdB)
ALUSSTSP2_03489(bssA)
TAITaci_1437
TLITlie_1574
AMOAnamo_1952
CPORBED41_02450
BVUBVU_1421
BDOEL88_01945
BDHGV66_04255
PSACPSM36_1174
PDIBDI_0786
PARCCI960_07850
PMERINE87_00975(pflD)
PGOLK6V26_10100
PJOLK449_08315
OSPOdosp_3648
TTZFHG85_12835
BLQL21SP5_00958(bssA)
DORIFH5T_08275
DRCG0Q07_01065
MCOSGM418_11240
MBASALGA_0749
OCIFEZ18_06875
SHYRLA303_00540 LA303_03965
TLETlet_1141
CPOCOPRO5265_1565
DDFDEFDS_2103
DAPDacet_1516
GTLEP073_08865
TKOTK0289
TSITSIB_0631
TLTOCC_00382
ABIAboo_0169
ACFAciM339_0879
KCRKcr_0517
PSYTDSAG12_03102
 » show all
Reference
1  [PMID:28183913]
  Authors
Levin BJ, Huang YY, Peck SC, Wei Y, Martinez-Del Campo A, Marks JA, Franzosa EA, Huttenhower C, Balskus EP
  Title
A prominent glycyl radical enzyme in human gut microbiomes metabolizes trans-4-hydroxy-l-proline.
  Journal
Science 355:eaai8386 (2017)
DOI:10.1126/science.aai8386
Other DBs
ExplorEnz - The Enzyme Database: 4.2.1.172
IUBMB Enzyme Nomenclature: 4.2.1.172
ExPASy - ENZYME nomenclature database: 4.2.1.172
BRENDA, the Enzyme Database: 4.2.1.172
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