KEGG   ENZYME: 4.2.1.36
Entry
EC 4.2.1.36                 Enzyme                                 
Name
homoaconitate hydratase;
homoaconitase;
cis-homoaconitase;
HACN;
Lys4;
LysF;
2-hydroxybutane-1,2,4-tricarboxylate hydro-lyase (incorrect)
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
Sysname
(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate hydro-lyase [(Z)-but-1-ene-1,2,4-tricarboxylate-forming]
Reaction(IUBMB)
(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate = (Z)-but-1-ene-1,2,4-tricarboxylate + H2O [RN:R04371]
Reaction(KEGG)
R04371
Substrate
(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate [CPD:C05662]
Product
(Z)-but-1-ene-1,2,4-tricarboxylate [CPD:C04002];
H2O [CPD:C00001]
Comment
Requires a [4Fe-4S] cluster for activity. The enzyme from the hyperthermophilic eubacterium Thermus thermophilus can catalyse the reaction shown above but cannot catalyse the previously described reaction, i.e. formation of (R)-homocitrate by hydration of cis-homoaconitate. The enzyme responsible for the conversion of cis-homoaconitate into (R)-homocitrate in T. thermophilus is unknown at present but the reaction can be catalysed in vitro using aconitate hydratase from pig (EC 4.2.1.3) [2].
History
EC 4.2.1.36 created 1972, modified 2007
Pathway
ec00300  Lysine biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
ec01120  Microbial metabolism in diverse environments
Orthology
K01705  homoaconitate hydratase
Genes
QSU112018819 112023082
SCEYDR234W(LYS4)
SEUBDI49_0416
SPAOSPAR_D04320
AGOAGOS_ABL106C
ERCEcym_5123
KLAKLLA0_C15125g
KMXKLMA_30527(LYS4)
LTHKLTH0E10582g
VPOKpol_1031p57
ZROZYRO0A10076g
CGRCAGL0K10978g
NCSNCAS_0B02660(NCAS0B02660)
NDINDAI_0C05030(NDAI0C05030)
TPFTPHA_0L01150(TPHA0L01150)
TBLTBLA_0A01460(TBLA0A01460)
TDLTDEL_0B01800(TDEL0B01800)
TGBHG536_0H01840
KAFKAFR_0E02730(KAFR0E02730)
KNGKNAG_0A04240(KNAG0A04240)
ZMKHG535_0F05020
PPAPAS_chr4_0795
DHADEHA2F07238g
PICPICST_88705(LYS4)
PGUPGUG_01420
SPAASPAPADRAFT_58782(LYS4)
LELLELG_05659
CALCAALFM_C404410CA(LYS4)
CTPCTRG_03911
COTCORT_0B00840
CDUCD36_44080
CTENCANTEDRAFT_110241
YLIYALI0E02728g
CLUCLUG_00676
CLUSA9F13_17g00583
CAURCJI97_002417
SLBAWJ20_348(LYS4)
PKZC5L36_0B00600
BNNFOA43_001879(LYS4)
BBRXBRETT_004628(LYS4)
OPAHPODL_02077
SLUDSCDLUD_001925
NCRNCU08898(lys-6)
NTENEUTE1DRAFT58933(NEUTE1DRAFT_58933)
SMPSMAC_05116
PANPODANSg8469
TTTTHITE_2124288
MTMMYCTH_2301648
CTHRCTHT_0069590
MGRMGG_04842
PPEIPpBr36_03207
TMNUCRPA7_611
SSCKSPSK_03952
FGRFGSG_10949
FPUFPSE_06063
FVRFVEG_11644
FOXFOXG_12892
NHENECHADRAFT_103327 NECHADRAFT_78315 NECHADRAFT_95300
TRETRIREDRAFT_81570
TRRM419DRAFT_142874
MAWMAC_07556 MAC_09008
MAJMAA_04686 MAA_09361
CMTCCM_07793
PLJVFPFJ_10805
VALVDBG_00257
VDAVDAG_08540
CFJCFIO01_08629
CLUPCLUP02_07655
CHIGCH63R_04010 CH63R_10446
SAPOSAPIO_CDS2378 SAPIO_CDS2954
ELAUCREL1_5880
PFYPFICI_04889
SSLSS1G_01026
BFUBCIN_14g00430(Bclys4)
MBEMBM_05638
PSCOLY89DRAFT_738951
GLZGLAREA_01173
ANIAN6521.2
AFMAFUA_5G08890
ACTACLA_011660
NFINFIA_078420
AORAO090701000041
ANGANI_1_1106134(An15g00350)
AFVAFLA_004530
ALUCAKAW2_60650A(LYS4)
ACHEACHE_50239A(LYS4)
APUUAPUU_10764A(LYS4)
PCSPc16g06870
PDPPDIP_05800
TMFPMAA_011710
TRGTRUGW13939_08882
CIMCIMG_02639
CPWCPC735_037180
UREUREG_03117
PBLPAAG_03612
PBNPADG_06215
ABEARB_04155
TVETRV_03936
AJEHCAG_06854
BGHBDBG_09384
PNOSNOG_01131(SNOG_01129 SNOG_01130)
PTEPTT_19520
BZECOCCADRAFT_82932
BSCCOCSADRAFT_152920
BORCOCMIDRAFT_86014
AALTCC77DRAFT_1042638
ZTRMYCGRDRAFT_68535
PFJMYCFIDRAFT_202095
FFUCLAFUR5_03230
BCOMBAUCODRAFT_34146
NPAUCRNP2_10443
TMLGSTUM_00011608001
SPOSPAC343.16(lys2)
CNECNK00580
CNBCNBK2910
CNGCNAG_02565
CGICGB_K3700C
TMSTREMEDRAFT_44947
TASAA1Q1_04925
TVSTRAVEDRAFT_150870
DSQDICSQDRAFT_155148
PCOPHACADRAFT_252820
SHSSTEHIDRAFT_72757
HIRHETIRDRAFT_314980
PSQPUNSTDRAFT_66471
ADLAURDEDRAFT_112541
FMEFOMMEDRAFT_76165
GTRGLOTRDRAFT_107515
LBCLACBIDRAFT_184939
CCICC1G_14391
ABPAGABI1DRAFT59676(AGABI1DRAFT_59676)
ABVAGABI2DRAFT184036(AGABI2DRAFT_184036)
PCUBJR316_0006214
MRRMoror_4605
MOREE1B28_011496(LYS4_2)
SCMSCHCO_02541718(SCHCODRAFT_02541718)
CPUTCONPUDRAFT_119840
SLASERLADRAFT_468242
WSEWALSEDRAFT_59020
WICJ056_004489
UMAUMAG_10689
PFPPFL1_04410
MGLMGL_3298
MRTMRET_2395
MSYMMSY001_3108
PGRPGTG_09399
MLRMELLADRAFT_105205
MBRMONBRDRAFT_32374
SREPTSG_09627
ACANACA1_061650
PIFPITG_12462
PSOJPHYSODRAFT_562967
SPARSPRG_03993
NGRNAEGRDRAFT_80875
ABAAcid345_2965
IALIALB_1150
MROMROS_2220
CABYCabys_582
 » show all
Reference
1  [PMID:5954805]
  Authors
Strassman M, Ceci LN.
  Title
Enzymatic formation of cis-homoaconitic acid, an intermediate in lysine biosynthesis in yeast.
  Journal
J Biol Chem 241:5401-7 (1966)
Reference
2  [PMID:16524361]
  Authors
Jia Y, Tomita T, Yamauchi K, Nishiyama M, Palmer DR.
  Title
Kinetics and product analysis of the reaction catalysed by recombinant homoaconitase from Thermus thermophilus.
  Journal
Biochem J 396:479-85 (2006)
DOI:10.1042/BJ20051711
Reference
3  [PMID:10714900]
  Authors
Zabriskie TM, Jackson MD.
  Title
Lysine biosynthesis and metabolism in fungi.
  Journal
Nat Prod Rep 17:85-97 (2000)
DOI:10.1039/A801345D
Other DBs
ExplorEnz - The Enzyme Database: 4.2.1.36
IUBMB Enzyme Nomenclature: 4.2.1.36
ExPASy - ENZYME nomenclature database: 4.2.1.36
BRENDA, the Enzyme Database: 4.2.1.36
CAS: 9030-68-6
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