| Entry |
|
|---|
| Name |
|
|---|
| Class |
Isomerases;
Intramolecular transferases;
Transferring amino groups
|
|---|
| Sysname |
L-lysine 2,3-aminomutase
|
|---|
| Reaction(IUBMB) |
L-lysine = (3S)-3,6-diaminohexanoate [RN: R00461]
|
|---|
| Reaction(KEGG) |
|
|---|
| Substrate |
|
|---|
| Product |
(3S)-3,6-diaminohexanoate [CPD: C01142]
|
|---|
| Comment |
This enzyme is a member of the 'AdoMet radical' (radical SAM) family. It contains pyridoxal phosphate and a [4Fe-4S] cluster and binds an exchangeable S-adenosyl-L-methionine molecule. Activity in vitro requires a strong reductant such as dithionite and strictly anaerobic conditions. A 5'-deoxyadenosyl radical is generated during the reaction cycle by reductive cleavage of S-adenosyl-L-methionine, mediated by the iron-sulfur cluster. S-adenosyl-L-methionine is regenerated at the end of the reaction.
|
|---|
| History |
EC 5.4.3.2 created 1972
|
|---|
| Pathway |
|
|---|
| Orthology |
|
|---|
| Genes |
» show all
|
|---|
| Reference |
|
|---|
| Authors |
Zappia V, Barker HA. |
|---|
| Title |
Studies on lysine-2,3-aminomutase. Subunit structure and sulfhydryl groups. |
|---|
| Journal |
|
|---|
| Reference |
2 |
|---|
| Authors |
Aberhart, D.J., Lim, H.-J. and Weiller, B.H. |
|---|
| Title |
Stereochemistry of lysine 2,3-aminomutase. |
|---|
| Journal |
J Am Chem Soc 103:6750-6752 (1981) |
|---|
| Reference |
|
|---|
| Authors |
Frey PA |
|---|
| Title |
Lysine 2,3-aminomutase: is adenosylmethionine a poor man's adenosylcobalamin? |
|---|
| Journal |
|
|---|
| Reference |
|
|---|
| Authors |
Lieder KW, Booker S, Ruzicka FJ, Beinert H, Reed GH, Frey PA |
|---|
| Title |
S-Adenosylmethionine-dependent reduction of lysine 2,3-aminomutase and observation of the catalytically functional iron-sulfur centers by electron paramagnetic resonance. |
|---|
| Journal |
|
|---|
| Reference |
|
|---|
| Authors |
Lepore BW, Ruzicka FJ, Frey PA, Ringe D |
|---|
| Title |
The x-ray crystal structure of lysine-2,3-aminomutase from Clostridium subterminale. |
|---|
| Journal |
|
|---|
| Reference |
|
|---|
| Authors |
Frey PA, Reed GH |
|---|
| Title |
Pyridoxal-5'-phosphate as the catalyst for radical isomerization in reactions of PLP-dependent aminomutases. |
|---|
| Journal |
|
|---|
| Other DBs |
| ExplorEnz - The Enzyme Database: | 5.4.3.2 |
| ExPASy - ENZYME nomenclature database: | 5.4.3.2 |
| BRENDA, the Enzyme Database: | 5.4.3.2 |
|
|---|
| LinkDB |
|
|---|
