Ligases;
Forming carbon-nitrogen bonds;
Acid-D-ammonia (or amine) ligases (amide synthases)
BRITE hierarchy

[lipoyl-carrier protein]-L-lysine:lipoate ligase (AMP-forming)

ATP + (R)-lipoate + a [lipoyl-carrier protein]-L-lysine = a [lipoyl-carrier protein]-N6-(lipoyl)lysine + AMP + diphosphate (overall reaction)  [RN:R11143];
(1a) ATP + (R)-lipoate = lipoyl-AMP + diphosphate [RN:R07770];
(1b) lipoyl-AMP + a [lipoyl-carrier protein]-L-lysine = a [lipoyl-carrier protein]-N6-(lipoyl)lysine + AMP [RN:R07771]

R11143 > R12433 R12449;
R07770 R07771;
(other) R12429
Reaction

ATP [CPD:C00002];
(R)-lipoate [CPD:C16241];
[lipoyl-carrier protein]-L-lysine [CPD:C16240];
lipoyl-AMP [CPD:C16238]

Requires Mg2+. This enzyme participates in lipoate salvage, and is responsible for lipoylation in the presence of exogenous lipoic acid [7]. The enzyme attaches lipoic acid to the lipoyl domains of certain key enzymes involved in oxidative metabolism, including pyruvate dehydrogenase (E2 domain), 2-oxoglutarate dehydrogenase (E2 domain), the branched-chain 2-oxoacid dehydrogenases and the glycine cleavage system (H protein) [6]. Lipoylation is essential for the function of these enzymes. The enzyme can also use octanoate instead of lipoate.

EC 6.3.1.20 created 2006 as EC 2.7.7.63, transferred 2016 to EC 6.3.1.20