KEGG   ENZYME: 6.3.2.65
Entry
EC 6.3.2.65                 Enzyme                                 
Name
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-galactose:2-oxoglutarate ligase;
Pyl
Class
Ligases;
Forming carbon-nitrogen bonds;
Acid-D-amino-acid ligases (peptide synthases)
Sysname
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-galactose:2-oxoglutarate ligase (ADP-forming)
Reaction(IUBMB)
ATP + UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-galactose + 2-oxoglutarate = ADP + phosphate + UDP-yelosamine [RN:R13401]
Reaction(KEGG)
R13401
Substrate
ATP [CPD:C00002];
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-galactose [CPD:C22575];
2-oxoglutarate [CPD:C00026]
Product
ADP [CPD:C00008];
phosphate [CPD:C00009];
UDP-yelosamine [CPD:C22973]
Comment
The enzyme, discovered in the bacterium Bacillus cereus ATCC 14579, produces the unusual nucleotide sugar UDP-yelosamine, which is incorporated into the capsular polysaccharide of this organism.
History
EC 6.3.2.65 created 2024
Pathway
ec00541  Biosynthesis of various nucleotide sugars
ec01100  Metabolic pathways
Orthology
K27969  UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-galactose:2-oxoglutarate ligase
Genes
VOS: KNV97_17860
PCAR: PC2016_0418
MBUR: EQU24_06735
SIX: BSY16_5643
PDE: Pden_3552
HYO: NNO_0475
DSX: GD604_01465
BCE: BC5272
BTHV: CQJ30_17615
ROD: P8596_22365
SCA: SCA_2159
SSIF: AL483_11530
SCV: A4G25_09415
SPIC: SAMEA4384060_0385
SDB: CNQ82_02715
ESS: ATZ33_09725
VHY: G7082_02210
CIA: BEN51_02830
CIU: G4D55_18790
HKU: HIF96_00255
ERB: A4V01_21315
MTEU: R3I42_07065
NARO: CFH99_19655
DTN: DTL3_1013
 » show all
Reference
1  [PMID:25368324]
  Authors
Hwang S, Li Z, Bar-Peled Y, Aronov A, Ericson J, Bar-Peled M
  Title
The biosynthesis of UDP-d-FucNAc-4N-(2)-oxoglutarate (UDP-Yelosamine) in Bacillus cereus ATCC 14579: Pat and Pyl, an aminotransferase and an ATP-dependent Grasp protein that ligates 2-oxoglutarate to UDP-4-amino-sugars.
  Journal
J Biol Chem 289:35620-32 (2014)
DOI:10.1074/jbc.M114.614917
  Sequence
[bce:BC5272]
Other DBs
ExplorEnz - The Enzyme Database: 6.3.2.65
IUBMB Enzyme Nomenclature: 6.3.2.65
ExPASy - ENZYME nomenclature database: 6.3.2.65
BRENDA, the Enzyme Database: 6.3.2.65
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